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굴(Crassostrea gigas), 멍게(Halocynthia roretzi) 및 해삼(Apostichopus japonicus)에서 분리한 대장균의 항균제 내성 특성
박큰바위,류아라,김송희,함인태,권지영,김지회,유홍식,이희정,목종수,Park, Kunbawui,Ryu, A Ra,Kim, Song Hee,Ham, In Tae,Kwon, Ji Young,Kim, Ji Hoe,Yu, Hong Sik,Lee, Hee Jung,Mok, Jong Soo 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5
This study evaluated the abundance of fecal coliforms in oysters Crassostrea gigas, sea squirts Halocynthia roretzi and sea cucumbers Apostichopus japonicus in fisheries along the coast of Korea in 2014, and investigated the prevalence of antimicrobial resistance in Escherichia coli isolated from these fishery products. The ranges of fecal coliforms found in oysters, sea squirts and sea cucumbers were <18-20, <18-330 and <18-3,300 MPN (most probable number)/100 g, respectively. Sea squirts contained the greatest range of E. coli (<20-140 MPN/100 g), followed by sea cucumbers (<20-130 MPN/100 g) and oysters (<20-20 MPN/100 g). A total of 26 strains of E. coli were isolated from 34 sea squirt, 25 sea cucumber and 13 oyster samples. Strains thus isolated were tested for their susceptibility to 22 antimicrobial agents used in Korea for medical or veterinary therapy. E. coli isolates showed the greatest resistance to ampicillin (84.6%), followed by trimethoprim (34.6%), nalidixic acid (34.6%), tetracycline (30.8%), pipemidic acid (26.9%), streptomycin (23.1%), chloramphenicol (23.1%), trimethoprim/sulfamethoxazole (23.1%), and gentamicin (15.4%). Resistance to at least one antimicrobial agent was present in 88.5% of E. coli isolates. Of the 26 isolated, six strains (23.1%) were resistant to multiple antimicrobial agents.
2013-2016년 경남 연안 해수 및 패류에서 병원성 비브리오균의 계절적 및 지역적 변동
박큰바위,목종수,권지영,류아라,심길보 한국수산과학회 2019 한국수산과학회지 Vol.52 No.1
The seasonal and spatial variation of pathogenic Vibrio species, such as V. parahaemolyticus, V. vulnificus, V. alginolyticus, and V. cholerae were investigated in seawater and in bivalves off the Gyeongnam coast of Korea, which is an important area for shellfish production, during the period 2013-2016. V. parahaemolyticus, V. vulnificus, V. alginolyticus, and V. cholerae were detected in 12.1%, 5.2%, 15.4%, and 0.9% of seawater samples, respectively. V. parahaemolyticus, V. vulnificus, V. alginolyticus, and V. cholera were detected in 21.9%, 7.1%, 12.2%, and 0.0% of shellfish samples, respectively. The Vibrio spp. in seawater and bivalve samples were detected at high levels during the summer to early autumn; however, the levels were low during the winter. Therefore, their occurrence was seasonally dependent and correlated with high water temperature, which is also the biggest factor contributing to foodborne outbreaks associated with Vibrio. Relatively high detection rates of the strains were also found in the sea area that was continually exposed to inland wastewater. Our findings show that continuous monitoring is needed to reveal the patterns of occurrence of these pathogens from marine samples collected off the Korean coast, to reduce seafood-borne outbreaks caused by Vibrio.
박큰바위,목종수,권지영,류아라,김송희,이희정 한국수산과학회 2018 Fisheries and Aquatic Sciences Vol.21 No.1
Background: Vibrio parahaemolyticus is one of the most common causes of seafood-borne illnesses in Korea, either directly or indirectly, by consuming infected seafood. Many studies have demonstrated the antibiotic susceptibility profile of V. parahaemolyticus. This strain has developed multiple antibiotic resistance, which has raised serious public health and economic concerns. This article reviews the food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of V. parahaemolyticus in Korea during 2003–2016. Main body: V. parahaemolyticus infections appeared to be seasonally dependent, because 69.7% of patient infections occurred in both August and September during 2003–2016. In addition, the occurrence of V. parahaemolyticus in marine environments varies seasonally but is particularly high in July, August, and September. V. parahaemolyticus isolated from aquaculture sources on the Korean coast varied in association with virulence genes, some did not possess either the tdh (thermostable direct hemolysin) or trh (tdh-related hemolysin) genes, and a few were positive for only the trh gene or both genes. The high percentage of ampicillin resistance against V. parahaemolyticus in the aquatic environment suggests that ampicillin cannot be used to effectively treat infections caused by this organism. Short conclusion: This study shows that the observed high percentage of multiple antibiotic resistance to V. parahaemolyticus is due to conventionally used antibiotics. Therefore, monitoring the antimicrobial resistance patterns at a national level and other solutions are needed to control aquaculture infections, ensure seafood safety, and avoid threats to public health caused by massive misuse of antibiotics.
2018-2019년 남해안 어류양식장에서 분리한 대장균(Escherichia coli)의 항생제 내성 특성 및 비교
박큰바위,정연중,유홍식,조미라,정연겸,손광태,목종수 한국수산과학회 2023 한국수산과학회지 Vol.56 No.2
Antimicrobial resistance patterns of Escherichia coli strains isolated from 30 seawater samples and 30 Korean rockfish Sebastes schlegelii collected from fish farms off the South Coast of Korea in 2018–2019, were investigated. The isolated E. coli (154 strains) showed the highest antimicrobial resistance to tetracycline (44.8%) followed by chloramphenicol (38.3%), streptomycin (37.6%), and trimethoprim/sulfamethoxazole (35.7%). The antimicrobial resistance rate was higher in E. coli strains isolated from Korean rockfish than those from seawater. Multiple antibiotic resistance (MAR) against at least three antimicrobials (MAR index: ≥0.2) was observed in 35.6% and 79.2% of the seawater and Korean rockfish isolates, respectively. In conclusion, continuous monitoring is required to identify changes in antimicrobial resistance and their correlations in the aquatic environment and products.
남해안 패류양식장에서 분리된 대장균(Escherichia coli)의 항균제 내성
박큰바위 ( Kunbawui Park ),박준용 ( Jun Yong Park ),조미라 ( Mi Ra Jo ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),오은경 ( Eun Gyong Oh ),신순범 ( Soon Bum Shin ),김연계 ( Yeon Kye Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.5
This study evaluated the impact of antimicrobial resistant bacteria produced by inland pollution sources on coastal areas, and investigates antimicrobial resistance profiles of bacteria isolated from marine filter feeders. A total of 107shellfish (short-necked clams Ruditapes philippinarum, ark shells Scapharca broughtonii, and oysters Crassostrea gigas) were collected from the southern coast of Korea, from which 204 Escherichia coli strains were isolated. The antimicrobial susceptibility patterns of isolated strains were analyzed for 15 antimicrobial agents used in Korea for clinical or veterinary therapy. Antimicrobial resistance was present in 44.6% of E. coli isolates against at least one antimicrobial agent. The rate of antimicrobial resistance in the Narodo area was higher than isolates from the Gangjinman area and Kamak Bay. E. coli isolates had a higher rate of resistance against: tetracycline (29.9%), streptomycin (25.5%), and trimethoprim (14.2%). Of 204 isolates, 29 (14.2%) were resistant to multiple antimicrobial agents.
강우 발생에 따른 남해군 봉천 방출수가 강진만 해역의 세균학적 수질에 미치는 영향 평가
박큰바위 ( Kun Ba Wui Park ),조미라 ( Mi Ra Jo ),이희정 ( Hee Jung Lee ),권지영 ( Ji Young Kwon ),손광태 ( Kwang Tae Son ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
We investigated the effect of the discharged water from Bong Stream, which is located in the drainage area of Gangjinman area on the bacteriological water quality in the coastal area after rainfall events. Following 12.5 mm of rainfall, water discharged from Bong Stream had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. On the other hand, after 23 mm rainfall, the density of fecal coliform at stations located in the designated area was higher than at stations located in the adjacent area. The degree of bacteriological contamination at the surveyed stations in the coastal area after rainfall events did not show a relationship with distance from the shoreline. These results indicate that the direction of spread and the range of contaminants from the drainage area were affected by tides at the time of the survey. Therefore, a detailed survey of the effects of tides on the diffusion characteristics of the contaminants from Bong Stream is needed to establish a proper management plan for the surveyed area.
가열처리 조건에 따른 오염굴(Crassostrea gigas) 중의 Male SpecificColiphage와 노로바이러스 농도변화
박큰바위 ( Kun Ba Wui Park ),박용수 ( Yong Su Park ),권지영 ( Ji Young Kwon ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ),김풍호 ( Poong Ho Kim ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.6
Noroviruses (NoV) are known to cause acute epidemic gastroenteritis worldwide. Outbreak strains are predominantly genogroup I (GI) and genogroup II (GII) in oysters Crassostrea gigas. We investigated the changes in concentration of male specific coliphage (MSC) and NoV under heat treatment of the naturally contaminated oyster, Crassostrea gigas. After heat treatment for 5 min in 85℃, no viable MSC was detected. The concentrations of GI and GII NoV decreased by 1.65 log and 2.25 log, respectively, following heat treatment for 5 min at 100℃. Moreover, both GI and GII NoV were completely deactivated by heat treatment for 10 min at 100℃. Therefore, in order to reduce the risk of norovirus infection from contaminated oysters, immersion in boiling water for at least 10 min is recommended.
한산거제만 및 자란만사량도 해역 육상오염원 배출수에서 분리한 대장균의 항균제 내성 패턴
박큰바위 ( Kunbawui Park ),김송희 ( Song Hee Kim ),함인태 ( In Tae Ham ),류아라 ( A Ra Ryu ),권지영 ( Ji Young Kwon ),김지회 ( Ji Hoe Kim ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),목종수 ( Jong Soo Mok ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.1
We investigated patterns of antimicrobial resistance in Escherichia coli isolated from the water discharged from inland pollution sources in the Hansan-Geojeman and Jaranman-Saryangdo areas of Korea. A total of 217 strains of E. coli were isolated from 23 point-sources. These strains were tested for their susceptibility to 16 antimicrobial agents used in Korea for medical or veterinary therapy. The highest level of antibiotic resistance among the isolated strains was to tetracycline 10.6%, followed by ampicillin (3.2%), nalidixic acid (2.8%), rifampin (1.8%), trimethoprim (1.8%), trimethoprim/sulfamethoxazole (1.8%), chloramphenicol (1.4%), streptomycin (1.4%), cephalothin (0.5%) and gentamicin (0.5%). Resistance to at least one antimicrobial agent was present in 17.1% of the E. coli isolates. Only four of the isolated strains of E. coli showed multiple antibiotic resistance, which is defined as resistance to more than four antibiotics.
강우 발생에 따른 육상오염원이 나로도 해역의 세균학적 수질에 미치는 영향 평가
박큰바위 ( Kunbawui Park ),조미라 ( Mi Ra Jo ),김연계 ( Yeon Kye Kim ),이희정 ( Hee Jung Lee ),권지영 ( Ji Young Kwon ),손광태 ( Kwang Tae Son ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
This study evaluated the effect of the inland pollution sources on the bacteriological water quality in Narodo area after rainfall events. Following 16 mm of rainfall, the water discharged from Dohwa Stream and contaminants from Balpo village had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. In comparion, after 33 mm of rainfall, the density of fecal coliforms at stations located in the mouth of Dohwa stream and the discharg point of contamination sources located in Balpo village were lower than after 16 mm of rainfall. These results were obtained during an extreme high tide near the coast the contaminants discharged from Dohwa stream and Balpo village. Therefore, it is necessary to conduct a detailed survey to examine the dffects of tides on the diffusion characteristics of the contaminants discharged from Dohwa stream and Balpo village in order to manage of Narodo area efficiently.