목이버섯의 항비만 효과

박기문,김길자,장갑열,박건희 한국버섯학회 2018 한국버섯학회지 Vol.16 No.2

The purpose of this study is to discover a food material having anti-obesity effects and to disseminate information on the effects of the material to people who are interested in anti-obesity. For this study, 11 kinds of Auricularia (wood ear) spp., including 8 strains of Auricularia auricula-polytricha, and 3 strains of A. auricula-judae, were presented by the Jeollanamdo Agricultural Research & Extension Services. 3T3-L1 (preadipocyte cell) was used for identifying the inhibition effect on adipocyte differentiation. As a result, this study found that all the extracts had slightly different degrees of inhibition effects on adipocyte differentiation. Among the A. auricula-polytricha strains, strain 21001 showed the most significant effect (4.58%), and the inhibition effect of strain 21002 (4.43%) was the greatest among A. auricula-judae strains. Overall, the inhibition effect of A. auricula-polytricha strains was greater than that of A. auricula-judae strains. The results of mRNA and protein analysis also demonstrated that the inhibition effect of A. auricula-polytricha 21001 was superior to that of any other strains. An in vivo study using 56 ICR mice (6w, male), was performed for 4 weeks. A. auricula-polytricha 21001, which exhibited the most significant effect in the in vitro study was used to compose six different types of feeds. Daily body weight gain of the high-fat diet containing 0.2% 21001 extract feeding group was 0.22±0.08 g (*p < 0.05), and it was 31.25% lower than that of the high-fat diet feeding group (0.32±0.06). Internal organ weight measurement and blood analysis were performed immediately after animal sacrifice. The results proved that treatment with more than 0.1% of A. auricula-polytricha strain 21001 could significantly reduce (more than *p < 0.05) the weight of liver and epididymal fat, and levels of glucose, total cholesterol, AST, and ALT in blood.

Determination of Kinetic Parameters of Growth and Carotenogenesis in the Red Yeast Xanthophyllomyces dendrorhous

박기문,Min Woo Song,이재흥 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.4

Glucose-limited continuous culture experiments with Xanthophyllomyces dendrorhous ATCC 24202 were carried out in a 2.5 L jar fermentor at 20o C and pH 4.5. A reciprocal plot of the steady-state data at a dilution rate of 0.037~0.096 h-1 was used to estimate a maximum specific growth rate constant of 0.11 h-1 and a Monod constant of 260 mg/L. The degree of carotenogenesis in X. dendrorhous was also investigated in terms of the residence time, which is the reciprocal of the dilution rate. The carotenoid content related to the residence time appeared to assume a typical form of saturation kinetics. The maximum carotenoid content for the strain was estimated to be 0.6 μg/mg dry cell weight. The saturation constant, which was tentatively defined in this work, was found to be 7.2 h.

Production of Carotenoids by β-Ionone-Resistant Mutant of Xanthophyllomycesdendrorhous Using Various Carbon Sources

박기문,Min Woo Song,이재흥 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.2

Batch culture kinetics of the red yeast, Xanthophyllomyces dendrorhous SKKU 0107, revealed reduction in biomass with glucose and lower intracellular carotenoid content with fructose. Figures were different when compared to sucrose, which is a disaccharide of glucose and fructose. In contrast, specific growth rate constant stayed between 0.094~0.098 h-¹, irrespective of the carbon sources employed. Although the uptake rate of glucose was found to be 2.9-fold faster than that of fructose, sucrose was found to be a more suitable carbon source for the production of carotenoids by the studied strain. When sugar cane molasses was used, both the specific growth rate constant and the intracellular carotenoid content decreased by 27 and 17%, respectively. Compared with the batch culture using 28 g/L sugar cane molasses, fed-batch culture with the same strain resulted in a 1.45-fold higher cell yield together with a similar level of carotenoid content in X. dendrorhous SKKU 0107.

[P5-26] 쌀뜨물을 기질로 이용한 Biobutanol 생산에 관한 연구

박기문,이지은,서은종,이상훈,유진희 한국식품영양과학회 2008 한국식품영양과학회 학술대회발표집 Vol.2008 No.10

SWOT분석을 통한 발명영재교육의 발전방향 탐색 -국가지정 영재교육연구원 중심으로-

박기문,이규녀 한국기술교육학회 2012 한국기술교육학회지 Vol.12 No.3

This study is aimed at identifying research trends of national gifted education research institute and proposing development direction and strategies based on the strategies deduced through SWOT analysis. The conclusions of this study are as follows. First, the analysis results on the research and business data of national gifted education research institute showed that although there exist some differences in weight of business depending on the purpose of its establishment, the business commonly shared by the gifted education research institute may include basic research on the gifted education and its policy, development of gifted education methods and materials, and development of teacher training materials and education. Second, it turned out that invention gifted education support agencies are focused on the research and development of invention gifted education methods, materials and a screening for gifted students. The tendency to be concentrated on 1 to 2 sectors poses its limitation of quantitative expansion and quality enhancement of gifted education research and education, and as a measure to overcome this problem, it is urgently required to establish national invention gifted education research institute equipped with invention gifted education and research functions. Third, from the results of SWOT analysis for inquiry into the development direction of invention gifted education, 4 strength factors, 3 weakness factors, 5 opportunity factors and 4 thereat factors were deduced. Fourth, the invention gifted education development measures through SWOT analysis formulated 4 SO strategies to highlight strengths utilizing opportunities, 3 WO strategies to maximize opportunities by overcoming weaknesses, 4 ST strategies to utilize strengths for minimizing threats, and 3 WT strategies to avoid threats and complement weaknesses. In conclusion, to strengthen research capabilities of invention gifted education and improve its quality, it is required to establish national gifted education institute on the invention gifted education and prepare operational strategies differentiated from other gifted education research institutes through strengthening personalized growth support system like PCU. In addition, it is of utmost necessity to formulate mid-to-long term development plans for invention gifted education and the relevant legal basis. 이 연구는 국가지정 영재교육연구원의 연구 동향을 살펴보고 SWOT분석기법을 통하여 도출된 전략을 바탕으로 발명영재교육의 발전방향과 전략을 제시하는데 그 목적이 있다. 이 연구의 결론은 다음과 같다. 첫째, 국가지정 영재교육연구원의 연구 및 사업 자료를 분석한 결과, 각 영재교육연구원은 설립 목적에 따라 업무의 비중이 다소 차이가 있으나, 공통적인 업무는 영재교육에 대한 기초 및 정책 연구, 영재교육방법 및 자료의 연구 개발, 교원연수 자료 연구 개발 및 연수이다. 둘째, 타 영재교육 지원기관과 달리 발명영재교육 지원기관은 발명영재교육방법 및 자료의 연구 개발과 영재판별에 관한 연구 개발에 편중되어 있는 것으로 나타났다. 1~2개 분야에 편중된 경향은 발명영재교육 연구와 교육의 양적 확대와 질 제고의 한계점으로 작용하고 있으며, 이를 극복하기 위해서는 발명영재교육 및 연구 기능을 갖춘 국가지정 발명영재교육연구원의 설립이 시급하다. 셋째, 발명영재교육 발전방안을 모색하기 위해 SWOT 기법으로 분석한 결과, 강점요인은 4개, 약점 요인은 3개, 기회요인은 5개, 위협요인은 4개가 도출되었다. 넷째, SWOT 분석기법을 통한 발명영재교육 발전방안은 기회를 활용하여 강점을 부각하는 강점(S)-기회(O)전략 4개, 약점을 극복함으로 기회를 최대화하는 약점(W)-기회(O)전략 3개, 위협 최소화를 위하여 강점을 활용하는 강점(S)-위협(T)전략 4개, 위협을 회피하고 약점을 보완하는 약점(W)-위협(T)전략 3개을 구안하였다. 결론적으로 발명영재교육의 연구 역량 강화와 질적 개선을 위해서는 발명영재교육에 관한 국가지정 영재교육교육기관이 필요하며, PCU와 같은 개인 맞춤형 성장지원체제를 강화하여 타 영재교육연구원과의 차별화 운영 전략도 마련이 필요하다. 더불어 발명영재교육의 중장기 발전계획뿐만 아니라 이에 따른 법적 근거 마련이 절실하다.

학교교육에서 융합인재교육의 BARS 기반 핵심역량 평가도구 개발

박기문,이규녀 한국기술교육학회 2016 한국기술교육학회지 Vol.16 No.1

This study is aimed at developing and validating BARS (Behaviorally Anchored Rating Scale)-based key competencies assessment tool for STEAM in school education, and giving fundamental data regarding the STEAM competency assessment. The results of this study are as follows. First, BARS-based assessment tool for observing and evaluating learner’s behavior characteristic, by applying learning outcome from STEAM of school education, as key competencies, was conceived by literature review, and its validity was secured by carrying out a survey targeting professionals. Second, the grade of developed assessment tool was divided into four stages, and the behavior identification standards for assessment of key competencies, consisted of seven capabilities of convergence cognitive, eleven capabilities of convergence cognitive and four capabilities of convergence attitude, before developing achievement standards for each level. Third, with regard to the application of developed assessment tool, objectivity and fairness of the assessment results of key competencies should be assured, and the contextual environment should be considered by applying in the elementary and middle schools. For this, it is necessary to reflect the relative weight for each key competency. 이 연구는 학교교육에서 융합인재교육의 BARS(Behaviorally Anchored Rating Scale) 기반 핵심역량 평가도구를 개발 및 타당화하여 융합인재교육의 역량 평가에 관한 기초자료를 제공하는 데 목적이 있으며, 이에 따른 연구의 결론은 다음과같다. 첫째, 학교교육의 융합인재교육에 따른 학습성과를 핵심역량으로 적용하여 학습자의 행동특성을 관찰 및 평가하기 위한 BARS 기반 평가도구는 문헌고찰 방법으로구안하고 전문가 대상 설문조사를 통하여 타당성을 확보하였다. 둘째, 개발한 평가도구의 수준은 4개 수준으로 설정하고, 핵심역량을 평가하기위한 행동식별기준에는 융합인지능력 7개, 융합수행능력 11개, 융합태도능력 4개를구성 및 분류하고 수준별 성취기준을 개발하였다. 셋째, 개발한 평가도구가 핵심역량 평가결과의 객관성과 공정성을 담보하고 초・ 중등학교에서의 현업활용도를 확보하는 방안으로 맥락적 환경을 고려할 필요가 있다. 이를 위해서는 핵심역량별 상대적 가중치를 반영할 필요가 있다.

연차학술대회 발표논문 : 토론 ; 교과서 구성과 교육과정의 연계성

박기문 한국교육과정학회 1998 교육과정연구 Vol.16 No.1

융합인재교육의 핵심역량 구성요인에 대한 타당성 연구

박기문,최유현,홍준희,이규녀,문성환,태진미,이경표,민봉기,노경숙 한국기술교육학회 2014 한국기술교육학회지 Vol.14 No.3

이 연구는 융합인재교육의 프로그램을 투입한 다음에 기대되는 학습성과의역량평가 준거를 제시하기 위하여 핵심역량에 대한 타당성을 조사․분석하고자한다. 연구방법은 과학, 기술/공학, 인문/예술, 수학 분야 등의 전문가 10명을대상으로 타당성 설문조사와 전문가 협의회를 실시하였다. 연구결과에 따른 결론 및 제언은 다음과 같다. 첫째, 창의적 융합인재를 양성하기 위한 융합인재교육에서 기대되는 학습성과 핵심역량은 융합인지능력과 융합수행능력, 융합태도능력 3개 준거로 분석되었다. 둘째, 개발된 핵심역량의 융합인지능력은 3개 준거(창의적 사고, 비판적 사고, 융합지식 이해), 융합수행능력은 4개 준거(문제해결, 의사소통, 협동, 융합도구 활용), 융합태도능력은 2개 준거(배려심, 책임감)로 분석되었다. 셋째, 융합인재교육의 핵심역량에 초점을 두고 향후에는 핵심역량 평가방법을 연구·개발하고 현장 적용 연구가 필요하다. This study aims to investigate/analyze validity of key competencies in order toprovide evaluation criteria for study outcome which is expected after running theSTEAM program. As a research method, validity surveys and expert consultationwere conducted for the 10 experts in the fields of science, technology/engineering,humanities/arts, and mathematics. The conclusions and suggestions from this studyare as follows. First, the key competencies of study outcome from STEAM were identified ascapabilities of convergence cognitive, capabilities of convergence accomplishment,and capabilities of convergence attitude. Second, capabilities of convergence cognitive were analyzed by 3 criteria(creative thinking, critical thinking, understanding fusion knowledge); capabilities ofconvergence accomplishment by 4 criteria (problem solving, communication,collaboration, utilizing convergence tools); capabilities of convergence attitude by 2criteria (thoughtfulness, responsibility). Third, research/development of evaluation methods for key competencies andtheir application are necessary in the future while focusing on key competencies of STEAM.

Xanthophyllomyces dendrorhous 변이군주에 의한 Carotenoids 생산 발효의 특성 연구

박기문,김영준,송민우,강석진,이재흥,Park, Ki-Moon,Kim, Young-Jun,Song, Min-Woo,Kang, Seog-Jin,Lee, Jae-Heung 한국생물공학회 2006 KSBB Journal Vol.21 No.4

Various ${\beta}$-ionone resistant mutants were isolated from the wild-type red yeast Xanthophyllomyces dendrorhous KCTC 7704. Although the growth of X. dendrorhous KCTC 7704 was strongly inhibited at 0.025 mM ${\beta}$-ionone, one of the ${\beta}$-ionone resistant mutants isolated at 0.1 mM ${\beta}$-ionone by NTG mutagenesis showed rather 70% of relative survival at 0.15 mM ${\beta}$-ionone. Fermentation kinetics study with the mutant was carried out at $20^{\circ}C$ for 4 days in 300-mL baffled flasks. The mutant yielded up to 2.3-fold higher carotenoids content(viz. $1.2{\mu}g$ of total carotenoids per mg of dry cells) compared with the wild-type strain. The production of metabolites such as organic acids could be neglected. Studies on the kinetics with various carbon substrates revealed both an increase in final dry cell mass and a higher total carotenoids content in cell mass with glucose when compared to fructose or sucrose. As a further part of study, the effect of pH on the fermentation kinetics was investigated in glucose-limited chemostat at a dilution rate of $0.04h^{-1}$. When compared to steady-state kinetic parameters obtained at pH 4.0, a significant reduction in cell concentration at pH 3.0 and a lower carotenoids content at pH 5.2 were evident. 본 연구에서는 적효모 X. dendrorhous KCTC 7704로부터 여러 ${\beta}$-ionone 내성 변이균주를 선별하였다. 야생균 KCTC 7704는 ${\beta}$-ionone 0.021 mM 농도에서 생육이 현저히 저하되었지만, NTG처리 후 ${\beta}$-ionone 0.1 mM 농도에서 선별된 변이균주는 ${\beta}$-ionone 0.15 mM에서도 70% 이상의 상대 생육율을 나타내는 매우 강한 ${\beta}$-ionone 내성을 갖고 있었다. 여러 ${\beta}$-ionone 농도에서 선별한 변이균주들을 $20^{\circ}C$에서 4일간 회분식 발효로 배양하여 그 특성을 조사하였다. 선별된 가장 우수한 변이균주는 야생균주보다 카로티노이드 생성능이 2.3배 향상(즉 $1.2{\mu}g$ of total carotenoids per mg of dry cells)되었으며 유기산과 같은 대사산물은 거의 생성하지 않았다. 여러 탄소원 들에 대한 비교 발효특성 연구 결과 과당이나 자당을 사용했을 때봐 비교하여 포도당 배지에서 최종 균체농도 및 총 카로티노이드 생성량이 많았다. 포도당이 제한되는 연속발효(dilution rate $0.04h^{-1}$) 실험을 통하여 pH의 영향을 조사한 결과 균체농도 및 총 카로티노이드 생성은 pH 4.0 조건하에서 최적인 것을 알 수 있었다.

[P8-124] 표고버섯 추출물이 항암활성에 미치는 영향

박기문,양희진,유진희,이다형 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11