인삼에서 추출한 글리코시드가 배양한 마우스 섬유아세포의 핵산합성에 미치는 영향 ( Antiproliferative Effects of Panax ginseng Glycosides on DNA Synthesis in Cultured Mouse Fibroblasts )

변부형,신인철,이일하,조철오 고려인삼학회 1995 Journal of Ginseng Research Vol.19 No.2

Lard 와 Alcohol 을 섭취시킨 흰쥐 혈청중의 효소활성에 인삼추출물이 미치는 영향

변부형,김석환 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.3

The present study has been undertaken to investigate the effects of ginseng extract on the activities of several enzymes in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130 g were divided into 7 groups, each group receiving a different diet for 10 weeks; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 5% ginseng extract, basal diet plus 15% lard and 5% ginseng extract. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, and AST, ALT, lactate dehydrogenase, alkaline phosphatase activities in serum of rats. The results obtained were as follows: Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but ginseng extract caused an increase in food efficiency ratio. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but ginseng extract feeding decreased enzyme activities compared to lard and alcohol containing group. The above results suggest that ginseng extract would prevent the metabolic disease of liver by preventing hyperlipemia caused by high fat diet.

인삼추출물이 포화지방산 Lard 와 Alcohol 을 먹인 흰쥐 혈청 지질성분에 미치는 영향

변부형 한국식품위생안전성학회 1999 한국식품위생안전성학회지 Vol.14 No.2

The present study has been undertaken to investigate the effects of crude ginseng extract added to food on the lipid composition in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130 g were divided into 7 groups, each group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5% crude ginseng extract, basal diet plus 15% lard and 0.5% crude ginseng extract. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, triglyceride, total cholesterol, HDL-cholesterol, free cholesterol. The results obtained were as follows: 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but crude ginseng extract caused an increase in food efficiency ratio. 2. Lard supplementation group showed increase in the weight of liver, kidney, spleen, but another groups did not. 3. The contents ratio of triglyceride, total cholesterol in serum of lard-fed group showed significant increase compared to controls and but the simultaneous supplementation of crude ginseng extract and lard decreased sinificantly than those of lard-fed group. 4. The contents of free-cholesterol and ester cholesterol in serum of the simultaneous supplementation of crude ginseng extract group decreased as compared with the lard single fed group. 5. Crude ginseng extract single-fed group showed increse of plasma HDL-cholesterol compared to the control but not in any other groups. 6. The ratio of contents of VLDL, LDL-cholesterol and HDL-cholesterol in serum was significantly high in the only lard containing group and alcohol group, the crude ginseng extract supplemented group was showed lower tendency than only lard and alcohol group. The above results suggest that crude ginseng extract would prevent the metabolic disease by improving hyperlipidemia.

Lard와 Alcohol을 攝取한 흰쥐血淸中의 脂質成分에 마늘이 미치는 影響

변부형 慶山大學校 1995 論文集 Vol.13 No.1

The present study has been undertaken to investigate the effects of garlic added to food on the lipid composition in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130g were divided into 7 groups, each group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5% garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, triglyceride, total cholesterol, HDL-cholesterol, free cholesterol. The results obtained were as follows : 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. 2. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. 3. The contents ratio of triglyceride, total cholesterol in serum of lard-fed group showed significantly increase compared to controls and but the simultaneous Supple-mentation of garlic and lard decreased significantly than those of lard-fed group. 4. The contents of free-cholesterol and ester cholesterol in serum of the simultaneous supplementation of garlic decreased as compared with the lard single fed group. 5. Garlic single-fed group increased plasma HDL-cholesterol compared to controls but in any other groups were not changed. 6. The ratio of contents of VLDL, LDL-cholesterol and HDL-cholesterol in serum was significantly high in the only lard containing group and alcohol group, the garlic supplemented group was showed lower tendency than only lard and alcohol group. The above results suggest that garlic would prevent the metabolic disease by improv-ing hyperlipemia.

Effect of Platycodi Radix powder on Enzyme activities of Obese Rats Serum Fed High Fat Diet

변부형,서부일 대한본초학회 2003 大韓本草學會誌 Vol.18 No.4

마늘成分이 Lard와 Alcohol을 攝取한 흰쥐 血淸中의 酵素活性에 미치는 影響

변부형 慶山大學校 1994 論文集 Vol.12 No.1

The present study has been undertaken to investigate the effects of garlic added to food on the activities of several enzymes in serum of rats lard and alcohol. Thirty-five males of Sprague-Dawley stains weighed about 130 g were divided into 7groups, earth group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5 % garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, and glutamic oxa1acetic transaminase, glutamic pyruvic transaminase, lactate dehydrogenase, alkaline phogphatase activities in serum of rats. The results obtained were as follows : 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. 2. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. 3. AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but garlic feeding decreased enzyme activities compared to lard and alcohol containing group.

Stearic acid, Oleic acid, Elaidic acid에 의한 간세포의 성장 및 지방산 조성의 변화

변부형 慶山大學校 1997 論文集 Vol.15 No.1

This study was carried out in order to investigate the effect of stearic acid (18:0,SA), oleic (18:1cis.0A) and elaidic acid (18:1 trans, EA) on the cell growth and fatty acid composition of Hep-G2 cells. The cells were incubated in serum-free medium 25, 50, 100 and 200uM of fatty acid combined with albumin for 2 days. The fatty acid concentration up to lOOuM showed the normal growth, but the cell growth decreased in the presence of 200uM fatty acid. The treatment of cells with lOOuM of a fatty acid for two days significantly (p<0.05) increased the cellular triglyceride (TG) content in all fatty acid groups compared to control, but TG content was not significantly different among all treatment group, but total cholesterol (TC) was the highest level in EA group. Cellular phospholipid(PL) contents were similar between the control and all faty acid groups.

Repression of Transcriptional Activity of Estrogen Receptor alpha by a Cullin3/SPOP Ubiquitin E3 Ligase Complex

변부형,Yunhwa Jung 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.2

The role of SPOP in the ubiquitination of ERα by the Cullin3-based E3 ubiquitin ligase complex was investigated. We showed that the N-terminal region of SPOP containing the MATH domain interacts with the AF-2 domain of ERα in cultured human embryonic 293 cells. SPOP was required for coimmunoprecipitation of ERα with Cullin3. This is the first report of the essential role of SPOP in ERα ubiquitination by the Cullin3- based E3 ubiquitin ligase complex. We also demonstrated repression of the transactiva

전립선 비대증의 동서의학적 고찰

변부형,서부일,신상기 대구한의대학교 제한동의학술원 1999 제한동의학술원논문집 Vol.4 No.1

마늘성분이 Lard 와 Alcohol 을 섭취한 흰쥐 혈청중의 효소활성에 미치는 영향

변부형,김석환,정혜광,남철현,노병의 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.3

The present study has been undertaken to investigate the effects of garlic added to food on the activities of several enzymes in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130g were divided into 7 groups, each group receiving a different diet for 10 weeks; i.e. basal diet plus 15% lard, basal diet plus 5010 alcohol, basal diet plus 0.5% garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, and AST, ALT, lactate dehydrogenase, alkaline phoaphatase activities in serum of rata. The results obtained were as follows: Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but garlic feeding decreased enzyme activities compared to lard and alcohol containing group. The above results suggest that garlic would prevent the metabolic disease of liver by improving hyperlipemia caused by high fat diet.