오미자(五味子) 헥산 분획 추출물이 고지방 식이에 의한 고지혈증 생쥐의 지질대사 및 간 조직 유전자 발현에 미치는 영향

김현영,박선미,김영균,Kim, Hyun-Young,Park, Sun-Mi,Kim, Young-Kyun 대한중풍순환신경학회 2014 대한중풍.순환신경학회지 Vol.15 No.1

■ Objectives The berries of Schisandra chinensis (Schisandra Fructus) are given the name Omiza in Koreane(五味子), and have been used asremedies for many ailments: to resist infections, increase skin health, and combat insomnia, coughing, and thirst. This study was designed to investigate the effects of Schisandra Fructus hexane fraction (SFH) on serum lipid levels in Hyperlipidemic mice. ■ Methods In this experiment, effects on total cholesterol, HDL-cholesterol, triglyceride, AST, ALT, fasting blood glucose in serum were measured. And in addition, histopathological and gene expression changes in liver tissue was also observed. ■ Results SFH did not affects weight gain, serum AST and ALT in hyperlipidemic mice. Oral administration of SFH lowered levels of total cholesterol and triglyceride, which were elevated by induction of hyperlipidemia. Finally, administration of SFH lowered fasting blood glucose significantly. And SFH also ameliorates anti-oxidative stress systems in internal organs which play key role in disease prevention. ■ Conclusion Results in our study suggest that SFH can prevent obese through regulation of dyslipidemia and hyperglycaemia.

발아 벼 부위별 추출물의 항산화활성과 Angiotensin Converting Enzyme I 저해활성

김현영,황인국,김태명,박동식,김재현,김대중,이준수,정헌상,Kim, Hyun-Young,Hwang, In-Guk,Kim, Tae-Myoung,Park, Dong-Sik,Kim, Jae-Hyun,Kim, Dae-Joong,Lee, Jun-Soo,Jeong, Heon-Sang 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.6

한국산 벼의 발아 전과 후의 부위별 및 추출용매별 항산화성분 및 활성변화를 살펴보았다. 발아 전과 후의 벼를 왕겨, 현미 및 싹으로 분리하고 각각 열수 및 70% 에탄올을 이용하여 추출하였다. 총 폴리페놀 함량은 현미 물추출물에서 발아 전 1.67에서 발아 후 5.84 mg/g으로 증가하였으며, DPPH 라디칼소거능은 벼 에탄올추출물에서 발아 전 22.95 %에서 발아 후 31.32%로 증가하였지만 왕겨와 현미 부분에서는 발아 후 감소하였다. 총 항산화력은 에탄올추출물에서 발아 전과 후에 큰 차이를 보이지 않았으나 싹 부분에서는 4.41 mg AA eq/g으로 높게 나타났다. 환원력은 에탄올 추출물 현미에서 발아 전 0.32에서 발아 후 0.45로 증가하였다. ACE 저해활성은 왕겨에서 가장 높았으며 발아 후 증가하였다. 본 연구결과 발아 시 항산화활성이 증가하는 원인에 대한 추후 연구가 필요하다 판단된다. We investigated the changes in antioxidant and angiotensin converting enzyme I (ACE) inhibitory activity in different parts of 'Ilpum' (Oryza sativa L.) rough rice before and after germination. Rough rice, either before or after germination, were separated into hull, brown rice, or sprout, and then extracted with distilled water and 70% ethanol. After germination, the total polyphenol contents of the distilled water extract of the brown rice was higher than before germination (5.84 and 1.67 mg/g, respectively). The DPPH radical scavenging activity on the unseparated rough rice ethanol extract increased from 22.95% before germination to 31.32% after germination, but it decreased in both the hull and brown rice extracts. The ABTS radical scavenging activity was highest in the sprout extract with a value of 4.41 mg AA eq/g. The reducing power of the brown rice ethanol extract increased from 0.32 before germination to 0.45 after germination. The ACE inhibitory activity of the in hull ethanol extract increased after germination. These results indicate that antioxidant capacity increases in the hull and sprout after germination could be considered having significant health benefits.

신생아 담관 낭종의 수술 필요성에 대한 고찰

김현영,이혜승,이성철,정성은,박귀원,김우기,Kim, Hyun-Young,Lee, Hye-Seung,Lee, Seong-Cheol,Jung, Sung-Eun,Park, Kwi-Won,Kim, Woo-Ki 대한소아외과학회 2005 소아외과 Vol.11 No.1

Choledochal cysts have been associated with complications such as cholangitis, pancreatitis, and malignancy of the biliary tract. Recently, the incidence of choledochal cyst in neonate and young infant is increasing due to advances in diagnostic imaging. The aim of this study is to investigate the rationale of excision of choledochal cyst during the neonatal period. The clinical outcome and correlation between age at surgery and the degree of liver fibrosis were reviewed retrospectively. A total of 198 patients with choledochal cyst who were managed surgically between January 1985 and December 2000 at the Department of Surgery, Seoul National University Children's Hospital were included in this study. The overall outcome and the outcome of patients who were managed surgically during the neonatal period were compared. Correlation between age and the degree of liver fibrosis was evaluated by chi-square test and Pearson exact test. The mean age of the patients was 2 years 7 months (ranged from 5 days to 15 years). Mean postoperative follow-up period was 7 years 1 month (7 months to 20 years). The results are as follows. Twelve patients (6%) had postoperative complications, cholangitis (7), bleeding (4) and ileus (1). Eleven patients operated during the neonatal period had no postoperative complications. The positive correlation between age group and degree of liver fibrosis was statistically significant (chi-square: p=0.0165, Pearson exact test: p=0.019). The results support the rationale that excision of choledochal cyst can be performed safely without increasing morbidity in neonates.

F344 랫드를 이용한 이황화메틸의 아급성 흡입독성연구

김현영,이성배,한정희,정용현,김형진,신진영,신동호,김종춘,이용묵,Kim Hyeon-Yeong,Lee Sung-Bae,Han Jung-Hee,Chung Yong-Hyun,Kim Hyoung-Chin,Shin Jin-Young,Shin Dong-Ho,Kim Jong-Choon,Lee Young-Mook 한국생명과학회 2005 생명과학회지 Vol.15 No.1

이황화메틸의 반복 흡입노출에 의한 아급성 독성 잠재력을 평가하기 위해 암수 랫드에게 0, 5, 25 및 125 ppm용량으로 21일간 반복 흡입노출하고, 일반증상과 체중, 사료섭취 량, 혈액치, 혈청생화학치 및 부검소견을 관찰하였다. 시험결과, 랫드에게 이황화메틸을 3주간 반복 흡입노출하면 125 ppm의 농도에서 체중증가의 억제와 사료섭취 량의 감소를 유발하나, 혈액 및 혈청생화학치에는 어떠한 이상도 유발하지 않는 것으로 나타났다. 본 시험 조건 하에서 이황화메틸의 표적 장기는 관찰되지 않았으며, 무해용량은 암수 모두 25 ppm으로 사료된다. The purpose of this study was to investigate the potential subacute toxicity of dimethyl disulfide by 3 weeks inhalation in F344 rats. The test article, dimethyl disulfide was exposed by inhalation to male and female rats at dose levels of 0, 5, 25, or 125 ppm/6 hrs/ day for 3 weeks. Five rats/ sex/ group were sacrificed on day 4 after the initiation of treatment, while 5 rats/ sex/ group were sacrificed at the end of treatment period. During the test period, clinical signs, mortality, body weights, food consumption, hematology, serum biochemistry, and gross findings were examined. Slight decreases in body weight gain were noted in both sexes of the highest dose group in a dose dependent manner but were only statistically different from the control animals in males of the group. A slight non-significant reduction in food consumption were also noted in the both sexes of the highest dose group. There were no adverse effects on mortality, clinical signs, hematology, serum biochemistry, and necropsy findings at any dose tested. Based on these results, it was concluded that the 3 weeks repeated dose of dimethyl disulfide by inhalation resulted in suppressed body weight gain and decreased food consumption at 125 ppm of both sexes. In the present experimental conditions, the target organ was not determined in rats. The no­observed-adverse-effect level (NOAEL) was considered to be 25 ppm/6 hrs/day for both sexes.

영산강 하구둑 홍수관리시스템

김현영,박승우,박창언,Kim Hyun-young,Park Seung-woo,Park Chang-eon 한국관개배수위원회 1994 한국관개배수논문집 Vol.1 No.1

In order to control the flood effectively, Ministry of Construction has implemented the tele - metering(TM) facilities on the Young-san River Basin which has the area of 3,471$km^2$. Rural Development Corporation which is responsible for the es

산전 진단된 폐외분리증 2예

김현영,손동우,김석영,김지은,하승연,Kim, Hyun-Young,Son, Dong-Woo,Kim, Seok-Yong,Kim, Jee-Eun,Ha, Seung-Yeon 대한소아외과학회 2009 소아외과 Vol.15 No.2

Congenital thoracic malformations such as intra- and extra-pulmonary sequestration, cystic adenomatoid malformation, congenital pulmonary airway malformation, malinosculation, bronchogenic cyst, reduplication cyst, and foregut cyst are frequently detected on routine prenatal ultrasound. There are some controversies about treatment for postnatally persistent pulmonary sequestration. Some authors recommend expectant long term follow up but most authors advocate elective surgical excision because of complication such as respiratory distress, infection, intrathoracic bleeding, haemoptysis, cardiac failure, and potential risk of malignancy. We experienced 2 cases of prenatally diagnosed extrapulmonary sequestration which were located in the subdiaphragmatic retroperitoneum. Resections were performed at 2 months and 4 months of age using intraabdominal approach. There were no complications. In conclusion, if the prenatally diagnosed extrapulmonary sequestration remained postnatally, early operation might reduce morbidity related to extrapulmonary sequestration and parental anxiety without any postoperative complication.

새싹 밀의 추출용매에 따른 생리활성 평가

김현영,서혜영,서우덕,이미자,함현미,Kim, Hyun Young,Seo, Hye-Young,Seo, Woo Duck,Lee, Mi Ja,Ham, Hyeonmi 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.6

The purpose of this study was to examine biological activities, including total contents of polyphenol, antioxidant activities, inhibitory activities of tyrosinase, and protective effect against oxidative stress in the HepG2 cells of ethanol extracts from wheat sprout. The antioxidant activity of extracts was determined by ABTS and DPPH radical scavenging activities. Ethanol extracts were tested using different ethanol concentrations (0%, 30%, 50%, 80% and 95%, respectively). The highest amount of total polyphenol was extracted by 50% and 80% ethanol which was 26.3 and 26.8 mg gallic acid equivalents/g sample, respectively. High levels of ABTS and DPPH radical scavenging activity were found in 50% ethanol (26.7 and 15.0 mg TEAC/g sample, respectively) and 80% (24.3 and 16.1 mg TEAC/g sample, respectively) ethanol extracts. Also, 50% and 80% ethanol extracts indicated higher inhibitory activities of tyrosinase compared with other extracts. In the cell-based assay, pre-treatment of the HepG2 cells with wheat sprout extracts prevented the cell damage induced by TBHP (tert-butyl hydroperoxide). The results of this study indicate that wheat sprout has significantly higher diverse biological activities and apparently has significant health benefits.

Mouse의 신장상피세포에서 패장(敗醬)추출물이 산화 스트레스 및 NF-${\kappa}B$ signaling에 미치는 영향

김현영,장수영,최규호,신현철,Kim, Hyun-Young,Jang, Soo-Young,Choi, Gyu-Ho,Shin, Hyeon-Cheol 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.1

Objectives : The aims of this study were to investigate the cytoprotective, antioxidative and inflammation genes inhibitory effects of Patriniae Radix on the mouse LLC-$PK_1$ cells (renal epithelial cells). Methods : The cytoprotective effect of Patriniae Radix was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of superoxide anion radical (${\cdot}{O_2}^-$) by 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), nitric oxide (NO) by 4,5-diaminofluorescein (DAF-2), peroxynitrite ($ONOO^-$) by dihyldrorhodamine 123 (DHR 123) and prostaglandin $E_2$ ($PGE_2$) by $PGE_2$ immunoassay on $H_2O_2$-treated LLC-$PK_1$ cells. For measuring of inflammation genes inhibitory effects, western blot was performed to detect IKK-$\alpha$, phospho-$I{\kappa}B-\alpha$, NF-${\kappa}B$ (p50, p65), COX-2, iNOS, IL-$1{\beta}$ and VCAM-1 protein level in cytosol fractions from LLC-$PK_1$ cells. Results : Patriniae Radix extract reduced the $H_2O_2$-induced cell death and inhibited the amount of $H_2O_2$-induced ${\cdot}{O_2}^-$, NO, $ONOO^-$, $PGE_2$ generation dose-dependently on the mouse LLC-$PK_1$ cells in vitro. Also Patriniae Radix extract inhibited the expression of IKK-$\alpha$, phospho-$I{\kappa}B-\alpha$, COX-2, iNOS, IL-$1\beta$ and VCAM-1 genes dose-dependently by means of decreasing activation of NF-${\kappa}B$. Conclusions : According to above results, it was identified that Patriniae Radix had the cytoprotective, antioxidative and inflammation genes inhibitory effects. So it was suggested that Patriniae Radix would be effective to the treatment for the inflammatory process and inflammation-related diseases.

조절지의 용량 결정방법

김현영,Kim Hyun-Young 한국관개배수위원회 2000 한국관개배수논문집 Vol.7 No.2

유한요소해석을 통한 다공성 보의 거동 분석

김현영,김준식,Kim, Hyun-Young,Kim, Jun-Sik 한국전산구조공학회 2020 한국전산구조공학회논문집 Vol.33 No.3

In this study, various types of porous beams were designed and analyzed to examine the relationship between the behavior of a porous beam and certain nonlocal parameters. The nonlocal parameters were defined as functions of the conditions of defects in the porous material. Finite element analysis was conducted on the beams under typical boundary and loading conditions. Beams with stiffeners having the same dimensions as the defects in the porous beams were also analyzed. The deformation tendency of these beams was determined and described in terms of the nonlocal parameters. The deformation of a porous beam was linearly proportional to the square of the diameters of the defects, whereas that of a beam with a stiffener was linearly proportional to the cube of the diameter of the stiffener. Furthermore, for a stiffened beam with axial loading, the results derived from a 3D solid element and those under 2D plane stress conditions were different. 본 연구에서는 다공성 보와 논로컬 매개변수 사이의 관계에 대한 유한요소해석을 수행한다. 논로컬 매개변수는 다공성 보의 결함을 표현하는 변수들로 정의하여, 하중조건 및 경계조건에 대한 수치모사를 통해 계산한다. 다공성 보와는 반대 개념의 결함을 가지는 보에 대한 해석도 수행하였다. 이러한 보들의 거동은 논로컬 매개변수의 항으로 표현하였으며, 이 매개변수는 구멍의 지름의 제곱 그리고 원기둥 지름의 세제곱에 비례하는 것을 확인하였다. 특히 작은 원기둥을 가지는 보에 축 하중을 가하는 경우, 예상과는 다르게 3차원 유한요소 해석 결과와 2차원 평면응력 해석 결과는 다름을 알 수 있었다.