TRAF6 Distinctly Regulates Hematopoietic Stem and Progenitors at Different Periods of Development in Mice

김혜강,이승원,이승우 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.8

Tumor necrosis factor receptor-associated factor 6 (TRAF6) is identified as a signaling adaptor protein that regulates bone metabolism, immunity, and the development of several tissues. Therefore, its functions are closely associated with multiple diseases. TRAF6 is also involved in the regulation of hematopoiesis under steady-state conditions, but the role of TRAF6 in modulating hematopoietic stem and progenitor cells (HSPCs) during the developmental stages remains unknown. Here, we report that the deletion of TRAF6 in hematopoietic lineage cells resulted in the upregulation of HSPCs in the fetal liver at the prenatal period. However, in the early postnatal period, deletion of TRAF6 drastically diminished HSPCs in the bone marrow (BM), with severe defects in BM development and extramedullary hematopoiesis in the spleen being identified. In the analysis of adult HSPCs in a BM reconstitution setting, TRAF6 played no significant role in HSPC homeostasis, albeit it affected the development of T cells. Taken together, our results suggest that the role of TRAF6 in regulating HSPCs is altered in a spatial and temporal manner during the developmental course of mice.

급성췌장염 및 급성위염 환자의 위액내 Amylase, Lipase, pH 및 Calcium 치 변동

김혜강,박영규,이승호,김대곤,안득수 의과학연구소 1988 全北醫大論文集 Vol.12 No.3

급성 췌장염 및 급성 위염의 특이적 검사소견을 보완하기 위하여 1985년 9월부터 1987년 8월까지 약 2년간 전북의대 부속병원 내과에 입원한 환자중 임상적 및 진단적 검사상 확진된 급성 췌장염 10예와 위염 환자 7예, 건강대조군 5예를 대상으로 하여 혈청 및 위액에서 췌장효소인 amylase, lipase 그리고 pH, calcium치를 측정 검토하였다. 특히 급성 췌장염은 조기예후 인자에 의해 경증 췌장염과 중증 췌장염에서 각각 위액 amylase치는 64279.3±143511.2IU/L, 147070,4±32832.7IU/L의 유의한 증가를 보였으며 위액 lipase치 역시 각각 324.5±60.5IU/L, 141.8±36.4IU/L pH 변화는 5.8±1.6, 5.1±0.7, calcium치는 6.3±0.7mg/dl, 4.8±0.6mg/dl로 모두 유의한 증가를 보였다.급성 위염에서는 위액 amylase치는 1525.3±270.2IU/L로 유의한 증가를 보였고, 위액 lipase치 역시 calcium치 변화는 유의한 차가 없었다. 따라서 급성 췌장염에서 위액amylase, lipase, pH, calcium치 측정은 예후판정 및 보조적 진단검사의 하나로 유용하게 사용될 것으로 사료되며 급성 위염에서는 위액 내 amylase, lipase치 변동의 유의성과 병인과의 관계에서 더욱 많은 연구가 요할 것으로 본다.

소화기 종양에 대한 내시경적 완화치료

김혜강,정대영,Hye Kang Kim,Dae Young Cheung 대한소화기암연구학회 2014 Journal of digestive cancer reports Vol.2 No.1

Palliative care for cancer aims to relieve the discomfort and pain from the cancer itself and associated conditions. Gastrointestinal cancers originate from the tube like structure of gastrointestinal tract and cause complications such as obstruction, bleeding, adhesion, invasion, and perforation to adjacent organ. Recent advances in interventional endoscopy enables endoscopy physicians to do safe and effective care for gastrointestinal cancer patients. Endoscopic palliation includes stent, hemostasis, nutritional support and targeted drug delivery. Self expandable metallic stent is one of the most important modalities in gastrointestinal palliation. Through the endoscopy or over the wire pre-placed by endoscopy, stents restore the gastrointestinal luminal patency and relieve the obstructive condition. Endoscopic hemostasis is another important palliation in gastrointestinal cancer patients. Epinephrine injection, argon plasma coagulation and thermal cauterization are usual modalities for hemostasis. Histoacryl glue and fibrin glue are also available. Hemostatic nanopowder spray is newly reported effective in benign disease and is supposed to be effective also in cancer bleeding. Enteral feeding tubes including gastro- or jejunostomy and nosoduodenal tubes are placed by using endoscopic guidance. Enteral feeding tubes role as the route of easily absorbable or semi-digested nutrients and effectively maintain both patients calorie requirements and gut microenvironment. Photodynamic therapy is the one of the outstanding medical employments of photo-physics. Especially for superficial cancers in esophagus, photodynamic therapy is very useful in cancer removal and maintaining organ structure. In biliary neoplasm, photodynamic therapy is well known to be effective in cancer ablation and biliary ductal patency restoration. Targeted drug delivery is the lastest issue in palliative endoscopy. Debates and questions are still on the table. In this article, the role of endoscopic interventions in palliative care for the gastrointestinal tumors will be thoroughly reviewed.

디지털 정부 정책 텍스트 분석을 통한 기술 트렌드의 동향 파악 및 전망

김혜강,이기헌 대한산업공학회 2023 대한산업공학회 춘계학술대회논문집 Vol.2023 No.5

디지털 정부 정책 텍스트 분석을 통한 기술 트렌드의 동향 파악 및 전망

김혜강,이기헌 한국경영과학회 2023 한국경영과학회 학술대회논문집 Vol.2023 No.5

S-51 : Carcinoid tumors of gastrointestinal tract: Outcomes according to treatment modality

김혜강,전준한,조형준,이성진,김현진,정대영,박수헌,김재광,김진일 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Background: With the advance of gastrointestinal diagnostic modalities, epidemiology and clinical course of gastrointestinal neuroendocrine tumors (GI-NETs) have been changed from that of previous century. We aimed to define the characteristic of gastrointestinal carcinoid tumors in our institute for recent 5 years and compared the efficacy and safety of endoscopic treatment to operation. Methods: By analysing medical records in single tertiary academic hospital between 2007 and 2011, 91 lesions of GI-NETs were found. 66 patients with rectal carcinoid tumors underwent endoscopic mucosal resection (EMR, n=29), endoscopic submucosal dissection (EMR, n=23) or transanal endoscopic microsurgery (TEM, n=14). Results: NETs was most commonly found at rectum (76.9%), followed by stomach and duodenum (20.7%). The NETs of stomach and duodenum showed higher patient age and larger tumor size (stomach: 70.1±8.8 years, 14.5±8.2 mm; duodenum: 62.5±14.0 years, 10.3±5.4 mm) than those of rectum (49.2±11.4 years, 6.7±2.4 mm; p<0.01). For rectal NETs, local resection was performed in 66 out of 70 lesions. Histologically complete resection rate was significantly higher in ESD, TEM group (82.6%, 100%) than EMR group (65.5%). The complication rate was significantly higher in ESD, EMR group (47.8%, 18.5%) than TEM group (0%; p=0.003). However, most of complications were minor bleeding that could be managed immediately. TEM had disadvantage of significantly longer running time (TEM: 40.7±14.2 min, ESD: 18.0±13.2 min; p<0.01) and hospitalization (TEM: 5.3±1.1 day, ESD: 3.8±0.8 day; p<0.01), and also had a potential risk of anesthesia. No recurrence has been observed in all of 3 groups during a median follow-up of 24.1 months. Conclusions: The clinical course of GI-NETs depends on the respectability of primary lesion. Local resection for small GI-NETs results in comparable outcomes among the modalities. Although TEM had shown higher efficacy, endoscopic treatment seems to be more convenient than TEM for small rectal carcinoid tumors. ESD showed superior efficacy to EMR. ESD seems acceptable for small rectal and gastric carcinoid tumors.

증례 : 순환기 ; 우관상동맥에서 기원하는 영양혈관을 갖는 거대 좌심방 점액종 1예

김혜강 ( Hye Kang Kim ),이동현 ( Dong Hyeon Lee ),권태근 ( Tae Geun Gweon ),송미애 ( Mi Ae Song ),백명기 ( Myong Ki Baeg ),김정호 ( Jeong Ho Kim ),전희경 ( Hui Kyung Jeon ) 대한내과학회 2010 대한내과학회지 Vol.78 No.1

심방 점액종은 심방 중격에서 발생하여 좌심방 혹은 우심 방으로 천천히 점진적으로 커지며, 대부분은 좌회선 관상동맥에서 혈관 공급이 이루어지나 우관상동맥에서의 혈관공급은 드문 일차성 양성 종양이다. 본 증례에서 저자들은 경·중등도의 호흡곤란을 주소로 내원한 여자 환자에서 관상동맥조영술을 통해 진단된 우관상동맥에서 기시한 거대 심장 종양의 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. A 55-year-old woman was referred to our hospital with a 6-month history of dyspnea (NYHA II-III). The physical examination revealed a grade 2/6 "tumor plop," i.e., a low-pitched sound heard during early or mid-diastole. The chest X-ray showed mild cardiomegaly with lung congestion in both lower lung fields. Two-dimensional echocardiogram showed a large myocardial mass, prolapsing into the left ventricle during diastole. Chest computed tomography showed a multi-lobulated mass (6.8×4.1 cm) attached to the interatrial septum during systole and prolapsing into the left ventricle during diastole. Coronary angiography demonstrated large tumor vessels arising from and surrounding the posterior lateral branch of the right coronary artery. The mass was excised and the patient recovered uneventfully. (Korean J Med 78:104-108, 2010)

한국 군인에서 중증 아데노바이러스 폐렴의 임상 양상

허중연,김혜강,차용준,이지은,최강원 대한감염학회 2012 Infection and Chemotherapy Vol.44 No.5

Background: Adenoviral diseases generally present as self-limiting upper respiratory illnesses. However, in some cases, adenovirus can cause fatal outcomes, even in healthy adults. Severe pneumonia caused by adenovirus in young soldiers at basic military training sites have been reported. Materials and Methods: We conducted a retrospective review of the medical records of patients with pneumonia who were hospitalized in the Intensive Care Unit of the Armed Forces Capital Hospital between January 2011 and March 2012. Results: Nine patients with pneumonia who required ventilator treatment were analyzed. Adenovirus was proved to be the causative agent of the illness in six out of nine patients, three of whom died due to severe adenoviral pneumonia or adenovirus-related illness. Among these six patients, three were military trainees. Clinical features were characterized by fever (100%) and cough (100%). Other important findings included thrombocytopenia (83.3%), leukopenia (66.6%), and radiologically lobar consolidation (66.7%). Conclusions: Adenovirus appeared to be the most important pathogen of young Korean soldiers, particularly among military trainees with severe pneumonia requiring mechanical ventilation. Outbreak surveillance for adenovirus associated illness is necessary

Evaluating Antitumor Activity of Kiatomab by Targeting Cancer Stem Cell-Specific KIAA1114 Antigen in Mice

김새원,박한욱,김혜강,이승원,최소영,박윤지,이승우 대한면역학회 2019 Immune Network Vol.19 No.6

A full-length translational product of the trophinin gene, KIAA1114, is a distinctive marker of cancer stem cells in human hepatocellular carcinoma, and a mAb, Kiatomab, is specific to KIAA1114 antigen. In this study, we addressed the therapeutic potential of Kiatomab for treating both metastatic and solid tumors in mouse models. Kiatomab recognizes the linear epitope of KIAA1114, which is expressed on cell surfaces of various murine cancer cell lines. Kiatomab treatment induced potent antitumor responses in pulmonary metastasis models. Antitumor activity was mediated by the fragment crystallizable portion of Kiatomab and dependent on the host immune system. The use of Kiatomab alone as an antitumor therapy was ineffective in solid tumor models. However, in combination with cyclophosphamide, or by switching the isotype of the mAb, improved antitumor effects of Kiatomab were observed. These results suggest that Kiatomab can be used as a novel mAb for cancer immunotherapy.

국군수도병원에 입원한 지역사회 획득 폐렴 환자에서 바이러스 감염의 빈도 및 임상양상

이지은 ( Ji Eun Lee ),김혜강 ( Hye Gang Kim ),심성훈 ( Sung Hoon Shim ),차용준 ( Yong Joon Cha ),심영수 ( Young-soo Shim ) 국군의무사령부 2013 대한군진의학학술지 Vol.44 No.1

Background: Community-acquired pneumonia(CAP) is a common disease in military and can be a cause of death in previously young and healthy adults. We aimed to investigate the prevalence of viral infection in CAP and clinical characteristics of the patients hospitalized with CAP in military referral hospital. Methods: We reviewed all patients with pneumonia who admitted to the Armed Forces Capital Hospital between March. 2011 and February. 2012. Among these patients, patients who were tested for multiplex polymerase chain reaction (multiplex-PCR) for respiratory viruses with throat swab were enrolled for analysis. Results: In total, 54 patients with pneumonia were enrolled. Nineteen (35%) of patients had respiratory virus infection. The most common organisms were adenovirus(n=14, 73.7%), rhinovirus(n=5, 26.3%), and influenza A(n=2, 10.5%). Two patients with adenovirus infection died during study period. Patients with respiratory virus infection complained of rhinorrhea more commonly (47.4% vs. 14.3%; p=0.01), had lower CRP(17.39[6.02-31.1]mg/dl vs. 7.45[7.42-7.47]mg/dl, p=0.05). There were no significant differences in radiologic findings between two groups. Conclusion: Infections with respiratory viruses are common in patients who are hoapitalized with CAP, comprising 35% of all enrolled patients in our study. Our study suggests that routine testing for common respiratory viruses may be warranted for patients hoapitalized with CAP.