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호밀(Secale cereale L .)Esterase 동위효소의 유전적 연구
방재욱,최혜운 한국유전학회 1990 Genes & Genomics Vol.12 No.2
Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, whereas EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.
방재욱 한국유전학회 1988 Genes & Genomics Vol.10 No.2
The seedling proteins of rye were investigated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis. Also the difference of electrophoretic pattern related to the presence of B chromosomes was studied. In SDS-PAGE gels, mass differences in high molecular weight proteins were observed between plants with B and without B chromosomes. More than 90 polypeptide spots were scored on 2-D gels. In comparison of 2-D electrophoretic patterns between seedlings with and without B chromosomes, mass modifications were found in 3 polypeptides on gels.
호밀(Secale cereale L.) 染色體의 多型現像 分析
方在旭 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.1
The polymorphism of rye chromosomes studied by using a special graphic method based on the arm-ratio and length of the mitotic chromosomes. The chromosome complement of rye was consisted of 1 pair of satellite chromosome, 2 pair of median, 2 pairs of submedian, 1 pair of submedian-subterminal, and 1 pair of subterminal chromosomes. The polymorphic differences in length were more prevalent than in arm-ration. Significant differences in polymorphism were found in chromosome Ⅰand VⅡ, chromosome Ⅵ seemed to be the most stable among rye chromosomes.