확장된 개념에서의 가상성

백승혜(Seung-Hye Paek),김현주 한국HCI학회 2014 한국HCI학회 학술대회 Vol.2014 No.2

두피의 지루피부염에 대한 임상적 관찰과 Pityrosporum의 분포

백승혜,정상립,김도원,정현주,최성관 ( Seung Hye Paek,Sang Lip Chung,Do Won Kim,Hyeon Joo Jung,Sung Kwan Choi ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.1

Background: Seborrheic dermatitis is a very common chronic inflammatory disease. Previous studies suggested that, Pityrosporum, lipophilic fungi, may play an important role in the pathogenesis of the disease. Objective : The purpose of this study was to investigate the disease clinically and mycologically for better understanding of the pathogenesis. Methods : Two hundred and one new patient.s with seborrheic dermatitis of the scalp and 67 norrnal subjects were obs!,rved clinically by the dermatologist and studied stat,istically by questionnaires. A mycologic study of Pityrosporum was also performed by direct smear. Results : Among a total of 201 patients wit,h seborrheic dermatitis, 72.6% had pityriasis sicca, 10.5% had pityr iasis steartoides and 16.9% had inflammatory seborrheic dermatitis. On clinical grading by the Kligman.. scale, grade III(slightly scaling) was the most common in the patients and 52.6% of the norma. subjects were below grade II (no scaling). Spores of Pityrosporum were observed in 76.7% of the patients and 91.5% of the normal subjects. The incidence of Pityrosporum was lower particularly in t,he patients with inflammatory seborrheic dermatitis t,han in other pa~tients groups. Conclusion : According to the results of the study, it, is difficult to conclude that Pityrosporum is a main causative ager t of seborrheic dermatitis. In addition, it is thought t.hat further studies of the role of Pityrosporum in seborrheic dermatitis are needed. (Kor J Dermatol 1997;35(1): 22-32)

Nail Lacquer 가 Transonychial Water Loss에 미치는 영향

김도원,백승혜,이정우,정상립 ( Do Won Kim,Seung Hye Paek,Jeong Woo Lee,Sang Lip Chung ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.1

Background: Nail lacquer strengthens and protects the nail plate by preventing excess loss of moisture and providing a hard surface. On the other hand, it may produce allergic contact and irritant dermatitis, paronychial and nail bed inflammation and infection, dystrophic nails and onycholysis. Objective : The purpose of study was to investigate the effect of the nail lacquer on the barrier function of the nail. Methods : The study populatian consisted of 25 healthy young subjects who had not applied the nail lacquer to the nail for the last 3 months. Nail lacquer was applied to the left thumb and middle fingernail for 6 weeks and then removed by acetone. Transonychial water loss(TOWL) of the treated left fingernails and the untreated corresponding right fingernails was measured with an Evaporimeter. Lipids of the nails were extracted from distal nail cuttings and analysed by thin layer chromatography. Results . 1. Before application of the nail lacquer, there was no difference in TOWL between the left and right fingernails while the TOWL of the thumb nail was higher than that of the middle fingernail with a statistical significance(p<0.05). 2. The TOWL of the left fingernails, measured 1 day, 2, 4 and 6 weeks after application of the nail lacquer, was lower than that of the untreated right fingernails with a statistical significance( p< 0.05). 3. The TOWL of the left fingernails, measured 1, 3 and 7 days after removal of the nail lacquer by acetone, was not different statistically from that of the untreated right fingernails. 4. Lipid analysis demonstratcd that the cholesterol content was higher in the left fingernail which had been covered with nail lacquer than in the untreated right fingernail. 5. There was no clinical adverse effect observed during application of the nail lacquer or after removal of it. Conclusion : According to the results of the study, nail lacquer has some occlusive effects on transonychial water evaporatior . Because increased cholesterol content may be due to perturbations of barrier function , further studies of the effect of the nail lacquer on the barrier function of the nail will be needed. (Kor J Dermatol 1997 ; 35(1) : 82~89)

천규콘딜롬에서 발생한 피각

김용현 ( Yong Hyun Kim ),백승혜 ( Seung Hye Paek ),전재복 ( Jae Bok Jun ),정상립 ( Sang Lip Chung ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5

We report a case of cutaneous horn originated frorn condyloma acuminata in a 25 year-old man. The patient showed yellowish brown hyperkeratotic masses on the prepuce, coronal sulcus and multiple asymptomatic pin'kish cauliflower-like projections in both inguinal folds and perianal area. Skin biopsies were taken from the base of the penile lesions andinguinal, perianal area exhibited marked hyperkeratosis and parakeratosis in addition to the typical findings of condylorna acuminaturn. In situ hybridization technique for Human papilloma virus(HPV) showed positive reaction to HPV 11. All lesions were removed by surgical excision, electrocauterization and podophyllin application, but condyloma acuminatum recurred several times thereafter. (Kor J Dermatol 1996;34(5): 851-855)

향기 나는 장미 (원교 D1-409) 캘러스 추출물의 항산화 및 항염, 보습 효과

김수윤(Soo-Yun Kim),백승혜(Seung-Hye Paek),이수영(Su-Young Lee),송지혁(Ji-Hyeok Song),김지연(Ji Yeon Kim),이정훈(Jeong Hun Lee) 한국화장품미용학회 2022 한국화장품미용학회지 Vol.12 No.2

Since ancient times, roses were grown for many different reasons as cut flowers, garden plants, and pot plants as well as for industrial (perfume), medicinal, and culinary applications. Wongyo D1-409 was cultivated and bred in the National Institute of Horticultural and Herbal Science. The embryogenic callus derived from the roses root was induced by using plant tissue culture technology. Then, the callus was extracted with water for the efficacy test. In Analyzes using HPLC, various minor peaks were detected in the extracts. As a result, ellagic acid and gallic acid was identified as the characteristic peak. After extraction of callus extract, biological evaluation including anti-oxidant, anti-inflammation, and moisturizing effect was performed. As a result, the callus extract showed remarkable biological activities through stimulating synthesis of super oxide dismutase(SOD1), catalase(CAT), nuclear respiratory factor 1(NRF1) and aquaporin(AQP3) expression, and inhibiting cyclooxygenase-2(COX-2) expression. Therefore, it is expected that the domestic rose-derived embryonic callus extract can be used in various ways for anti-aging cosmetic materials.

김유래 미코스포린 유도체인 포피라334가 함유된 화장품 제형의 주름개선 효능 검증

장성주(Sung-Joo Jang),이삭(Sak Lee),백승혜(Seung-Hye Paek),신동선(Dong-Sun Shin),송지혁(Ji Hyeok Song),김재영(Jae-Young Kim),이상준(Sang-Jun Lee),모상현(Sang-Hyun Moh) 한국화장품미용학회 2021 한국화장품미용학회지 Vol.11 No.2

In this study, we focus on the Porphyra 334, one of mycosporine-like amino acids (MAAs). Porphyra 334 was purified from Porphyra yezoensis and its validation method was established. Anti-wrinkle activity of Porphyra 334 on the procollagen synthesis in Human dermal fibroblast (HDF) was evaluated that Porphyra 334 significantly increased synthesis of procollagen in HDF. The clinical test in vivo demonstrated that constant application of Porphyra 334 improved periorbital wrinkles compared with the placebo. In conclusion, Porphyra 334 might be a novel biomaterial for anti-wrinkle according to in vitro in HDF and clinical test in vivo.

한국 고유 식물 울릉도 돌외 식물 세포 배양추출물의 항염증 효과

목보람(Bo-Ram Mok),김수윤(Soo-Yun Kim),백승혜(Seung hye Paek),장영수(Young-su Jang),신정우(Jung U Shin),모상현(Sang Hyun Moh) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.2

이 논문의 목적은 울릉도 자생식물인 돌외식물(GP: Gynostemma pentaphyllum )을 이용하여 지속가능한 화장품 원료 개발을 위해 피부장벽개선 및 아토피피부염 개선 효능을 평가하고 검증하는 데 있다. 자연을 훼손하지 않으며 지속가능한 항노화 소재개발을 위하여 울릉도 자생식물인 돌외에서 식물세포를 유도하여 대량배양 조건을 확립, 대량배양된 식물세포로부터 다양한 용매로 추출 후, HPLC 분석을 통하여 adenosine, guanosine 및 tyrosine, phenylalanine 변화를 확인하였다. 또한, 돌외 식물세포의 피부장벽개선효능 및 항가려움증 효능평가를 위해 Th2 사이토카인을 이용한 in vitro 염증 모델에서 피부장벽관련 인자인 FLG, Zo-1의 유전자 발현에 유의미한 변화가 확인이 되지 않았지만 항가려움증 관련 인자인 TSLP, IL-33 의 유전자 발현에 유의미한 감소 변화를 확인하였다. 따라서 돌외식물세포 추출물이 가려움증을 개선시키는 데 유의미한 효능이 있을 것으로 확인되며, 나아가 돌외 식물세포 추출물이 지속가능한 자연친화적 활성 소재로써, 아토피피부염 개선을 위한 화장품에 널리 활용될 수 있을 것이라 사료된다. The purpose of this study was to evaluate and verify the effectiveness of sustainable cosmetic raw materials developed from Gynostemma pentaphyllum, a plant native to Ulleungdo, in improving the skin barrier function and treating atopic dermatitis. Cells were derived from adult Gynostemma pentaphyllum plants, and suitable conditions for mass culture of the cells were established in a bioreactor. DNA components and amino acids extracted from this mass culture were identified from the HPLC fraction. In the in vitro efficacy evaluation results, changes in the expression levels of skin barrier-related proteins such as filaggrin (FLG) and Zonula occludens-1 (Zo-1) were insignificant. It was confirmed that the expression levels of the proteins thymic stromal lymphopoietin (TSLP) and interleukin-33 (IL-33) were significantly reduced. These results lead to the conclusion that Gynostemma pentaphyllum cell extracts have significant anti-inflammatory effects and that these extracts can be widely used as sustainable, nature-friendly active material in cosmetics with anti-inflammatory effects and targeted at improving atopic dermatitis. They may find use in anti-aging cosmetic products as well.

Nail Lacquer가 Transonychial Water Loss에 미치는 영향

김도원,백승혜,이정우,정상립 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

Background : Nail lacquer strengthens and protects the nail plate by preventing excess loss of moisture' and providing a hard surface. On the other hand, it may produce allergic contact and ir-ritant dermatitis, paronychial and nail bed inflammation and infection, dystrophic nails and only-cholysis. Objective : The purpose of study was to investigate the effect of the nail lacquer on the barrier function of the nail. Methods : The study population consisted of 25 healthy young subjects who had not applied the nail lacquer to the nail for the last 3 months. Nail lacquer was applied to the left thumb and middle fingernail for 6 weeks and then removed by acetone. Transonychial water loss(TOWL) of the treated left fingernails and the untreated corresponding right fingernails was measured with an Evaporimeter. Lipids of the nails were extracted from distal nail cuttings and analysed by thin layer chromatography. Results : 1. Before application of the nail lacquer, there was no difference In TOWL between the left and right fingernails while the TOWL of the thumb nail was higher than that of the middle fin-gernail with a statistical significance(p<0.05). 2. The TOWL of the left fingernails, measured 1 day, 2, 4 and 6 weeks after application of the nail lacquer, was lower than that of the entreated right fingernails with a statistical signifi-cance(p<0.05). 3. The TOWL of the left fingernails, measured 1, 3 and 7 days after removal of the nail lac-quer by acetone, was not different statistically from that of the untreated right fingernails. 4. Lipid analysis demonstrated that the cholesterol content was higher in the left fingernail which had been covered with nail lacquer than in the untreated right fingernail. 5. There was no clinical adverse effect observed during application of the nail lacquer or after removal of it. Conclusion : According to the results of the study, nail lacquer has some occlusive effects on transonychial water evaporation. Because increased cholesterol content may be due to perturba-tions of barrier function, further studies of the effect of the nail lacquer on the barrier function of the nail will be needed. (Kor J Dermatol 1997;35(1):82∼89)