마늘의 callus 와 재분화 식물체의 핵형적 변이

서봉보,김윤경 한국유전학회 1991 Genes & Genomics Vol.13 No.2

The karyotypes in callus cells and regenerated plants derived from the callus of Allium sativum L. were analyzed. The leaflet discs of A. sativum proliferated into sufficient callus for subculturing after 120 days of initial culture. Karyotypical analysis was done every 30 day interval. Among the various ploid levels observed in the callus, diploid cells were most frequent and other ploid cells appeared in more or less low frequencies. The regenerated plants were diploid, tetraploid or mixoploid. The mixoploid showed a tendency to be stabilized as diploid or tetraploid as they grow.

파의 RAPD 산물의 클로닝 및 다색 FISH 를 이용한 물리학적 유전자 지도작성

서봉보 경북대학교 생명공학연구소 1998 遺傳工學硏究所報 Vol.13 No.1

N/A

다색 FISH 를 이용한 Allium 식물의 물리학적 유전자 지도 작성

서봉보 경북대학교 생명공학연구소 1997 遺傳工學硏究所報 Vol.12 No.1

N/A

쪽파의 임성회복을 위한 원행질체 배양과 파와의 종간잡종에 의한 식물체 재분화

서봉보,고종민,Nair, A . S . 경북대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.7 No.1

Leaf mesophyll protoplasts isolated from A. wakegi were fused with protoplasts derived from young leaf sheath bases of A. fistulosum. The somatic hybrid cells were cultured in 8p-KM medium (4M glucose, 0.1M mannitol, 0.4 ㎎/ℓ 2,4-D, 2 ㎎/ℓ NAA and 1 ㎎/ℓ BAP). During subsequent addition of fresh medium, the nitrogen source was replaced with 1 gm/ℓ glutamine and NAA was reduced to 1 ㎎/ℓ and 0.1 ㎎/ℓ respectively, After 11 days, MS medium(30 gm/ℓ sucrose, 0.1 ㎎/ℓ NAA and 1 ㎎/ℓ BAP) was added in three days intervel upto 40-60 days till micro calli (3㎜) were obtained.

쪽파와 파의 융합세포 배양에 의한 캘러스 유도

서봉보,고종민 한국유전학회 1993 Genes & Genomics Vol.15 No.4

Leaf mesophyll protoplasts isolated from amphidiploid Allium wakegi were fused with protoplasts derived from young leaf sheath bases of A. fistulosum. The somatic hybrid cell was cultured in KM8P medium supplemented with 0.4M glucose, 0.1M mannitol, 0.4㎎/ℓ 2,4-D, 2㎎/ℓ NAA and 1㎎/ℓ BAP for 5days. Then the medium was diluted by adding fresh KM8P medium with 1g/ℓ glutamine (substituted for ammonium nitrate and potasium nitrate), 0.4M glucose, 1㎎/ℓ NAA and 1㎎/ℓ BAP. After three days the medium was diluted with KM8P medium with 1g/ℓ glutamine,0.3M glucose, 0.1㎎/ℓ NAA and 1㎎/ℓ BAP. When MS medium with 30g/ℓ sucrose, 0.1㎎/ℓ NAA, and 1㎎/ℓ BAP was added into the petri dishes in three days intervals, and callus was obtained.

마늘의 이상 이배체와 사배체 재분화체의 핵형적 분석

서봉보,김윤경 한국유전학회 1991 Genes & Genomics Vol.13 No.4

Organogenetic ability in callus culture and the karyotypes in regenerated plants derived from the callus of Allium sativum L. were analyzed. Organogenetic ability in callus culture depended on the duration of culture on the medium with 2, 4-D and organogenesis-inducing medium (IAA+Kinetin). 347 regenerants out of total 467 were analyzed for chromosome numbers. They consisted of diploid, tetraploid, aneuploid and mixoploid as a frequency of 287 (0.83), 51 (0.15), 5 (0.01) and 4 (0.01), respectively. Karyotypic analyses were carried out in 169 plantlets whose chromosome numbers checked. Normal diploid, abnormal diploid, normal tetraploid, abnormal tetraploid, aneuploid and mixoploid were found to be 113 (0.67), 29 (0.17), 15 (0.09), 4 (0.02), 4 (0.02) and 4 (0.02), respectively. Chromosomal variations in number and structure were observed in abnormal diploid and tetraploid. Chromosome additions were mainly found in a, b, c, d, a and h chromosome but deletions in f and g chromosome. Elongated a chromosome, different form of h chromosome and very small chromosome were also observed in regenerated abnormal diploid. Deleted short arm in d chromosome were found in abnormal tetraploid. Short arm deletions of satellite chromosome were observed in abnormal diploid and tetraploid regenerants.

문주란과 아프리카문주란의 핵형분석

徐奉甫,李慶熙,金允景 慶北大學校 1992 論文集 Vol.52 No.-

The karyotypic analyses of Crinum asiaticum var. japonicum and C moorei var. album were carried out using conventional staining and Giemsa C-banding techniques, and distribution of B-chromosome was investigated. Chromosome compositions were diploid of 2n=22 in both species, of which five pairs including sat-chromosomes were submetacentrics, five pairs metacentrics and one pair subtelocentrics in C. asiaticum var. japonicum, but five pairs including sat-chromosomes were submetacentrics and the rest six pairs metacentrics in C. moorei var. album. Chromosome length was in a range of 5.6±0.4μm and 17.5±1.4 ㎛ in C. asiaticum var. japonicum, and 6.7±0.6㎛ and 15.8±4.7㎛ in C. moorei var. album. Consequently a little more variation in chromosome length was observed in C. asiaticum var. japonicum. Giemsa C-banding distribution of two species was very low, and thick C-bands were observed only near secondary constriction part of chromosome g. But there was a little difference in appearence of minor C-bands between two species, and this considered to be a characteristic of both species. B-chromosomes were observed only in C. asiaticum in a number of one to seven. But distinguishable morphological differences according to the different B-chromosome number were not observed.

한국인의 지(趾)및 지(指)발모절수(發毛節數)에 관한 유전

서봉보 한국유전학회 1980 Genes & Genomics Vol.2 No.1

The mode of inheritance of the number of segments with toe and digital hair in Korean populations was analysed in a sample of 2585 individuals comprising of 1235 male, and 1350 females including 648 sons and 720 daughters in 422 families. The mean of the number of segments with toe hair for males was 4.18 ± 1.62, while that for females was 3.97 ± 1.67. The difference between these means was significant. The frequency distribution was somewhat negatively skewed in both sexes. The parent-child correlation coefficient was 0.45 ± 0.02 and that for the midparent-child was 0.65 ± 0.02, while the interparental correlation was 0.03 ± 0.05. The sib-sib correlation was calculated to be 0.46 ± 0.03 for a sample of 395 pairs. There was neither any indication of X-linked nor Y-linked influence from the combination of father-son, father-daughter, mother-son and mother-daughter, as well as no maternal effect from the test between father-child and mother-child correlation. The regression of child on midparent was 0.80 ± 0.03, and a linearity test did not indicate any sign of dominance, although the dominant variance was to be 0.04. The heritability was measured to be about 0.80-0.94. Therefore, it may be conclude that this character is controlled by a polygenic system with almost complete additive effects. In the case of digital hair, the results showed that the parent-child correlation was 0.41 ± 0.02 and that for the midparent-child was 0.59 ± 0.02, while the interparental correlation was 0.01 ± 0.05. The sib-sib correlation was found to be 0.43 ± 0.04 from variance analysis for sibships. Also there was neither any indication of sex-linked inheritance nor of maternal effects. The regression of child on mid-parent was 0.71 ± 0.03. A linearity test did not reveal any sign of dominance, but dominant variance was calculated to be 0.09. The heritability for digital hair was lower than that of the toe hair, appearing to be 0.7-0.9. Thus, the mode of inheritance of digital hair was found to be similar to that of the toe hair.

닭 ( Gallus domesticus ) 의 경제형질 개량을 위한 염색체 분염분석과 표지인자의 구명에 관한 연구 3 : 교잡종과 순종간의 비교

서봉보,오봉국,여정수 한국육종학회 1985 한국육종학회지 Vol.17 No.1

Allium fistulosum 과 Allium ascalonicum 에서 헤테로크로마틴 분포에 의한 핵형분석

서봉보(Bong Bo Seo),김종호(Jong Ho Kim) 한국식물학회 1975 Journal of Plant Biology Vol.18 No.3

The present study demonstrates karyotype based on H-patterns of A.fistulosum and A. ascalonicum using Giemsa technique. The results obtained in this study are summarized as follows: ⅰ). Karyotypic analysis of A.fistulosum is 6VⅡ+JⅡ^t+JⅡ and that of .A. ascalonicum collected from a local farm in the suberbs of Taegu city clearly heterozygous as 13V+J_1^t+J_2+ⅰ. ⅱ). The heterochromatin of both species is generally located distally in both arms of chromosomes and each chromosome type possesses some variations on H-patterns. ⅲ). The percentage of heterochromatin to total chromosome length in cell is about 14.6% in A. fistulosum, 12.8% in A. ascalonicum. The number of bands is revealed about 38 in A. fistulosum and 33 in A. ascalonicum. Also in the amounts of chromocenters per nucleus, the former is somewhat more than the latter.