젖소의 산유수준과 자동착유시스템간의 상관관계 분석

남인식,장경만,손용석,기광석,정하연,강희설,박성민,Nam, In-Sik,Chang, Kyeong-Man,Son, Yong-Suk,Ki, Kwang-Seok,Jung, Ha-Yeon,Kang, Hee-Seol,Park, Seong-Min 한국축산환경학회 2015 한국축산시설환경학회지 Vol.21 No.2

Total three hundred and fourteen dairy cows were used in this study to analysis of correlation between milking levels and automatic milking system (AMS). Cows were divided into five groups according to their milking levels (over 50 kg/day, 49~40 kg/day, 39~30 kg/day, 29~20 kg/day, 19~10 kg/day). All groups were compared with daily milking number, rejected number by AMS, residence time at AMS, milking time, flow rate of milk. Daily milking number and milking time were higher in the group of over 50 kg and 49~40 kg than that of other groups (p<0.05). There are no correlation on rejected number by AMS, residence time at AMS and flow rate of milk between milking levels and AMS. In addition, we found that the milk yield affected to feed intake and rumination of dairy cows. In conclusion, present results indicated that installation of AMS on dairy farms with high milk producing cows might be affected positively.

반추위내 서식하는 혼합곰팡이와 박테리아에 의한 Linoleic Acid 가수소화반응과 Stearic Acid 생산에 관한 연구

남인식,Nam, In-Sik 한국미생물학회 2007 미생물학회지 Vol.43 No.2

홀스타인 건유우의 반추위에서 분리한 혼합 곰팡이에 첨가한 linoleic acid가 biohydrogenation 과정 중 생산되는 지방산의 농도와 종류를 측정하고, 최종 산물로 생산되는 지방산이 trans-11 vaccenic acid인지 stearic acid인지 조사하기 위하여 본 연구를 수행하였으며 결과는 다음과 같다. 반추위 혼합 박테리아 배양구에 linoleic acid 용액을 첨가한 결과, 배양 90분 이내에 100%의 linoleic acid가 stearic acid로 biohydrogenation되었다. 반면에 linoleic acid 용액을 반추위 혼합 곰팡이에 첨가한 결과 24시간 이내에 모든linoleic acid는 conjugated linoleic acid (cis-9, trans-11)와 trans-11 vaccenic acid로 biohydrogenation되었다. Linoleic acid가 함유된 혼합곰팡이 처리구는 배양시간이 증가할수록 stearic acid의 농도도 소량 증가하는 경향을 보였다. 또한 linoleic acid가 함유되지 않은 혼합곰팡이 대조구에서도 배양시간이 증가할수록 stearic acid 농도가 처리구와 비슷하게 증가하는 경향을 나타내었다. 그러나 혼합 박테리아의 linoleic acid 첨가구에서는 배양시간이 증가할수록 stearic acid의 농도가 급격하게 증가하는 것을 조사되어 반추위 혼합곰팡이의 stearic acid생산은 linoleic acid의 biohydrogenation과 무관하게 생산되는 것으로 조사되었다. 따라서 반추위 혼합 곰팡이에 의한 linoleic acid biohydrogenation의 최종 산물은 trans-11 vaccenic acid로 판단되며, 혼합 박테리아는 stearic acid로 나타났다. The objective of this study was to confirm biohydrogenation of linoleic acid and stearic acid production by mixed men fungi and bacteria. In mixed fungal biohydrogenation study, when linoleic acid solution was added to fungal culture (after 24 hr pre-incubation), all linoleic acids were converted to trans-11 vaccenic acid via cis-9, trans-11 conjugated linoleic acid production within 24 hr period of incubation. All linoleic acid solution was hydrogenated to trans-11 vaccenic acid within 24 hr incubation and this was continued until the end of incubation (48 hr). Both treatments (added linoleic acid solution or the same amount of solution without containing linoleic acid into fungal cultures) produced the similar amount of stearic acid. In contrast, 100% of linoleic acid solution was hydrogenated to stearic acid in mixed bacterial culture. It is concluded that the end product of mixed fungal biohydrogenation of linoleic acid is trans-11 vaccenic acid whereas mixed bacteria produced stearic acid as an end product of their biohydrogenation.

착유시스템 및 계절이 홀스타인 착유우의 유성분에 미치는 영향

남인식,허병무,박호경,민태홍,손용석,박성민,권응기,장경만,Nam, In-Sik,Heo, Byong-Moo,Park, Ho-Kyung,Min, Tae-Hong,Son, Yong-Suk,Park, Seong-Min,Kwon, Eung-Gi,Chang, Kyeong-Man 한국축산환경학회 2014 한국축산시설환경학회지 Vol.20 No.3

This study was conducted to examine the effect of different types of milking instrument and season on milk composition (fat, protein, non-fat milk solids, milk urea nitrogen and somatic cell count) on Holstein-Friesian lactating cows. Raw milk samples were collected from 20 automatic milking system (AMS) installed dairy farms and 85 conventional milking system (CMS) installed dairy farms. Milk fat, protein, NFMS and MUN contents did not changed between AMS and CMS and different seasons. On the other hand, the average SCC in AMS was $239{\times}10^3cells/mL$ which was lower than in CMS ($373{\times}10^3cells/mL$, (p<0.05). In conclusion, present results indicated that SCC in milk might be influenced by AMS. However, this area needed more study to confirm the reason of reducing SCC content from AMS.

최근 소비트렌드에 맞는 육우산업 발전 방향

남인식,Nam, In-Sik 한국낙농육우협회 2017 낙농·육우 Vol.37 No.4

양계업(제도) 변화와 발전방안 - HACCP 인증제도

남인식,Nam, In-Sik 대한양계협회 2020 월간 양계 Vol.52 No.8

기고 - 자동 착유 농가와 일반 착유 농가에서 생산된 유성분의 차이점 분석

남인식,Nam, In-Sik 한국낙농육우협회 2016 낙농·육우 Vol.36 No.7

반추위에서 분리한 곰팡이의 Conjugated Linoleic Acid 생산과 ITS-1 영역의 염기서열 해석

남인식,Nam, In-Sik 한국미생물학회 2007 미생물학회지 Vol.43 No.2

반추위에서 분리한 곰팡이(IS-13)의 conjugated linoleic acid 생산 과정을 조사하고conjugated linoleic acid를 생산하는 곰팡이를 동정하기 위하여 본 연구를 실시하였다. IS-13 곰팡이가 함유된 배양액에 linoleic acid를 첨가한 결과 linoleic acid는 배양 12시간 이내에 conjugated linoleic acid와 trans-11 vaccenic acid로 biohydrogenation되었다. IS-13 곰팡이의 동정은 internal transcribed spacer 1 영역(ITS1)을 sequence하여 GenBank 유래의 관련 23속(종)의 반추위 곰팡이와 비교하였다. IS-13 곰팡이의 ITS1의 length는 218 bp인 것으로 확인되었으며, 염기서열 분석 결과 Orpinomyces species와 98% 일치하여 반추위내 Orpinomyces species는 conjugated linoleic acid 생산에 깊이 관여하는 것으로 확인되었다. 연구결과 본 균주는 Orpinomyces 속에 속하는 균으로 동정되었다. The aim of this study was to isolate and identify men fungus which produces conjugated linoleic acid. IS-13 fungus hydrogenated conjugated linoleic acid and trans-11 vaccenic acid within 12 hr after addition of linoleic acid. The homology of IS-13 rumen fungus was compared with internal transcribed spacer 1 region (ITS1)sequences of twenty three men fungi. The length of ITS1 region of IS-13 isolate was 218 bp. IS-13 isolate has the most similar sequence (98% matched) with Orpinomyces species according to maximum-likehood and distance matrix results. The result supported that IS-13 isolate belonged to Orpinomyces genus.

반추기축영양 : 한우 암송아지의 성성숙 전 유지 단백질 요구량 결정

남인식 ( In Sik Nam ),오영균 ( Young Kyoon Oh ),장선식 ( Sun Sik Jang ),김도형 ( Do Hyung Kim ),설용주 ( Yong Joo Seol ),김경훈 ( Kyoung Hoon Kim ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.2

본 연구는 성성숙 전 한우 암송아지의 일일 유지 단백질(CPm) 요구량을 구하기 위하여 평균체중 143.1 kg(실험 1)과 257 kg(실험 2)의 각 6두씩을 3수준의 단백질을 급여하는 two 3×3 latin square design에 공시하였다. 실험 1에서는 기초사료로서 timothy 건초를 2.8 kg/d/head를 급여(LCP)하면서 분쇄옥수수와 corn gluten meal을 각각 250 g과 150 g(MCP) 그리고 500 g과 300 g(HCP) 보충 급여하였다. 실험 2에서는 기초사료로서 timothy 건초를 4.8kg/d/head를 급여(LCP)하면서 분쇄옥수수와 corn gluten meal을 각각 350g과 250g(MCP) 그리고 700g과 500g(HCP) 보충 급여하였다. 실험 1에서의 CP 섭취량은 LCP, MCP, HCP구에서 각각 236.6, 340.1, 459.8g/d 이었고, 대사체중당 조단백질 균형은 각각 0.51, 1.87, 3.20g 이었다. 실험 2에서의 CP 섭취량은 LCP, MCP, HCP구에서 각각 415.2, 606.9, 793.0g/d 이었고, 대사체중당 조단백질 균형은 각각 0.67, 1.03, 2.99g 이었다. 실험 1과 2에서 얻은 CPm 요구량은 각각 4.58과 5.02/BW(0.75) 이었고 한우 사양표준(농림부, 2007)에서 채택하고 있는 5.56g CP/BW0.75 보다 낮았다. Two trials at different body weights of Hanwoo heifers(average body weight of 143 and 257 kg, respectively) were conducted to determine crude protein requirements for maintenance(CPm). Six Hanwoo heifers in each trial were used in two 3×3 Latin square design with three diets containing three levels of CP, 14 days in each period. In trial 1, the diets were based on 2.8 kg fresh wt./day/heifer timothy hay(LCP) with supplements of either 250 g ground corn and 150 g corn gluten meal (MCP) or 500 g ground corn and 300 g corn gluten meal(HCP). In trial 2, the diets were based on 4.8 kg fresh wt./day/heifer timothy hay (LCP) with supplements of either 350 g ground corn and 250 g corn gluten meal(MCP) or 700 g ground corn and 500 g corn gluten meal(HCP). In trial 1, CP intakes were 236.6, 340.1, and 459.8 g/d for LCP, MCP, and HCP, respectively. Crude protein balances were 0.51, 1.87 and 3.20g/BW(0.75)/d for LCP, MCP, and HCP, respectively. In trial 2, CP intakes were 415.2, 606.9 and 793.0g/d for LCP, MCP and HCP, respectively. Crude protein balances were 0.67, 1.03, 2.99g/BW(0.75)/d for LCP, MCP, and HCP, respectively. The maintenance requirements for CP from the regression equation between CP intake and CP balance were 4.58g/BW(0.75)/d(trial 1) and 5.02 g/BW0.75/d(trial 2) and lower than the value(5.56 g/BW0.75/d) adopted by Korean Feeding Standards for Hanwoo (2007).

투과성 해안구조물의 소상파 및 내부수위변동에 관한 수치모델링

남인식(IN-SIK NAM),윤한삼(HAN-SAM YOON),김종욱(JONG-WOOK KIM),류청로(CHEONG-RO RYU) 한국해양공학회 2002 韓國海洋工學會誌 Vol.16 No.5

A numerical model has been developed for the permeable coastal structures to simulate hydraulic characteristics on the permeable slopes, which interact with internal flow field of the structures. The model includes hydraulics in the porous medium. Numerical model was calibrated using hydraulic model experiments performed in 2-D wave flume in the Institute of Ocean Hydraulics in PKNU. Better aggrements were obtained with the model which employed inertia resistance term than with the conventional model, PBREAK.

젖소목장의 자동착유시스템 도입 확대 방안 연구

남인식(In-Sik Nam),장경만(Kyeong-Man Chang),허병무(Byoung-Moo Heo),양영석(Young-Seok Yang),박성민(Seong-Min Park),손용석(Yong-Suk Son),남기택(Ki-Teag Nam) 한국농식품정책학회 2016 농업경영정책연구 Vol.43 No.4

Only 1.5% of dairy farms in Korea is using AMS due to high cost and the limited information about the AMS. In this study, we surveyed an awar-eness and adoption attitude of the AMS. All data were collected from eighty dairy farms there they are using conventional milking systems (CMS). Nearly half of dairy farmers (49 farms) already knows about AMS and almost 20% of CMS farms answered to be heard the AMS. The main reason to adopt AMS was for reducing workforce on dairy farm. However, most of dairy farms have the budget problems to buy AMS. In conclusion, if the Korean government support some budget for AMS adaptation or development of inexpensive AMS, the rate of AMS dairy farm will increase up to 20% like EU country.