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가감좌귀음(加減左歸飮)의 알레르기성 기관지천식(氣管支喘息) 반응(反應) 조절(調節) 효과(效果)
박은정,이해자,박종익,박영주,Park, Eun-Jung,Lee, Hai-Ja,Park, Jong-Ik,Park, Young-Joo 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2
Objective : KagamJwagwiEum(KJE) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of KJE, the author examined cytotoxicity and inflammatory cytokines secretion with human leukemic mast cell line, HMC-1. Methods: HMC-1 was stimulated with phorbol 12-myristate 13-acetate(PMA) and calcium ionophore A23187. KJE by itself had no effect on viability of HMC-l. The effects of KJE on the secretion of tumor necrosis $factor-alpha(TNF-{\alpha})$, interleukin(IL)-6 and IL-8 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Results : KJE inhibited PMA plus A23187-induced $TNF-{\alpha}$ and IL-6 secretion. But KJE had no effect IL-8 secretion: KJE had immunoregulatory effects on cytokines, increased secretion of NO and $TNF-{\alpha}$ but did not effect IL-12 secretion when the cells were primed and trigged with $IFN-{\gamma}$ in the peritoneal macrophages of C57BL/6 mice. Conclusions : Taken together, these results suggest that KJE inhibit the production of inflammatory cytokines in HMC-1 cells and activate macrophages.
박은정,Park, Eun Jung 한국가족자원경영학회 2019 가족자원경영과 정책 Vol.23 No.3
This study was aimed at exploring the differences in time use and time famine among college students ages 18 to 29, with a focus on gender and employment status, and to investigate the determinants of time famine. Data were from the 2014 Time Use Survey by the Korea National Statistical Office. Descriptive statistics, chi-square test, t-test, and logistic regression were used for the statistical analyses. The major findings are as follows. First, the employed spend significantly more time on work and less time studying than the unemployed, and females spend significantly more time on household work and less time on leisure than males. Second, employed students had higher levels of time famine than unemployed students. Third, irrespective of employment status, the education level of students' parents was identified as a determinant of time famine. Other determinants of time famine were age, job, work, and study time for employed students and gender, income, household work, and leisure time for unemployed students.
박은정,전길자,Park, Eun-Jeong,Jhon, Gil-Ja 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6
본 연구에서는 alloxan으로 유발시킨 당뇨병 쥐의 뇌에서 gangliosides 조성 변화를 조사하였다. 뇌 조직은 alloxan 주사후 3일째 희생시켜 얻었으며, gangliosides는 Folch-Suzuki 방법을 이용하여 추출하였다. Periodate-resorcinol 법으로 sialic acids를 정량한 결과, 당뇨병 쥐 뇌에서 sialic acid 양$(375{\mu}g)$이 정상$(783{\mu}g)$에 비해 반으로 감소하였다. 당뇨병 쥐 뇌에서 GT1b, GD1b, GD1a는 감소하였고 GM3와 band 1은 증가하였다. Sialidase처리로 band 1은 sialic acid가 없는 당지질임이 확인되었다. 2D-HPTLC로 당뇨병 쥐 뇌에서 GM1a와 GD1a 사이에 존재하는 마확인된 ganglioside가 증가함이 밝혀졌다. 미지의 ganglioside를 preparative TLC로 분리하여 ${\beta}-galactosidase$를 처리한 후 HPLC로 확인한 결과 Gal-GM1, Gal-Gal-GM1임이 확인되었다. The changes of ganglioside pattern from alloxan-induced diabetic rat brain, were investigated. Gangliosides were isolated by Folch-Suzuki method and analyzed by HPTLC. Sialic acid was determined by periodate-resorcinol method. The amount of gangliosides decreased 2 folds in diabetic brain. We found decreasing GT1b, GD1b, and GD1a, increasing GM3 and band 1, and no change of GM1a. In diabetic brain band 1 was identified as simple glycolipid with no sialic acid by the treatment of sialidase. The increase of unidentified ganglioside separated between GM1a and GD1a in diabetic brain found by 2-dimensional HPTLC. Unidentified ganglioside was collected by preparative HPTLC, treated with ${\beta}-galactosidase$ and analyzed by HPLC. As the result, this ganglioside revealed Gal-GM1 and Gal-Gal-GM1.
박은정,전길자 ( Eun Jeong Park,Gil Ja Jhon ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6
The changes of ganglioside pattern from alloxan-induced diabetic rat brain, were investigated. Gangliosides were isolated by Folch-Suzuki method and analyzed by HPTLC. Sialic acid was determined by periodate-resorcinol method. The amount of gangliosides decreased 2 folds in diabetic brain. We found decreasing GT1b, GD1b, and GD1a, increasing GM3 and band 1, and no change of GM1a. In diabetic brain band 1 was identified as simple glycolipid with no sialic acid by the treatment of sialidase. The increase of unidentified ganglioside separated between GM1a and GD1a in diabetic brain found by 2-dimensional HPTLC. Unidentified ganglioside was collected by preparative HPTLC, treated with β-galactosidase and analyzed by HPLC. As the result, this ganglioside revealed Gal-GM1 and Gal-Gal-GM1.
생약으로부터 세포분화유도물질의 검색 및 분리 및 분리 (I)
박은정,김진웅,Park, Eun-Jung,Kim, Jin-Woong 한국생약학회 1997 생약학회지 Vol.28 No.4
300 extracts derived from 100 plants were tested for their potential to induce HL-60 cell differentiation using NBT assay and NSE/SE staining methods. Morphological changes from suspended to adherent state of the cells were also observed by microscopic examination. In result, 55 extracts induced cell differentiation into monocyte/macrophage lineage in the NBT and the NSE assay.
토마토 과실에서 Superoxide Dismutase를 고발현하는 형질전환 식물체
박은정,이행순,권석윤,최관삼,곽상수,Park, Eun-Jeong,Lee, Haeng-Soon,Kwon, Suk-Yoon,Choi, Kwan-Sam,Kwak, Sang-Soo 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1
Superoxide dismutase (SOD)를 과실에서 고발현시킨 형질전환 토마토 (서광과 꼬꼬)를 개발하였다. 카사바 배양세포에서 분리한 CuZnSOD (mSOD1)를 과실에 우세적으로 발현하는 ascorbate oxidase promoter (ASOp)를 이용하여 ASOp :: mSOD1/pBI101 벡터를 제작한 후 Agrobacterium 매개로 자엽 절편체를 형질전환하였다. Kanamycin 저항성 식물체를 기관발생 경로로 재분화시킨 후 Southern 분석으로 형질전환을 확인하였다. 서광과 꼬꼬 토마토의 형질전환체와 대조구 식물체의 과실을 성숙 단계별로 분류하여 단백질 함량과 SOD 비활성도 (units/mg protein)를 측정한 결과, 단백질 함량은 열매가 익은 단계로 갈수록 점점 감소하여 완전히 익은 단계에서 가장 낮았다. SOD 비활성도는 형질전환 토마토의 열매의 모든 단계에서 대조구보다 높았으며 완전히 성숙한 과실에서 가장 높았다. 성숙한 형질전환 서광과 꼬꼬 과실에서 SOD 비활성도는 비형질전환의 것보다 각각 약 1.6배와 약 2.2배 높았다. SOD isoenzyme gel 분석에서 도입한 mSOD1로 추정되는 CuZnSOD 밴드가 형질전환체에서 과실 성숙에 따라 강하게 발현되었다. 이상의 결과로서 ASO promoter에 의해 SOD 유전자가 토마토 과실에 특이적으로 발현됨이 확인되었다. Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in plants. We have developed transgenic tomato plants overexpressing a cassava SOD in fruits. Three transgenic tomato plants (one from cv. Pink forcer and two from cv. Koko) using a new vector system, ASOp :: . mSOD1/pBI101, harboring ascorbate oxidase promoter (ASOp) expressing dominantly in cucumber fruits, CuZnSOD cDNA (mSOD1) isolated from cultured cells of cassava, and nptll gene as a selectable marker were successfully developed. SOD specific activity (units/mg protein) in transgenic fruits of both cultivars was increased with maturation of the fruits. SOD specific activity of well-mature fruits in transgenic Pink forcer and Koko showed approximately 1.6 and 2.2 times higher than control fruits, respectively. The strength of SOD isoenzyme bands well reflected the SOD activity during the fruit maturation. These results suggested that SOD gene was properly introduced into tomato fruits in a fruit-dominant expression manner by ASO promoter.