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박래옥,김재훈,이성종,서주태,김재동,이연수,김병기,배석년 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.3
목적 : 난소암의 발생 위험인자를 토대로 배란 기간동안 인간 정장(human seminal plasma, hSP)에 노출이 감소되었을 경우 상피성 난소암이 증가할 수 있다는 가설을 검증하기 위해 난소암 세포주에 대한 정장의 효과를 실험하였다. 연구 방법 : 난소암 세포로 SK-OY-3, OYCAR-3을 이용하였고, 대조군으로 자궁경부암 세포인 Caski, 섬유모세포인 Wl-38을 사용하였다. 결과 ; MTT 정 량 실험에서 1:50으로 희석된 hSP는 난소암 세포 괴사를 유발하였으나 자궁경부암세포와 섬유모세포에서는 반응이 없었다. Flow cytometry, DNA laddering, 생화학적 정량 실험, 형태학적 관찰을 통하여, hSP는난소암 세포주에서 세포고사(apoptosis)보다는 세포괴사(necrosis)를 유도한다는 것을 관찰하였다. nude mice (Balb-C)에 SK-OY-3 난소암 세포를 주사한 in vivo 실험에서 hSP는 주요 장기에 영향없이 종양 세포괴사를 유도했다. 결론 : 본 연구는 hSP가 상피성 난소암 세포에 세포독성 효과가 있음을 증명하는 실험이었으며, 따라서 hSP는상피성 난소암에 대한 안전한 치료적 방법으로 사용될 수 있는 과학적 기초를 제공할 것이다. Objective : From knowledge of risk factors of epithelial ovarian cancer, we deduced a hypothesis that a reduced exposure to human seminal plasma (hSP) during the period of ovulation is one of etiological risk factors in the development of epithelial ovarian cancer and hSP contribute to eliminate maltransformed epithelial cells. Materials & Methods : To examine whether hSP directly influence the growth of ovarian cancer, we have been investigated the in vivo and in vitro effects of hSP on epithelial ovarian cancer cells (SK-OV-3, OVCAR-3) and, as a control, we also tested its effect in cervical cancer cell (CaSki) and fibroblast cell (WI-38). In in vitro MTT assays of varian cancer cells, we found hSP induces necrosis at a final concentration of 1:50 dilution, whereas cervical cancer cell and fibroblast cell were not affected. The flowcytometric assay, DNA laddering, biochemical assay and morphological analysis indicated that hSP induced necrosis rather than apoptosis of both ovarian adenocarcinoma cell lines. The morphological alterations of hSP-treated SK-OV-3 cells were confirmed by transmission electron microscopy that demonstrated swollen mitochondrias with partially or completely destroyed cristae and shrinkage of nucleus with margination and condensation of chromatin, and intranuclear vesiculation. These findings strongly suggest that hSP induced necrosis of ovarian cancer cell lines through destruction of mitochondria. In in vivo experiments that used the nude mice (Balb-C) with tumor by inoculation of SK-OV-3 ovarian cancer cell line, hSP induced necrosis of tumor with no detectable toxic effects on the major organs. Conclusion : Our study supported the hypothesis that hSP has a potent cytotoxic effect on epithelial ovarian cancer cells. Therefore, hSP can be used as a safe, promising therapeutic agent for the epithelial ovarian cancer and may provide a scientific basis for preventing epithelial ovarian cancer by direct exposure to seminal plasma without barrier and withdrawal method for contraception.
이헌영,김수평,이종건,박래옥,정민화,차정일 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.1
본 교실에서는 임신 및 초기 산욕기에 발생 또는 합병된 HELLP증후군 환자 2예, TTP 환자 1예, ITP환자 1예를 경험하였고, 이들에게 혈장교환을 시행하여 매우 성공적인 치료효과를 얻었으므로 문헌고찰과 함께 보고하는 바이다. Plasmapheresis, a process in which blood is withdrawn, the plasma removed, and the red cells reinfused after resuspension in a replacement solution, has been used for a variety of conditions during complicated pregnancy and puerperium. Two cases of HELLP syndrome, 1 case of thrombotic thrombocytopenic purpura and 1 case of immune thrombocytopenic purpura complicating pregnancy who were treated with plasmapheresis were presented. We also discussed the possible role of plasmapheresis with fresh frozen plasma in treating these selected cases for maternal indications.