內科領域에 있어서의 Steroid 療法

閔獸基 中央醫學社 1961 中央醫學 Vol.1 No.1

유방조직에서 티로신 활성효소 수용체인 EphA2, c-met 및 c-erbB-2의 발현

민수기,조현득,조성진,박혜림,신형식,박영의,염범우 대한병리학회 2005 Journal of Pathology and Translational Medicine Vol.39 No.1

Background : Tyrosine kinase receptor (TKR) is an important protein for normal-development, growth and tumorigenesis in human tissues. The purpose of this study was to evaluate the effect of TKR in the progression of breast cancer. Methods : The expressions of EphA2, c-met and c-erbB-2 were examined, by using immunohistochemical methods and RT-PCR, in samples of breast tissue that included 111 samples of normal epithelium, 34 samples of ductal carcinoma in situ (DCIS), and 109 samples of invasive ductal carcinomas (IDC). The results were compared with the prognostic parameters of breast cancer including the tumor grade, growth pattern, lymph node metastasis and the expressions of ER, PR, p53 and Ki-67. Results : The protein expressions of the three TKRs were higher in DCIS and IDC than in normal epithelium. The protein expression of EphA2 was correlated with a tumor grade, a labeling index of Ki-67, and the protein expression of c-met. Overexpression of c-erbB-2 was correlated with lymph node metastasis. The mRNA levels of the three TKRs were correlated with each other in normal tissue and IDC. The level of c-met mRNA was higher in the low grade tumors. Conclusions : The three TKRs may play roles in the tumorigenesis of human breast cancer. The overexpressions of EphA2 and c-erbB-2 may be a poor prognostic parameter in breast cancers.

Expression of HAT1 and HDAC1, 2, 3 in Diffuse Large B-Cell Lymphomas, Peripheral T-Cell Lymphomas, and NK/T-Cell Lymphomas

민수기,조성진,고영호,박윤웅,김효정,서진원,박혜림,김인선 대한병리학회 2012 Journal of Pathology and Translational Medicine Vol.46 No.2

Background: It has generally been proven that histone acetylation and deacetylation are involved in the malignant transformation. To date, however, this has rarely been studied in cases of malignant lymphoma. Methods: We studied nine cases of reactive lymphoid hyperplasia, 78 cases of diffuse large B-cell lymphoma (DLBCL), 13 cases of peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS), and 13 cases of extranodal NK/T-cell lymphoma, nasal type (NKTCL). Thus, we attempted to elucidate the associations of the degree of the expression of histone acetyltransferase 1 (HAT1), histone deacetylase (HDAC) 1, HDAC2, and HDAC3 with the clinical behaviors of above malignant lymphomas using the immunohistochemistry and a western blot analysis. Results: The degree of the expression of HAT1 was higher in cases of DLBCL, PTCL-NOS or NKTCL as compared with reactive lymphoid hyperplasia (p<0.05). The degree of the expression of HAT1 was correlated with that of HDAC1 in cases of DLBCL or NKTCL (p<0.05). The degree of the expression of HAT1 and HDAC1 was correlated with a poor survival in cases of DLBCL or PTCL-NOS (p>0.05). Conclusions: HAT1, HDAC1, and HDAC2 play a critical role in the development of malignant lymphomas. Both HAT1 and HDAC1 might be indicators for a poor prognosis in cases of DLBCL as cooperating factors.

갑상샘 세침흡인세포검사에서 미결정 범주의 분석

민수기,서진원,전선영,민광선,박혜림 대한병리학회 2010 Journal of Pathology and Translational Medicine Vol.44 No.6

Background : Descriptive diagnosis reports have been causing some problems in communication. Our institution decided to use the category diagnosis system since June, 2008. So we evaluated the effectiveness of this change. Methods : The category system is composed of unsatisfactory, suboptimal for diagnosis but suggestive of, most probably benign, indeterminate, suspicious for malignancy and malignancy. We evaluated 1,525 cases from June, 2008 to September, 2009. We analyzed 159 cases of the indeterminate category. Results : Among the 159 cases, 21 were re-aspirated and 63 underwent an operation. The diagnoses of the re-aspirated cases were 2 positive for malignancy, 5 indeterminate, 13 most probably benign, and 1 unsatisfactory. The surgical diagnoses were 39 malignancies, 2 follicular adenomas and 1 Hürthle cell adenoma, and 21 benign lesions. Re-aspiration for the indeterminate cases could help decide whether the lesions need to be operated or not at above 70%. The indeterminate category could predict the surgical diagnosis of the thyroid nodule with statistical significance when the subcategories were indicated (p < 0.001). Conclusions : The category diagnosis is replacing the descriptive diagnosis for the thyroid fine needle aspiration. The indeterminate category is useful and effective for making the decision to operate and especially when the indeterminate subcategories are used.

유방에 발생한 화생성 암종의 세침흡인 세포학적 소견 - 1증례 보고 -

민수기,차희정,김준미,주영채,Min, Soo-Kee,Cha, Hee-Jeung,Kim, Joon-Mee,Chu, Young-Chae 대한세포병리학회 1996 대한세포병리학회지 Vol.7 No.2

Metaplastic carcinoma of the breast is a morphologically heterogenous group of neoplasms characterized by ductal adenocarcinoma with extensive squamous differentiation, a spindle-cell pattern of growth, and/or heterologous mesenchymal elements. We experienced a case of metaplastic carcinoma diagnosed by fine needle aspiration(FNA) and confirmed by radical mastectomy in a 46 year-old woman. The FNA cytologic findings included atypical squamous cells with kertinization tying singly and in clusters in a necrotic background. In addition, scattered spindle cells with pleomorphic large nuclei and prominent nucleoli were present in a hemorrhagic and necrotic background. The histopathologic findings showed moderately differentiated squamous cell carcinoma and highly pleomorphic sarcoma with chondroid component. The immunohistochemical stain revealed focal positive reaction for cytokeratin as well as diffuse reactivity for vimentin in the sarcomatous area.

장(腸)에 발생한 혈관 이형성증

민수기,차희정,김준미,주영채 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.12

Expression of Histone Deacetylases HDAC1, HDAC2, HDAC3, and HDAC6 in Invasive Ductal Carcinomas of the Breast

서진원,민수기,박혜림,김동훈,권미정,김이수,주영수 한국유방암학회 2014 Journal of breast cancer Vol.17 No.4

Purpose: DNA deacetylation by histone deacetylase (HDAC) isan important mechanism involved in the oncogenic tumorigenesisof breast cancer. Previous studies have reported an associationof the estrogen receptor (ER) with HDACs and demonstratedthe efficacy of HDAC inhibitors for the treatment of breastcancers via in vitro experiments. In this study, we examined theassociation of HDAC expression with clinicopathological parametersand disease-specific survival. Methods: Immunohistochemical(IHC) analysis of HDAC1, HDAC2, HDAC3, and HDAC6was performed using tissue microarrays in 300 invasive ductalcarcinomas. IHC scoring was determined by multiplication of theintensity (0 to 3) and the proportion (0 to 4) of staining, and weclassified tumors into low- and high-HDAC expression groups. Results: High expression of HDAC1 was correlated with the molecularsubtype (p=0.001) and human epidermal growth factor 2(HER2) amplification (p=0.012). High expression of HDAC6 wascorrelated with a younger age (p<0.001), ER expression (p=0.025), progesterone receptor expression (p=0.034), molecularsubtype (p=0.023), and HER2 amplification (p=0.011). HighHDAC1 expression was correlated with luminal A tumors (p=0.001), while high HDAC6 expression was more common in luminalB tumors (p=0.023). Although the expression of HDACsdid not exhibit prognostic significance in the entire cohort, highexpression of HDAC1 and HDAC6 was associated with improvedoverall survival (OS) in patients with ER-positive tumors(p=0.017 and p=0.029, respectively), and high expression ofHDAC2 was correlated with improved OS in ER-negative tumors(p=0.048) on univariate analysis. Furthermore, high HDAC6 expressionwas associated with improved disease-free survival (p=0.048) on multivariate analysis. Conclusion: HDAC1 expression issignificantly correlated with the molecular subtypes of tumors,with the highest expression being observed in luminal A tumors. HDAC6 is a significantly correlated with ER expression and themolecular subtype, thereby supporting the estrogen regulatoryproperty of HDAC6. HDAC1 and HDAC6 expression are goodprognostic factors for ER-positive tumors.

투명 세포 수막종

차희정,민수기,김준미,주영채 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.8

갑상선의 미만성 경화성 변종 유두상 암종의 세침흡인 세포학적 소견 - 1예 보고 -

김준미,민수기,주영채,김미림,김경래,Kim, Joon-Mee,Min, Soo-Kee,Chu, Young-Chae,Kim, Mi-Rim,Kim, Kyung-Rae 대한세포병리학회 2000 대한세포병리학회지 Vol.11 No.1

Diffuse sclerosing papillary carcinoma(DSPC), a variant of papillary carcinoma of the thyroid, is characterized by diffuse involvement of one or both thyroid lobes, and histologic features such as prominent sclerosis, intense lymphocytic infiltrate, numerous psammoma bodies, and squamous metaplasia together with the characteristic cytoarchitectural pattern of classical papillary carcinoma. We experienced a case of fine needle aspiration cytologic(FNAC) findings of DSPC, which was confirmed by histologic examination of the thyroidectomy specimens. The patient was 26 years old female who presented with diffuse firm enlargement of the thyroid gland with enlargement of many cervical lymph nodes. FNAC smears showed numerous psammoma bodies, many lymphocytes, metaplastic squamous cells, absence of stringy colloid, and epithelial cells showing classical features of papillary carcinoma, such as nuclear grooves, intranuclear unclusions, and ground glass chromatin pattern.

경막하삼출물과 경막염증이 관찰된 미세다발혈관염 1예

주민경,민수기,유경호,마효일,김윤중,김주용,윤성희,김철호,이병철 대한신경과학회 2005 대한신경과학회지 Vol.23 No.6

Microcopic polyangiitis (MPA) is a systemic disorder affecting small vessels. In MPA, the central nervous system involvements have rarely been reported. We experienced a case of MPA with subdural effusion and pachymeningitis in a 50-year-old woman. The diagnosis of MPA was made by the presence of mononeuritis multiplex, hematuria, pachymeningitis, and p-ANCA seropositivity in this patient. Subdural effusion was of exudates and an active suppurative inflammation was observed on the dural biopsy.