광전자증폭관에 의한 키토산 첨가 사료를 사용한 양돈 생체조직에서의 육질 품질 비교 측정

민제호,유종수,천병수,Min, Je-Ho,Yoo, Jong-Su,Cheun, Byeung-Soo 한국전자통신학회 2014 한국전자통신학회 논문지 Vol.9 No.3

키토산 첨가 혼합사료를 섭취한 양돈 근육 조직과 일반 사료를 섭취한 조직에서의 품질을 비교하기 위한 방법으로 생체광자를 이용한 광전자증폭관(Photomultiplier Tube, PMT) 기기를 사용하여 측정한 결과 서로 다른 결과를 얻었다. 키토산 첨가제를 사용한 돼지 등심조직에서의 육질 비교를 PMT와 현미경을 사용하여 측정한 결과 키토산 첨가 혼합 사료를 먹인 돼지 등심 조직의 생체광자 양을 측정한 결과 서로 상이한 수치를 얻을 수 있었다. 이것으로 조직의 품질을 비교 측정이 가능했다. 또한 이조직의 형태를 해부현미경으로 관찰한 결과 키토산 첨가 혼합사료 근육 조직의 육질이 더 섬세하고 촘촘하여 지방질이 적고 맛이 좋다는 것을 알 수 있었다. 따라서 PMT에 의한 조직 육질 측정은 생화학적 상태변화를 측정하는 새로운 분석법의 하나가 될 것으로 기대되어진다. Effect of chitosan with sirloin specimens from chitosan-fed and normal-fed pig. The specimens were observed by a optical microscope and their biophoton was measured by a photomultiplier(PMT)tube. Results of experiments showed that specimens of the chitosan-fed pig had lower biophoton rate than that of the normal-fed pig. As a result of the organization were also shown pictures Chitosan as a pork loin tissue and fat in the flesh less delicate and compactness is found that tastes good. Thus by PMT fleshy tissue biochemical measurements to measure the change of state to be one of the new method is to be expected.

마그네타이트를 이용한 selenite와 selenate의 분리

민제호(Je Ho Min),김승수(Seung Soo Kim),백민훈(Min Hoon Baik),배기서(Kie Seo Bae) 한국분석과학회 2011 분석과학 Vol.24 No.4

KURT 지하수 조건에서 셀레늄과 테크네튬의 용해도 및 주요 화학종

김승수,민제호,백민훈,김계남,Kim, Seung-Soo,Min, Je-Ho,Baik, Min-Hoon,Kim, Gye-Nam 한국방사성폐기물학회 2012 방사성폐기물학회지 Vol.10 No.1

장수명 핵분열생성물인 $^{79}Se$와 $^{99}Tc$는 자연수 중에서 용해도가 클 뿐더러 음이온으로 존재하여 방사성폐기물 처분장에서 주요 관심핵종들로 고려되고 있다. 본 연구에서는 KURT 지하수의 다양한 pH와 산화-환원 조건에서 셀레늄과 테크네튬의 Solubility Limiting Solid Phase (SLSP)로 알려진 $FeSe_2$와 $TcO_2$의 용해도를 측정하였다. 또한, 지화학코드를 이용하여 실험과 유사조건에서 이들의 용해도와 주요 화학종을 계산하였다. 실험 및 계산으로부터 pH 8~9.5와 Eh=-0.3~-0.4 V 조건에서 $FeSe_2$의 용해도는 $1{\times}10^{-6}mol/L$이하이며, 주 용해 화학종은 HSe-로 판단된다. $TcO_2(s)$의 경우는 pH 6~9.5와 Eh<-0.1 V 영역에서 용해도와 주 용해 화학종이 각각 $5{\times}10^{-8}{\sim}1{\times}10^{-9}mol/L$와 $TcO(OH)_2$로 나타났지만, Eh=-0.35 V조건에서는 주 용해화학종이 pH가 10.5~12와 12이상에서 각각 $TcO(OH)_3^-$와 $TcO_4^-$로 계산되었다. The long-lived fission products $^{79}Se$ and $^{99}Tc$ have been considered as the major concern nuclides for the disposal of radioactive waste because of their high solubilities and the existence of anionic species in natural water. In this study, the solubilities of $FeSe_2(s)$ and $TcO_2(s)$, known as respective Solubility Limiting Solid Phase (SLSP) of selenium and technetium, were measured in the KURT (KAERI Underground Research Tunnel) groundwater under various pH and redox conditions. And their solubilities and major species were also calculated using geochemical codes under conditions similar to experimental solutions. Experimental results and calculation for $FeSe_2$ show that the solubility of selenium was found to be below $1{\times}10^{-6}mol/L$ under the condition of pH 8~9.5 and Eh=-0.3~-0.4 V while the dominant species was identified as $HSe^-$. For $TcO_2$, the solubility of technetium was found to be $5{\times}10^{-8}{\sim}1{\times}10^{-9}mol/L$ in the solutions of pH 6~9.5 and Eh<-0.1 V, while the dominant species was $TcO(OH)_2$. However, when the Eh of the solution is -0.35 V, $TcO(OH)_3^-$ and $TcO_4^-$ are calculated as the dominant species at pH 10.5~12 and pH>12, respectively.

뽕잎 차에서의 성분 비파괴형 기기 기술 개발 및 응용

천병수,민제호,Cheun, Byeung-Soo,Min, Je-Ho 한국전자통신학회 2013 한국전자통신학회 논문지 Vol.8 No.10

기존의 기기분석에 있어서의 미비한 점을 보완한 물질 분석 과정에서 주요 성분의 파괴를 최소화하고져 비파괴형 원적외선 도자기 가마를 이용하여 비파괴형 성분물질을 유지할 수 있는 기기 분석 장치를 개발 하였다. 또한 광폭증폭관(PMT) 기기를 사용하여 뽕잎 차에 함유된 항산화 물질의 형태적 효과를 기존의 기기분석기와 원적외선 도자기 기기를 사용한 것과의 차별화 실험을 통해 그 효과를 측정 하였다. 그 결과 특히 과산화수소를 이용한 항산화 효과를 측정한 결과 성분이 파괴되지 않음을 측정하여 그 효과가 인정 되었기에 이를 보고한다. In the present study,effect of natural mulbelly leaf tea on DMEM MDCK cell line. Mulbelly leaf teas inhibited the proliferation in primery MDCK cells in a dose dependent manner. These results show that mulbelly teas potent inhibits the reactive oxygen species (ROS) and not destruction a component. Therefore, mulbelly teas might improve overall quality of the color and taste. And might be applied newly to development of componant mulbelly teas quality and biochemical change by ROS in living things.

고등학생의 스포츠 활동정도와 삶의 질의 관계분석

박진경(Park, Jin-Kyung),전호문(Jun-Ho-Moon),민제호(Min, Je-Ho),나순복(Na, Soon-Bok) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.2

This study used data from 292 high school students ultimately out of the convenience sampling of 300 students from high schools educating 1,000 or more students located in the Incheon Metropolitan City in 2013 to analyze the relationship between degrees of sport activities and life quality of high school students. The research tool was a questionnaire, data were processed with the PASW 21.0 program, the hypothesis verification was performed at p<.05 with x², t-test, and ANOVA, and Scheffe-test was employed for post-test. The results from the analysis are as follows. First, as for degrees of sport activities due to the subjects’ general characteristics, differences were found in number of weekly participation, time spent for 1 session, duration of participation between genders, and in number of weekly participation and duration of participation among graders. That is to say, as for genders, male students engaged themselves more in sport activities than their female counterparts, and as for graders, the degree of sport activity participation was higher in second, first, and third graders in that order. Second, as for life quality based on general characteristics with respect to genders, the male students had high life quality in the areas of physical health and social relation, and the female students in the environmental and psychological areas, while the second graders among other graders showed significant differences as they had high life quality in the areas of physical health, social relation, and psychology except for the environmental area. Third, as a result of the analysis of the relationship between sport activities and life quality, there turned out to be significant differences in that the students recognized their life quality as high when the number of participation exceeded 4 times, the time spent for a session was 1~less than 2 hours, and as for the duration of participation, they participated in the physical area for 5 or more years, in the environmental area for 1~less than 3 years, and in the social and psychological areas for 3~less than 5 years. Thus, adolescents’ life quality can be promoted if sustainable long-term sport environments are created in terms of novel sport programs to which adolescents can approach with joy and interest in their everyday life.

피부관리를 위한 전류기기 접근에 대한 고찰

김동순(Dong Soon Kim),김보은(Bo Eun Kim),민제호(je ho Min),김유숙(Yu Suk Kim),최성우(Seong Woo Choi) 대체의학회 2014 대체의학회논문지 Vol.3 No.2

이 연구는 깨풀이 구강암 세포주에 어떤 영향을 미치는지 알아보았다. 구강암 세포주의 KB세포에 깨풀의 열수추출물과 메탄올 추출물을 주사 후 세포의 사멸을 조사하였다. 실험은 MTS 분석과 Western Blot 분석으로 실시하였다. 실험 결과는 깨풀의 열수추출물은 세포의 사멸에 아무런 영향이 없었다. 그러나 메탄올 추출물은 세포가 사멸하는 영향을 보였다. 메탄올 추출물 농도 50, 100, 125, 150, 200, 250, 500㎍ / ㎖에서 24시간 후 95%, 57%, 53%, 52%, 30%, 28%, 9%, 48시간 후 87%, 51%, 35%, 32%, 25%, 15%, 5%, 72 시간 후 72%, 48%, 29%, 20%, 12%, 10%, 4%의 세포 생존율을 보였다. 본 연구 결과 깨풀의 메탄올 추출물이 구강암 치료제로 그 가능성을 보였다. 깨풀의 메탄올 추출물은 암치료을 위한 화학요법보다 훨씬 적은 부작용을 갖기 때문에 이 연구는 구강암 치료의 개발에 많은 중요성을 갖는다고 사료된다. This research conducts how Acalypha Australis effects an oral cancer cell line. A result of cell death was occurring after an injection of hot water extract and methanol extract of Acalypha Australis to the KB Cell in an oral cancel cell line. These cell death analysis were conducted by MTS Assay, Western Blot Analysis. In results, hot water extract of Acalypha Australis had no influence in cell deaths. However, the methanol extract did. It was disposed in concentrated amounts of 50, 100, 125, 150, 200, 250, 500㎍/㎖. The results of the cancer cell deaths were as follows: after 24 hours - 95%, 57%, 53%, 52%, 30%, 28%, 9%, after48 hours - 87%, 51%, 35%, 32%, 25%, 15%, 5%, and after 72 hours - 72%, 48%, 29%, 20%, 12%, 10%, 4%. This study suggests that the methanol extract of Acalypha Australis can control the possibilities of curing oral cancer. This significant study holds much importance in a development of oral cancer treatment because the methanol extract of Acalypha Australis has much less side effects than chemotherapy for cancer treatment.