구기자가 흰쥐의 Collagen 합성에 미치는 효과

박미화,김미향 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-

모든 여성들은 폐경기를 전 후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적으로 폐경을 유발시킬 수 있는 난소 절제 쥐에서의 골손실의 무를 결합조직 중의 collagen함량 변화를 통하여 그 효과를 검토하였다. 난소 절제 후 구기자 추출물 투여에 의해 골·연골조직과 피부조직에서 collagen함량이 난소를 절제하지 않은 SHAM군에 비해서도 증가하는 경향을 보였고, 구기자 추출물 투여군 모두에서 collagen 합성 량이 유의적으로 증가하였다. 구기자 추출물이 폐경에 의해 estrogen감소로 생기는 collagen합성 저하를 회복시킨 본 실험결과로 미루어 보아 구기자 중의 phytoestrogen이 estrogen 유사 효과를 나타냄을 알 수 있었고, 외 인성 estrogen 투여로 인한 부작용을 줄여 줄 수 있을 것으로 기대된다. 이상과 같이 폐경기 여성에게 구기자 추출물의 섭취는 폐경으로 인한 장애를 감소시키기 위한 장애를 감소시키기 위하여 외 인성 estrogen의 장기 복용에 의한 부작용을 구기자 중의 phytoestrogen이 대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요한 것으로 생각된다. The aim of this study was to evaluate the effect of Lycium chinense Miller (LC) on the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rat (SHAM), ovariectomized control rat (OVX-CONTROL), and ovariectomized rats supplemented with the LC 50mg/kg bw/day (OVX-LC50). The LC ethanol extract were orally administrated 1ml per day. The OVX rat were significantly heavier than the sham-operated rat at all time points, but supplementation with the LC extracts tended to gain weight less than OVX-CONTROL. Supplementation with the LC extracts prevented a decrease in the collagen level in bone and cartilage tissues. These results are consistent with the conclusions based on the estrogenic activities of LC. Therefore, it may be used to possibly improve the quality of life in menopausal women.

감염공막염의 임상양상과 치료 결과

박미화,유웅선,김현아,정인영,서성욱,김성재 대한안과학회 2019 대한안과학회지 Vol.60 No.6

목적: 감염공막염의 위험인자, 임상양상, 원인균 및 치료 경과와 시력 예후에 대해서 보고하고자 한다. 대상과 방법: 2011년 1월부터 2018년 12월까지 본원 안과에서 입원 치료를 받은 감염공막염환자 중에서 3개월 이상 경과관찰이 가능하였던 20명을 대상으로 의무기록을 후향적으로 분석하였다. 감염공막염의 위험인자, 임상양상, 동정된 원인균, 치료 경과와 예후 등을 조사하였다. 결과: 환자들의 평균 나이는 69.2 ± 8.4세였고, 평균 입원 기간은 11.3 ± 5.8일이었으며, 증상이 나타나고 입원하기까지의 시간은 16.8 ± 13.9일, 그리고 평균 경과관찰기간은 23.3 ± 25.4개월이었다. 모든 환자들은 익상편수술의 과거력이 있었으며, 배양검사 결과 양성이 나온 환자는 18명(90%)이었고, Pseudomonas aeruginosa가 12명에서 동정되었다. 입원 당시에 수술을 시행한 경우는 18명(90%)이었고, 입원에서 수술을 시행하기까지의 시간은 평균 4.1 ± 4.4일이 걸렸다. 결론: 감염공막염의 위험인자로는 익상편수술의 과거력이 있었으며, 대개가 녹농균의 감염에 의해 발생하였다. 정확한 원인균을 파악하고 그에 따른 적절한 항생제 치료와 수술적 치료를 병행하는 것이 시력 예후를 향상시킬 수 있는 방법으로 생각된다. Purpose: To investigate risk factors, clinical features, pathogenic organisms, and outcomes in patients with infectious scleritis. Methods: This study was a retrospective review of 20 patients with infectious scleritis who were admitted from January 2011 to December 2018 in a single tertiary hospital, with at least 3 months of follow-up. We analyzed age, risk factors, clinical manifestations, pathogenic organisms, treatment, and outcomes of infectious scleritis. Results: The mean patient age was 69.2 ± 8.4 years and the mean duration of hospitalization was 11.3 ± 5.8 days. Furthermore, the mean duration of symptoms before presentation was 16.8 ± 13.9 days; patients were followed for a mean duration of 23.3 ± 25.4 months. All patients had prior pterygium surgery. Eighteen patients (90%) were culture-positive and Pseudomonas aeruginosa (P. aeruginosa) was identified in 12 eyes. In the acute stages, adjuvant surgical intervention was performed for 18 patients (90%) for these patients, the mean duration of hospitalization before surgery was 4.1 ± 4.4 days. Conclusions: The most common risk factor and pathogenic organism for infectious scleritis were prior pterygium surgery and P. aeruginosa, respectively. Identification of specific causative organisms and corresponding antibiotic treatment with adjuvant surgical intervention may improve visual prognosis in patients with infectious scleritis.

Bacillus subtilis BK-17이 난소를 절제한 흰쥐의 혈 중 지질 함량 변화에 미치는 영향

박미화,김미향 신라대학교 자연과학연구소 2006 自然科學論文集 Vol.15 No.-

여성은 폐경 시 estrogen에 의해 정상여성보다 심혈관련 질환의 발병률이 높아진다. 따라서 본 연구에서는 혈관관련 질환의 예방에 사용되는 혈전용해제 개발의 목적의 일환으로 갱년기 장애 시 유발되는 체내의 지질 함량 증가에 있어서 Bacillus subtilis BK-17이 미치는 영향을 조사하기 위해 흰쥐에 인위적 폐경을 유도하여 Bacillus subtilis BK-17를 투여함으로써 그에 따른 혈 중 지질 함량에 미치는 영향을 검토하였다. 그 결과 난소절제 후 Bacillus subtilis BK-17추출물 투여는 난소 절제에 의해 야기되는 total-cholesterol 의 증가를 감소시키고, 중성지질을 감소시키며, HDL-cholesterol의 감소를 증가시키는결과, Bacillus subtilis BK-17이 갱년기 장애 시 발생찰 수 있는 지질변화에 효과가 있는 것으로 나타나 혈전 용해제로서의 기능이 있는 것으로 사료되어 진다. Bacillus subtilis BK-17, which was isolated from a decaying rice plant, known as fibrinolytic enzyme. The ovariectomized rat is proposed as an experimental model for the rapid development of menopausal symptoms. Following loss of ovarian function, postmenopausal women display an increased incidence of hypertension and coronary heart disease, which is at least partially attributable to an increase in the total cholesterol, LDL-cholesterol and triglyceride levels, as well as a reduction in the HDL-cholesterol levels. Two groups were surgically ovariectomized (OVX). The third group was sham operated. Sprague- Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-Control), ovariectomized rats supplemented with Bacillus subtilis BK-17 (OVX-BK17). The aim of this study was to evaluate the effects of the purified fibrinolytic enzyme from Bacillus subtilis BK-17 on the lipid on serum in ovariectomized rats.

노인전문병동에 입원한 뇌졸중 환자의 낙상위험요인

박미화,송경애 노인간호학회 2005 노인간호학회지 Vol.7 No.1

Purpose: To identify the risk factors of falling in stroke patients. Method: Data collection was performed from the 438 stroke patients' medical record using. A questionnaire developed by the authors to survey falling. Data were analyzed by SAS program, through which the frequency, percentage, mean, standard deviation, t-test and x^(2)-test, and multiple logistic regression analysis. Results: The falling rate of subjects during their stay at the hospital was 14.2%. The length of stay was longer and the morbidity duration of stroke was shorter in the falling group than in the non-falling group. The risk of falling was 6.4 times higher in those who had experienced falling within 1 year before admission, 3.4 times in those with depression, 2.9 times in those with irritability, 2.0 times in those with urinary dysfunction, 5.7 times in those who used a quad cane in walking, and 3.3 times in those who used a walker. Conclusion: The risk factors of falling in stroke patients admitted to geriatric hospital include experience in falling, accompanying diseases and symptoms, walking level and assistive device used in walking. It is necessary to analyze the causes of falling based on results of the present study and to develop positive nursing interventions to prevent falling.

일부 간호대학생의 자아존중감과 건강증진행위에 관한 연구

박미화 성인간호학회 1999 성인간호학회지 Vol.11 No.1

The purpose of this study was to investigate degree of the health promoting behavior and self-esteem of nursing college students, and to identify the relationship between self-esteem and the health promoting behavior. The sample consisted of 122 college nursing students and 100 university nursing students. The instruments for this study were structured questionnaire which included : health promoting behavior practice level (60items), self-esteem(10), sociodemographic characteristics(10). Data were collected from September 14 to September 28, 1998 and analyzed by SAS program t-test, ANOVA, Pearson correlation coefficient, and multiple regression. The results of this study are as follows : 1. The results of the comparison between the two group were not significantly different for self-esteem and health promoting behavior and socio-demographic characteristics. The average score for the health promoting behavior was high 162.5(total 240), and self-esteem was high, too 30.5(total 40). In the subcategories the highest practice level was "personal hygiene(3.30)", and the lowest level was "health management(1.57)". 2. Health promoting behavior was significantly correlated with self-esteem(r=.4970, p=.0001). 3. Self-esteem was the main factor predicting health promoting behavior and accounted for 24.69% among variance. 4. There was a statistically significant the difference on health promoting behavior according to "personality"(t=2.58, p=.0106), "residence pattern"(F=2.90, p=.0360) and on self-esteem according to "religion"(t=-2.20, p=.0291) and "personality"(t=4.40, p=.0001). In conclusion, there was not a significant difference between those of college nursing students and university nursing students on self-esteem and the health promoting behavior. Self-esteem correlated with health promoting behavior positively, and was identified to be an important variable that contributed to health promoting behavior.

오늘날 한국도예의 흐름

박미화 The Korea Foundation 2005 Koreana (Korean) Vol.19 No.2

장애학생 진로교육 역량에 대한 초등학교 교사의 실행수준 및 교육요구 분석

박미화 한국지체.중복.건강장애교육학회 2022 지체.중복.건강장애연구 Vol.65 No.3

Purpose: The purposes of this study were 1) to examine elementary school teachers’ perceptions of the importance of the career education competency of students with disabilities in elementary school, 2) to assess their level of implementation, and 3) to clarify the educational needs for improving career education. Method: A survey was conducted with elementary teachers in Gyeonggi-do. A total of 155 responses were collected and statistically analyzed using SPSS Statistics 26. Results: First, the elementary school teachers recognized the importance of the career education competency of students with disabilities more than the level of performance. Second, the implementation of the career education was the most significant factor for educational needs. The factor includes the implementation of creative, local, career-related field studies and activity programs as well as career counseling for the parents of students with disabilities. Third, the priority of educational needs depended on the teacher’s class, the experience of inclusive education, and teaching experience. Conclusion: To educate and support prioritized competencies based on educational needs, a variety of practical training programs should be developed.

동해안 심층수의 혈행 및 혈중지질 개선효과

박미화,배송자,김미향,Park, Mi-Hwa,Bae, Song-Ja,Kim, Mihyang 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.2

식생활의 서구화로 인하여 심혈관계질환의 발생빈도 증가로 인한 사망률이 늘어나고 있다. 따라서 최근 이용가치가 인정 되어지고 있는 해양 심층수를 이용하여 심혈관계 질환의 개선효과를 알아보았다. 심층수의 혈소판 응집 억제능을 알아보기 위해, in vitro 실험에서 washed platelet를 분리하여 심층수를 농도별로 처리한 후, collagen으로 혈소판 응집을 유도하여 심층수의 응집억제 실험을 한 결과 그 농도별로 응집이 감소되었다. 그리고 in vivo 실험에서도 심층수를 투여하지 않은 군에 비해 심층수를 투여한 군에서 혈소판 응집이 감소하였으므로 이는 심층수가 혈소판 응집에 효과적인 것으로 보여진다. 또한 혈청 중의 중성지질 함량이 감소되었고, 고밀도 지단백은 증가하였다. 혈류 속도 측정에서도 심층수를 투여한 그룹이 투여하지 않은 군에 비해 더 빠른 속도로 모세혈관을 빠져나가는 것을 볼 수 있었으며, 이것으로 보아 심층수를 투여한 그룹에서 혈행에 있어 좋은 효과가 있음을 나타내었다. 본 실험의 결과 심층수가 혈소판 응집, 혈청 지질 농도, 혈류 속도에 유효한 효과를 나타냄을 알 수 있었다. 이는 잘못된 식습관으로 오는 비만이나, 심혈관계질환에 유익한 결과를 줄 것으로 사료된다. The more the population grow, the severer the shortage of a basic human needs such as clean water, food, energy resources and so on. Recently, we aware that deep sea water can be utilized to solve comprehensively food, energy and water problems. In this study, inhibitory effect of the deep sea water of east coast (DSW) on platelet aggregation were investigated using washed platelet and beneficial effect of the change of lipid concentration on serum at various time intervals for three weeks. In this study six groups of 6-month-old Sparague-Dawley rats were examined. An intact group served as controls (C-1D : breeding for one day, C-1W : breeding for one week, C-3W : breeding for three week). The fourth group (1D-DSW) supplemented with DSW for one day. The fifth group (1W-DSW)supplemented with DSW for a week. The sixth group (3W-DSW)supplemented with DSW for three weeks. The total cholesterol and triglyceride concentration on serum of supplemented groups with the DSW for one or three weeks were significantly decreased. The serum HDL-cholesterol level in the DSW groups were significantly higher than the level in the control group. The supplementation of DSW for one day did not appear to have such a beneficial effect on lipid level. The ability of platelet aggregation of supplemented groups with DSW was less than control group. These results suggest that supplementation with DSW is positively influence on lipid concentration and platelet aggregation.

매생이 추출물이 난소를 절제한 흰쥐 결합조직의 collagen 함량에 미치는 영향

박미화,김미향,Park, Mi-Hwa,Kim, Mi-Hyang 한국생명과학회 2006 생명과학회지 Vol.16 No.7

여성의 폐경에 의한 estrogen의 감소는 골다공증을 유발시킨다고 알려져 있다. 최근 새로운 식품으로 각광받고 있는 매생이 추출물을 인위적 갱년기 장애를 유발시킨 흰쥐에 투여하여 혈 중 ALP활성 변화와 골 중의 collagen의 함량 및 collagen 가교물질 pyridinoline과 deoxypyridinoline 함량 변화를 측정하여 그 효과를 검토해 보았다. 매생이 추출물을 갱년기를 유도한 흰쥐에 투여하여 혈 중 ALP활성 변화에 미치는 영향을 검토한 결과 난소절제에 의하여 ALP활성이 증가하였으나, 매생이 추출물 투여한 군에서 ALP활성이 억제되는 것을 확인할 수 있었다. 또한 난소절제에 의해 연골의 collagen 함량이 감소되었는데, 이는 매생이 추출물을 투여함으로써 정상적인 수치로 증가하는 경향을 나타내어 매생이 추출물이 골 대사와 관련된 골 대사질환에 유효한 효과를 나타낼 것으로 추측된다. 또한 collagen 가교물질로써 골대사의 biomaker인 pyridinoline 및 deoxypyridinoline의 연골 중의 함량은 난소절제에 의해 감소하였고, 매생이 추출물 투여에 의해 증가하여 estrogen부족으로 인한 골 손실에 매생이 추출물이 유익한 효과를 가지는 것으로 보이며, 이는 매생이 중에 함유되어 있는 phytoestrogen에 의한 것으로 추측되어 앞으로 구체적인 검토가 필요한 것으로 사료된다. Osteoporosis is one of the major health problem affecting postmenopausal women. Estrogen deficiency results in an increase in bone turnover, lead to bone resorption and an increase risk of fracture. The aim of this study was to evaluate the effects of Capsosiphon fulvecense extract (SCF) on the collagen content of the connective tissues and alkaline phosphatase activity of serum in ovariectomized estrogen-deficient rats. Three groups were surgically ovariectomized (OVX). The fourth group was sham operated. Sprague-Dawley female rats were randomly assigned to the following groups : sham-operated rats (Sham), ovariectomized control rats (OVX-control), ovariectomized rats supplemented with CsF at 50mg/kg body wt (OVX-CSF50) and ovariectomized rats supplemented with CsF at 200mg/kg body wt (OVX-CSF200). The Capsosiphon fulvecense extracts were orally administrated at 1mL per day. The ovariectomy caused a decreasing in the levels of collagen content in bone, cartilage, skin and lung tissues. However CSF groups, supplementation with Capsosiphon fulvecense extract, were increased the level of collagen content in bone, cartilage, skin and lung tissues than OVX-control group. Alkaline phosphatase activity also were increased and calcium levels were decreased than OVX-control on serum. These results suggest that Capsosiphon fulvecense supplementation prevents postmenopausal bone loss, thus it may be used possibly to improve the quality of life in menopausal women.

통합교육을 위한 농업교사의 특수학생 지도능력과 교육요구

박미화 한국농·산업교육학회 2005 농업교육과 인적자원개발 Vol.37 No.4

The purpose of this study was to determine the educational needs of agricultural teachers on the competencies required for teaching exceptional students in vocational high schools. The subjects for the study were 418 agricultural teachers by the cluster sampling drawn from the random sampled 21 vocational agriculture high schools. The data was collected by using the mailed questionnaire. There was a response rate of 58%. First, agricultural teachers perceived all the thirteen competencies required for teaching exceptional students as highly important ones. Second, they have the high educational needs for in-service education on the thirteen competencies for teaching exceptional students in the special classes. Third, agriculture teachers have a different level of educational needs on the competencies according to their gender, years of teaching experience, terminal education level, path of entering teaching profession, teaching subject, classroom teacher, and position level.