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Comparison of Developmental Efficiency of Murine Somatic Cell Nuclear Transfer Protocol
문정현,노상호,정미란 사단법인 한국동물생명공학회 2017 한국동물생명공학회지 Vol.32 No.3
The Somatic cell nuclear transfer (SCNT) method can be applied to various fields such as species conservation, regenerative medicine, farming industries and drug production. However, the efficiency using SCNT is very low for many reasons. One of the troubles of SCNT is that it is highly dependent on the researcher’s competence. For that reason, four somatic cell nuclear injection methods were compared to evaluate the effect of hole-sealing process and existence of cytochalasin B (CB) on efficiency of murine SCNT protocol. As a results, the microinjection with the hole-sealing process, the oocyte plasma membrane is inhaled with injection pipette, in HCZB with CB was presented to be the most efficient for the reconstructed in SCNT process. In addition, we demonstrated that the oocytes manipulated in Hepes-CZB medium (HCZB) with CB does not affect the developmental rate and the morphology of the blastocyst during the pre-implantation stage. For this reason, we suggest the microinjection involving hole-sealing in HCZB with CB could improve SCNT process efficiency.
문정현 한국건축친환경설비학회 2019 한국건축친환경설비학회 논문집 Vol.13 No.1
In this paper, U-value variations of a fixed window were analyzed qualitatively and quantitatively. The factors which influence the U-value variation of a window when the window size changes are the ratio of the frame U-value to the glass U-value and the ratio of the frame size to the window size. The ratio of the frame size to the window size increases when the window size increases and the frame width is fixed. In this study, when the window size varies and the frame width is fixed, the effect of the ratio of the frame U-value to the glass U-value on the U-value variations of the window were investigated. The calculation of window U-value consists of the u-value calculation for the glass and frame and that at glass edge. When the window size varies, the U-value variation for the glass and frame and that of the edge of glass should be investigated together. When the ratio of the frame U-value to the glass U-value is bigger than 1 and the window size decreases, the U-value for the glass and frame increases against that of the standard test window. The U-value for the edge of glass increases when the window size decreases from that of the standard test widow. Therefore, if the frame U-value is bigger than the glass U-value and the window size is smaller than the standard test case, the U-value difference of the glass and frame and that of the edge of the glass from that of the standard test window are piled up together.
해수열펌프-해수온도차발전 복합플랜트의 P2H 섹터커플링 적용성 분석
문정현,지호,이호생 한국동력기계공학회 2023 동력시스템공학회지 Vol.27 No.6
In the Paris Climate Agreement, all member countries, regardless of developed or developing countries, agreed to implement mandatory greenhouse gas emissions reductions from 2020, and each country is increasing the share of renewable power generation to reduce greenhouse gas emissions. As the spread of renewable power generation increases, various problems such as system instability due to volatility and intermittency and curtailments of renewable power generation are occuring. So many studies are being conducted to overcome these problems by applying sector coupling technology. In this study, a SWHP (SeaWater Heat Pump) - OTEC (Ocean Thermal Energy Conversion) complex system was designed. when surplus power from renewable power generation was supplied to the complex system, the power and costs required for summer cooling and winter heating were compared with electric heat pump (EHP) cooling and heating. In addition, the power that can be supplied from OTEC using stored cold and hotwater as a heat source and heat sink was calculated during peak.
일제강점기 배경의 영화 소비구조 분석-‘모에’와 ‘나쁜 장소’를 중심으로-
문정현 한양대학교 현대영화연구소 2018 현대영화연구 Vol.14 No.1
The films dating back to the Japanese colonial period, which were unveiled since the mid-2010s, are an unprecedented cultural phenomenon. This study attempted to grasp the common feature inherent in a series of films by taking advantage of the database consumption theory that Hiroshi Azuma devised to analyze subculture. As a result, the process of accumulating Moe-elements in the database of subculture in Japan could be acquired in Korean movie contents as well. In addition, the aesthetic arguments of Sawaragi noi were complemented by the former in order to distinguish the structure in which each movie is appealed to the public. Consequently, the paper was able to analyze how Kyungsung as ‘bad place’ invoked organically with Moe-elements. The theoretical discourse of Hiroki Azuma and Sawaragi Noi had a role of analytical tool in this paper. The combination of the two theories served as a major criterion to explain the consumption structure as the setting for various films during the Japanese imperialism period. As a result the paper analyzes post-modern aspects of characters in modern culture and aims to reveal the implications of pseudo - realism in urban space. 2010년대 중반부터 동시다발적으로 개봉한 일제강점기 배경의 영화들은 전례 없는 문화현상으로서 주요한 연구대상이다. 본 연구는 아즈마 히로키가 서브컬처를 분석하기 위해 고안한 데이터베이스 소비이론을 차용해서 일련의 영화들에 내재된 공동분모를 파악하고자 시도했다. 그 결과 일본에서의 하위문화가 작동하는 유사한 방식으로 모에 요소들이 데이터베이스에 축적 및 재배열되는 과정을 국내 영화콘텐츠에서도 포착할 수 있었다. 또한 각 영화들이 대중들에게 소구되는 구조를 파악하기 위해서 사와라기 노이의 미학적 논증들을 전자에 보완했다. 그 결과 나쁜 장소로서 성립하는 역사 속 경성이 모에 요소들과 어떻게 유기적으로 결합하며 호출되는지 분석할 수 있었다. 근대기까지 작품의 배후로 커다란 이야기가 자리했다면 그 이후에는 캐릭터의 중요성이 증가하며 데이터베이스 소비구조가 새롭게 부상했다. 선형적 역사로부터 유리된 나쁜 장소의 지각은 이와 같이 커다란 이야기를 산출하는 문화형식의 반성적 성찰을 유도한다는 점에서 시사적이다. 이처럼 아즈마 히로키와 사와라기 노이가 전개한 담론은 본고의 문제제기를 위한 주요한 분석도구로 인용되었다. 그 결과 영화 속 등장하는 다양한 이미지의 유형을 데이터베이스에 집적되는 모에 요소들로, 도시공간의 지형학에 내재된 분열적 양상들을 상대항으로 정리할 수 있었다. 일제강점기가 다양한 영화 작품들의 배경으로 차용되는 소비구조를 밝히기 위해서 두 이론의 결합은 주요한 비평적 근거로 작용했다. 이와 같이 본 논문은 현대문화에서 성립하는 캐릭터의 포스트모던한 양상을 분석하는 동시에 도시공간의 의사 리얼리즘에 함의된 의미까지 밝히는 것을 목적으로 삼는다.
문정현,심재성,서병기 한국잔디학회 2002 한국잔디학회지 Vol.16 No.2
This study showed the current plantation of woody landscape plants of 42 school gardens in Daejeon metropolitan city. The ratio between evergreen tree and deciduous tree was 67% to 33%. The ratio between evergreen shrub and deciduous shrub was 48% to 52%. Also, 161 kinds of woody species were found; evergreen tree was 21 species, deciduous tree was 67 species, evergreen shrub was 16 species, deciduous shrub was 44 species, and vine and the others were 13 species. The proportion of native woody species was 61% (98 out of 161 species). 110 species were planted at the front garden area. In the mid-garden, 77 kinds of woody species were found. In the side garden, 95 kinds of woody species were found. In the rear garden, 92 kinds of woody species were found. The surrounding of play ground and outer area were planted 82 species. The first ranked evergreen tree was Juniperus chinensis‘Kaizuka’. And Deciduous tree, evergreen shrub, deciduous shrub, and vine showed Gingko biloba, Buxus microphylla var. koreana, Hibiscus syriacus, Wisteria floribunda, respectively. The school symbol of tree and flower was planted in 41 schools. The first rank plants of school symbol tree and flower were Pinus densiflora, Forsythia koreana and Rosa spp. Plant nameplate was found at 22 schools. The plant nameplate was used for 1∼10 species at 17 schools. In conclusion, plantation of landscape trees should be considered characteristic of each school area. In addition, school landscape should be planned distinction of a elementary school, a middle school, a high school, and a school for handicapped. School landscape should be considered carefully from functional, natural, educational environment and maintenance viewpoints as well as urban forest networking.