Gene Expression Profiling in Osteoclast Precursors by Insulin Using Microarray Analysis

김홍성,이나경 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.11

The balance between bone formation by osteoblasts and destruction of mineralized bone matrix by osteoclasts is important for bone homeostasis. The increase of osteoclast differentiation by RANKL induces bone diseases such as osteoporosis. Recent studies have shown that insulin is one of main factors mediating the cross-talk between bone remodeling and energy metabolism. However, the systemic examination of insulin-induced differential gene expression profiles in osteoclasts has not been extensively studied. Here, we investigated the global effects of insulin on osteoclast precursors at the level of gene transcription by microarray analysis. The number of genes that were up-regulated by  1.5 fold after insulin treatment for 6 h, 12 h, or 24 h was 76, 73, and 39; and 96, 83, and 54 genes were down-regulated, respectively. The genes were classified by 20 biological processes or 24 molecular functions and the number of genes involved in ‘development processes’ and ‘cell proliferation and differentiation’ was 25 and 18, respectively, including Inhba, Socs, Plk3, Tnfsf4, and Plk1. The microarray results of these genes were verified by real-time RT-PCR analysis. We also compared the effects of insulin and RANKL on the expression of these genes. Most genes had a very similar pattern of expressions in insulin- and RANKL-treated cells. Interestingly, Tnfsf4 and Inhba genes were affected by insulin but not by RANKL. Taken together, these results suggest a poten-tial role for insulin in osteoclast biology, thus contributing to the understanding of the pathogenesis and development of therapeutics for numerous bone and metabolic diseases.

Poly(vinylpyrrolidone-g-acrylonitrile) 블렌드의 자외선 흡수특성에 관한 연구

김홍성,이신희 한국섬유공학회 1998 한국섬유공학회지 Vol.35 No.3

Ultraviolet radiation (UV) absorption- ability of poly (vinylpyrrolidone-g-acrylonitrile) blend and the effect of adsorption of UV-absorbers into the polymeric substrate were discussed in chemical interaction between UV-absorbers and poly (vinylpyrrolidone-g-acrylonitrile) blend as a substrate. The blend has chemical groups to interact with UV absorbers and has high visible transparency and radiation-durability. The results showed that the chemical and molecular structural character of UV absorber and of polymeric substrate affects sensitively the absorptionability of UV radiation and the adsorption-ability of UV-absorber into its substrate materials. The interaction system of 2- or 4-hydroxy in benzenoid with carbonyl in phenone or pyrrolidone was mainly investigated by UV spectrometer and FT-IR spectrometer. The blend prepared under proper constituent and condition shows excellent UV-shielding property.

Ni - silicide 형성시 기지가 되는 Si 구조변화에 따른 silicide 형성 mechanism 과 morphology 의 변화

김홍성,이재영 대한금속재료학회 1995 대한금속재료학회 학술대회 개요집 Vol.1995 No.1

폴리아크릴로니트릴과 폴리(비닐피롤리돈-그래프트-아크릴로니트릴)블렌드의 물성

김홍성,박지은 한국섬유공학회 1994 한국섬유공학회지 Vol.31 No.5

To improve the physical properties of polyacrylonitrile(PAU), the blends of PAH and poly(vinylpyrrolidone-g-acrylonitrile) (PGA) were prepared and were investigated by IR. X-ray difrractometer. Rheovibron, and Instron. PAH homopolymer and poly (vinylpyrrolidone-g- acrylonitrile) (PGA) were synthesized by suspension polymerization. These two constituents of the reactor blends were simultaneously obtained by incorporation of acrylonitrile into a little PVP backbone. Some interaction between PAN and PCA copolymer was identified, which was regarded as a dipolar interaction between the nitrile groups and the pyrrolidone carbonyl groups. All PGA were located in amorphous region of the blends. The phase structure of the blends was found to have correlation with contained quantity of PGA. The blend containing below 2 mol% of PVP had a uniform amorphous phase in which PGA was well dispersed in PAH matrix. Therefore tensile strength and elongation were increased. However, in the blend containing 10 mol% of PVP. a separated domain of PGA forms in the amorphous phase.

제어자발화 가솔린기관의 성능 특성

김홍성 한국기계가공학회 2005 한국기계가공학회지 Vol.4 No.1

In this study, A controlled auto-ignition (CAI) single cylinder gasoline engine is considered, focusing on the extension of operating conditions. The fuel is injected indirectly into electrically heated inlet air flow. Investigated are the engine performance characteristics under the wide range of operating conditions such as 32 to 63 in the air-fuel ratio, 1000 to 1800 rpm in the engine speed, and 150 to 180 in the inlet-air temperature. A controlled auto-ignition gasoline engine which has the super ultra lean-burn with self-ignition of gasoline fuel can be achieved by heating inlet air.

Anti-tumor Effect of 4-1BBL Modified Tumor Cells as Preventive and Therapeutic Vaccine

김홍성 대한의생명과학회 2022 Biomedical Science Letters Vol.28 No.4

We have previously reported that genetically modified tumor cells with 4-1BBL have anti-cancer effects in a CT26 mouse colorectal tumor model. In this study, genetically modified tumor cells with 4-1BBL were evaluated for their potential as candidates for preventive and therapeutic cancer vaccine. To identify the effect of preventive and therapeutic vaccine of genetically modified tumor cells with 4-1BBL, tumor growth pattern of CT26-4-1BBL as a cancer vaccine was examined compared to CT26-beta-gal. In therapeutic vaccination, CT26-WT was inoculated into mice and then vaccinated mice with doxorubicin (Dox)-treated CT26-beta-gal and CT26-4-1BBL (single or three times). Triple vaccination with Dox-treated tumor cell inhibited tumor growth compared to single vaccination. Vaccination with CT26-4-1BBL showed an efficient tumor growth inhibition compared to vaccination with CT26-beta-gal. For preventive vaccination, Dox-treated CT26-beta-gal and CT26-4-1BBL was vaccinated into mice with three times and then administered mice with CT26-WT. Preventive vaccination with CT26-4-1BBL showed no tumor growth. Preventive vaccination with CT26- beta-gal also led to tumor-free mice. These results suggest that genetically modified tumor cells with 4-1BBL can be used as therapeutic or preventive cancer vaccine.

Acrylonitrile과 Methyl acrylate의 수계 Redox 공중합에 있어서 Polyvinylpyrrolidone의 영향

김홍성,이신희 한국섬유공학회 1996 한국섬유공학회지 Vol.33 No.3

The kinetics of copolymerization of acrylonitrile with methyl acrylate initiated by aqueous sulite-chlorate redox system were studied in the presence of polyvinylpyrrolidone (PVP) as an emulsifier. The PVP was found to affect the polymerization rate, conversion, and relative molecular mass of polyacrylonitrile copolymer. The PVP in the polymerization solution was found to increase the polymerization rate particularly by reducing radical induction periods and the polymerization activation energy. A suitable kinetic scheme has been proposed and discussed to account for the experimental data.

액체암모니아에 의한 면 셀룰로오스 I과 II의 결정구조의 변화

김홍성,배소영 한국섬유공학회 1995 한국섬유공학회지 Vol.32 No.2

The crystal transformation, degree of crystallinity, and crystallite size of cotton cellulose, treated with liquid ammonia and dried by evaporation, wert investigated. The quantification of crystalline content was carried out by resolution of X-ray scattering intensity distribution on the assumption that all digracted intensities take the form of symmetrical Gauss distribution centering at its Bragg angle. The treatment of liquid ammonia transformed part of cellulose I and II crystals into III crystals, thereby making it a polymorphic structure. The m crystals transformed from I and II crystals, IIIa, and IIIb, had a very close lattice type mutually, but showed a considerable difference on the transformational extent, crystalline disruption behavior, and reversional ability to original crustal.

퍼지-신경망 기법을 이용한 세탁기 ( WASHER USING FUZZY LOGIC & NEURAL NETWORKS )

김홍성,한관구,이옥기,김판돌 대한전자공학회 1991 대한전자공학회 학술대회 Vol.1991 No.1

자재관리 시스템 설계 및 구현

김홍성,오상봉 대전대학교 산업기술연구소 2001 산업기술연구소 論文集 Vol.12 No.1

We developed a material management information system for Younghwa Company, which is one of small and medium-sized company producing chairs for hair dressing saloons and hospital. Younghwa Company uses more than 1,000 materials to produce more than 20 product models, In this study, we analysed the ordering process and scheduling process in the Younghwa Company and developed the information system to automate the above two processes, The material management information system consists of five subsystems, client management subsystem, product management subsystem, material management subsystem, analysis subsystem, and reprort subsystem. We also implemented the company database using Access 2000.