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박용복,정남호,김홍석,최명숙 ( Yong Bok Park,Nam Ho Jeoung,Hong Seok Kim,Myung Sook Choi ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
Cholesteryl ester (CE) transfer between lipoproteins was studied in vitro in various incubation mixtures containing isolated lipoproteins and an inhibitor of lecithin: cholesterol acyltransferase (LCAT). Isolated high-density lipoproteins (HDL) exhibited a significant amount of cholesteryl ester transfer activity. In incubations of HDL with very low-density lipoproteins (VLDL) at 37℃, decrease of free cholesterol (FC) in human VLDL was mainly associated with decrease of cholesteryl ester (CE) in HDL. In this system, FC of human VLDL was transferred to human HDL, but could not be esterified due to LCAT inhibitor resulting in an accumulation of FC in HDL fraction. However, CE in human HDL was transferred to human VLDL during the incubation. This appears to be the major mean for keeping cholesterol equilibrium between lipoproteins. Another results support that cholesteryl ester transfer protein (CETP) promotes both the exchange and net transfer of cholesterol species between lipoproteins and that the net transfer proceeds in the direction of HDL to lower density lipoproteins, which are well agreed with the previous reports. In efforts to simplify the CETP assay method, cholesteryl-[^(14)C]-oleate in reconstituted HDL (R_(HDL)) covalently bound to agarose beads was used as a CE donor. This simple evaluation of CE transfer activity did not require the time-consuming ultracentrifugation before and after the incubation, that has been used in the previous assay. It also eliminates the use of heparine-MnC1₂ or dextran sulfate-MgC1₂ for the precipitation of LDL or VLDL, that is relatively costly.