Novel Peptide Nucleic Acid Melting Array for the Detection and Genotyping of Toxoplasma gondii

서수환,윤한승,이상은,곽효선 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4

Despite differences in virulence between strains of Toxoplasma gondii, rapid and accurate genotyping methods are lacking. In this study, a method was developed to detect and genotype T. gondii in food and environmental samples using PCR and a novel peptide nucleic acid (PNA) melting array. An alignment of genome sequences for T. gondii type I, II, and III obtained from NCBI was generated, and a single nucleotide polymorphism analysis was performed to identify targets for PCR amplification and a PNA melting array. Prior to the PNA melting array, conventional PCR was used to amplify GRA6 of T. gondii. After amplification, the PNA melting array was performed using two different PNA hybridization probes with fluorescent labels (FAM and HEX) and quenchers. Melting curves for each probe were used to determine genotypes and identify mutations. A 214-bp region of the GRA6 gene of T. gondii was successfully amplified by PCR. For all T. gondii strains (type I, II, and III) used to evaluate specificity, the correct genotypes were determined by the PNA melting array. Non-T. gondii strains, including 14 foodborne pathogens and 3 protozoan parasites, such as Giardia lamblia, Cryptosporidium parvum, and Entamoeba histolytica, showed no signal, suggesting that the assay has a high specificity. Although this is only a proof-of-concept study, the assay is promising for the fast and reliable genotyping of T. gondii from food and environmental samples.

水蛭이 Streptozotocin으로 유발된 Rat의 糖尿病性 腎症에 미치는 影響

서수환(Su-Hwan Seo),김용성(Young-Seong Kim),한양희(Yang-Hee Han) 대한한의학회 2008 대한한의학회지 Vol.29 No.4

Fourier Ptychographic Microscopy의 Stitching 기법을 적용한 WSI 이미징 연구

서수환(SooHwan Seo),임진상(JinSang Lim),한완희(WanHee Han),박노철(No-cheol Park) 한국생산제조학회 2021 한국생산제조시스템학회 학술발표대회 논문집 Vol.2021 No.7

북서여수해만의 해양환경과 식물플랑크톤 생물량의 시?공간적 변화와 기초생산자의 성장제한 영양염의 변화

윤양호,서수환 전남대학교 수산과학연구소 2013 수산과학연구소논문집 Vol.21 No.-

To investigate spatio-temporal variations in marine environments such as water types and nutrients (nitrogen, phosphorus and silicon) and phytoplankton biomass in the northwestern area of Yeosuhae bay, as well as variations of limiting factor for phytoplankton growth, this researcher conducted a field survey for four seasons of 2012. According to the results of the survey, the marine environment of Yeosuhae bay showed that the water in winter and spring water had low temperature and high salinity, the water in summer had high temperature and high salinity and the water in fall had high temperature and low salinity. The water quality in the bay changed drastically depending on the season; however, nitrogen and silicon were provided by waters from inland and phosphorate levels were strongly affected by a nearby industrial complex. Variations in phytoplankton biomass as chlorophyll a concentration in the present study area, compared to those in Korean southwester sea, was found to be high in winter and summer and low in spring and fall. In particular, the fall survey which was conducted right after the occurrence of a large typhoon, indicated many differences from the general marine environments. The standing crop in spring was higher than that in winter, but biomass in spring was lower. As a result, no systematic relations between standing crop and biomass were found. In addition, during the time when a coastal industrial complex in Gwangyang bay near Yeosuhae bay was first built and while it was operating (i,e., between the late 1970s and the 200s), the growth-limiting nutrient for phytoplankton was nitrogen, but now it was found to be phosphorus. In other words, the phosphate that was abnormally high in density during the time when the complex was operating was found to be improved through the management of the quality of waters flowing into Gwangyang bay and the change to the industrial structure of the complex. It is believed that these results will be able to be used as good examples in managing the water quality in eutrophied bays.

육미지황탕(六味地黃湯)이 Alloxan으로 유발(誘發)된 생쥐의 당뇨병성(糖尿病性) 신증(腎症)에 미치는 영향(影響)

김용성,서수환,Kim, Yong-Sung,Seo, Soo-Hwan 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.4

This study was done to investigate recovery effects of YJT, which has been used clinically in diabetes therapy. Mice were administerd Alloxan to induce diabetes. There body weight and kidney weight changes, BUN, creatinine, glucose, total protein and ALP activity in serum, urinalysis, excretion volume of Na+, K+ in urine were measured. The results were as follows: 1. Increase in body weight and kidney weight and the ratio of kidney to body weight of YJT treated group showed similar results with those of normal group. 2 The BUN, creatinine, ALP activity levels in serum of YJT treated group were similar with those of normal group. 3. The glucose level in serum of YJT treated group was better than that of control group 4. The result of the urinalysis in the group of YJT was almost same as that of control group The above results suggest that YJT partially improves the function of the kidney.

유통식육에서의 톡소포자충 검출을 위한 유전자검사법 개발

윤한성,서수환,곽효선,주인선 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.3

Although many PCR-based assays have been developed, the majority of rapid detection of Toxoplasma gondii in animal and their meat product has been dependent on immunogenic assays. Thus, there is still a need for more reliable PCR based detection method for T. gondii in retail meats. Recently, a 529-bp repeat element that exists in 200-300 copies per genome of T. gondii genome had been spotlighted for its usefulness as potential detection targers. In this study, the 529-bp repeat element was selected for real-time PCR to detect three types of T. gondii (type I, II and III). A primer pair targeting 82-bp of the 529-bp element detected all three types of T. gondii and showed high level of specificity against 14 different food-borne pathogens as well as 3 protozoan parasites such as Giardia lamblia, Cryptosporidium parvum and Entamoeba histolytica. Application of the new real-time PCR assay in meat samples showed improved detection sensitivity compared to the B1-gene targeted method suggesting potential new target for Toxoplasma gondii screening in retail meats.

소아(小兒) 야뇨증(夜尿症)의 치료(治療)에 관한 임상적(臨床的) 고찰(考察)

이경환,서수환,김행진,김용성,Lee Kyung-Hwan,Seo Soo-Hwan,Kim Hang-Jin,Kim Yong-Sung 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.1

Between February 2002 and June 2003, A clinical study on Noctural Enurosis Patients had been conducted by the Department of Internal medicine at Dongshin University Oriental hospital. In this study, we treated nine patients with ear acupuncture using seed of Allium tuberosum ROTTLER and Herbal medicine. And we got the efficacious results. Moreover, the patients who were treated for more than 20days show more efficient result.

탁주 제조 시 율무 첨가에 따른 휘발성 성분의 분석

신순영(Soon-Young Shin),서수환(Soo-Hwan Suh),조원대(Won Dai Cho),이효구(Hyo Ku Lee),황한준(Han-Joon Hwang) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.8

탁주의 기능성 강화를 위하여 율무를 첨가하여 제조한 탁주의 탁주로서의 적합성을 조사하였다. 우선 기존 쌀코오지를 이용하여 전분원으로서 율무를 15, 30, 60와 100% 첨가했을 경우, 효모의 균수와 ethanol의 생성량은 각각 9.5×107~2.3×10^8 cell/mL과 13.6~15.2%로 시료 간의 큰 차이를 보이지 않아 율무의 알콜 발효원으로 적합성을 보여주었다. 한편 고급알콜인 iso-amyl alcohol은 첨가된 율무량에 따라 1,150ppm으로부터 1,206, 1,213, 1,293, 1,604 ppm으로 각각 증가되고, acetaldehyde와 iso-butanol도 율무의 첨가량에 따라 증가하였다. 한편 Aspergillus kawachii(AK)와 Rhizopus japonicus(RJ)를 이용하여 율무누룩주를 만든 결과 ethanol 생성량은 쌀코오지로 만든 율무주에 비해 14%로부터 각각 11.2%(AK), 7.5%(RJ) 그리고 10.4%(AK+RJ)로 감소하였고, 효모의 수는 7.5×10^7~2.6×10^8 cell/mL이었으며, isoamyl alcohol 함량이 1,840 ppm으로부터 각각 632[AK], 855[RJ], 792 ppm[AK+RJ]로 감소하였고, n-propanol, isobutanol 등의 fusel oil도 감소하였으나, acetaldehyde가 57 ppm으로부터 99[AK], 138[RJ], 131 ppm[AK+RJ]으로 각각 증가되었다. 전체적으로 탁주제조에 있어 율무의 이용은, 전분원으로서 사용하는 경우 알콜 발효와 관능검사에는 큰 영향을 미치지 않았으나 fusel oil의 함량이 증가하였고, 누룩을 원료로서 사용하는 경우 ethanol의 생성과 fusel oil의 생성이 감소하고, acetaldehyde의 함량이 현격히 증가하였으며, 관능검사 결과 기호도가 낮아졌다. In order to use nutraceutical components in Yulmoo (Coix lachryma-jobi L. var. ma-yuen Stapf), the acceptability of Yulmoo for Takju (Korean rice wine) was examined in terms of the production of volatile components. When Yulmoo was added to the Takju made by commercial koji as a substitute of starch at 0 (rice 100%), 15, 30, 60, and 100%, the ethanol content slightly increased to 13.6, 15.2, 15,2, 14.1, and 13.8%; the production of iso-butanol and iso-amyl alcohol increased as the amount of Yulmoo increased. While the use of Yulmoo to the Takju as the nuruk (Korean traditional koji), inoculated with Aspergillus kawachii (AK) and Rhizopus japonicus (RJ), decreased the contents of n-propanol, iso-butanol, and iso-amyl alcohol as well as the contents of ethanol from 14.3% to 11.2 [AK], 7.5 [RJ], and 10.4% [AK+RJ]. The Yulmoo nuruk in Takju increased the production of acetaldehyde and ethyl acetate indicating the negative effect in the organoleptic evaluation. It was determined that the addition of Yulmoo as a starch source or as nuruk has a critical effect on the production of fusel oils and other volatile compounds in Takju fermentation.

대학도서관 경영역량강화를 위한 전략적 계획 모델 제시

김남숙,김선미,김승환,서수환,유완식,정혜미 한국사립대학교 도서관협의회 2013 사대도협회지 Vol.14 No.-

본 연구의 목적은 현재 전략적 계획을 진행 중 또는 계획 중인 대학도서관들에게 모델을 제시하는 것에 있다. 이를 위해, 첫째, 설문조사방식으로 한국사립대학도서관협의회(이하 ‘사대도협’) 회원 120개 도서관의 전략적 계획에 대한 인식을 조사하였다. 둘째, 사대도협, 국공립대학도서관협의회 회원 도서관 및 2011 QS 대학평가 Top 50 대학도서관의 홈페이지 조사를 통해 사명, 비전, 전략 공개 현황을 조사.분석하였다. 셋째, 실질적인 모델을 제안하기 위하여 국내·외 대학도서관 전략의 세부과제를 유형별로 분류하여 제시하였다. 조사결과 우리나라의 일부 대학도서관은 신축, 조직개편 등을 목적으로 도서관 발전전략을 수립하여 학내에 공표하고 있으나, 학외에 공개하는 경우는 미미하다. 국내 대학도서관의 전략계획서에는 연구·학습·강의 지원을 위한 서비스 개발, 전통적인 도서관 공간 서비스, 이용자들의 문화적 욕구 충족 서비스, 특성화분야 콘텐츠 구축, 독서진흥 프로그램 개발 등을 주로 다루고 있다. 반면, 해외 대학도서관은 미래를 위한 도서관 공간계획, 연구·교육·학습을 지원할 새로운 IT 개발, 학생 경험 관련 서비스 강화, 연구지원을 위한 새로운 모형 개발, 모기관의 특화된 주제분야 지원, 참여 확대와 공공단체 협력 측면에 중점을 두고 있다.

Visual appearance의 정량적 측정을 위한 양방향 반사도 분포 함수 기반의 Coarseness 예측 방법 개발

한완희(Wanhee Han),서수환(SooHwan Seo),임진상(Jinsang Lim),박노철(No-cheol Park) 한국생산제조학회 2021 한국생산제조시스템학회 학술발표대회 논문집 Vol.2021 No.7