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ICSI 프로그램에서 생쥐 투명대를 이용한 고환조직내 정자의 동결
서태광,전병균,류은경,이은숙,류재웅,손시환,문진수,김광철,Suh, Tae-Kwang,Jeon, Byeong-Gyun,Ryu, Eun-Kyung,Lee, Eun-Sook,Ryoo, Zae-Yoong,Sohn, Sea-Hwan,Moon, Jin-Soo,Kim, Kwang-Chull 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.2
The survival rate and motility recovered after cryopreservation of testicular spermatozoa in testicular sperm extraction (TESE)-ICSI program is low. The purpose of this study was to assess the availability and efficiency of mouse empty zona pellucida in cryopreserving human TESE spermatozoa. Mouse empty zonae pellucidae were obtained by extraction of cytoplasm with or without cytochalasin B treatment. Motile sperm from proven-fertile donor and two azoospermic patients after TESE were individually inserted into empty zona pellucida and cryopreserved. Two to five days after cyropreservation, the frozen sperm were thawed and the rates of recovery and motility were observed. The ooplasmic extraction rates of control (N=80) and cytochalasin B treated oocytes (N=80) were 94.0% and 96.2%, respectively (p>0.05). The post-thaw recovery rates of spermatozoa and rates of motility recovery of ejaculate (N=70) and testicular (N=70) sperm were 97.1%, 97.1% and 95.7%, 94.3%, respectively (p>0.05). The results of this study showed that the mouse zone pellucida is useful for cryostorage of single testicular spermatozoa.
허태광(Heo TaeGwang),서정렬(Suh JeongYeal) 한국주거환경학회 2016 주거환경(한국주거환경학회논문집) Vol.14 No.2
This study started from the natural principle that feng shui characteristics affect the stability of human life and analyzed the correlation between feng shui and the stability of residential life. While applying the principle of yangtaek feng shui in the study of feng shui characteristics of the topography of Busan and their synthetically expressions, this paper moved away from the existing method and made a new approach focusing on energy field theory. First of all, to understand the elements of economic stability, considering the distinguishing standard of the principle of yangtaek, it analyzed the sample group of 1,532 households in 583 apartment complexes in Busan. It used not only the principle of yangtaek, Korea’s traditional location theory, but the modified, modern scientific features of yangtaek principle and analyzed the deciding factors of apartment prices using hedonic technique. As a result of positive analysis, characteristics analysis of houses and complexes, the south-facing houses, brand apartments, houses close to subway stations, etc. turned out to be an important variable. Also, in terms of stability level of residential life, the analysis conducted with the consideration of the indexes of crime rate, fire incidences, disease incidences, medical expenses, the intention to move and the area hoped to move to as the result of synthetic expression depending on the topographical characteristics of feng shui, showed that the stability of feng shui corresponded with the stability of residential life. From this study, the feng shui approach was found to be important in determining the appropriate and inappropriate places for housing in urban area. It was found that the stability characteristics of feng shui affect the stability of living.
생쥐에 있어서 兩分分割球 移植에 의한 새끼生産에 관한 硏究
徐泰光,朴恒均 慶北大學校 農業科學技術硏究所 1988 慶北大農學誌 Vol.6 No.-
本 硏究는 생쥐에 대한 過批卵反應, 回收된 受精卵의 兩分率, 兩分된 分割球의 體外培養 및 移植後 産仔로의 發達率을 조사하기 위하여 CBA 및 C57BL 系統의 供試싱쥐에서 얻어진 受精卵을 0.5% protease로 투명대제거 및 미세유리봉으로 兩分하여 얻어진 分割球를 blastocyst로 培養하여 BALB/C 系統의 受卵생쥐에 移植하여 다음과 같은 結果를 얻었다. hCG 注射後 36∼42時間, 48∼54時間, 62∼66時間, 72∼78時間에 受精卵을 回收한 結果 各各 2細胞期, 4細胞期, 8細胞期 및 桑實胚期의 受精卵이 대부분 回收되었으며 首當 平均 17.0個의 卵子가 回收되었다. 回收後 兩分한 8細胞期 受精卵의 兩分率은 83.6%로서 桑實胚의 65.5%에 比해 유의적으로 높았으며 分割球의 發達率은 桑實胚에서 얻어진 分割球가 76.5%로서 8細胞期 分割球의 60.9%에 比해 유의적으로 높았다. 한편 培養된 分割球와 intact embryo에 있어서 細胞期에 따라 各各 受卵생쥐當 2個, 10個, 15個씩 移植하였을 때 共히 15個씩 移植한 경우 受胎率이 가장 높았으나 細胞期에 따른 受胎率에 있어서 유의적인 차이는 없었다. 培養된 分割球와 intact embryo를, 桑實胚에서도 intact embryo를 移植하였을때 새끼 生産率은 各各 37.6% 및 43.4%로서 各各 培養된 分割球를 移植하였을때 보다 새끼生産率이 유의적으로 높았다. This study was conducted to investigate the effect of embryo stage on bisection rates of embryos, on development of separated demi-embryos and on subsequent development to full term following transfer of demi-embryos to recipients. The results obtained in this study are summarized as follows. The 2-, 4-, 8-cell and morula embryos were mostly obtained at 36-42, 48-54, 62-66 and 72-78 hours after injection of hCG, respectively, and the average number of embryos recovered per head was 17.0. The bisection rate of 8-cell embryos was 83.6%, which was significantly higher than that of morula embryos, 65.5%. But the development of morula demi-embryos to blastocyst after in-vitro culture was better than that of 8-cell demi-embryos and the rate was 76.5% and 60.9%, respectively. When the 2, 10 and 15 cultured demi-embryos or intact embryos were transferred to each recipient mouse, respectively. the highest pregnancy rate was obtained when 15 embryos were transferred. And the overall offspring production rate of intact embryos was higher than that of demi-embryos.
이정호,서태광,박항균 韓國受精卵移植學會 1987 한국동물생명공학회지 Vol.2 No.1
This study was carried out in order to find out better ways for superovuiation and egg collection by checking some factors affecting on donor cows such as iactating and dried, age, season of treatment and sequence of treatment. The results were summarized as follows:1. Number of corpus luteum and collected eggs of eactating and dried doner were 9.8 vs 8.0 and 9.6 vs 7.9,respectively. However, the rate of transferable embryos of lactating doner were higher than that of dried, 82.5% vs 48.1%. 2. The average number of corpus luteum and collected eggs of lactating donors under 7 years of age (6.7 vs lactating 5.3) were slightly lower than those of over 8 years of age (11.1 vs 9.2). But the rate of transferable embryoswas better in under 7 years old donors than over 8 years (81.1% vs 49.3%). were 6.0, 4.8, 1.5, 1.8 and 75%, and those in the summer were 2.9, 3.8, 2.2 and 46.7%, respectively. 3. The average number of corpus luteum was the highest in winter (10.5) and the rate of egg collection was the best in autumn (94.7%). The rate of the transferable embryo was the highest in winter (64.3%). 4. The average number of corpus luteum was the lowest (5.5) in the animals treated six or more superovulating treatments. The rate of egg collection was the best in the third treatment group (90.2%), but it was getting worse after fifth treatment, The rate of transferable embryos was the highest in the second treatment group (94.1%), and it was decreased thereafter.
박영식,서태광,이택후,전상식 韓國受精卵移植學會 1995 한국동물생명공학회지 Vol.10 No.3
This study was carried out to efficiently use the ultrarapid freezing method in the cryopreservation of mouse ova. For this, the effects of dehydration method, oval vigour and controlling method on post-thawing viability were investigated. Fresh mouse ova were dehydrated in mPBS with 3.5M DMSO and /or 0.25M sucrose, and directly immersed in L for ultrarapidly freezing. The frozen ova were thawed at 37, rehydrated in mPBS with 0.25M sucrose, and then repeatedly washed in HAM's Fl0 before evaluating the morphological normality of frozen-thawed ova. The results obtained showed that there was difference between treatments in a experiment. 1) The post-thawing viability of ova dehydrated in multi-step (48.413.8%) was higher than that of ova in two-step (40.914.0%). 2) The post-thawing viability of fertilized ova (8714.0%) was significantly(p<0.0l) higher than that of unfertilized ova (5.45.4%). 3) The post-thawing viability of ova dehydrated and rehydrated using a cooling machine (95.84.2%) was significantly(p<0.05) higher than that on ice(84.19.9). In conclusion, in order to efficiently cryopreserve ova in vitro with ultrarapidly freezing method, highly viable embryos should be selected, heavy osmotic shock to the dehydrating ova should be avoided, and embryos in high osmotic condition were dehydrated and rehydrated in a constantly low temperature.