우리나라에서 확보한 해양미생물과 미세조류에 대한 기초생리활성 연구

배승섭,권용민,정다운,유운종,조기철,조은서,정윤희,박윤경,안혜미,이대성,박진수,이재욱,오동찬,오기봉,조은지,박상익,전유진,이효근,김근용,남상집,최혁재,판철호,최그레이스 (사)한국해양생명과학회 2023 한국해양생명과학회지 Vol.8 No.2

우리나라 여러 해양환경 지역으로부터 확보한 370주의 해양세균, 균류, 미세조류로부터 기초생리활성(항산화, 항염, 항균, 항암, 항바이러스)을 조사하여 채집지, 분리원, 종(種) 수준에서의 활성결과를 비교하였다. 해양세균의 경우, 일반적으로 유용성이 많이 알려진 Streptomyces 속과 Bacillus 속에 속하는 균주들이 두드러진 강한 효능이 관찰되었고, 주로 해양퇴적물로부터 유용한 자원을 분리할 수 있었다. 해양균류와 미세조류의 경우에도 종 특이적으로 활성이 강하게 나타나는 결과를 확인할 수 있었고, 효능 특이적으로 활성을 보이는 결과도 얻을 수 있었다. 이러한 결과를 바탕으로 추후 특정질병에 선택적으로 효능을 보이는 화학물질 연구 또는 자원 기반 연구 수행 시 유용성을 전제로 한 자원 확보 전략 수립과 산업 활성화를 위한 전략소재로 우선적 접근이 용이할 수 있는 연구결과라 생각된다. 또한, 이들 결과를 해양바이오 뱅크를 통한 분양소재로 활용함으로써 학계, 산업계에서 활용하여 해양바이오산업 활성화에 좀 더 빠른 접근을 도울 수 있다고 생각한다. Basic bioactivities (antioxidant, anti-inflammatory, antibacterial, anticancer, antiviral) were investigated from 370 strains of marine bacteria, fungi, and microalgae obtained from various marine environmental regions in Korea, and the activity results were obtained at the collection site, isolation source, and species level was compared. In the case of marine bacteria, strains belonging to the generally useful genera Streptomyces and Bacillus were observed to have particularly strong efficacy and useful resources were mainly isolated from marine sediments. In the case of marine fungi and microalgae, results showing strong species-specific activity were confirmed, and results showing efficacy-specific activity were also obtained. Based on these results, it is a research result that can facilitate priority access as a strategic material for industrial revitalization and the establishment of a strategy to secure resources based on usefulness when conducting research on chemicals that are selectively effective against specific diseases or when conducting resource-based research. In addition, we believe that by using these results as material for sale through the Marine BioBank(MBB), academia and industry can use them to help accelerate the revitalization of the marine bio industry.

Complete genome sequence of a novel polysaccharide-degrading marine bacterium, Echinicola strongylocentroti MEBiC08714T

배승섭,정윤희,우정희,백경화 한국미생물학회 2019 미생물학회지 Vol.55 No.4

Echinicola strongylocentroti MEBiC08714 T isolated from sea urchin Strongylocentrotus intermedius can degrade polysaccharides like agar, alginate, and starch. The genome was sequenced to identify the genomic basis of this activity. The genome is composed of 6,252,144 bp, 4,695 protein-coding sequences, and 44.1 mol% G + C content. The strain MEBiC08714 T encodes a large set of carbohydrate active enzymes including 187 glycosyl hydroxylases and 22 polysaccharide lyases. The large diversity of hydrolytic enzymes supports the use of E. strongylocentroti MEBiC08714 T as a candidate for biotechnological applications in enzymatic polysaccharide conversion.

Immobilization of the Hyperthermophilic Archaeon Thermococcus onnurineus Using Amine-coated Silica Material for H₂ Production

배승섭,나정걸,이성목,강성균,이현숙,이정현,김태완,Bae, Seung Seob,Na, Jeong Geol,Lee, Sung-Mok,Kang, Sung Gyun,Lee, Hyun Sook,Lee, Jung-Hyun,Kim, Tae Wan The Korean Society for Microbiology and Biotechnol 2015 한국미생물·생명공학회지 Vol.43 No.3

초고온성 고세균 Thermococcus onnurineus NA1은 개미산, 일산화탄소, 또는 전분 등을 이용해서 수소를 생산하는 것으로 알려져 있다. 본 연구에서는 T. onnurineus NA1의 고정화 세포를 이용한 수소생산을 고찰하였다. 고정화 실험결과, T. onnurineus NA1은 표면에 아민기가 코팅된 규조토 담체에 정전기적 인력에 의해 효과적으로 고정화되었고, 1 g의 담체에 고정화 될 수 있는 최대 세포의 양은 71.7 mg-dcw로 확인되었다. 고정화 세포를 이용한 세 번의 반복회분식 배양을 통해 개미산으로부터 수소생산 특성을 고찰하였고, 그 결과 배양이 반복됨에 따라 고정화 세포 농도의 증가에 기인하여 초기수소생산속도가 2.3 에서 4.0 mmol l⁻¹ h⁻¹로 상당량 증가됨이 관찰되었다. 따라서, T. onnurineus NA1의 고정화세포 시스템은 수소생산을 위한 좋은 대안이 될 수 있을 것으로 사료된다. 본 연구는 초고온성 고세균의 고정화세포를 수소생산에 적용한 첫 번째 사례이다. Previously we reported that the hyperthermophilic archaeon, Thermococcus onnurineus NA1 is capable of producing hydrogen (H₂) from formate, CO or starch. In this study, we describe the immobilization of T. onnurineus NA1 as an alternative means of H₂ production. Amine-coated silica particles were effective in immobilizing T. onnurineus NA1 by electrostatic interaction, showing a maximum cell adsorption capacity of 71.7 mg-dried cells per g of particle. In three cycles of repeated-batch cultivation using sodium formate as the sole energy source, immobilized cells showed reproducible H₂ production with a considerable increase in the initial production rate from 2.3 to 4.0 mmol l⁻¹ h⁻¹, mainly due to the increase in the immobilized cell concentration as the batch culture was repeated. Thus, the immobilized-cell system of T. onnurineus NA1 was demonstrated to be feasible for H₂ production. This study is the first example of immobilized cells of hyperthermophilic archaea being used for the production of H₂.

Complete genome sequence of a zeaxanthin-producing marine bacterium, Fulvivirga lutea S481T

배승섭,정윤희,권용민,정다운,유운종,최그레이스 한국미생물학회 2023 미생물학회지 Vol.59 No.4

A zeaxanthin-producing marine bacterium, Fulvivirga lutea S481T was isolated from surface seawater. The complete genome of F. lutea S481T consists of 4,137,993 bp with a G + C content of 37.3 mol% and contains 3,616 protein-coding sequences, 37 tRNA genes, and 6 rRNA genes. Genome analysis revealed five genes involved in the zeaxanthin synthesis pathway. This genome information will provide fundamental knowledge for synthetic biology and metabolic engineering studies on zeaxanthin carotenoid synthesis.

Complete genome sequence of Microbulbifer agarilyticus GP101 possessing genes coding for diverse polysaccharide-degrading enzymes

정재준,배승섭,정다운,백경화,Jung, Jaejoon,Bae, Seung Seob,Chung, Dawoon,Baek, Kyunghwa The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3

Microbulbifer agarilyticus GP101은 소라(Turbo cornutus)의 내장에서 분리되었으며 해조류 유래 다당류인 한천, 알긴산, ${\kappa}$-카라기난을 분해하는 특징이 있다. GP101 균주의 유전체는 4,255,625 bp 크기로 3,458개의 코딩 서열을 포함하며 55.4%의 GC 함량을 가진다. BLASTP 분석 결과 7개의 agarase, 5개의 alginate lyase, 10개의 glucanase, 4개의 chitinase, 2개의 xylanases, 1개의 ${\kappa}$-carrageenase, 1개의 laminarinase의 존재를 확인하였다. M. agarilyticus GP101의 유전체 정보는 다당류의 생물전환 공정에 이용할 수 있는 유전 정보를 제공할 수 있을 것이다. Microbulbifer agarilyticus GP101 was isolated from the gut of a marine invertebrate Turbo cornutus and capable of degrading polysaccharide such as agar, alginate, and ${\kappa}$-carrageenan constituting algal cell wall. To obtain genomic basis of polysaccharide-degrading activity, we sequenced genome of strain GP101. The genome consists of 4,255,625 bp, 3,458 coding sequences with 55.4% G + C contents. BLASTP search revealed the presence of seven agarases, five alginate lyases, ten glucanases, four chitinases, two xylanases, one ${\kappa}$-carrageenase, and one laminarinase. The genomic data of strain GP101 will provide potential uses in the bioconversion process of diverse polysaccharide into bioenergy and biochemicals.

Complete genome sequence of Tamlana sp. UJ94 degrading alginate

정재준,배승섭,정다운,백경화,Jung, Jaejoon,Bae, Seung Seob,Chung, Dawoon,Baek, Kyunghwa The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

Tamlana sp. UJ94는 해수로부터 분리되었으며 알긴산을 분해할 수 있다. 알긴산 분해 관련 특성을 이해하기 위해 이 세균의 유전체를 분석하였다. UJ94의 유전체는 4,116,543 bp의크기로 3,609개의 코딩서열을 가지고 있으며 35.2 mol%의 G + C 함량을 가진다. BLASTp 검색 결과 9개의 alginate lyase 외에도 6개의 agarase, 5개의 amylase, 4개의 carrageenase, 1개의 cellulase, 4개의 pectate lyase, 7개의 xylanase의 존재가 예측되어 UJ94의 다양한 다당류 분해 능력을 암시하였다. Tamlana sp. UJ94의 유전체는 생물전환 공정에 사용할 수 있는 다당류 분해 유전자를 제공할 수 있을 것이다. Tamlana sp. UJ94 isolated from seawater can degrade alginate. To identify the genomic basis of this activity, the genome was sequenced. The genome was composed of 4,116,543 bp, 3,609 coding sequences, and 35.2 mol% G + C content. A BLASTp search predicted the presence of 9 alginate lyases as well as 6 agarases, 5 amylases, 4 carrageenases, 1 cellulase, 4 pectate lyases, and 7 xylanases, indicating its ability to degrade diverse polysaccharides. The genome of strain UJ94 is a source of polysaccharide-degrading enzymes for bioconversion processes.

Marine-Derived Fungi in Korea

권용민,배승섭,최은주,임지연,정윤희,정다운 한국해양과학기술원 2021 Ocean science journal Vol.56 No.1

Studies on marine fungi (termed as ‘marine-derived fungi’ in this paper) have been significantly increasing worldwide because of the critical role displayed by marine fungi in the marine ecosystem and their potential as sources of bioactive compounds. Korea is surrounded by three seas containing abundant marine resources. In this review, to facilitate research and applications of marine fungi, we summarized previously studied marine fungi in Korea focusing on published research articles in peer-reviewed journals over the last two decades. Approximately 79% of the studies (66 articles) were published in the 2010s and described a total of 818 strains (isolates) of 266 species belonging to 3 phyla, 8 classes, 21 orders, 43 families, and 76 genera. The most prevalent fungi belong to the genera Penicillium/Eupenicillium/Talaromyces, accounting for 40.2% of all species (107 species), followed by Aspergillus/Eurotium/Neosartorya (13.9%, 37 strains). Approximately 67% of fungal strains were originated from the eastern (44.6%, 365 strains) and western coasts (22.1%, 181 strains), and their main isolation sources were coastal plants/halophytes, marine animals, sediment, and marine algae. More than 35% of the strains (292 strains) produced novel and/or bioactive compounds with enzymatic, antimicrobial, antioxidant, anti-cancer, anti-inflammatory, anti-diabetic, or multi-functional activities. This review provides insights into current knowledge and future perspectives regarding marine-derived fungi in Korea.

A study on the mass production of bio-hydrogen from carbon monoxide

김태완,이성목,배승섭,이현숙,이정현,나정걸,강성균 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0

In this study, a microbial fermentation process was developed to produce H2 from CO. For this, continuous fermentation of Thermococcus onnurineus was carried out in a 2.5 L stirred tank bioreactor with varying process variables. It was observed that if CO was not limited, both the cell growth and H2 production rate (HPR) were linearly correlated with dilution rate. The HPR has also been observed to increase linearly with CO supply rate or agitation speed, mainly due to an increase of gas-liquid mass transfer rate. Based on the lab-scale results, the pilot study was performed in a 1,000 L gas-lift type bioreactor using CO-containing industrial off-gas. As a results of continuous cultivation of T. onnurineus on steel mill by-product gas, the HPR reached a maximum value of 250 mmol/L/h, which corresponds to a daily H₂ production of 50 Nm³.

Hexabromobenzens 농후 배양에 따른 해면(Axinella sp.) 공생 미생물의 군집구조 변화

서현석,양성현,배승섭,이정현,권개경,Seo, Hyun-Seok,Yang, Sung-Hyun,Bae, Seung Seob,Lee, Jung-Hyun,Kwon, Kae Kyoung 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2

할로겐화 또는 탈할로겐화에 관여하는 미생물을 특정하고자 해양의 주된 할로겐화합물 생산자인 해면 공생미생물의 군집구조에 미치는 HBB (hexabromobenzene)의 영향을 밝히고자 했다. 혐기 marine broth 2216에서 100ppm HBB첨가 유무에 관계없이 검출된 균주들은 산호나 해면에서 유래한 클론들과 높은 유사도를 보였으며 그 중 Deltaproteobacteria에 속하는 Desulfovibrio marinisediminis와 99% 유사도를 보이는 클론이 우점하는 것으로 나타났다. Clostridia계통의 Fusibacter paucivorans와 유사도가 높은 클론과 Lentisphaerae에 속하는 균주들은 HBB에 의해 감소하는 반면 Clostridia계통의 Vallitalea guaymasensis와 유사도가 높은 균주들은 HBB가 있는 경우에만 검출되었다. 알려진 균주들과의 비교 결과로 볼 때 D. marinisediminis 및 V. guaymasensis 계통의 균주들이 할로겐화 및 탈할로겐화에 관여하는 것으로 추정된다. Bacteria associated with marine sponges seemed to be concerned in halogenation/dehalogenation process of natural compounds. In the present study, the effect of hexabromobenzene (HBB) on the community structure of bacteria associated with a marine sponge Axinella sp. from Chuuk State under anaerobic condition was investigated. Regardless of 100 ppm HBB, most of detected microorganisms displayed high similarity with clones reported from coral or sponges. Amongst, Desulfovibrio marinisediminis like clones were dominant. Clones affiliated with Lentisphaerae and Fusibacter paucivorans (Clostridia) were detected at the conditions without HBB but clones affiliated with Vallitalea guaymasensis (Clostridia) increased its proportion with HBB. From these results and previous reports clones affiliated with D. marinisediminis and V. guaymasensis seemed to be concerned in halogenation/dehalogenation process.

Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

류정화,김학준,배승섭,정춘구,공승표 한국수산과학회 2016 Fisheries and Aquatic Sciences Vol.19 No.2

This study was conducted to identify the general conditions for the isolation and in vitro culture of ovary-derived cells in rockfish (Sebastes schlegeli). The effects of three different enzymes on cell retrieval from ovarian tissues were evaluated first, and then the ovary-dissociated cells were cultured under various culture conditions, with varying basal media and culture temperatures, addition of growth factors, and/or culture types. We found that collagenase type I treatment was effective for cell isolation from ovarian tissues. From a total of 42 trials to evaluate the effects of basal media and culture temperatures on cell culture of ovary-dissociated cells, we observed that Leibovitz’s L15 medium was more supportive than Dulbecco’s modified Eagle’s medium for culture, and the cells could grow at all three temperatures tested, 15, 20, and 25 °C, at least up to passage 2. However, growth factor addition did not improve cell growth. Introduction of suspension culture after monolayer culture expanded the culture period significantly more than did monolayer culture alone. Our results may provide a basis for developing an in vitro system for S. schlegeli germline cell culture, which will ultimately lead to improvement of the species.