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HCT116 대장암세포에서 Akt/Mdm2 신호 경로 조절을 통한 영릉향 헥산 분액물(LFH)의 Apoptosis 유도 효과 확인
서지원,이효재,이창열,김상용,김영민 한국생물공학회 2021 KSBB Journal Vol.36 No.4
Lysimachiae foenum-graeci Herba is a shade-dried whole plants of the Lysimachia foenum-graecum Hance and is well known to have anti-oxidant, anti-obesity and antibacterial effects. Accordingly, in this study, we assessed the apoptotic effects of hexane fractions from Lysimachiae foenum-graecum (LFH) on HCT116 Human colon cancer cells, and to confirm whether this effects are due to the regulation of Akt/Mdm2 signal pathways. As a result of MTT assay, LFH showed cell proliferation inhibitory and cytotoxic effects of HCT116 cells, and confirmed through Annexin V & Dead Cell assay to the staining rate of phosphatidylserine (PS) expressed by apoptosis. In addition, it was confirmed through Hoechst 33342 assay that the fluorescence caused by apoptosis progression and apoptosis bodies were caused by apoptosis induction of HCT116 cells by LFH. And it was confirmed through Western blotting that treatment of LFH induced inhibition of p-Akt, p-Mdm2, Bcl-2, and Pro-caspase 3 and activation of p-AMPK, p53, Bak, Bax, and Cleaved-caspase 3. Furthermore, it was confirmed that the apoptosis induction effect by LFH was caused by Akt/Mdm2 pathway regulation through Akt inhibitor (LY294002) and Mdm2 inhibitor (Nut- lin-3) treatment. In conclusion, it was confirmed that LFH induces apoptosis through Akt/Mdm2 signaling pathway in HCT116 Human colon cancer cells.
서지원,이동근,홍종철,박헌수 대한이비인후과학회 2014 대한이비인후과학회지 두경부외과학 Vol.57 No.5
Adenolipoma of the thyroid gland, also called thyrolipoma or thyroid hamartoma, is an extremely rare neoplasm. It is characterized by encapsulated admixtures of mature adipose tissue and thyroid tissue. The treatment of choice is surgical resection and the prognosis is favorable. We describe a case of adenolipoma of the thyroid gland, incidentally found in a residual gland subsequent to thyroidectomy of a papillary carcinoma. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(5):348-50