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서정현,( Li Lin ),여주상,차형준 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.1
Baculoviral polyhedrin, which originated from Autographa califonica nuclear polyhedrosis virus, was employed for the first time as a novel fusion partner for expression of foreign proteins in Escherichia coli system. We characterized the expression of recombinant polyhedrin protein fused to green fluorescent protein (GFP). The polyhedrin fusion protein was successfully expressed as an insoluble inclusion body. The E. coli expressing polyhedrin-GFP fusion protein showed higher cell growth and higher GFP yield than the strain expressing soluble single GFP. Interestingly, the polyhedrin fusionportion showed almost the same characteristics as the native baculoviral polyhedrin; it was rapidly solubilized under alkaline conditions, similar to the conditions found in the insect midgut. In addition, the polyhedrin fusion portion was rapidly digested by alkaline proteases in insect Plutella xylostella midgut as well as by α-chymotrypsin, a protease that has similar properties to insect midgutpolyhedra-associated alkaline proteases. These unique properties suggest that baculoviral polyhedrin might be an advantageous fusion partner for production of foreign proteins, especially harmful proteins, in E. coli.
서정현,김지현,주성후,Seo, Jung-Hyun,Kim, Ji-Hyun,Ju, Sung-Hoo 한국전기전자재료학회 2014 전기전자재료학회논문지 Vol.27 No.2
To operate organic light emitting device (OLED) with alternating current (AC) power source without AC/DC(direct current) converter, we fabricated the fluorescent OLED and measured the emission characteristics with AC and DC. The OLED operated by AC showed higher maximum current efficiency of 8.2 cd/A and maximum power efficiency of 8.3 lm/W. But current efficiency and power efficiency of AC driven OLED showed worse than DC driven OLED at high voltage above 10 V. This result can be explained by the peak voltage of AC was $\sqrt{2}$ times than DC, In case of low driving voltage the emission characteristics were improved by the peak voltage of AC, but in case of high driving voltage the emission efficiencies were decreased by the roll off phenomena. Finally, serial OLED arrays using twelve OLEDs driven by AC 110 V showed average voltage of 9.17 V, voltage uniformity of 99.0%, average luminance of $1,175cd/m^2$, luminance uniformity of 94.4%.