Prognostic significance of preoperative 18F-FDG PET/CT in uterine leiomyosarcoma

박정열,이정원,이현주,이종진,문승환,강서영,천기정,정현훈 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.3

Objective: Uterine leiomyosarcoma (LMS) is a rare and aggressive disease with poor outcome. Due to its rarity and conflict of data, investigation on finding prognostic factor is challenging. The aim of the study was to investigate the prognostic significance of preoperative 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT) in uterine LMS. Methods: This was a retrospective observational cohort study in 3 tertiary referral hospitals. We retrospectively evaluated data from patients with pathologically proven uterine LMS who underwent preoperative 18F-FDG PET/CT scans at 3 institutions. The prognostic implication of PET/CT parameters and other clinico-pathological parameters on disease-free survival (DFS) and overall survival (OS) was evaluated. Results: Clinico-patholgical data were reviewed for 19 eligible patients. In the group overall, median DFS and OS were 12 and 20 months, respectively. As for the recurrence, large tumor size, and high tumor maximum standardized uptake value (SUVmax) were demonstrated as risk factors of recurrence. As for the OS, high tumor SUVmax was demonstrated as the unique risk factor. There were significant differences in tumor size, mitotic count, SUVmax, and DFS between patients with and without recurrence. Also, there were significant differences in tumor size, SUVmax, DFS, and OS between 2 subgroups stratified by cut-off SUVmax. Conclusion: SUVmax at preoperative 18F-FDG PET/CT was associated with worse outcome in patients with uterine LMS. In the preoperative setting, SUVmax can be a valuable non-invasive prognostic marker. Additionally, SUVmax can help identify highly aggressive uterine LMS and may help in adjusting standard treatment toward an individualized, risk-adapted treatment.

Ethanol의 低溫心臟에 對한 抗細動作用에 關한 硏究

朴廷烈,李聖行 慶北大學校 醫科大學 1974 慶北醫大誌 Vol.15 No.1

This experimental study was performed to clarify the antifibrillatory effects of intravenous infusion of ethanol upon hypothermic hearts. Thirty six mongrel dogs were anesthetized with thiopental sodium and were ventilated with mechanical respirator throughout the experiment. Ethanol, 2.0g/kg, diluted with 5% dextrose solution was intravenously administered in divided doses for the expermental dogs and same amount of 5% dextrose solution for control dogs. Animals were cooled with ice water and rewarmed with hot water. Heart rates and cardiac arrhythmia were checked by ECG. Central venous pressure, right ventricular and pulmonary arterial pressures were measured by means of cardiac catheterization. Animals were divided into two groups. Group 1. Twenty dogs were cooled until death. Among those, 10 experimental dogs were infused ethanol intravenously. Group 2. Sixteen dogs were cooled down to body temperature of 20℃, when cardiac inflow and out-flow were occluded for the period of 15 minutes. Then rewarmed the animals. The results were as follows. 1. Heart rates, arterial pressure, right ventricular and pulmonary arterial pressures were markedly decreased during the period of cooling. Ethanol infusion showed no significant influence upon those decreases. 2. Central venous pressure increased to the level of right heart failure of the cooled animal, and ethanol revealed no protective action on cardiac impairment. 3. In group 1, 10% of ventricular fibrillation was seen among the ethanol infused dogs while 30% of ventricular fibrilltion among the control group. In group 2, there was no significant difference of ventricular fibrillation between ethanol and control groups. 4. Ethanol blood level at 20℃ of body temperature was 636mg%. 5. It was suspected that ethanol failed to protect the hypothermic heart from ventricular fibrillation when cardiac inflow were occluded for the 15 minutes of period.

일상재구성법을 통한 경력단절 여성의 일상생활과 정서경험 분석

박정열,손영미 여가문화학회 2015 여가학연구 Vol.13 No.2

This study was conducted to investigate what was career interrupted women’ main activities in everyday life and what and how they felt in those activities. 724 married career interrupted women were participated in this DRM(day reconstruction method) research. The major findings of this study were as follows. While they were more inclined to experience positive emotion, they were less inclined to experience negative emotion. Secondly, only a few of participants selected ‘learning’, ‘job hunting activities’ among everyday activities. Worst of all, it was revealed that they felt anxious, stressful and worried as they were doing job hunting activities. Thirdly, the most frequent activities such as cooking, cleaning, childcare etc were showed to be felt less positive and more negative emotions. Forth, participants experienced different positive and negative emotions depending on person(husband, children, friend etc). It was worthy of notice that their children were the objects that they had an ambivalent emotion that is affective and confident as well as tired, bored and anxious. Finally, It was revealed that am 8-11 and pm 6-8 were burnout time zones where participants experienced the most negative and the least positive emotion all over the day. 본 연구는 일상재구성법을 활용하여 경력단절 여성의 일상생활의 주요 활동(가사 및 육아, 여가, 학습 및 구직활동 등)을 탐색하고, 정서경험의 질을 측정하는 것을 목적으로 한다. 이를 통해 이들의 현재의 행복수준을 진단하고, 재취업과 관련한 시사점을 도출하고자 하였다. 직장을 그만 둔지 1년 이상 된 30대, 40대, 50대 경력단절 여성 724명의 자료가 분석되었으며, 그 결과는 다음과 같다. 먼저, 경력단절 여성들은 평상시 긍정적인 정서수준이 높고, 부정적인 정서수준은 낮은 것으로 나타났다. 다음으로, 경력단절 여성 중 ‘교육 및 학습’, ‘구직활동’에 참여하는 여성이 극소수인데, 참여하는 여성들도 학습활동이 구직활동으로 이어지지 못하는 경향을 보였다. 구직활동은 경력단절 여성에게 상당한 걱정과 긴장, 스트레스를 주는 활동이었다. 셋째, 경력단절 여성들은 음식준비, 청소, 자녀보살피기 등의 활동에 가장 빈번하게 참여하면서도 그것에 대해 긍정 정서는 적게 느끼고, 부정 정서는 더욱 느끼는 경향을 보였다. 넷째, 주요 상호작용 대상자에 따라 느끼는 정서가 서로 다름을 알 수 있었다. 특히 자녀는 애정/우호, 유능/자신감을 주는 대상임과 동시에 피곤함, 지루함, 긴장/스트레스를 느끼게 하는 부정적인 정서대상으로 드러나 가장 양가적인 감정을 느끼는 대상이었다. 마지막으로, 남편의 출근과 자녀의 등교를 위해 바쁘게 움직여야 하는 8시~11시, 저녁식사를 준비하고 정리해야 하는 저녁 6시~8시가 경력단절 여성이 가장 높은 수준의 정서노동을 하는 시간대임을 확인할 수 있다.

Development and validation of LC-MS/MS for bioanalysis of hydroxychloroquine in human whole blood

박정열,주성수,권영이,김서진,장수길,송현호 충북대학교 동물의학연구소 2018 Journal of Biomedical and Translational Research Vol.19 No.4

This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative hydroxychloroquine-D4 as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column (50 mm × 4.6 mm, 2.6 μm) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 – 500 ng/mL for HCQ; 2 – 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.

Automated assessment of writing in a Korean learner corpus

박정열 국제한국어교육학회 2019 국제한국어교육학회 학술대회논문집 Vol.2019 No.-

금기어(禁忌語) 분석을 통한 한국인의 심층심리 탐색

박정열,최상진 한국심리학회 2003 한국심리학회지 일반 Vol.22 No.1

The purpose of this study is to investigate Korean's deep psychology reflected in tabooed words. The materials of this study were 625 Korean tabooed words. Contents analysis was administered and the major categories were extracted. The finding showed that human life, dream, animal/plant, and nature were the main theme of taboo. Especially human life related tabooed words were the most frequent. More specifically, the tabooed words related to human life were mainly about clothes/food/habitation, household effects, rite of passage, time, body, disease, human relationship, sex, and person. The tabooed words related to clothes/food/habitation and rite of passage were found more frequently than any others. In addition, contents analysis was administered on the consequences of breaking the taboo. Finally, all of these findings were discussed in relation to the Korean culture. 본 고는 금기(禁忌)가 사람들의 일상생활에서 생생하게 살아있는 문화적인 현상이며, 심층적인 한국인의 심리를 이루는 바탕이 된다는 판단 하에, 금기어 분석을 통해 한국인의 심층심리를 파악해보았다. 그 결과를 보면 전체 금기어 중에서 인간생활과 관련된 금기어가 가장 많았으며, 꿈, 동‧식물, 그리고 자연과 관련된 금기어들 순이었다. 전체 금기어의 내용 중에서 가장 많은 빈도를 차지했던 인간생활과 관련된 금기어들은 의식주, 가재도구, 통과의례, 시간, 신체부위, 질병, 대인관계, 남녀, 인물 등과 관련된 금기어들이었다. 그러나 이 중에서 특히 의식주와 통과의례에 관련된 금기어들이 단연 많은 비중을 차지하였다. 금기어의 후반 주절부 분석을 통해 과거 사람들이 인생에서 얻고자했던 가치를 확인 할 수 있었으며, 이러한 것들로는 무병장수, 풍요로운 삶, 자연과 순리에 따르는 삶, 그리고 화목한 가정과 관련된 것이 주종을 이루었다. 끝으로 이러한 금기가 오늘날 일반인들의 심리 속에 반영된 현상들에 대해 논의하였다.

마이크로 기술을 이용한 심장세포칩

박정열 대한기계학회 2010 기계저널 Vol.50 No.11

Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants

박정열,민현근,이홍규,Rameswor Maharjan,윤영남,이수헌 한국식물병리학회 2018 식물병연구 Vol.24 No.4

A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed for the detection of Clover yellow vein virus (ClYVV), Peanut mottle virus (PeMoV), and Tomato spotted wilt virus (TSWV), which were recently reported to infect soybean and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected and further evaluated to estimate the detection limits of uniplex, duplex, and multiplex RT-PCR. The multiplex RT-PCR assay could be a useful tool for the field survey of plant viruses and the rapid detection of ClYVV, PeMoV, and TSWV in leguminous plants.

Examinations on Solo-Diners’ Binge Eating Tendencies and Revisit Intentions

박정열 한국식공간학회 2018 식공간연구 Vol.13 No.3

As the number of people spending time alone increases, consumers are involving consumption activities by themselves. Following this trend, the number of people who dine alone at a restaurant increased, and solo-diners has been highlighted by restaurant industry practitioners and academic researchers. This study was designed to identify influential factors on solo-diners’ binge eating tendencies and revisit intentions. By collecting data from restaurant customers who have a recent experience of solo-dining, this study found that positive mood alleviation, food quality, and service quality reduced solo-diners’ binge eating tendencies. However, negative mood alleviation increased the binge eating tendencies. For solo-diners, positive mood reinforcement and food quality increased revisit intentions. Theoretical and practical implications are discussed in detail.

Beet western yellows virus (BWYV): Aspect of Outbreak and Survey, and First Complete Genome Sequence of a Korea Isolate of BWYV

박정열,김정선,이홍규,오종희,임승모,문제선,이수헌 한국식물병리학회 2018 식물병연구 Vol.24 No.4

In 2010, foliar symptoms were observed in the paprika leaves in Jinju city, Korea. Beet western yellows virus (BWYV) was identified in paprika by using the large-scale oligonucleotide chip assay. To investigate the occurrence of BWYV, a survey was performed on various crops, including paprika, from 2011 to 2014. Further, the presence of BWYV was consistently verified through literature survey from 2015 to 2017. BWYV infection has been identified in Solanaceae crops (bell pepper, hot pepper, and paprika), various weeds, and green peach aphids and it occurs on a nationwide scale. Cultivation using organic methods involved natural enemies and showed a high BWYV infection rate, which was more than that for conventional cultivation methods in greenhouse. The complete genome sequence of BWYV isolated from paprika was determined for the first time. The genome of the BWYV-Korea isolate consists of 5750 nucleotides and has six open reading frames. Sequence identity results showed maximum similarity between the BWYV-Korea isolate and the BWYV LS isolate (identity > 90%). This study is the first report of BWYV infecting paprika in Korea. The survey revealed that BWYV is naturalized in the domestic ecology of Korea.