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신경세포가 별아교세포의 아교섬유성 산단백질 표현에 미치는 영향
배형미,박정선,연동수,Bae Hyung-Mi,Park Jung-Sun,Yeon Dong-Soo 대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.3
Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.
지속적 뇨 알칼리화 요법이 Gentamicin 신독증 예방에 미치는 영향
김영기,한대석,김홍수,이호영,하성규,손승환,최규헌,배형미 대한신장학회 1992 Kidney Research and Clinical Practice Vol.11 No.1
The nephrotoxicity of aminoglycoside such as gentamicin which is generally used in severe infections including gram-negative bacterial infection, has been a problem in clinical setting. To treat the infected patients with these agents aggressively, we need a method to prevent those nephrotoxicity. The study of continuous urinary alkalinization during the gentamicin therapy in Fischer 344 rats with regular renal function measurements suggested that,it could reduce the ne- phrotoxicity of gentamicin. To evaluate the preventive effects in human, we studied the effect of continuous urinary alkalinization on gentamicin nephrotoxicity in patients who had normal renal function and in whom gentamicin was indicated for their infections. We divided the patients into three groups randomly. The first group was given gentamicin (3 mg/kg/day) only (control group), the second group given gentamicin (3 mg/kg/day) and sodium bicarbonate (6 g/day), and the third group given gentamicin (3 mg/kg/day), sodium bicarbonate (6 g/day) and acetazolamide (1 g/day). BUN, serum creatinine, creatinine clearance, urine Ph, and urine beta-2-microglobulin (B,-MG)/creatinine ratio were measured before, during and after therapy. 1) Total 76 patients (14 males and 62 females) were studied. The mean age was 39.5?16.0 years in gorup 1, 35.7?14.4 in group 2, and 38.1?13.8 in group 3, which were not significantly different. The infections of those 76 patients were acute pyelonephritis in 50 cases, acute cystitis in 9 cases, pneumonia in 6 cases, gastroenteritis in 3 cases, salmonellosis in 2 cases, and others in 8 cases. 2) Urine Ph in each group before therapy were 6.19? 1.03 in group 1, 5.57?0.83 in group 2, 5,98?0.98 in group 3. Significant urinary alkalinizations were noted in group 2 and 3 in which the urinary Ph on the 7th and 14th day after gentamician therapy were 6.74?1.29 and 7.04?1.33 in group 2, and 6.88?1.33 and 6.93?1.27 in group 3, compared with 6.04?0.89 and 5.56?0.91 in group 1 (p$lt;0.05). 3) The increase in serum creatininan 20% of baseline value was noted in 10.l%(3/28 patients) of group 1, 4,5%(1/22 patients) of group 2, and 19.0%(4/21 patients) of group 3 on the 7th day of therapy, and 20.0%(2/10 patients), 20. 0%(3/15 patients) Blld 13.3%(2/15 patients) in each group on the 14th day. 4) The creatinine clearance (ml/min/1. 73 m') before, on the 7th and 14th day after therapy were 92. 33 ?39. 71, 98.79?33.54 and 102.49?24.11 in group 1, 99.05?37. 07, 93,84?27.37 and 94.13?30.78 in group 2, and 87. 62?34.82, 87.54?32.71 and 88.58?29.74 in group 3. And there was no significant difference. 5) The baseline ratio of B,-MG/creatinine in urine on the 3rd, 7th and 14th day after therapy were 1.61?2.62, 19.09?52.34 and 79.25?242.48 in gorup 1, 1.44?2.24, 11.86?30.98 and 39.78?137.7 in group 2, and 1.44?l. 23, 4.62?10.39 and 5.63?11.57 in group 3. The baseline ratio of B,-Mglcreatinine was decreased to 15.97?43. 10, 4.58?7.89 and 1.93?3.56 in each group on 3days after the end of the therapy. In conclusion, urinared the tendency of preventive effects on gentamicin nephrotox- icity in human, but follow up studies on the patients with high risks for the aminoglycoside nephrotoxicity would be needed to define more definite preventive effects of urinary alkalinization in these patients.
김영수,김흥수,강진경,박인서,전재윤,박찬일,홍천수,최흥재,배형미,양동규,정준표 대한소화기내시경학회 1991 Clinical Endoscopy Vol.11 No.2
Studies on hepatic tuberculosis are rare in Korea except several case repots. This is the first report on hepatic tuberculosis confirmed by the peritoneoscopic liver biopsy in Korea. A 43-year-old man was admitted due to high fever and cough for l0 days. On physical examination moist rale was audible on the both lower lung fields and hepatomegaly was noted. Chest X-ray revealed multiple fine granularity scattered uniformly throughout the both lung fields compatible with miliary pulmonary tuberculosis. On blood chemistry, SGOT, SGPT and alkaline phosphatase were elevated. Peritoneascopy revealed multiple yellowish-white small nodules evenly acattered on the entire surface of the both lobes of the liver and the needle biopsy of the liver showed chronic granulomatous inflammation with multinucleated giant cells and caseous necrosis consistent with hepatic tuberculosis. The patient was treated with antituberculous medications. Chest X-ray 6 months after treatment revealed completely healed miliary pulmonary tuberculosis and on blood chemistry 200 days after therapy SGOT, SGPT and alkaline phosphatase were within normal limits.
최흥재(Heung Jai Choi),김원호(Won Ho Kim),배형미(Hyung Mi Bae),함기백(Ki Baik Hahm),송시영(Si Young Song),전재윤(Chae Yoon Chon),강진경(Jin Kyung Kang),박인서(In Suh Park) 대한내과학회 1991 대한내과학회지 Vol.40 No.5
N/A Following portal vein occlusion, hepatopetal collaterals develop, undergo compensatory enlargement, bypass the obstructed extrahepatic occlusion and reconstitute the intrahepatic portal branches. Angiographically, the collateral veins are seen as multiple tortuous winding vessels in the porta hepatis and are described as a cavernous transformation of the portal vein. Ultrasonography is thc procedure of choice for the detection of various abnormalities of the portal vein and surrounding structures. Doppler ultrasonography adds hemodynamic information. We analyzed the clinical, ultrasonogaphic, angiographic, and Doppler ultrasonographic findings in 11 patients with cavernous transformation of the portal vein. Ultrasonographic findings suggesting cavernous transformation of the portal vein are 1) failure of visualization of the extrahepatic portal vein or portal division, 2) demonstration of high-level echoes in the region of the porta hepatis (diamond sign), and 3) visualization of multiple serpiginous vascular channels around the obstructed portal vein. Doppler ultrasonographic detection of flow signals from those collaterals, which are similar to the flow signals of the portal vein, confirms the diagnosis. We conclude that Doppler ultrasonography is a simple, noninvasive and very useful way to confirm the diagnosis of cavernous transfromation of the portal vein.