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박승택,윤향석,형근영,조정구,이강창,김원신,김형민,전병훈,윤용갑,Park Seung-Taeck,Yoon Hyang-Suk,Hyoung Keon-Young,Cho Chung-Gu,Lee Kang-Chang,Kim Won-Shin,Kim Hyung-Min,Jeon Byung-Hoon,Yun Young-Gap 대한한의학방제학회 1999 大韓韓醫學方劑學會誌 Vol.7 No.1
In order to eludidate the mechanism of oxidative stress in cultured spinal motor neurons damaged by oxygen free radicals, cytoxicity was assesed by MTT assay and NR assay after spinal motor neurons from mouse were cultured in media containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, neuroprotective effects of several herb extracts on oxidant-induced neurotoxicity were examined in these cultures, compared with nerve growth factors such as basic fibroblast growth factor(bFGF). XO/HX decreased cell viability in dose- and time dependent manners on cultured mouse spinal motor neurons, and MTT50 and NR50 values were measured at 20mU/ml XO and 0.1mM HX for 3 hours in these cultures. bFGF significantlt increased cell viability. In neuroprotective of herb extracts, Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) was very effective in the prevention of the neurotoxicity induced by XO/HX in cultured mouse spinal motor neurons. From the above results, it is suggested that XO/HX shows toxic effect in cultured mouse spinal motor neurons and selective herb extracts such as Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) were very effective in the increase of cell viability against the neurotoxicity induced by oxygen radicals in these cultures.
Glucose Oxidase에 의(依)하여 손상(損傷)된 배양척수감각신경절세포(培養脊髓感覺神經節細胞)에 대(對)한 음양곽(淫羊藿)의 효과(效果)
박승택,이호섭,윤용갑,박병림,Park Seung-Taeck,Lee Ho-Sub,Yun Yong-Gap,Park Byung-Rim 대한한의학방제학회 1999 大韓韓醫學方劑學會誌 Vol.7 No.1
척수감각신경절세포에 대한 산소자유기의 신경독성효과에 대한 기전을 규명하기 위하여 여러 농도의 Glucose Oxidase(GO)를 배양 척수 감각신경절세포에 처리한 후 GO의 독성효과를 분석하였으며 또한 GO에 의하여 유발된 신경독성에 대한 음양곽(Epimedium Koreanum Nakai)의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. GO는 신경세포에 처리한 농도와 시간에 비례하여 세포의 생존율을 유의하게 감소시켰으며, 또한 음양곽이 GO의 독성효과를 효과적으로 방어하였다. 이상의 결과로부터 산소자유기인 GO는 생쥐의 배양 척수감각신경절세포에 독성을 나타냈으며 음양곽과 같은 한약추출물이 GO의 독성을 방어하는데 효과적인 것으로 나타났다. To evaluate the neurotoxic effect of oxygen radicals in cultured mouse spinal dorsal root ganglion(DRG) neurons, cytotoxicity was determined by MTT assay after cultured DRG neurons were grown in the media containing various concentrations of glucose oxidase(GO). In addition, neuroprotective effect of herb extract, Epimedium Koreanum Nakai was examined by MTT assay in cultured DRG neurons. Cell viability of cultured DRG neurons was remarkably decreased by GO in dose- and time-dependent manner, and Epimedium Koreanum Nakai protected remarkably GO-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured mouse DRG neurons, and herb extracts such as Epimedium Koreanum Nakai are effective in prevention of the neurotoxicity induced by oxygen radicals in cultured mouse DRG neurons.
회소돌기아교세포에 있어서 메틸수은에 의해 유도된 신경독성에 대한 glutathione의 영향
박승택(Seung Taeck Park),오재민(Jae Mm Oh),최민규(Mm Kyo ChOl),김정중(Jung Joong KIm),윤향석(Hyang Suk Yoon),정진원(Hyang Suk Yoon),박옥규(Jm Won Chung),전병훈(Ick Kyu Park),김원신(Byung Hun Jeon),정연태(Won SIn KIm) 대한체질인류학회 1997 해부·생물인류학 (Anat Biol Anthropol) Vol.10 No.1
에틸수은 (methylmecury, MM) 이 소의 배양 희소돌기아교세포에 미치는 신경독성효과를 규명하기 위하여 여러 농도의 methylmercuric chloride (MMC)가 들어있는 배양액에서 신경세포를 24시간 동안 배양한 다음 이에 대한 신경특성을 MTT 분석법에 의하여 조사하였으며 또한 MMC에 의한 옥성효과에 대하여 항산화제의 방어효과를 분석 하였다. MMC는 처리한 농도와 시간에 비례하여 소의 배양 희소돌기아교세포의 생존율을 강소시켰으며 glutathione이 MMC에 의해 유도된 신경독성을 방어하였다. 이상의 경파로 부터 MM은 소의 배양 희소돌기아교세포에 독성을 나타냈으며 glutathione과 같은 선택적인 항산화제가 MM에 의한 신경독성을 방어하는데 매우 효과적인 것으로 나타났다.
보문 : 배양 인체섬유모세포에서 산화염모제 성분인 Hydrogen Peroxide의 세포독성에 대한 까마중 추출물의 항산화 방어효과
박승택 ( Seung Taeck Park ),김명섭 ( Myung Seoup Kim ),서영미 ( Young Mi Seo ),김란 ( Ran Kim ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2012 대한미용학회지 Vol.8 No.3
To clarify the cytotoxicity and antioxidative effect of Solanum nigrum L. (SN) extract, cell adhesion activity and cell viability were analysed by XTT or MTT assay after human dermal fibroblasts (Detroit 551) were cultured in media containing various concentrations of H2O2. And also, the effect of antioxidant, butylated hydroxytoluene (BHT), against H2O2-induced cytotoxicity was examined. For the protective effect of SN extract on H2O2-induced cytotoxicity, Detroit 551 cells were pretreated with 40 or 60 μg/mL of SN extract for 2 hours. And also, the antioxidative effects of SN extract were measured by superoxide dismutase (SOD)-like activity and the inhibitory activity of lipid peroxidation. In this study, H2O2 remarkably decreased cell viability as well as cell adhesion activity in dose-dependent manner compared with control. And the XTT50 and MTT50 values were determined at 35 and 40 μM of H2O2, respectively. In the effect of antioxidant, BHT effectively prevented H2O2-induced cytotoxicity by the significant increase in cell adhesion activity. In the protective effect of SN extract on H2O2-induced cytotoxicity, SN extract remarkably increased cell adhesion activity which was decreased by H2O2-induced cytotoxicity, and also it showed both of SOD-like activity and the inhibitory activity of lipid peroxidation. From these results, it is suggested that the SN extract effectively prevented the cytotoxicity induced by an oxidant, H2O2 as component of oxidized hair dye via its antioxidative effect, and also it may be a putative resources for beauty as an alternative antioxidant in hair dye.
배양 흑색종세포에 있어서 NiSO₄의 세포독성 및 멜라닌화에 대한 감잎 추출물의 영향
박승택(Seung Taeck Park),이경완(Gyoung Wan Lee),서영미(Young Mi Seo) 인간식물환경학회 2014 인간식물환경학회지 Vol.17 No.2
To evaluate the effect of persimmon(Diospyros kaki Thunb.) leaves(PL) extract on the cytotoxicity and melanogenesis of nickel sulfate(NiSO₄), an environmental pollutant or an allergic contact dermatitis inducer, the colorimetric assay was performed to measure the cell viability and lactate dehydrogenase(LDH) activity as well as the tyrosinase activity and total amount of melanin in cultured B16/F10 melanoma cells. In this study, NiSO₄ significantly decreased cell viability in dose-dependent manner, and XTT₅₀ value was calculated at 106.7μM of NiSO₄. The cytotoxicity of NiSO₄ was revealed to be mid-toxic in these cultures by Borenfreund and Puerner’s toxic criteria. And also, vitamin E effectively prevented NiSO₄-induced cytotoxicity by the significant increase of cell viability. PL extract showed the antioxidative activity by the significant increase of cell viability and the decrease of LDH activity compared to positive control(NiSO₄). In the melanogenesis, PL extract significantly decreased both tyrosinase activity and total amount of melanin which were increased by NiSO₄-induced cytotoxicity. From these findings, it is suggested that the cytotoxicity of NiSO₄ is involved in oxidative stress, and PL extract effectively prevented the cytotoxicity and the melanogenesis induced by NiSO₄. Conclusively, natural component such as PL extract may be a putative resources for beauty or detoxification by the prevention of the toxicity and the melanogenesis of heavy metals related with oxidative stress such as NiSO₄.
산소자유기에 의해 손상된 배양 희소돌기아교세포에 대한 Iron-chelator의 영향
박승택(Seung Taeck Park),문연자(Yeun Ja Mun),오재민(Jae Min Oh),김정중(Jung Joong Kim),최민규(Min Kyu Choi),심재한(Jae Han Shim),임계택(Kye Taek Lim),정연태(Yeun Tai Chung) 대한체질인류학회 1996 해부·생물인류학 (Anat Biol Anthropol) Vol.9 No.2
간추림 산소자유기의 신경옥성에 대한 기전을 규명하기 위하여 소의 애양 희소돌기아교세포를 산소자유기에 노출시킨 뒤 이의 독성효과를 조사하고 또한 산소자유기에 의하여 유도된 신경독성에 대한 Iron-chelator의 방어효과를 MTT 분석법에 의하여 알아보았다. 배양 희소돌기아교세포를 20mU/ml XO 와 0.lmM HX가 혼합된 배양액에서 1~8 시간 동안 배양한 결과 산소자유기는 시간에 비례하여 세포의 생존율을 현저하게 감소시켰다. 또한 산소자유기에 의하여 유도 원 신경독성에 대하여 iron-chelator인 tetrakis (2-pyridylmethyl)ethylenediamine (TPEN)은 유의하게 방어효과를 나타낸데 비하여 또 하나의 Iron-chelator 인 desferroxamine (DFX)은 유의한 방어효과를 나타내지 않았다. 이상의 결과로 부터 산소자유기는 소의 배양 희소돌기아교세포에 독성을 나타냈으며 TPEN 과 같은 선택적인 iron chelator 가 신경옥성을 방어한테 효과적인 것으로 나다났다.
산소자유기에 의해 저해된 배양 척수감각 신경절 세포에 대한 상피세포성장인자의 영향
박승택(Seung Taeck Park),김형룡(Hyung Ryong Kim),채한정(Han Jung Chae) 대한약학회 1997 약학회지 Vol.41 No.1
In order to elucidate the cytotoxic effect of oxygen radicals on cultured spinal dorsal root ganglion(DRG) neurons derived from mouse. the neurotoxic effect of oxygen radicals was examined after cultured DRG neurons were exposed to xanthine oxidase(XO) and hypoxanthine(HX)-oxygen radical generating system. In addition. neuroprotective effect of epidermal growth factor(EGF) against oxidant-induced neurotoxicity was also evaluated in these cultures. The results were, as follows: 1. Lethal concentration 50(LC50) was 35mU/ml XO and 0.1mM HX in cultured DRG neurons. 2. Oxygen radicals induced the morphological changes such as the decrease of cell number and loss of neurites in these cultures. 3. EGF increased the cell viability and neurofilament in neurons damaged by oxygen radicals. From above the results, it is suggested that oxygen radicals have a cytotoxic effect on cultured DRG neurons of neonatal mouse and selective neurotrophic factors such as EGF are, effective, in blocking the neurotoxicity induced by oxygen radicals in cultured spinal DRG neurons.
배양 인체 피부 흑색종세포에 대한 제라늄오일의 항산화 활성 및 멜라닌 합성에 미치는 영향
박승택 ( Seung Taeck Park ),유영월 ( Young Wall Ryu ),김선주 ( Sun Ju Kim ),이귀영 ( Gui Yeong Lee ),정다정 ( Da Jung Jung ),장병수 ( Byung Soo Chang ),범희주 ( Hee Ju Beom ),이화정 ( Hwa Jeong Lee ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.3
For the purpose of searching for whitening resources using essential oil of herb, the antioxidative activity and melanogenesis of Geranium (Palargonium graveolens) oil were examined in cultured human skin melanoma cells (SK-MEL-3). the cell viability, superoxide dismutase (SOD)-like activity, inhibitory activity of lipid peroxidation, tyrosinase activity and total amount of melanin were measured by colorimetric assay. For this experiment, SK-MEL-3 cells were treated with the media containing 25~55 μU/mL, glucose oxidase (GO) for 4 hours, and assessed for the cytotoxicity. GO significantly decreased cell viability compared with control and XTT50 value was determined at 45 μU/mL of GO. In the antioxidative activity of Geranium oil, it showed SOD-like activity and the inhibitory activity of lipid peroxidation. While, in the melanogenesis of Geranium oil, Geranium oil significantly decreased the tyrosinase activity and total amount of melanin which were increased by GO-induced cytotoxicity. From these results, it is suggested that Geranium oil was effective in the protection of GO-induced cytotoxicity by antioxidative activity and inhibitory effect of melanogenesis. Finally, an essential oil such as Geranium oil may be a putative agent for the improvement of whitening effect.
산소자유기에 의해서 유도된 신경독성에 대한 Iron-chelator의 영향에 관한 연구
박승택(PARK Seung Taeck) 대한체질인류학회 1995 대한체질인류학회지 Vol.8 No.2
산소자유기에 의한 신경독성에 대한 기전을 규명하기 위하여 배양 생쥐 적수 감각 신경절 세포에 효소 적으로 발생시킨 산소자유기를 노출시킨 뒤 산소자유기에 의하여 유도된 신경독성효과를 조사하고 또한 산소자유기에 의해 유발된 신경독성에 대한 iron-chelator의 방어효과를 MTT assay와 neurofilament enzymeimmuno assay(EIA)에 의하여 분석하였다. 배양 신경세포를 25mU/ml xanthine oxidase(XO)와 0.3mM hypoxanthine(HX)에 3시간 동안 노출시킨 결과 시간에 비례하여 유의한 세포수의 감소를 보였다. 또한 산소자유기에 의하여 중재된 신경독성에 대하여 iron-chelator인 TPEN은 유의하게 방어효과를 보인 반면 DFX는 뚜렷한 방어효과를 나타내지 않았다. 위의 결과로부터 산소자유기는 배양 생쥐 척수 감각신경절 세포에 독성을 보였고 또한 이같은 신경 독성에 절이 관여하고 있으며 TPEN과 같은 선택적인 iron-chelator가 신경 독성효과를 방어하는데 효과적인 것으로 나타났다.
보문: Chromium Trioxide로 유발된 세포손상에 대한 Lavender 오일의 항산화 효과
박승택 ( Seung Taeck Park ),유영월 ( Young Wall Ryu ),김선주 ( Sun Ju Kim ),김지원 ( Ji Won Kim ),서영미 ( Young Mi Seo ),박미순 ( Mi Soon Park ),김은주 ( Eun Ju Kim ),이화정 ( Hwa Jeong Lee ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.2
The cell viability by XTT assay, DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and lipid peroxidation were measured to evaluate the cell injury of chromium trioxide (CrO3) and the antioxidative effect of lavender (Lavendula angustifolia L.) oil on the cell injury induced by CrO3 in cultured C6 glioma cells. C6 glioma cells were then treated with the media containing 30~50 μM of CrO3 for 48 hours. The results of this study showed that CrO3 remarkably decreased cell viability in dose-dependent manner and the XTT50 value was appeared at 40.5 μM of CrO3. In the effect of antioxidant, SOD on CrO3-mediated cytotoxicity significantly increased the cell viability which was decreased by CrO3. Meanwhile, in the protective effect of lavender oil on the cytotoxicity induced by CrO3, it remarkably increased the cell viability damaged by CrO3-mediated cell injury and also showed the DPPH-radical scavenging activity, SOD-like activity and the inhibitory activity of lipid peroxidation. From these results, it is revealed that the cell injury by CrO3 was involved in oxidative stress and lavender oil was effective in the protection of CrO3-mediated cell injury by antioxidative effect.