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마정인,강선혜,정형복,이제희 한국수산과학회 2018 Fisheries and Aquatic Sciences Vol.21 No.1
Stimulator of interferon gene (STING) is induced by various inflammatory agents, such as lipopolysaccharide and microbial pathogens, including virus and bacteria. In this study, we obtained a full-length cDNA of a STING homolog from olive flounder using rapid amplification of cDNA ends PCR technique. The full-length cDNA of Paralichthys olivaceus STING (PoSTING) was 1442 bp in length and contained a 1209-bp open reading frame that translated into 402 amino acids. The theoretical molecular mass of the predicted protein sequence was 45.09 kDa. In the PoSTING protein, three transmembrane domains and the STING superfamily domain were identified as characteristic features. Quantitative real-time PCR revealed that PoSTING expressed in all the tissues analyzed, but showed the highest level in the spleen. Temporal expression analysis examined the significantly upregulated expression of PoSTING mRNA after viral hemorrhagic septicemia virus (VHSV) stimulation. In contrast, no significant changes in the PoSTING expression were detected in Edwardsiella tarda-challenged group compared to the un-injected control. The expression of P. olivaceus type I interferon (PoIFN-I) was also highly upregulated upon VHSV challenge. These results suggest that STING might be involved in the essential immune defense against viral infection together with the activation of IFN-I in olive flounder.
졸 - 겔법으로 제조한 알루미나막의 기체 투과 및 분리 특성
정인재,마명근,권홍섭,마상국 한국화학공학회 1991 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.29 No.5
알루미늄 이소 프로폭사이드로부터 산성 조건에서 졸-겔법에 의하여 보헤마이트 졸을 만들어 알루미나 지지체 위에 코팅하였다. 이것을 상온에서 이틀간 건조후 500℃에서 한 시간동안 열처리하였다. 이 막은 약 3 ㎚ 매우 작고 균일한 기공을 가지며, 코팅의 횟수에 따라 0.5-8.2 ㎛의 두께를 가졌다. 7.5 ㎛의 두께를 가진 막의 기체 투과 특성을 헬륨, 질소, 수소 그리고 이산화탄소에 대하여 조사하였다. 이들 기체에 대해 Knudsen 확산에 의한 투과가 지배적이었다. 그러나 이산화탄소와 질소의 경우 표면 확산에 의한 영향으로 Knudsen확산에 의하여 예측되는 값보다 상당히 높은 투과도를 나타냈다. 흡착 등온선으로부터 얻어진 이산화탄소의 흡착 에너지는 22.40kJ/㏖이었으며, 290 K에서 계산된 이산화탄소와 질소의 표면 확산계수는 각각 10.66×10^(-9)㎡/sec와 10.84×10^(-9)㎡/sec 였다. 표면 확산에 의한 추가적인 흐름량은 이산화탄소와 질소에 대하여 각각 43%와 22%였다. 이산화탄소-수소, 이산화탄소-질소 혼합물의 분리인자는 온도 증가에 따라 Knudsen 확산에 의한 이론비에 접근하였다. Boehmite AlO(OH) sol was prepared by sol-gel method from aluminum iso-propoxide under an acidic condition and this sol was coated on an alumina support. After it was dried for 2 days at room temperature and calcined at 500℃ for one hour. The membrane had homogeneous ultra fine pores, about 3.0 ㎚, with a narrow pore size distribution and the thickness was in the range of 0.5-8.2 ㎛ depending upon the number of coatings. Gas permeation characteristics of the membrane with 7.5㎛ thickness were investigated for four gases He, N₂, H₂ and CO₂. Knudsen diffusion was the dominant transport mechanism for these gases. But CO₂ and N₂ showed much higher permeabilities than the values predicted by Knudsen diffusion due to the effect of surface diffusion. The adsorption energy of CO₂ obtained from adsorption isotherms was 22.40kJ/㏖ and the calculated surface diffusion coefficients of CO₂ and N₂ turned out to be 10.66×10^(-9) ㎡/sec and 10.84×10^(-9) ㎡/sec at 290 K, respectively. The additional flows by surface diffusion of CO₂ and N₂ was about 43% and 22% of the total flows, respectively. The separation factors of CO₂-H₂ and CO₂-N₂ mixtures approached the theoretical values predicted by Knudsen diffusion with increasing temperature.
이종민,마상동,한태룡,김공환,임상빈,정인식 경희대학교 생명자원과학연구원 1997 遺傳工學論文集 Vol.9 No.-
Supercritical fluids (SCF) are useful substances that are recently used in bioseparation process. Extraction of carthamin from safflower using supercritical carbon dioxide were examined at various conditions of temperature (35∼40℃), pressure (3000∼5000 psi) and CO₂ flow rate (900∼1200 m/hr). SCF was less effective than solvent methods in carthamin extraction from safflower. Concentration of carthamin was 50% more efficent at the use of alginate beads with 35 g cellulose per column loadings compared to the control (15g cellulose per column loadings).