Dactinomycin이 임신말기 흰쥐 자궁근충의 Adenosine Triphosphatase 활성에 미치는영향

백태경,정호삼,이규식 대한해부학회 1986 Anatomy & Cell Biology Vol.19 No.2

Dichlorvos가 흰쥐 심근의 미세구조에 미치는 영향

백태경,이화모,정호삼,Baik, Tai-Kyoung,Lee, Wha-Mo,Chung, Ho-Sam 한국현미경학회 1994 Applied microscopy Vol.24 No.3

It is well known that dichlorvos (DDVP), an organophosphate insecticide in common use, is so easily and rapidly hydrolyzed and excreted that it has usually little toxic effect on human body. In these days, however, it is widely used as an industrial and domestic insecticide and as an anthelmintic agent for animals, so that the accident of chemical poisoning occurs frequently. DDVP acts as a powerful inhibitor of carboxylic esterase, which can cause accumulation of acetylcholine at the synapses so paralysis of muscle and the transmission failure in cholinergic synapses dueing to desensitization of acetylcholin receptor may occure. Moreover accumulation of the acetylcholine brings about the elevation of the cyclic-AMP, which alters the cellular metabolisms of nucleic acid, carbohydrate, protein and lipid. Present study has undertaken to investigate the cardiotoxic effect of DDVP by electron microscopic study. A total of 30 Sprague-Dawley strain rats, weighing about 250gm were used as experimental animals. 2mg/kg/day of DDVP is intraperitonealy injected 3 times with intervals of every other day. On 1 day, 3 days, 5 days, 7 days and 14 days after drug administration, the animals were sacrified by cervical dislocation. Left ventricular cardiac muscles were resected and sliced into $1mm^3$. The specimens were embedded with Epon 812 and prepared by routine methods for electron microscopical observation. All preparations were stained with lead citrate and uranyl acetate and then observed with Hitachi-600 transmission electron microscope. The results were as follows: 1. In the cardiac muscle of DDVP treated rats, mitochondria with disorganized double membrane and mitochondrial crista, and vacuole formation in mitochondrial matrix were observed. But structures of mitochondria were recovered to normal in 14 days group. 2. In the cardiac muscle of DDVP treated rats, cisternae of sarcoplasmic reticulum were dilated and sacculated. But these changes were recovered to normal in 14 days group. 3. In the cardiac muscle of DDVP treated rats, glycogen particles around damaged myofibrils were decreased. But amount of glycogen particles were restored in 14 days group. 4. In the cardiac muscle of DDVP treated rats, disruption and discontinuation of myofilaments and disorganization of Z-disc were observed. But the structures of myofibrils were recovered to normal in 14 days group. It is consequently suggested that DDVP would induce the reversible degenerative changes on the ultrastructures in cardiac muscle of rat.

cis-Dichlorodiammineplatinum (II) 이 생쥐 폐포간중격의 미세구조에 미치는 영향

백태경,권익승,김원규,백두진,정호삼,이규식,Baik, Tai-Kyeoung,Kwon, Ik-Seung,Kim, Won-Kyu,Baik, Doo-Jin,Chung, Ho-Sam,Lee, Kyu-Sik 한국현미경학회 1993 Applied microscopy Vol.23 No.1

cis-Dichlorodiammineplatinum (II) (cis-Platin), a metallic compound, has widely been used as an effective anticancer chemotherapeutic agent. The precise mechanism of action of this agent is still unknown, but it is postulated that cis-Platin may act on the cancer cell like bifunctional alkylating agents. Although this agent is very beneficial to the patients with cervical cancer, germinoma of testis, neuroblastoma and others, it may also damage to the normal cell so that many side effects; severe hemorrhagic enterocolitis, bone marrow depression, renal damage and liver damage will develope. This experiment has been undertaken to pursue the cytotoxic effects of the cis-Platin on the ultrastructures of the interalveolar septum in the mouse lung. A total of 55 healthy male mice of ICR strain were used as experimental animals and divided into 5 mice of normal control group and 50 mice of cis-Platin treated group. The mice of cis-Platin treated group were sacrificed by carotid exsanguination at 6, 12, 24 hours, 3 days and 7 days after intraperitoneal injection of 6.0 mg of cis-Platin ($Abiplatin^R$ Abic Co. Ltd.) per kg of mouse body weight. The specimen obtained from the lower lobe of left lung were sliced into $1mm^3$ and prefixed with 2% glutaraldehyde -2.5% paraformaldehyde solution prepared with Millonig's phosphatae buffer solution (pH 7.4) at $4^{\circ}C$ for 3-4 hours. After postfixation with 1% osmium tetroxide solution all specimens were embedded in Epon 812. Ultrathin sections about $600-800{\AA}$ in thickness were stained with uranyl acetate and lead citrate and observed with Hitachi-600 electron microscope. The results obtained were as follows: 1. Local swellings with increase of electron density and number of pinocytic vesicles in the cytoplasms of the type I pneumocyte and endothelial cell of the blood air barrier in interalveolar septum of cis-platin treated mice were observed. 2. Cisternae of rough endoplasmic reticulum were dilated and sacculated in association with detachment of membrane bound ribosomes of the type II pneumocyte in interalveolar septum of cis-Platin treated mice. 3. Swollon mitochondria with uneven electron density of their matrix were observed in the type II pneumocyte of interalveolar septum in the cis-Platin treated mice. 4. The lamellae of lammelar bodies in type II pneumocyte of interalveolar septum in cis-Platin treated mice were devoided or transformed into homogeneous electron dense material. It is consequently suggested that cis-Platin would induce the cellular edema of type I pneumocyte and endothelial cell, and degenerative changes of cytoplasmic organelles of the type II pneumocyte in the interalveolar septum of the mouse lung.

지적도와 건축물대장 연계를 통한 토지이용 DB구축에 관한 연구

백태경,김영훈,최정미 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.4

도시토지이용 문제는 인구와 시설이 증가함에 따라 점점 심각해지고 있으며 토지이용계획은 도시계획에서 가장 중요한 부분이다. 따라서 도시토지이용의 현상을 파악하고 미래를 예측하는 것은 토지이용계획의 핵심적인 과정이라 할 수 있다. 본 연구에서는 지방자치단체의 도시계획업무에 사용되는 토지 및 건축물대장의 전산자료를 활용하여 도시해석 시 사용가능토록 DB를 구축하여 활용방안을 검토하였고, 이미 개별 목적으로 작성되어 있는 지적도와 건축물대장의 연계를 통한 개별 필지별 토지이용 데이터를 구축하여 그 활용방안을 검토하고, 토지이응현황 등을 분석하였다. 이러한 정보체계를 이용하여 공간정보와 그 속에 존재하는 토지이용속성을 시계열적 공간자료로 활용하여 부산시 토지이용 현황을 파악하였다. As the city grows in population and size, land-use plan increasingly becomes a crucial element in developing a city-wide plan. In the process of developing the land use plan, identifying the present conditions and predicting the future conditions are the most important steps. That is, to understand the phenomena of land-use activities as well as to manage uses of the land efficiently require the comprehensive land use information system. This study intends to construct the land use database utilizing the Cadastral Map and Registered Building Data and to examine and suggest the application areas of the database. The database was constructed with those of Busan Metropolitan City. Using the established data, it shows how to use the database to identify the present conditions in Busan.

한국지리정보학회지 논문 투고 현황에 관한 분석 - 최근 7년(2001년~2007년)을 중심(中心)으로 -

백태경,Baek, Tae-Kyung 한국지리정보학회 2008 한국지리정보학회지 Vol.11 No.1

1998년부터 발간이 시작된 한국지리정보학회(KAGIS)의 한국지리정보학회지는 1998년 1권1호(통권1호)가 발간된 이래 2007년 12월 현재 10권4호(통권37호)까지 발간되었다. 그 중에서 최근 7년간(2001년~2007년)에 투고 되거나, 게재된 논문을 정리하여 그 특성을 분석하였다. 분석을 통하여 본 학회에 투고된 논문의 전공분야, 지역별 분포, 소속기관별 현황 등을 파악하였다. 자료의 정리를 통해 파악한 결과를 보면 투고자의 소속기관은 대학 및 연구소로 편중 되어 있었고, 직급 및 직위에 있어서도 대학교수와 연구원이 94%를 차지하고 있어 관련기업 종사자의 투고율이 낮은 것으로 나타났다. 앞으로의 학회지 발간에 있어 다양한 분야의 전공자들이 참여할 수 있도록 유도 할 필요가 있으며 다양한 분야의 심사자를 확보함으로써 한국학술진흥재단(KRF)의 A급 등재지로서의 위상을 확립하고 발전시키는 계기가 되었으면 한다. 아울러, 본 고에서 분석한 자료들은 우리나라 GIS정책수립 등에 있어서 유용한 참고자료로 활용되리라 기대된다. The purpose of this study is analyze to characteristics and research trend of geographic information system as reviewing research trend of KAGIS (Korean Association of Geographic Information Studies) journals published from 2001 to 2007. Also, 358 articles have been published and characteristic of a contributor attribute and research categories were analyzed. Professor and the research 94% hold and the contribution ratio of the relation enterprise worker appeared with the low. The results of this study will be useful for guidelines to establish NGIS and strategies.

수치지도를 활용한 주제도 작성에 관한 연구

백태경,신용은 한국지리정보학회 2003 한국지리정보학회지 Vol.6 No.4

본 연구는 2003년부터 ‘도시계획법’과 ‘국토이용관리법’이 ‘국토의 계획 및 이용에 관한 법률’로 통합 제정되면서 ‘도시계획정보체계’가 ‘국토이용정보체계’로 전환됨에 따라 국토이용정보체계에서 활용할 수 있는 의사결정 지원을 위한 지표 개발과 주제도 작성을 목적으로 한다. 지표 개발을 위한 기초적 작업으로서, 부산광역시 각 읍?면?동의 사회?경제데이터와 수치지도상의 공공 편익시설 레이어를 이용하여 데이터베이스를 구축하였다. 구축된 데이터베이스를 이용하여 첫째, 다변량해석(multivariate analysis)을 통한 부산광역시 공간구조분석을 실시하고 이를 지도로 표현하여 하나의 주제도를 작성하였으며 둘째, 수치지도의 공공 편익시설 레이어와 읍?면?동과의 최소거리를 측정하여 각 시설별 주제도를 작성하였다. In early year 2003, the previous Urban Planning Law and National Land Use Management Law were abolished and integrated into the Law regarding National Land Planning and Land Use Management. Accordingly, previous Urban Planning Information System is reformed into National Land Use Information System. This paper intends to develop indexes supporting decision-making process and to construct theme map using digital map that can be utilized for the National Land Use Information System. As a base for the index development, necessary database(DB) was established by utilizing the socio-economic information of towns and villages in Busan and a layer of public facilities. Base on the DB, multivariate analyses were performed to examine and analyze the spatial structure of Busan. It was then expressed constructed as a theme map. The facility theme map was also constructed based on a layer of public facilities and the shortest distance between towns and villages.

GIS DB를 이용한 상업,업무시설의 입지 포텐셜 분석

백태경,최정미 한국지리정보학회 2006 한국지리정보학회지 Vol.9 No.1

연령에 따른 백서 진피내 기질의 성상변화에 관한 형태학적 연구

정호삼,김진,홍성희,백태경 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.1

To investigate the chronological changes of fiber components and glycosaminoglycan composition, the skins of medial thigh of fetal rats of the 21th gestational day, and rats of 3rd day, lst, 3rd, 7th, 15th and 40th weeks of postnatal life are resected and then fixed with 10% neutral buffered formalin at 4℃ for 24 hours. According to routine procedures for parraffin section, preparations with 5㎛ thickness were male Hematoxylin-eosin stain for observing the histological changes, Verhoff-Van Gieson stain for collagen and elastic fibers and alcian blue(pH 2.5)PAS stain for mucopolysaccharides were done. All preparations were observed with light microscope. The results were as follows : 1. The papillary layer and reticular layer of rat dermis are distinguished from the 3rd day of postnatal life. Vasculare is most abundant in the 7th week but after the 15th week, number of blood vessels is gradually reduced. 2. The collagen fibers in rat dermis are compacted in papillary layer on the 3rd day of postnatal life. From the lst week of postnatal life, polymerized thick collagen fibers appear in the papillary layer. At the 40th week, amount of collagen fibers is reduced. 3. The elastic fibers in rat dermis appear at the lst week of postnatal life. Well developed thick elastic fibers are observed in papillary layers at the 7th and 15th weeks. Fragmented and deformed elastic fibers are seen in the reticular layer at the 15th and 40th weeks. 4. On the 21st day of gestation, weakly positive reaction of for alcian blue is observed in the dermis. The alcianophility is gradually decreased until the lst week of posnatal life. On the 3rd week, moderately positive reaction for alcian blue is seen in the dermis. After the 7th week alcianophility is reduced. Itis consequently suggested that maturation of collagen and elastic fiber is achieved at the 7th postnatal week, and synthesis and secretion of mucopolysaccharides are most active at the 3rd postnatal week in albino rat.

Vincristine Sulfate가 백서 척수내 신경원의 Acetylcholinesterase 활성에 미치는 영향

이규식,이군자,정호삼,김승현,백태경 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

Vincristine은 협죽도과에 속하는 열대식물인 Madagascum periwinkle에서 추출한 천연 alkaloid계 항암제로서 암세포의 미소관 형성을 억제하고 미소관을 파괴하며 또한 핵산 및 단백질합성을 방해하여 암세포의 증식을 억제하므로 현재 백혈병 및 임파종등 혈액종양과 여러 충실성종양의 치료에 유용하게 사용된다. 그러나 다른 항암제와 마찬가지로 암세포에만 특이하게 작용하지 않고 정상세포에도 손상을 야기하므로 vincristinc을 투여받은 암환자에서 혈액독작용, 소화기장애, 피부장애 및 신경독작용등 여러 부작용이 유발되고 특히 신경독 작용은 vincristine을 사용을 제한하는 가장 심각한 장애로 알려져있다. 저자는 백서에 vincristine을 투여하면 vincristine이 실험동물의 척수내 신경원에 손상을 야기하여 척수내 신경원의 acetylcholinesterase활성에 변화가 초래될 것으로 사료되어 본 실험을 시도하였다. 실험동물로는 체중 200gm내외의 Wistar계의 웅성 백서 35마리를 사용하여 백서 체중 kg당 0.5mg의 vincristine sulfate(Abic Ltd.) 0.2cc를 1일 1회씩 5일간 복강내로 주사하였고 정상대조군 백서에는 동량의 생리적 식염수를 같은 방법으로 투여하였다. 실험동물은 최종 주사후 24시간 및 72시간 경과 후에 후두부 강타로 도살하고 10% neutral buffered formaline으로 관류시킨 후 육안으로 해부하여 척수를 절취하고 10% neutral buffered formaline에 4℃에서 18시간 고정후 일부 조직은 paraffin에 포매하여 Nissl체를 관찰하기 위한 cresyl violet염색을 시행하고, 일부조직은 -20℃로 조절된 cryostat로 두께 10㎛의 동결절편을 제작하여 Gredtzoff(1959)의 방법에 따라 acetyl-cholinesterase활성을 관찰하기 위한 조직화학적 처리를 한 후 광학현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. Vincristine투여군 백서의 척수내 체성운동신경원 및 내장성운동신경원에 chromatolysis가 유발되었으며, 소신경원의 수와 cresyl violet 에 대한 염색성이 감소되었다. 2. Vincristine투여군 백서 척수경부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성 운동신경원에서는 강한 양성반응을 나타내었고 배측각에서는 약한 양성반응을 나타내었으며 중간부에서는 미약한 양성반응을 나타내었으나 3일 경과군의 복측각내 체성운동신경원과 배측각내 교양질부에서는 중등도 양성반응을 나타내었으며 중간부에서는 미약한 양성반응을 나타내었다. 3. Vincristine투여군 백서 척수흉부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성운동신경원과 외측각내 내장성운동신경원에서는 약한 혹은 중등도 양성반응을 나타내었고 배측각에서는 약한 양성반응을 나타내었으며 척수수질의 중간부에서는 미약한 양성반응을 나타내었으나, 3일 경과군의 복측각내 체성운동신경원에서는 중등도 양성반응을 나타내었고 외측각내 내장성 운동신경원에서는 강한 양성반응을 나타내었으며 배측각내 교양질부에서는 중등도 양성반응을 나타내었으나 배측각의 나머지 부분에서는 약한 양성반응을 나타내었고 척수수질의 중간부에서는 미약한 혹은 약한 양성반응을 나타냈었다. 4. Vincristine 투여군 백서 척수요부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성운동신경원에서는 중등도 혹은 강한 양성반응을 나타내었고 배측각의 교양질부와 Lamina Ⅳ에서는 중등도 양성반응을 나타내었으며 배측각의 나머지 부분은 약한 양성반응을 나타내었고 척수수질의 중간부에서는 미약한 양성반응을 나타내었으나, 3일경과군의 복측각내 체성운동 신경원에서는 약한 혹은 중등도 양성반응을 나타내었고 배측각내 교양빌부와 Lamina Ⅳ에서는 약한 양성반응을 나타내었으며 척수수질의 중간부에서는 미약한 혹은 약한 양성반응을 나타내었다. 이상의 소견을 종합한 바, vincristine은 백서 척수내 신경원에 세포독으로 작용하여 신경원 손상을 초래하고 신경원의 acetylchlinesterase활성을 억제 하나, 이러한 손상은 시간이 경과함에 따라 일부 회복되는 것으로 사료된다. Vincristine, one vinca alkaloids, is widely used for chemotherapy of a variety of malignancy. The mechanism of anticancerous activity of this agent is based on inhibition of mitosis by interference with development of microtuble, of syntheses of nucleic acid and protein, and of destruction of cytoplasic membrane and microtubule. However, these inhibitory effects on cancer cell proliferation are non-specific, so clinical use of vincristine is frequently limited due to its toxic effects on the hematopoietic and nervous system especially. The author had undertaken this study to pursue the effect of vincristine on the nuerons in rat spinal cord histolgically and histochemically. Totals of 35 healthy male Wistar stain albino rats, weighing about 200 gm, were used as experimental animals. The experimental animals were divided into normal control and vincristine treated groups, and then vincristine treated rats were subdivided into 24 hours and 72hours groups by time passage. All vincristine treated rats were abministrated intraperitoreally with 0.5mg of vincristine per kg of rat body weight diluted in 0.2cc of normal saline for 5 days and rats of vincristine treated group were sacrificed by occipital blow at 24 hours and 72 hours after administration and then perfused by 10% neutral buffered formaline. The spedimen, spinal cord was collected and them fixed with 10% neutral buffered formaline during 18 hours at 4℃. For observing the histological change, cresyl violet stain was performed by routine pareffin method, and for histochemical study of acetylcholinesterase, frozen sedtions of 10 ㎛ in thickness were treated with Gerebtwoff method(1959). The results obtained were as follows : 1. In vincristine treated rat, a part of the somatic and visceral motor neurons were brought on chromatolysis. And the numer and the stain activities of small neurons were decreased in cresyl violet stain. 2. The activities of acetylcholinesterase of cervical segment in vincristine treated rat spinal cord were strongly positive at somatic motor meuron, weakly positive at dorsal horn and tracely positive at intermediate zone in 1 day passed group after the final administration, and moderately positive at somatic motor neuron and substantia gelatinosa, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 3 days passed group. 3. The activities of acetylcholinesterase of thoracic segment in vincristine treated rat spinal cord weakly or moderately positive at somatic and visceral motor neurons, weakly positive at dorsal horn and tracely positive at intermdeiate zone in 1 day passed group after the final administration, and moderately positive at somatic motor mourons, strongly positive at visceral motor meurons, moderately positive in substantia gelatinosa, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 3 days passed group. 4. The activities of acetylcholinesterase of lumbar segment in vincristine treated rat spinal cord were moderately or strong positive at somatic motor meurons, weakly positive at substantia gelatinosa and lamina Ⅳ, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 1 day pssed group after the final administration, weakly or moderately positive at somatic motor neurons, weakly positive at sugstantia gelationsa and lamia Ⅳ and tracely or weakly positive at remainder of spinal medulla in 3 days passed group. It is consequently suggested that vincristine would induce the degeneration of neurons and inhibit acetylcholinesterase activity resulting from its neurotoxic effects on neurons in spinal cord of albino rats, but this damage would partially recover by time passage.

Vinblastine Sulfate가 Mouse간장의 인산염 분해효소 활성에 미치는 영향

백태경,박희철,정호삼 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Vinblastine sulfate isolated from the vinca has been widely used as an anticancer drug. Vinblastine sulfate inhibits the formation of microtubules which are related to cytoskeleton, transport of molecules and cellular polarity. As a result, it inhibits the mitosis and brings to cellular necrosis. The author undertook the present study to investigate the effect of binblastine sulfate on phosphatase activity in the mouse liver. Albino mice, DDY strain, weighing 2gm were used as experimental animals. The experimental animals were sacrificed at 6, 12, 24, 36 and 48 hours after administration of 12 mg/kg of of vinblastine sulfate. The activity of alkaline phosphatase was observed by Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein-Meisel's method for histochemical study. The results obtained were as follows: 1. The activity of alkaline phosphatase was slightly increased in the central zone and decreased in the intermediate and periportal zone of the hepatic lobule of the 24 and 36 hours vinblastine sulfate treated groups. In 48 hour-vinblastine sulfate treated group, the activity of alkaline phosphatase was recovered to normal. 2. The activity of adenosine triphosphatase was slightly increased in the hepatic lobule of the 12 and 24 hours vinblastine sulfate treated groups but decreased in the 36 and 48 hour vinbalstine sulfate treated groups. Consequently, it is suggested that vinblastine sulfate cause to decrease the activity of alkaline phosphatase and adenosine phosphatase in mouse liver.