성인으로부터 분리된 Lactobacillus casei CU2604의 우유배지에서의 생장 특성 및 생리적 특성

김희진,최재경,이경민,임중현,엄석진,김근배,Kim, Hee-Jin,Choi, Jae-Kyoung,Lee, Kyung-Min,Im, Jung-Hyun,Eom, Seok-Jin,Kim, Geun-Bae 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.5

As a trial for the development of a new starter culture for yogurt products, more than two hundred lactic acid bacteria strains were isolated from raw milk and healthy human feces. The strains that showed excellent growth and acid production ability in the 10% skim milk media were selected and identified as Lactobacillus casei through the API carbohydrate fermentation pattern and 16S rDNA sequence analysis. L. casei CU2604 was further investigated for its physiological characteristics as a starter culture compared with a commercial strain. The CU2604 strain showed good acid production and growth characteristics in milk, which were comparable to those of the L. casei Shirota strain. Despite the fact that both these strains displayed the same sugar fermenting pattern and PFGE band pattern, and had similar growth characteristic in milk, L. casei CU2604 exhibited different fatty acid composition in the cell wall, showed more tolerance to bile and to pH, and presented better growth inhibition activity against pathogenic bacteria. Based on these results, the L. casei CU2604 strain holds great promise for use as a novel and efficient starter culture in the production of yogurt. Additional studies on the probiotic characteristics of this strain are currently being conducted.

반회후두신경 손상을 동반하지 않은 갑상선 절제술 환자에서 수술 초기의 음성 및 연하 기능의 변화에 대한 분석

김희진,금보람,김근희,전승식,김혜진,김성균,홍석진,홍석민,김용복,박일석,Kim, Heejin,Keum, Bo-Ram,Kim, Geun Hee,Jeon, Seung Sik,Kim, Hyejeen,Kim, Sung Kyun,Hong, Seok Jin,Hong, Seok-Min,Kim, Yong-Bok,Park, Il-Seok 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : After thyroidectomy, many patients experience problems report such things as reduced voice range and vocal fatigue, swallowing problems without superior and recurrent laryngeal nerve injury. The purpose of this study was to evaluate voice and swallowing problems before and after thyroid surgery without laryngeal nerve injury. Materials and Methods : Ninety-three patients who underwent thyroidectomy without laryngeal nerve injury and completed the follow-up evaluations were studied between June 2013 and December 2015. Each evaluation was performed preoperatively, as well as 1 week, 1 month postoperatively. Analysis was performed including voice handicap index (VHI), dysphagia handicap index (DHI), and acoustic voice analysis. Results : Patients show significant variation of parameters in the fundamental frequency (F), maximal phonation time (MPT), shimmer, jitter and soft phonation index (SPI) early after operation, and most of them showed recovery of parameters after 1month of operation. Perceptive complaint of voice and swallowing also showed significant decreased after operation (p<0.005). After 1month of operation, MPT, highest frequency and frequency ranges still showed significant decreased parameters. Comparing acoustic and perceptive parameters of total thyroidectomy and lobectomy, there was no significant changes between them except highest frequency (p=0.042). Conclusion : The results from both subjective and objective evaluations show voice and swallowing disturbance after thyroidectomy even in the absence of laryngeal nerve and provide patients information about the recovery process after surgery. Highest frequency parameter showed most significant changes after operation.

동물복지를 고려한 점등시간이 육계 병아리의 성장 능력 및 혈액 성상에 미치는 영향

김희진,손지선,전진주,김현수,강환구,이우도,윤연서,홍의철,Kim, Hee-Jin,Son, Jiseon,Jeon, Jin-Joo,Kim, Hyun-Soo,Kang, Hwan-Ku,Lee, Woo-Do,Yun, Yeon-Seo,Hong, Eui-Chul 한국가금학회 2022 韓國家禽學會誌 Vol.49 No.3

This study was conducted to investigate the effect of three different photoperiods on growth performance, blood properties, and stress indicators in broiler chicks between 1-7 days after hatching. Two hundred and fifty-two 1-day-old male broiler chicks (57.0±0.12 g) were divided into three treatments, with 4 replicates per treatment and 22 birds per replicate subjected to three different photoperiods of 24L, 22L/2D and 18L/6D. A light-emitting diode bulb served as the light source, with an illuminance of 30 lx. As an experimental diet, a commercial feed based on a corn-soybean meal, with 22% CP and 3,150 kcal/kg ME diet, and water were fed ad libitum. Body weight gain, feed conversion ratio, and liver weight ratio showed a statistically significant difference between the 18L/6D and 24L treatments (P<0.05), but with no significant difference between the 22L/2D treatment and either the 24L or 18L/6D treatment. The breast meat ratio was 5.59% in the 18L/6D treatment group, which was lower than that of other treatment groups (P<0.05). The triglyceride levels were highest (P<0.05) in the 18L/6D treatment among treatments, but alanine aminotransferase levels were significantly higher (P<0.05) in the 22L/2D treatment than in the 24L treatment. Levels of cytokines, i.e., Interleukin-6 and Tumor Necrosis Factor-α did not show a significant difference among the treatments, but corticosterone content was significantly higher (P<0.05) in the 24L treatment than in the 18L/6D treatment. In conclusion, 22 hours of lighting is appropriate between 1~7 days after hatching, considering growth performance and the overall health of broiler chicks.

Genome Type Analysis of Human Adenoviruses Associated with Pediatric Gastroenteritis in Korea

김희진,양재명,김경희,Kim, Hee-Jin,Yang, Jai-Myung,Kim, Kyung-Hee 대한미생물학회 1996 Journal of Bacteriology and Virology Vol.26 No.1

대략 36,000 base pairs (bp)의 두 가닥짜리 DNA를 지놈으로 가진 사람 아데노바이러스 (Ad)는 DNA 상동성(相同性) 및 생물학적/생화학적 성격이 특이한 49개의 혈청형이 알려져 있는데, 이들 대부분의 Ad가 영유아군 및 면역능이 저하된 성인에서 치사적 결과를 초래할 수 있다. Ad의 세포향성(向性)(tropism)은 매우 다양하여 종류에 따라 상기도 감염, 각결막염, 영유아 장염등을 유발하는데 최근 Ad의 다양한 병원성에 대한 원인을 분자생물학적 수준에서 규명하려는 노력의 일환으로 지역에 따라 주되게 출현하는 Ad형 규명이 활발히 이루어지고 있다. Ad 동정/확인은 표면을 이루고 있는 group 공통항원인 hexon 단백질을 탐지하는 효소면역 측정법 (EIA)에 의하며, Ad형별은 Ad fiber의 세포독성 중화시험에의 한다. 그러나, 세포독성 중화시험이 엄청난 노동력 및 시간을 요구하면서도 민감도/특이도가 만족스럽지 못하여 이를 개선하기 위하여 검체 또는 세포배양에서 Ad DNA를 추출하여 제한효소 절단형태를 비교하는 방법이 개발되었는데 이는 세포배양에 잘 자라지 않는 바이러스주의 형별뿐만 아니라 지역 분리주들의 지놈 변형주를 관찰하는 분자생물학적/분자역학적 연구에도 도움이 되고 있다. 국내에는 Ad와 관련된 소아장염의 빈도가 rotavirus에 의한 것 다음으로 빈번한데도 Ad40/41외에 주되게 출현하는 장내 Ad형들이 전혀 규명된 바 없고, 한국형 Ad들의 지놈형태가 전혀 보고된 바가 없다. 또한 세계적으로 Ad형별 조사지역이 늘어감에 따라 유아장염과 연관된 Ad 역시 Ad40, 41이 외의 형들이 Ad40, 41을 능가하는 것으로 보고되고 있는 지역도 있으나 국내에서는 Ad40, 41이외의 형들은 그 역학적 중요도가 전혀 알려져 있지 않다. 이로서 본 연구의 목적은 Ad주들에 특이 중화항체를 이용한 세포독성 중화시험과 Ad DNA 절단법을 적용하여 한국형 장내 Ad주들의 형별을 처음으로 시도함과 동시에 1989-1991사이 출현한 Ad들의 유전적 변형을 관찰하려는 것이었다. 두 방법 모두 사용하였을 때 주되게 출현하는 장내 Ad형들은 Ad4l, Ad2, Ad7, Ad5, 및 Ad40이었다. Ad40/41-양성 검체를 제외한 Ad hexon-EIA양성들의 77.5%를 형별 할 수 있었던 Ad DNA의 제한효소 절단방법은 형들간의 교차중화로 특이성이 낮았던 중화방법 (47.5%)보다 매우 효율적이어서 두 가지 방법을 함께 적응하였을 때는 40주중의 81.5%인 35주를 형별 할 수 있었다. 또한Ad DNA 제한 효소 절단방법은 Ad7 변이주 (Ad7b)도 탐지 할 수 있었다.

그래핀/구리폼과 그래파이트 하이브리드 구조체의 열전도 특성 연구

김희진,김형근,김예나,이우성,윤대호,양우석,Kim, Hee Jin,Kim, Hyeungkeun,Kim, Yena,Lee, Woo Sung,Yoon, Dae Ho,Yang, Woo Seok 한국결정성장학회 2013 한국결정성장학회지 Vol.23 No.5

그래핀(Graphene)은 전기 전도성 및 열전도성이 우수하고 1 nm 수준의 초 박막 형 필름 소재를 제조할 수 있다는 장점으로 인하여, 차세대 트랜지스터 소자 및 디스플레이 장치에 적용 가능한 방열 소재로서 많은 연구가 활발히 진행되고 있다. 또한 CVD(chemical vapor deposition)제조법으로 합성된 그래파이트(Graphite)는 구조의 단순성 및 유연성 때문에 안정하고 열에 강한 탄소계 방열소재로 주목 받고 있다. 본 연구는 열전도도가 우수한 폼(foam)형태의 구리를 촉매로 상압과 진공에서의 CVD법을 이용하여 그래핀을 성장시킨 후 구리 폼의 기공 안에 다양한 종류의 그래파이트(Natural graphite, expandable(/expanded) graphite, etc)를 복합 및 안정화시켜 기존보다 높은 열전도도를 가지는 방열소재를 개발하였다. 제조된 금속폼/그래파이트 소재를 OM(optical microscopy)과 SEM(scanning electron microscopy)을 이용하여 표면을 확인하였고 DSC(Differential Scanning Calorimetry), 아르키메데스 법을 활용한 비열, 밀도 결과를 확보하였다. 또한 LFA(Laser Flash Analysis)를 이용하여 열 확산계수 예측을 통한 열전도 특성을 평가하였다. Pure-carbon materials such as graphite, graphene, carbon nanotubes, and diamond have very high thermal conductivities. The reported thermal conductivity of graphene is in the range 3000~5000W/m-K at room temperature. Here, we developed graphene/cu foam hybrid type heat spreader to obtain higher thermal conductivity than Cu foam. Hybrid materials were characterized using optical microscopy (OM), scanning electron microscopy (SEM) and thermal conductivity measurement system; LFA (Laser Flash Analysis @ LFA 447, NETZSCH). We suggest that excellent thermal properties of graphene/cu foam hybrid structures are beneficial for all proposed electrical applications and can lead to a thermal management application.

Er:YAG 레이저 조사가 S. mutans의 성장 및 산 생성능에 미치는 영향

김희진,국중기,이상호,이난영,Kim, Hee-Jin,Kook, Joong-Ki,Lee, Sang-Ho,Lee, Nan-Young 대한소아치과학회 2003 大韓小兒齒科學會誌 Vol.30 No.4

치아우식증은 산에 의해 법랑질이 탈회되는 현상으로 소아 및 청소년기에 가장 흔히 발생하는 구강질환 중의 하나로서, 치아우식증의 원인요소 중 다른 인자들보다 객관적으로 조사할 수 있는 구강내 세균에 대한 연구가 활발히 이루어져 왔다. 이 연구는 구강내의 치태라는 고체상태의 조건과 유사한 상태로 Er:YAG 레이저가 구강내 산 생성세균인 S. mutans의 증식에 미치는 영향 및 레이저의 조사시간에 따른 차이를 평가하고자 하였다. Er:YAG 레이저의 구강내 산 생성 세균인 S. mutans에 대한 증식억제효과를 평가하기 위하여 S. mutans pellet에 Er:YAG 레이저를 비접촉식 방법으로, 50mJ, 10Hz, 그리고 조사시간을 1초, 3초, 5초, 7초, 9초로 달리하여 조사하고 이때의 세균의 성장률과 산 생성능을 0, 2, 4, 8, 12, 24, 36, 48시간 동안 측정한 결과 다음과 같은 결과를 얻었다. 1. 레이저를 조사한 군이 레이저를 조사하지 않은 대조군에 비해 세균의 성장률이 감소되었다(P<0.01). 2. 레이저를 조사한 후 12시간까지 1, 3, 5초 조사군에 비해 7, 9초 조사군에서 세균의 성장율이 유의하게 감소되었으나(P<0.05), 24시간 후부터는 서로 유의할만한 차이를 보이지 않았다. 3. 레이저를 조사한 시간이 증가함에 따라 세균의 산 생성능력이 회복되는 시간이 길어짐이 관찰되었고 24시간 후에는 대조군과 차이를 보이지 않음으로써 일정 시간 이후에는 점차적으로 산 생성능력이 회복됨을 알 수 있었다. The purpose of this study was to investigate the inhibitory effect of Er:YAG laser against the intraoral acid producing bacterium of S. mutans. Bacterial pellet containing S. mutans KCTC 3065 was irradiated by Er:YAG laser having a $650\;{\mu}m$ diameter beam by non-contact mode. Irradiated parameters were 50mJ, 10Hz and exposure time were 1s, 3s, 5s, 7s, 9s respectively. We obtained the following results of relative growth rate and acid-producing ability of S. mutans by culturing for 48hrs. 1. The growth rate of S. mutans was decreased in the group of laser irradiation compared to the control group(P<0.01). 2. The growth rate at laser irradiation group of 7s, 9s irradiation time was decreased significantly compared to the laser irradiation group of 1s, 3s, 5s irradiation time, until 12 hours(P<0.05). After 24 hours, all groups of laser irradiation were not found to be statistically different in each other. 3. The acid-producing ability of S. mutans was inhibited for a certain duration by irradiation of laser. In summary, the growth rate and acid producing ability of S. mutans decreased according to laser irradiation. This effect was directly related to the amount of irradiation time. These results suggested that Er:YAG laser had an growth inhibition effect on S. mutans.

IPS Empress 도재관의 파절강도 : 하악 제2소구치에서 교합면 두께와 축면 경사도에 따른 영향

김희진,이해형,남영성,동진근,Kim Hee-Jin,Lee Hae-Hyoung,Nam Young-Sung,Dong Jin-Keun 대한치과보철학회 2002 대한치과보철학회지 Vol.40 No.5

The purpose of this study was compare the fracture strength of the IPS Empress ceramic crown according to the occlusal depth (1.5mm, 2.0mm, 2.5mm) and axial inclination ($4^{\circ},\;8^{\circ},\;12^{\circ}$) of the lower second premolar. After 10 metal dies were made for each group, the IPS Empress ceramic crowns were fabricated and cemented with resin cement. The cemented crowns were mounted on the testing jig with inclination of 30 degrees and the universal testing machine was used to measure the fracture strength. The results of this study were as follows : 1. The fracture strength of the ceramic crown with 2.5mm depth and $12^{\circ}$ inclination was the highest (1284 N). Crowns of 1.5mm depth and $8^{\circ}$ inclination had the lowest strength (951 N). 2. There were no significant differences in the fracture strength by axial inclination of the same occlusal depth group. 3. Most fracture lines began at the loading area and extended through proximal surface perpendicular to the margin, irrespective of occlusal depth.

측두하악관절의 골관절염 치료에서 Avocado/Soybean Unsaponifiables의 효용성

김희진,박준상,고명연,안용우,Kim, Hee-Jin,Park, June-Sang,Ko, Myung-Yun,Ahn, Yong-Woo 대한안면통증구강내과학회 2006 Journal of Oral Medicine and Pain Vol.31 No.2

The aim of this study was to investigate the beneficial effect of a daily dose of 300 mg of ASU taken for more than 3 months on the subjects diagnosed as osteoarthritis of temporomandibular joint by RDC/TMD. Total 68 outpatients(15-54y) of female except menopause in Orofacial Pain Clinics of the Pusan National University Hospital were randomly assigned to either an ASU group(n=36) or a placebo group(n=32). The pain, noise and limited mouth opening(LOM) were evaluated by numerical analogue scale(NAS, range 0-10) and maximum comfortable opening(MCO) were measured by milimeter scale. The difference of simple uptake rate(SUR) on bone scan, hot spot(HS) on coronal SPECT, condylar bony changes on CT between the ASU and placebo groups were compared to investigate the objective effect. The obtained results were as follows. 1. Comparison of the NAS of pain, noise, LOM and MCO before treatment and 3, 6 and 9 months after treatment showed no significant difference between the ASU and placebo groups. 2. Comparison of the NAS of pain, noise, LOM and MCO before treatment and 3, 6 and 9 months after treatment showed no significant difference between the ASU and placebo groups without splint treatment, but showed more increased MCO in the ASU group than the placebo group with splint treatment at 6, 9 months after treatment. 3. Comparison of the NAS of pain before treatment and 3, 6 and 9 months after treatment that the NAS of pain at first visit divided into two groups(above or below 6) showed more decreased the NAS of pain in the ASU group than the placebo group that the NAS of pain at first visit was above 6. 4. Comparison of the NAS of pain, noise, LOM and MCO during 6 months period showed improvement of clinical symptoms within group, but no significant difference between subjects. 5. The simple uptake ratio(SUR) on bone scan and hot spot(HS) on coronal SPECT showed more increased SUR and HS in affected side than non-affected side of the ASU and placebo groups. 6. Comparing of condylar bony changes, osseous remodeling were observed highest, osteophyte lowest in the affected and non-affected side of the two groups. After treatment, comparison of condylar bony changes were observed more decreased erosive features in the ASU group than the placebo group.

신경병증성 통증을 유발한 흰쥐에서 신경손상부위에 따른 배근신경절 및 척수의 신경전달물질의 변동

김희진,김우경,백광세,강복순,Kim Hee-Jin,Kim Woo-Kyung,Paik Kwang-Se,Kang Bok-Soon 대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.3

Peripheral nerve injury sometimes leads to neuropathic pain and depletion of calcitonin gene related-peptide (CGRP) and substance P (SP) in the spinal cord. However, the pathophysiological mechanisms for depletion of CGRP and SP following the neurorathic injury are still unknown. This study was performed to see whether the distribution of immunoreactivity for CGRP and SP in the superficial dorsal horn and dorsal root ganglia(DRG) was related to the distance between the DRG and injury site. To this aim, we compared two groups of rats; one group was subjected to unilateral inferior and superior caudal trunk transections at the level between the S3 and S4 spinal nerves (S34 group) and the other group at the levels between the S1 and S2, between S2 and S3 and between S3 and S4 spinal nerve (S123 group). The transections in both groups equally eliminated the inputs from the tail to the S1-3 DRG, but the distance from the S1/S2 DRG to the injury site was different between the two groups. Immunostaining with SP and CGRP antibody was done in the S1-S3 spinal cord and DRG of the two groups 1 and 12 weeks after the injury. The results obtained are as follows: 1. The immunoreactivity for CGRP and SP in the ipsilateral superficial dorsal horn and DRG decreased 1 and 12 weeks after neuropathic nerve injury. 2. The immunoreactive area of SP and CGRP in the S1 dorsal horn was smaller in the S123 group than in the S34 group, whereas that in the S3 dorsal horn was not significantly different between the two groups. The number of SP-immunoreactive DRG cells decreased on the neuropathic side as compared to the sham group's in all DRGs of experimental groups except the S1 DRG of the S34 group. These results suggest that the amounts of SP and CGRP in the dorsal horn and DRG following neuropathic injury inversely decrease according to the distance between the DRG and injury site.

용착금속부 재열균열에 대한 신뢰성 확보대책

김희진,유회수,Kim, Hee-Jin,Ryoo, Hoi-Soo 대한용접접합학회 2007 대한용접·접합학회지 Vol.25 No.2