대황의 항산화 효과 및 타이로시네이즈 저해 활성

김정은 ( Jung Eun Kim ),박찬하 ( Chan Ha Park ),오대석 ( Dae Seok Oh ),이승연 ( Seung Yeon Lee ),장세훈 ( Se Hun Jang ),홍지연 ( Jee Yeon Hong ),민혜진 ( Hye Jin Min ),박수아 ( Su Ah Park ),원두현 ( Doo Hyun Won ),박수남 ( Soo Nam 대한화장품학회 2011 대한화장품학회지 Vol.37 No.4

본 연구에서는 대황 추출물의 항산화 활성, 타이로시네이즈(tyrosinase) 저해 활성을 확인하였다. 대황의 50 % 에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획으로 실험을 진행하였다. 대황 추출물들의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성(FSC₅₀)은 대표적인 항산화제인 (+)-α-tocopherol보다 낮은 것으로 나타났다. Luminol 발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종에 대한 아글리콘 분획의 소거활성(총 항산화능, OSC₅₀)은 0.265 μg/mL로 매우 큰 활성을 나타내었다. 대황 추출물의 rose-bengal로 증감된 ¹O₂에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적(1∼50 μg/mL)으로 증가하였으며, 특히 아글리콘 분획은 10 μg/mL 농도에서 τ₅₀이 757.0 min으로 높은 세포 보호 활성을 나타내었다. 대황 추출물 중 아글리콘 분획의 타이로시네이즈 저해활성(IC₅₀)은 11.20 μg/mL으로 226.88 μg/mL인 알부틴(arbutin)보다 큰 활성을 보여주었다. 이상의 결과들로부터 대황 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 아글리콘 분획의 현저한 항산화 작용 및 큰 타이로시네이즈 저해 효과로부터 이들 분획 또한 화장품원료로서 응용 가능성이 큼을 알 수 있었다. In this study, the antioxidative effects and inhibitory activities on tyrosinase of Rheum undulatum (R. undulatum) L. extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of R. undulatum L. were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC₅₀) of R. undulatum L. extracts was lower than (+)-α-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of aglycone fraction on ROS generated in Fe³⁺-EDTA/H₂O₂ system using the luminol-dependent chemiluminescence assay showed the most prominent effect at a concentration of 0.265 μg/mL. The cellular protective effects of extract/fractions of R. undulatum L. on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1 ~ 50 μg/mL). Especially, aglycone fraction in 10 μg/mL concentration showed the most protective effect among extracts (τ₅₀, 757.0 min). The inhibitory effects (IC₅₀, 11.20 μg/mL) on tyrosinase of aglycone fraction was much higher than arbutin (226.88 μg/mL), known as a whitening agent. These results indicate that R. undulatum L. extracts can be used as antioxidant. Particularly, aglycone fraction of R. undulatum L. showed superior antioxdative activity and high inhibitory effect on tyrosinase. Therefore, aglycone fraction of R. undulatum L. could be applicable to new functional cosmetics.

정신장애인 가족의 낙인감이 자아존중감에 미치는 영향

민혜진(Hye Jin Min),정원철(Weon Cheol Jeong) 한국장애인재활협회 2016 재활복지 Vol.20 No.1

본 연구는 정신장애인 가족의 낙인지각이 가족의 삶의 질을 방해한다는 전제에서 정신장애인 가족의 낙인감이 자아존중감에 미치는 영향에서 사회적 지지가 조절효과로 기능하는 지를 경험적으로 검증하고자 하였다. 이를 위해 자료수집은 부산, 경남 지역의 사회복귀시설 10곳, 낮병원 4곳 총 190명의 가족을 대상으로 자료를 수집하였다. 연구결과 정신장애인 가족의 낙인감은 자아존중감과 사회적 지지에 부적 영향을 미치며, 사회적 지지는 자아존중감에 정적인 영향을 미치는 것으로 확인되었다. 또한 정신장애인 가족의 낙인감은 자아존중감에 직접적인 영향을 미치며, 사회적 지지라는 조절변수를 통해 자아존중감에 간접적으로 영향을 미치는 것으로 파악되었다. 즉 가족의 낙인감이 높을수록 자아존중감이 낮아지는 관계에서 사회적 지지가 조절변인으로 투입되었을 때 자아존중감이 상승한다는 것을 의미한다. 본 연구는 정신장애인 가족의 낙인지각이 자아존중감을 손상시키며, 사회적 지지가 주어질 경우 낙인을 지각함에도 불구하고 이들의 자아존중감이 유지되거나 상승될 수 있음을 경험적으로 입증했다는 점에 의의가 있다. 본 연구결과에 따라 정신장애인 가족들을 대상으로 사회적 지지를 제공할 수 있는 방안과 연구결과의 함의를 논의하였다. The purpose of this study is to verify the moderating effects of social supports in the relationship between stigma and self-esteem of the mental disabled s family. For this research, 190 family of the mental disabled were asked to complete some scales for this study. The results of this study are as follows. First, stigma of the mental disabled s family is positively correlated to self-esteem and social supports. Second, social support is positively correlated to self-esteem. Third, social supports have moderating effect the relationship between stigma and self-esteem of the mental disabled s family. This study verify that for the purpose of decreasing the stigma and improving the self-esteem, it is important to supply the mental disabled s family with social supports. This study have implication that stigma of the mental disabled family impair their self esteem and social supports delivered to the mental disabled s family improve family s self esteem. In accordance to the results of this study, the authors suggest some strategy to supply social support to the family. The authors suggest some direction to solve the problems related this study questions and describe the limitation of this study.

C<SUB>5</SUB>계 수소불화에테르를 기반으로 하는 배합 세정제의 CFC 대체세정제 적용 연구

민혜진(Hye Jin Min),배재흠(Jae Heum Bae),장윤상(Yoonsang Chang) 한국청정기술학회 2010 청정기술 Vol.16 No.3

CFC 세정제의 제3 세대 대체세정제로 평가되는 수소불화에테르(hydrofluoroether; HFE)는 불소분자를 함유하고 있어 표면장력이 낮고 습윤지수가 커서 미세입자제거와 불소계 오염물 제거에 우수하고 인화점이 없고 건조성이 뛰어난 물성을 가지고 있지만, 유기물의 용해력은 떨어져 유기 오염물 제거에는 효율적이지 못하다. 본 연구에서는 C5계 HFE인 HFE-7100 [CF₃CF₂CF₂CF₂OCH₃]와 HFE-mec-f[CF₃CHF=CF₂OCH₂CF₃]에 유기물질의 용해력이 비교적으로 뛰어난 isopropyl alcohol (IPA), ethyleneglycol monoether (EG), propyleneglycol monoethylether (PM)를 인화점이 나타나지 않는 최대량을 각각 첨가하여 C5계 HFE 기반의 배합세정제를 제조하여 물성과 여러 종류의 플럭스, 비수용성 절삭유, 불소계 오일에 대한 세정성 평가를 수행하였다. 실험 결과 C5계 기반의 배합세정제는 단일 불소계 세정제의 여러 가지 물리적 성질의 장점을 가지는 것으로 나타났다. 그리고 C5계 기반의 배합세정제는 첨가된 용제에 따라 약간의 차이는 있지만 대체적으로 플럭스, 비수용성 절삭유, 불소계 오일에 우수한 세정력을 나타내어 여러 산업세정분야에 적용가능성을 보여주었다. Hydrofluoroethers (HFEs) with fluoride molecules in their structure which are evaluated as the third generation replacement alternatives to chlorofluorocarbons (CFCs) are known to be excellent for removal of nanoparticles and fluoride-type soils due to their low surface tension and high wetting index. In addition, HFEs have good physical properties with no flash point and excellent drying characteristics. But, HFEs also have shortcomings in that they are not effective for removal of organic soils due to their poor solubility in soil. In this study, C5 HFE-based cleaning agents were formulated through addition of solvents such as isopropyl alcohol (IPA), ethyleneglycol monoether (EG), propyleneglycol monoethylether (PM) to HFE-7100 [CF₃CF₂CF₂CF₂OCH₃] or HFE-mec-f [CF₃CHF=CF₂OCH₂CF₃] with its maximum amount, respectively, in order to have no flash point for the safety in the working environment. These solvents are known to be excellent for dissolving organics in soil. Their physical properties and cleaning abilities for fluxes, water-insoluble cutting oils, and fluoride-type oils were evaluated and compared with those of other cleaning agents with single components. The experimental results show that the HFE-based formulated cleaning agents have various good physical properties which are almost similar to those of a single type of HFE cleaner. They show excellent cleaning ability for fluxes, water-insoluble cutting oils, and fluoride-type oils. These results indicate that the HFE-based formulated cleaning agents can be applicable to various industrial cleaning fields because of their good physical properties and cleaning abilities for various soils.

CFC 대체 산업세정제로의 HFEs의 적용가능성 연구

민혜진(Hye Jin Min),신진호(Jin Ho Shin),배재흠(Jae Heum Bae),김홍곤(Hong Gon Kim),이현주(Hyun Joo Lee) 한국청정기술학회 2008 청정기술 Vol.14 No.3

불소계 화합물인 삼불화에탄올(2,2,2-trifluoroethanol, TFEA)와 수소화불화에테르(hydrofluoroether, HFE)는 성층권의 오존층을 파괴하지 않으며, 수소화불화탄소(hydrofluorocarbon, HFC) 및 수소화염화불화탄소(hydrochlorofluorocarbon, HCFC)계 세정제에 비하여 지구온난화 영향력도 낮다. 특히, 인화점이 없는 HFE계 세정제는 낮은 인화점을 갖는 탄화수소계 세정제보다 취급에 안전하고 침투력이 우수하여 차세대 염화불화탄소(chlorofluorocarbon, CFC) 대체세정제로 주목받고 있다. 본 연구에서는 불소계 세정제인 TFEA, HFE-7100, HFE-7200, HFE-476mec, HFE-449mec-f, AE-3000, AE-3100E와 실리콘계 세정제인 트리플루로에톡시트리메틸실란(trifluoroetoxytrimethylsilane, TFES)와 헥사메틸디실라잔 (hexamethyldisilazane, HMDS)의 물성과 세정력을 오존파괴 물질인 CFC-113, 삼염화에탄(1,1,1-trichloroethane)와 유해물질인 이염화탄소(methylene chloride, MC) 등의 염소계 세정제, 그리고 현재 대체세정제로 많이 사용하고 있는 이소프로필알코올(isopropyl alcohol, IPA) 등과 비교 평가하였다. 그 결과 TFEA와 HFEs는 일반적으로 발생하는 다양한 오염물질들에 대하여 염소계 세정제에 비하여 낮은 세정능력을 보여주었지만, 불소를 함유한 불소계 오염물의 제거에는 우수한 세정력을 보였다. 그리고 실리콘계 세정제인 TFES와 HMDS도 실리콘계 오염물에 대한 세정력이 우수함을 보여주었다. Fluoride-type cleaning agents such as 2,2,2-trifluoroethanol (TFEA) and hydrofluoroethers (HFEs) do not destroy ozone in the stratosphere and have low global warming potential compared to hydrofluorocarbons(HFCs) and hydrochlorofluorocarbons (HCFCs). Especially, HFEs which have no flash point are paid attention as next generation type of cleaning agents for chlorofluorocarbons (CFCs) since they are safe in handling and have excellent penetration ability compared to hydrocarbon cleaning agents with low flash point. Here, the physical properties and cleaning abilities of fluoride-type cleaning agents such as TFEA, HFE-7100, HFE-7200, HFE-476mec, HFE-449mec-f, AE-3000 and AE-3100E and silicide-type cleaning agents such as trifluoroetoxytrimethylsilane (TFES) and hexamethyldisilazane (HMDS) were measured and compared with those of ozone destruction substances such as CFC-113 and 1,1,1-trichloroethane. They were also compared with toxic methylene chloride (MC) and isopropyl alcohol (IPA) which are now being used as an alternative cleaning agents. As a result, TFEA and HFEs had lower cleaning ability for removal of various soils compared to chloride-type cleaning agents, but they showed excellent cleaning ability for fluoride-type soils. TFES and HMDS also showed excellent cleaning ability for silicide-type soils.

표현 대상과 노출 대상을 고려한 대화문장의 감정 파악

민혜진(Hye-Jin Min),박종철(Jong C. Park) 한국HCI학회 2007 한국HCI학회 학술대회 Vol.2007 No.2

발레 전공 교수자의 유형별 효율적 교수행동에 대한 중요도와 실천도 차이

민혜진(Min, Hye-Jin) 한국체육과학회 2021 한국체육과학회지 Vol.30 No.2

The purpose of this study is to analyze a difference between importance and practice on efficient teaching behaviors according to a type-based characteristic of instructors with a major in ballet. A research method was carried out independent sample t-test in order to empirically verify whether the importance and the perception on instructors’ efficient teaching-behavior factors in dependent variable have a difference depending on a type-based characteristic in instructors. Summarizing the specific findings, they are as follows. First, it is the outcome that parsed a difference in importance and perception on instructors’ efficient teaching behaviors according to a trainer’s age. The recognition on importance was created a difference between the ages of instructors in interaction, fellowship and relevance. The practice was occurred a difference between the ages of instructors in fellowship, relevance and flexibility. Second, it is the result that dissected a difference in importance and perception on instructors’ efficient teaching behaviors depending on a trainer’s educational institution. The awareness on importance was happened a difference between instructor teachers’ educational institutions in clarity, interaction, fairness, fellowship and learning-centered. The practice was created a difference in clarify between instructors’ general characteristics. Third, it is the outcome that analyzed a difference in importance and perception on instructors’ efficient teaching behaviors according to an instructor’s education career. The recognition on importance was created a difference in a narrative skill between instructors(teachers)’ general characteristics(education career). The practice was occurred a difference between instructors’ education careers in all factors such as passion, clarify, interaction, systematization, speed, fairness, narrative skill, fellowship, relevance, learning-centered, flexibility and leadership. Fourth, it is the result that parsed a difference between importance and practice on instructors’ efficient teaching behaviors. A difference between instructors(teachers)’ general characteristics(group with importance/practice) in all factors such as passion, clarify, interaction, systematization, speed, fairness, narrative skill, fellowship, relevance, learning-centered, flexibility and leadership.

언어학적 단서를 활용한 동화 텍스트 내 발화문의 화자 파악

민혜진 ( Hye Jin Min ),정진우 ( Jin Woo Chung ),박종철 ( Jong C. Park ) 한국언어정보학회 2013 언어와 정보 Vol.17 No.2

Identifying the speakers of individual utterances men-tioned in textual stories is an important step towards developing applications that involve the use of unique characteristics of speakers in stories, such as robot storytelling and story-to-scene generation. Despite the usefulness, it is a challenging task because not only human entities but also animals and even inanimate objects can become speakers especially in fairytales so that the num-ber of candidates is much more than that in other types of text. In addition, since the action of speaking is not always mentioned explicitly, it is necessary to infer the speaker from the implicitly mentioned speaking behaviors such as appearances or emotional expressions. In shis paper, we investigate a method to exploit linguistic cluses to identify the speakers of utterances from textual fairytale stories in Korean, especially in order to handle such challenging issues. Compared with the previous work, the present work takes into accout addi-tional linguistic features such as vocative roles and pairs of conversation partic-ipants, and proposes the use of discourse-level turn-taking behaviors between speakers to further reduce the number of possible candidate speakers. We de-scribe a simple rule-based method to choose a speaker form candidates based on such linguistic features and turn-taking behaviors. (Korea Advanced In-stitute of Science and Technology)

u-SPACE: 육아 보조 및 맞춤 교육을 위한 유비쿼터스 시스템

민혜진(Hye-Jin Min),박두진(Doojin Park),장은영(Chang Eunyoung),이호준(Ho-Joon Lee),박종철(Jong C. Park) 한국HCI학회 2006 한국HCI학회 학술대회 Vol.2006 No.2

결합범주문법을 이용한 멀티미디어 치료 정보의 자동 생성

민혜진(Hye-Jin Min),박종철(Jong C. Park) 한국HCI학회 2004 한국HCI학회 학술대회 Vol.2004 No.2

광전자방출(OSEE)법을 이용한 세정성 평가 연구

민혜진(Hye Jin Min),신진호(Jin Ho Shin),배재흠(Jae Heum Bae) 한국청정기술학회 2008 청정기술 Vol.14 No.2

친환경적인 대체 세정제를 채택하기 위해서는 세정제의 세정성, 환경성, 경제성을 평가하여 체계적인 선정절차에 의거하여 도입ㆍ적용하여야 하며 객관적이고 효율적인 세정성 평가방법의 정립이 현시점에서 매우 중요하다. 따라서 본 연구에서는 여러 세정성 평가 방법들 중 광전자방출법(optically stimulated electron emission)(OSEE)을 활용한 세정성 평가에 관한 연구를 하였다. 실험에 사용된 오염물은 플럭스, 솔더, 그리스, 절삭유, 그리고 그리스 35% 와 절삭유 65%을 혼합하여 만든 혼합 오염물로서 세정제에 의한 이들의 세정성을 평가하였다. 본 연구실에서 개발하였거나 조제한 세정제를 이용하여 세정 후 OSEE법 측정 결과와 중량법 및 접촉각 측정법에 의한 세정성 평가방법을 비교 분석하였다. 본 연구 결과로 플럭스와 혼합오염물을 세정한 경우의 OSEE 세정효율은 중량법과 거의 유사한 결과를 나타내었지만, 솔더와 그리스 세정의 경우는 UV를 반사 또는 흡수하여 중량법과 비교할 수 없었다. 그리고 절삭유 세정의 경우 실질적으로 눈에 보일 정도로 피세정물표면에 오염물이 남아 있음에도 불구하고 중량법 측정 시 100%의 세정효율을 보임으로써 중량법 측정의 한계를 보였지만 OSEE법으로는 미소잔류 절삭유의 존재를 측정할 수 있었다. 또한 OSEE법과 접촉각측정법의 세정성을 비교측정한 결과로 플럭스, 혼합 오염물 그리고 절삭유의 세정성 평가는 OSEE법과 접촉각측정법이 유사한 세정효율을 보여주었다. 그리고 솔더와 그리스 세정은 접촉각측정법이 OSEE법보다 더욱 정밀하게 세정성을 평가할 수 있는 것으로 판단되었다. In order to choose alternative environmental-friendly cleaning agents, it is very important in the present point that the systematic selection procedures should be introduced and applied to the industry through the evaluation of their cleaning ability, environmental characteristics, and economical factors, and that the objective and effective evaluation methods of cleanliness should be established for the industry. Thus, a novel cleaning evaluation method utilizing optically stimulated electron emission (OSEE) among various methods of cleaning ability was studied in this study. The contaminants used in this cleaning experiments were flux, solder, grease, cutting oil, and mixed soil of 35% grease and 65% cutting oil. The cleaning agents developed or prepared in our laboratory were employed and their cleaning ability were comparatively evaluated by the OSEE, gravimetry and contact angle methods. The experimental results in this work showed that flux cleaning efficiency measured by the OSEE method was similar to that of the gravimetric method, but that the OSEE method could not be compared with gravimetric method for the case of solder or grease cleaning because the contaminants radiate or absorb ultra-violet light. In case of cutting oil cleaning, the gravimetric method indicated its limitation of cleaning efficiency of cutting oil since it showed cleaning efficiency of 100%, even though residual soil remaining on the substrate surface a little after its cleaning. The comparative experimental results of cleaning ability evaluated by the OSEE- and contact angle measurement methods showed that they were similar in case of cleaning of flux, mixed soil and cutting oil. It was judged that the contact angle measurement method could evaluate the cleaning ability more precisely than the OSEE method for cleaning solder and grease.