인삼 (Panax ginseng C.A. Meyer)의 Microsatellite 마커에 대한 유전적 다형성과 특성 규명

박선화(Sun Wha Park),현영세(Young Se Hyun),정기화(Ki Wha Chung) 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.3

인삼에 대한 microsatellite 개발은 다른 분자적 마커들에 비해 늦게 이루어져, 최근에 와서야 인삼의 microsatellite 들이 보고되고 있는 실정이다. 본 연구에서는, 분리된 microsatellite들 중에서 5 개의 다형성 마커를 선별하여 국내 경작지나 시장에서 유통되는 인삼을 대상으로 유전적 다형성을 조사하고, 각 마커의 특성을 규명하였다. 유전자형 분석은 변성 PAGE와 silver staining법으로 하거나 형광표지 primer로 표지한 PCR 산물을 자동 염기서열 분석기로 분석하였다. 본 연구에서 개발한 5개의 microsatellite 마커들의 평균 대립유전자 수는 3.2 개였으며, 평균 GD는 0.367 였다. 전체적으로 볼 때, PG1419가 가장 높은 다형성을 보였으며 (PIC: 0.460, GD: 0.543), PG770은 가장 낮은 다형성을 나타내었다 (PIC: 0.070, GD: 0.078). 각 좌위들의 예상 이형 접합도 (Hexp)는 0.077에서 0.541 (mean = 0.313)로 계산되었으나, 관측 이형접합도 (Hobs)는 0.040에서 0.130 (mean = 0.083)으로 훨씬 낮게 관찰되었으며, 유전자형의 분포는 Hardy-Weinberg 평형상태에서 벗어남을 보였다. 본 연구에서 개발한 인삼의 microsatellite 마커들은 인삼의 분자적 마커의 데이터베이스 확립의 기초 자료로 활용될 뿐 아니라, 인삼의 분자적 구별법 및 QTL 좌위의 염색체지도 작성에 유용하게 활용될 것이다. Ginseng (Panax ginseng C.A. Meyer) is one of the most important medicinal plants in East Asia. Microsatellite or simple sequence repeat (SSR) markers are used in obtaining genetic analysis and authentication in many plants. The present study examined five microsatellites in conjunction with P. ginseng in Korea. The total observed allelic number was 17 (mean = 3.4), and gene diversities varied from 0.078 to 0.543 with an average of 0.314. Through a combined analysis of five loci in 100 ginseng samples, 44 different combined genotypes were observed. Expected and observed heterozygosites ranged from 0.077 to 0.541 (mean = 0.313) and 0.040 to 0.130 (0.083), respectively. All examined loci exhibited deficiency of heterozygosity and deviation from the Hardy-Weinberg equilibrium. Such results may be explained by the non-random mating and inbreeding that has occurred for several hundred years. These microsatellite markers could be used for the study of molecular genetics and the establishment of DNA marker database, as well as authentication of ginseng species and chromosomal mapping of QTL loci in P. ginseng.

법과학 현장시료에서 Yfiler^® PLUS PCR Amplification Kit의 민감도 연구

정주연,김경숙,박선화,임시근,이동섭,이양한,Jung, Ju Yeon,Kim, Kyoung Sook,Park, Sun Wha,Lim, Si Keun,Lee, Dong Sub,Lee, Yang Han 한국분석과학회 2016 분석과학 Vol.29 No.1

A variety of Y-STR analysis kits have been developed and used in the forensic field. Prior to the forensic application of a new kit, laboratory validation and sensitivity tests are essential processes in selecting suitable alternatives and for assuring that standard operating procedures are followed. In this paper, we have performed a sensitivity study of a new commercial kit, the Yfiler^® PLUS PCR Amplification Kit (Yfiler plus kit, released in 2014) by comparing it with the AmpF/STR^® Yfiler^TM PCR Amplification Kit (Yfiler kit, released in 2004). The Yfiler plus kit includes the 17 Y-STR loci of the Yfiler kit and has been supplemented with 10 new Y-STR loci. First, we analyzed the sensitivity difference between the two kits using commercial control DNA 2800M and 007. In addition, we compared the detection rate between the two kits from the 16 selected forensic casework samples of less than 0.5 ng concentrations. The results show that the sensitivity and detection rate of the Yfiler plus kit are higher than the corresponding rates of the Yfiler kit. In addition, we were able to obtain more Y-STR profiles with the use of the new kit. Thus, we suggest that Yfiler plus kit is a more effective forensic tool to detect Y-STR profiles from forensic casework samples of low concentrations. 법과학 분야에서 다양한 Y-STR 분석 키트가 개발되어 사용되고 있고, 새로운 키트의 법과학적 적용에 앞서 DNA 감정에 적절한 분석 키트들의 선정과 표준작업절차서의 작성을 위해 실험실 내의 내부적 유효성 검증 및 민감도 시험은 필수적인 과정이다. 본 논문에서는 새로운 상업용 키트인 Yfiler^® PLUS PCR Amplification Kit (Yfiler plus 키트, 2014년 출시)를 AmpF/STR^® Yfiler^TM PCR Amplification Kit (Yfiler 키트, 2004년 출시)와 비교함으로써 민감도에 대한 연구를 수행하였다. Yfiler plus 키트는 Yfiler 키트의 17 개 Y-STR 좌위를 포함하면서 새로운 10 개의 Y-STR 좌위가 추가되었다. 먼저, 표준 DNA 시료인 2800M, 007을 이용하여 두 키트 간의 민감도 차이를 분석하였고, 선별된 0.5 ng 미만의 법과학 현장시료 16 개로부터 검출률을 비교하였다. 그 결과, Yfiler 키트보다 Yfiler plus 키트가 높은 민감도와 검출률을 보였고, 더 많은 좌위에서 Y-STR 프로필을 얻을 수 있었다. 이러한 결과들로부터 낮은 농도의 법과학 현장시료에서 Yfiler plus 키트가 Y-STR 프로필을 검출하는데 더욱 효과적인 분석키트임이 확인되었다.

Sulfisomidine배합 산성고자당액상배지검사의 우식예측타당도에 관한 종단 연구: 3년 후 결과

나자영 ( Ja-young Na ),박선화 ( Sun-wha Park ),주현정 ( Hyun-jeong Ju ),김인자 ( In-ja Kim ),이흥수 ( Heung-soo Lee ) 대한구강보건학회 2016 大韓口腔保健學會誌 Vol.40 No.3

Objectives: The purpose of this study was to evaluate the prediction validity of the caries activity test with a sulfisomidine mixture (SAHS test). Methods: This longitudinal follow-up study was conducted for 3 years. The subjects were 155 elementary schoolchildren. Oral examination was performed by examining each tooth surface of the subjects. The number of teeth with new caries lesions was calculated by comparing between the baseline data of the initial oral examination and the results of the second oral examination performed after 3 years. The Dentocult SM test was used as the reference in the analysis of the caries prediction validity of the SAHS test. The items of the validity test for carries prediction were as follows: sensitivity, specificity, predictive value, and likelihood ratio Results: The correlation between new caries lesions and the SAHS test scores was greater than that between new caries lesions and the Dentocult SM test scores. The receiver-operating analysis revealed that the area under the curve of the SAHS test was higher than that of the Dentocult SM test. The caries prediction validity of the SAHS test (grade 12) was as follows: sensitivity, 0.71-0.70; specificity, 0.60-0.58; positive predictive value, 0.79-0.78; negative predictive value, 0.49 (screening criterion 5). The SAHS test scores were similar to or higher than the scores in the items of the Dentocult SM test. Conclusions: The SAHS test is considered useful for clinical applications.

정신분열병과 기분장애 환자의 언어적 기억능력과 기억과정의 특성에 대한 연구

이소연,이분희,이정애,김계현,김용구,박선화,Lee, So-Youn,Lee, Bun-Hee,Lee, Jung-Ae,Kim, Kye-Hyun,Kim, Yong-Ku,Park, Sun-Wha 대한생물정신의학회 2005 생물정신의학 Vol.12 No.2

Objectives:This study was to compare verbal memory ability among patients with schizophrenia, bipolar manic patients and unipolar depressive patients, and to understand their charicteristics of memory process. Methods:All subjects were hospitalized patients and had been interviewed by using the Structured Clinical Interview for DSM-IV(SCID). Schizophrenic patients(N=40), bipolar manic patients(N=17), and unipolar depressive patients(N=20) were assessed with K-AVLT for verbal memory and with K-WAIS for verbal IQ. Three groups were compared regarding total immediate recall, delayed recall, delayed recognition, learning curve, memory retention, and retrieval efficiency under controlled verbal IQ. Multiple regression analysis was performed to find which clinical factors have an influence on verbal memory ability. Results:In MANCOVA, differences of verbal memory test scores among the groups were statistically significant(F=1.800, p<.05). In post hoc analysis, Patients with schizophrenia and bipolar mania showed poorer performance in immediate recall, delayed recall, delayed recognition, retrieval efficiency than unipolar depres- sive patients. And schizophrenics performed poorly in delayed recall, delayed recognition, retrieval efficiency than nonpsychotic affective disorder group, but no difference in total immediate recall, delayed recall, delayed recognition, retrieval efficiency between the schizophrenic group and the psychotic affective group. Conclusions:These results partially confirm previous reports of verbal memory ability among major psychiatric disorders. Our results showed that psychotic symptoms were related with verbal memory, and longer duration of illness was related with poorer performance in schizophrenia and unipolar depression.

가열에 의한 지하수 중 <SUP>222</SUP>Rn 제거율 고찰

김문수(MoonSu Kim),김현구(Hyun-Koo Kim),박선화(Sun-Wha Park),김형섭(Hyoung-Seop Kim),주병규(Byoung-Kyu Ju),김동수(Dong-Su Kim),조성진(Sung-Jin Cho),양재하(Jae-Ha Yang),권오상(Oh-Sang Kwon),김태승(Tae-Seung Kim) 한국분석과학회 2015 분석과학 Vol.28 No.5

법과학 현장시료에서 Yfiler<SUP>®</SUP> PLUS PCR Amplification Kit의 민감도 연구

정주연(Ju Yeon Jung),김경숙(Kyoung Sook Kim),박선화(Sun Wha Park),임시근(Si Keun Lim),이동섭(Dong Sub Lee),이양한(Yang Han Lee) 한국분석과학회 2016 분석과학 Vol.29 No.1

四肢發生 過程에 關한 觀察

全容赫,朴善華 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.2

Developmental sequence of events of fore-limb in DDY mouse embryo were observed to obtain the data on shape ; on histology with special reference to the apical ectodermal ridge(AER), programmed cell death(PCD) and chondro-osteogenesis and ; on changes of glycoconjugates. Fore-limb buds appeared between the day 9.5 and 10 of gestation(plug day=0) and distal ends grew into paddle-like plates from day 11, plates condensed to form digital rays on day 12, webbed fingers on day 13 and fingers were separated on day 14. Well developed AER appeared with the beginning of elevation of buds and decreased in size from day 11 and remained remnants on day 12.5 of gestation. Proximal core mesenchyme began to condense form day 11 and transformed to precartilaginous zone 12 hours later chondrified from day 12.5 and ossified from day 15 and these phenomena proceeded proximo-distal sequence. Programmed cell death happened only between the day 10.5 to 11.5 and were confined to the proximal and middle zone of mesenchyme, which comes into question how PCD In motioned area and time correlate with subsequent morphogenses. PAS positive reactivity was gradually increased in the epithelium. AER and cartilaginous zone with the development proceed but that of mesenchyme was weak throughout the period. and in comparison with PAS reactivity, PAPS reactivity to the epithelium was always less but no difference in cartilaginous zone which suggest that amount and component of glycoeonjugates are variable and tissue/stage-specific in the developing limb.

二次 口蓋組織 發生時 植物凝集素 表現에 關한 組織化學的 觀察

崔崇仁,朴善華,全容赫 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.1

Carbohydrate rich macromolecules which have been demonstrated on the cell surface of a wide variety of animal cells have been implicated in behavior of the living cell including intercellular communication, the regulation of cell growth and differentiation, immune response, cellular recognition and adhesion during embryonic development. They also have been played an important role in secondary palatogenesis of developing mammalian fetuses but still little have been reported on their carbohydrate residues in detail yet. While, lectins have been known to combine specifically with sugar residues of such macromolecules on cell surfaces and hence bind cell together. Therefore, the component of carbohydrate residues concerned with palatogenesis and their changes according to the process of palatal formation were investigated in DDY mouse using commercially available lectins, such as Concanavalin A (Con A), Ricinus communis agglutinin (RCA), wheat germ agglutinin (WGA), soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA) , peanut agglutinin (PNA), Pinellia ternata agglutinin (PTA) and Ulex europaeus agglutinin (UEA) by application of biotin-avidin peroxidase and benzidine substrate. The results obtained are as follows: 1. Early period in palatal shelf formation, none but RCA showed light positive reaction to palatal epithelia and mesenchyme. 2 . Just prior to shelf elevation and reorientation, RCA and WGA revealed strong positive reaction to palatal tissue especially RCA on epithelia and WGA on mesenchyme, While Con A reacted lightly with both tissues. 3. Just prior to fusion after elevation, Con A showed strong positive reaction to epithelia especially on medial edge epithelia, whereas the positive reactivity with RCA and WGA was markedly reduced in both tissues. 4. The positive reactivity with Con A was rapidly decreased after palatal fusion. 5. SBA, DBA, PNA, PTA and UEA showed almost negative reaction to the developing palatal tissue through the palatogenesis. With the results, it may be considered that macromolecules containing D-galactosyl residues and sialic acid could be related to the palatal shelf elevation, while materials known to appear in palatal shelf epithelia just prior to fusion would be considered as α- D-mannosyl and α-D-glucosyl residues containing macromolecules.