http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Modulation of Inflammatory Responses to Enhance Nerve Recovery after Spinal Cord Injury
서영권 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.3
Inflammation can occur at the wound site, and immune cells are necessary to trigger wound healing and tissue regeneration after injury. It is partly initiated by the rapid migration of immune cells such as neutrophils, inflammatory monocytes, and macrophages after spinal cord injury (SCI). Secondary inflammation can increase the wound area; thus, the function of tissues below the injury levels. Monocytes can differentiate into macrophages, and the macrophage phenotype can change from a pro-inflammatory phenotype to an anti-inflammatory phenotype. Therefore, various studies on immunomodulation have been performed to suppress secondary inflammation upon nerve damage. This editorial commentary focuses on various therapeutic methods that modulate inflammation and promote functional regeneration after SCI.
Reinforced Bioartificial Dermis Constructed with Collagen Threads
서영권,윤희훈,박장서,송계용,박정극 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.6
In this work, a novel type of composite scaffold was designed, which has the suitability of both high biocompatibility and strong mechanical properties, for use in bioartificial dermis applications. The reinforced scaffold consisted of a lyophilized collagen sponge formed around a cross-linked collagen meshwork with an average thread diameter of approximately 55 μm. Fibroblasts were cultured in the reinforced collagen sponge for 7 days, during which time the pores in the sponge became filled with cells that secreted extracellular matrix (ECM) to form a bioartificial dermis. Results of ultimate tensile strength (UTS) measurements and compression tests indicated that the bioartificial dermis formed around the reinforced collagen sponge showed about ten times the strength of the bioartificial dermis formed around a typical collagen sponge (1.5 ± 0.05 vs. 0.15 ± 0.05 and 2.5 ± 0.1 vs. 0.2 ± 0.08 MPa, respectively). As a result, reinforced collagen mesh improved mechanical properties and this technique will be possible to make stronger scaffolds, not only for artificial skin applications but also various artificial tissues, such as synthetic cartilage, bone, and blood vessels.
P-438 : A Standardized Assay for Test of Pigmentation
서영권,신연호,이수연,송계용,양은경,박정극 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.2
Effects of chemical or biological compounds on mammalian pigmantation have been reported by many researcher. Many melanocyte or artificial skin models have been used to evaluate the potential efficacy of melanogenic compounds to regulate pigmentation. However, in the skin and hair, melanocytes interaction with keratinocyte, fibroblasts, and other cell types, and testing of compounds on melanocytes alone in culture does not allow one to observe the interactions with those other cell types, such as wound occur in vivo. We have developed a model that use immotalized murin melanocytes(melana) or melanocyte in pure / coculture. We developed a melana or mlanocyte-human skin keratinocyte pure / coculture protocol that allows testing of compounds for potential effects on pigmentation. We have standardized it with known melanogenic inhibitor (arbutin) and stimulator (α-melanocyte stimulating hormone, UVB-irridation). After treatment of melanocytes with bioactive compounts, cell viability, total melanin contents, and tyrosinase activity are measured.
Manufacturing of collagen membrane for bioartificial skin
서영권,박정극,송계용,서성준,김영진 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.1
Human keratinocytes can be growen reliably and reproducibility in vitro to form multilayered epithelium. These sheets of cultured keratinocytes have been used to patients with severe burns andleg ulcers. There is a delay of 2 to 3 weeks for culture of the sheets of keratinocytes. So we made an artificial skin comprised of a keratinocyte in skin and a type I collagen membrane. In this work, we made a collagen membrane for the purpose of scaffold for artificial skin. And we showed primary culture keratinocyte, and cultured on the collagen membrane. The collage membrane could be obtained using 5 mg/ml collagen solution and lyopilization and then dry methods. To develop the artificial skin 500,000 cells/cm2 of keratinocyte were cultured on the collagen membrane. Consequentely, it is possible that to make the bioartificial skin within 10 days.
가교결합된 콜라젠 기질의 물리적 특성과 체내 염증반응의 상관관계
서영권,신연호,조현우,박정극,송계용 한국조직공학과 재생의학회 2011 조직공학과 재생의학 Vol.8 No.1s
To study the correlation of physical properties and degradation rate of cross-linked collagen matrix by chemicals in vivo, non-crosslinked collagen matrix, carbodiimide-crosslinked collagen matrix, and glutaraldehydecrosslinked collagen matrix were implanted in rat subcutaneous tissue. Change of structure was evaluated by scanning electron microscopy, degradation, differential scanning calorimetry, and tensile strength were evaluated in vitro,while inflammatory cell infiltration and degradation degree by light microscopy and transmission-electron microscopy after implantation in vivo. Result of differential scanning calorimeter estimation showed increased the denaturation temperature from 83.87oC to 112.81oC after glutaraldehyde treatment and to 114.56oC after carbodiimide treatment. And both crosslinked collagen matrixes were more resistant than the non-crosslinked collagen matrix in vitro degradation test with collagenase. The average breaking strength of the non-crosslinked matrix was 0.01 ± 0.005 MPa,whereas strength of the glutaraldehyde-crosslinked matrix was 0.029 ± 0.005 N. After implantation of the matrix in the subcutaneous tissue of rat, more infiltration of neutrophils was noted in glutaraldehyde-crosslinked matrix than in carbodiimide-crosslinked matrix at day 10 and day 21. Major difference was the degradation rate of the matrix,showing that non-crosslinked matrix was degraded about 90% after 21 days, whereas crosslinked matrix remained about 70~80%. This in vivo study revealed that degradation rate of the implanted matrix was much more depend upon the inflammatory reaction of chemicals for crosslink of matrix rather than matrix itself. It is concluded that the difference between in vitro and in vivo physical property and its longevity of matrix in implanted sites was closely related with induced inflammatory reaction to the matrix itself and crosslinking chemicals.
서영권 ( Young Kwon Seo ),안재일 ( Jae Il Ahn ),이두훈 ( Doo Hoon Lee ),권순용 ( Soon Yong Kwon ),정도현 ( Do Hun Jung ),박용순 ( Yong Soon Park ),송계용 ( Kye Yong Song ),양은경 ( Eun Kyung Yang ),김영진 ( Young Jin Kim ),박정극 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2
Amniotic membrane (AM) dressing can be used for the protection against superficial bacterial infection, facilitation of reepithelialization, reduction of caloric and fluid loss from burn or ulcers. In addition, it increases the speed of healing and minimizes the scarring of tissue repair of wounds. Objective of this study is to evaluate the wound healing effect of deepithelialized human AM with keratinocytes on it and the usefulness as substrates of epithelial cells culture. Deepithelialized AM was made by removal of their amniotic epithelial cells with 0.05% trypsin treatment and lyophilization and compared the wound healing effect with the intact human AM with keratinocytes. First, we evaluated the antiinflammatory effect of human AM, which was mixed with collagen solution in a powder form and freeze dried. Second, we cultured keratinocytes onto deepithelialized AM and grafted to the rat skin defect in full-thickness, and then the epidermal regeneration and dermal fibrosis was compared. As results, AM showed antiinflammatory effects evidence by much lowered inflammatory cell infiltrates in the implantation sites. Keratinocytes were cultured better and stratified on the deepithelialized AM than intact AM. Epithelial regeneration and fibrosis was also much better than intact AM. Therefore, we can conclude that AM had antiinflammatory effects, improve the wound healing and also suitable for cell culture substrates.