위암에서 Epstein-Barr Virus 연관성의 예측 인자

배한익,김영수,남상철,한만훈,정지윤,박선균,서인수 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.4

Background : It is important to detect Epstein-Barr virus (EBV) in the setting of gastric cancer so that early viral targeted therapy and prevention can be undertaken. The aim of this study was to investigate the predictive clinicopathologic factors for EBV-related gastric cancer. Methods : The archival tumor tissues from 335 patients with gastric cancer were examined using tissue microarray. The detection of EBV was performed using EBV mRNA in situ hybridization (EBV-ISH), and the results were compared against clinicopathologic factors. Results : EBV-related gastric cancers were identified in 21 of 335 investigated cases (6.27%). The anatomical predisposition of EBV-related cancers to manifest in the upper stomach was statistically significant (p<0.001). EBV-related cancers were almost always (20/21) accompanied by lymphoid stroma. No differences in age, sex, histologic differentiation, or T or N stage were noted between EBV-positive and EBV-negative gastric carcinomas. Conclusions : The association of EBV with gastric adenocarcinomas could be predicted when tumors with lymphoid stroma occurred in the upper stomach.

c-kit, PDGFRA의 면역조직화학적 발현 양상과 변이 분석을 통한 위장관기질종양 진단의 접근

배한익,김정식,김재훈,오현진,서인수,김종광,강병욱,유완식,정호영 대한병리학회 2010 Journal of Pathology and Translational Medicine Vol.44 No.2

Background : Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors in the gastrointestinal tract. Recently, many methods for the diagnosis of GIST have been developed including molecular diagnosis. Methods : We selected 90 cases of GIST that had presented at Kyungpook National University Hospital between 1998 and 2007. Tissue microarrays were made using core areas of tumor tissues. Immunohistochemical staining for c-kit, protein kinase C-theta, and platelet-derived growth factor receptor alpha (PDGFRA) was done. Direct sequencing of hot spot exonal areas for c-kit and PDGFRA were done using extracted DNAs of all 90 paraffin block tissues. Results : Among the 90 cases, 83.3% (75/90) were c-kit positive, 16.6% (15/90) were c-kit negative, 93.3% (84/90) were PDGFRA positive, and 6.6% (6/90) cases were PDGFRA negative. Fifteen cases of c-kit negative GIST included 1 case of PDGFRA negative and 5 cases of PDGFRA negative GIST were ckit positive. The one case in which both c-kit and PDGFRA were negative, showed a c-kit mutation in exon 11. Conclusions : Combined immunohistochemical staining of c-kit, discovered on GIST 1 (DOG1) and PDGFRA is helpful for the diagnosis of GIST. When all staining tests are negative for immunoreactivity, c-kit mutation analysis for exon 11, 9 should be done. Genotyping of kit and PDGFRA do not need to be examined initially, if it is only for the diagnosis of GIST.

위의 장형화생과 동반된 비정형 병소의 H-ras, erb B2, p53 단백의 발현 양상

배한익,김동훈,김정란 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.9

Gastric Mixed Adenoneuroendocrine Carcinoma with Squamous Differentiation: A Case Report

배한익,이채윤,조영민,권오경,유완식,김미선,서안나 대한병리학회 2016 Journal of Pathology and Translational Medicine Vol.50 No.4

주산기 심근병증 1 부검례

배한익,박동일,김정란 東國大學校 1993 東國論叢 Vol.32 No.-

Peripartum cardiomyopathy (PPCM) is a rare form of dilated cardiomyopathy. Onset is from the last trimester of pregnancy to 5 months postpartum. Diagnosis is made by excluding other causes of acute heart failure, such as infections or toxins, and by determining that the patient does not have an underlying cardiac problem that has been unmasked by pregnancy. Its clinical and hemodynamic findings are similar to idiopathic dilated cadiomyopathy : however, peripartum cardiomyopathy shows a high incidience of myocarditis. In addition to congestive heart failure, the occurrence of complications such as sepsis, hepatorenal failure, and arrhythmia were the major causes of death. We experienced an autopsy case of peripartum cardiomyopathy, which is the first in Korea, recently at Pohang hospital of Dongguk University. And we report a pathologic findings of that case with brief review of literatures. The major cause of death in this case is carbon monoxide intoxication with an accelerating association of peripartum cardiomyopathy.

1998년도 대구지역 법의 부검 통계

배한익,김은석,채종민,조현정,곽정식 大韓法醫學會 1999 대한법의학회지 Vol.23 No.2

This is a report revealing statical cause of death brought to the department of forensic medicine, Kyungpook National University School of Medicine based on the result of autopsy. The summary of result is as follows. 1. The number of autopsy made during 1998 were 356, of these 225 cases(63.2%) were male, and 131(36.8%) were female. 2. 30's, 40's, 20's and 50's was the order according to the number of violent death, and in case of natural death, 30's, 50's, 40's was the oder. 3. August is the period during which autopsies are made most, with December placing second, and the other months are similar. 4. In distributing deaths by cases, violent deaths were 241 cases(67.7%), natural deaths were 105(29.5%) and unknown cases were 10(2.8%). 5. For the violent deaths, deaths due to injury were 128 cases(5301%), asphyxia were 63(26.1%), intoxications were 30(12.4%) and abnormal temperature were 19(7.9%). According to relationship with deaths, homicide is outstanding with 101 cases(41.9%), while suicide and accidental death was the same with 62 cases(25.7%). 6. for death due to injury, death due to blunt injury were 48 cases, sharp force injury which was used most in case of homicide were 43. 7. For the asphyxia, drowning is the most 20 cases, 13 cases of which is accidental death. 8. The deaths of intoxication is the largest part of suicide, and most of intoxication were involved in agrochemical. 9. Of 105 cases of natural death, deaths due to the disease of cardiovascular system were 51 cases(48.6%), of which deaths due to myocardial infraction were 35 cases. 10. Of all autopsy, alcohol intake were checked out in 90 cases and 21 cases of medical controversy took places. 11. Of all violent deaths, the cases involved in the discord between man and woman occupied percentage of 9.6. Of these, homicides were 18 cases, with all sacrificed being females, and of 16 cases of suicide, 13 cases were female.

Genome Analysis Using Array-base Comparative Genomic Hybridization(CGH)

Bae, Han Ik 가톨릭 의과학연구원 2002 가톨릭 의과학연구원 국제학술대회 Vol.6 No.-

Array-based CGH is similar to preexisting chromosomal CGH but uses cytogenetically defined landmarks on the draft sequence of the human genome instead of normal metaphase chromosomes. The landmarks are large-insert clones mapped to chraomsome bands by fluorescence in situ hybridization(FISH). Each clone contains a sequence tag that is positioned on the genomic sequence.

후종격 응모막암종 -1 례 보고-

김수성,배한익 대한흉부심장혈관외과학회 1997 Journal of Chest Surgery (J Chest Surg) Vol.30 No.4

원발성으로 종격동에서 발생한 융모막암종은 드문 질환으로 젊은 남자에서 주로 발견되었으며, 기침, 흉통, 여성형 유방 등의 증상을 보이는 것으로 알려졌다. 33세 여자로 약 3개월 전부터 가벼운 기침으로 시작하였으나 심한 호흡곤란과흉통증 등 증상이 급격히 악화되어 내원하였다. 방사선학적 검사상 후종격에서 직경 13cm크기의 종양이 발견되었고, 혈중 $\beta$-HCG가 20만 mIU 이상 증가하였으며, 적출된 종양은 $\beta$-HCG에 대한 면역조직화학적 검사상 양성반응을 보였다. 융모막암종 절제술을 받고 EMA-CO투여 받은후 약 7개월만에 뇌에 전이된 종양 때문에 뇌출혈을 일으켜 다시 뇌종양 적출술을 받았다. 환자는 그 후 약 7개월동안 두통외의 증상은 없었고 흉부단순촬영상 종격종양의 재발소견도 없었다. Primary mediastinal choriocarcinoma is characteristically seen in young males presenting with the symptomes of cough, chest pale, and gynccomastia. A 33-year-old woman was admitted to the hospital because of severe dyspnea and chest pain which was aggravated rapidly 2 or 3 days ago. Posterior mcdiastinal mass measuring about 1 cm in diameter was seen in Chest P-A, left lateral view of chest, and chest CT. Serum $\beta$-HCG level was markedly elevated up to 200, 000 mIxt. Whole body CT and other studies could not find any lesion on ovary and uterus. But, a single nodule nEeasuring about 1 cm in diameter was identified in the brain CT. The tumor cells (syncytiotrophoblastic cells) from resected mass revealed positivity on i histochemical staining for $\beta$-HCG. She was treated with EMA-CO after resection of tumor, But, 7 months later, she was readmitted and showed cerebral hemorrhage due to metastatic choriocarcinoma. She was operated again for the brain tumor, and was doing well for further 7 months.

內膜1號丸 子宮內膜症 白鼠의 治療效果에 對한 硏究

권은정,배한익,이태균 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

Endometriosis is characterized by ectopic growth of tissue resembling endometriosis in various extrauterine locations. So we surgically implanted endometrial tissue to serosal wall of small intestine in rats. After that we administrated Naemak-1-ho-hwan for 10, 20, 40, 60, 80, 100 days. The results were as follows: 1. After 4weeks we could observe tissue resembling endometrium in serosal wall of small intestine. Endometrial tissue which was located serosal wall of intestine had the function of growing, secreting and bleeding. 2. The size of the endometrial tissue in the Naemak-1-ho-hwan administrated group was significantly reduced when we compared with water administrated group (control group) . Further more, as the time goes on, the effect of Naemak-1-ho-hwan much better. 3. In case of infected group, we could find abscess and fibriotic change, also like that case Naemak-1-ho-hwan was not effect. 4. There was no difference between Naemak-1-ho-hwan administrated group and control group in the shape of primordial follicle, primary follicle, maturing primary follicle and ovary size. So these results suggest that Naemak-1-ho-hwan will be effect for the patient who suffer from slight endometriosis.

Endotoxin 投與로 因한 肺胞細胞系의 變化에 關한 超微形態學的 硏究

최동수,배한익,김용진,김중길,곽정식,손태중 慶北大學校 醫科大學 1983 慶北醫大誌 Vol.24 No.2

저자는 endotoxin으로 인한 폐포세포계의 상해기전의 일단을 알아보기 위하여 Sprague-Dawley계 숫놈 흰쥐에 S. typosa O 901 endotoxin을 체중 100gm당 2㎎을 복강내에 주입하고 시간경과에 따라 폐에서 볼 수 있는 형태학적 변화를 광학 및 전자현미경으로 검색하고 또 ruthenium red법에 의한 폐포 피복층의 산성점액 다당류의 동태를 전자현미경적 세포화학적 측면에서 아울러 검색하였다. 그 성적을 요약하면 다음과 같다. 광학 현미경으로는 폐포벽의 모세혈관에서는 혈전 형성, 내피세포의 종창 및 충혈과 간질에서는 부종, 출혈, 세포침윤 및 상피세포의 박탈 등 변화가 관찰되었는데, 이것은 endotoxin 주입 2시간째부터 나타나기 시작하여 시간이 경과함에 따라 점차 심해지는 경향이었다. 무기폐 및 폐기종은 endotoxin 투여후 3시간째부터 나타나기 시작하였다. 전자현미경으로는 30분 내지 1시간부터 모세혈관의 확장과 더불어 모세혈관 내강에는 변성된 백혈구 적혈구 및 혈소판으로 된 미세혈전이 출현하고 1시간째부터는 내피세포에서는 종창, pinocytotic vesicle의 증가, mitochondria의 변성, ER의 종창, lysosome의 증가, 내피세포 간격의 확장 및 기저막 두께의 불규칙과 대식세포 내의 primary lysosome의 감소 및 secondary lysosome의 증가가 일어나고, 2시간째에는 Ⅰ형 및 Ⅱ형 폐포세포에 있어서는 mitochondria의 종창, osmiophilic inclusion body의 공포화 및 숫적 감소, RER의 확장, microvilli의 감소, 폐포강내의 적혈구, 대식세포, Ⅱ형 폐포세포의 출현 등이 관찰되었으며, 폐포 피복층의 산성점액다당류는 endotoxin 투여후 2시간째부터 감소하기 시작하였다. 이상의 변화들은 일단출현하면 시간경과에 따라 더 현저해지는 경향이 있다. 이상의 연구 성저으로 보아 endotoxin 투였시의 폐포 세포계의 상해 기전은 미소순환계에 장해가 먼저 일어나고, 이를 기반으로 하여 Ⅰ형 및 Ⅱ형세포의 변성, 산성점액다당류의 감소, 폐수종 및 무기폐 등 다양한 병리학적 변화가 일어나게 되는 것이라고 생각된다. To investigate the pathogenesis of injury in alveolar wall induced by endotoxin, the author administered 2㎎ of S. typhi O 901 endotoxin per 100 gm of body weight intraperitoneally to male Sprague-Dawley rats. The morphological changes were periodically observed by means of light microscopy, electron microscopy, and cytochemical electron microscopy; ruthenium red stain for identification of acid mucopolysaccharide on alveolar surface. Light microscopically, the changes occurred in alveolar wall were thrombosis, congestion, and swelling of endothelial cells in alveolar capillaries, edema, hemorrhage, and inflammatory infiltration in interstitium, and excoriation of epithelia. These changes began to appear at 2 hours after endotoxin injection and were more pronounced with time. Atelectasis and emphysema began to appear at 3 hours after endotoxin injection. Electron microscopically, at 30 minutes to 1 hour after injection, microthrombi composed of degenerated leukocytes, red cells and platelets, and capillary dilatation appeared. At 1 hour after injertlon endothelial cell showed swelling, increase in number of pinocytotic vesicles, degradation of mitochondria, dilatation of endoplasmic reticulum, increase in number of lysosomes, and widening of intercellular spaces. The basement membrane was irregular in thickness. The macro phages showed decrease in number of primary lysosomes and increase of secondary lysosomes. At 2 hours after injection, type Ⅰ and Ⅱ pneumocytes showed swelling of mitochondria, vacuolization and decrease in number of osmiophilic inclusion bodies, dilatation of RER, and reduction df microvilli. In the alveolar spaces, red cells, leukocytes, macrophages, exocoriated type Ⅱ pneumocytes appeared. Acid mucopolysaccharide on alveolar surface began to reduce in amount at 2 hours after injection. These changes, once appeared, tended to more pronounced with time. At 5 hours after injection of endotoxin, the most of rats in this group were died, the light microscopic changes were severe congestion, hemorrhage, capillary thrombosis, and atelectasis. Electror microscopic changes were appearance of microthrombi composed of degenerated leukocytes, red cells, and platelets, degradation and destruction of endothelial cells, degradation of type Ⅰ and Ⅱ pneumocytes with excoriation degeneration of macrophages, deposition of fibrins, osmiophilic inciusion bodies, red cells, and other degraded cell organelles in alveolar spaces. From the above results, it is conceived that pathogenesis of injury in alveolar wall induced by endotoxin is microcirculatory derangement as the earliest event, and as a result degeneration of type Ⅰ and Ⅱ pneumocytes, decrease of acid mucopolysaccharide, pulmonary edema, atelectasis, and other various pathologic changes could be arose.