2016년 노벨 물리학상

김찬주 우리교육 2016 우리교육 Vol.266 No.-

부체형 HPV-16DNA 를 함유한 자궁경부암에서 HPV-16URR 유전자의 변이에 따른 전사 활성도 증가

김찬주,김은주,엄수종,박종섭,남궁성은,이해남,나종구,김승조 대한부인종양 콜포스코피학회 1998 Journal of Gynecologic Oncology Vol.9 No.4

HPV E2 protein is known to act as a negative regulator of transcription and the disruption of E2 open reading frame by HPV integration can release suppression of E6 and E7 mRNA expression, resulting in uncontrolled cellular growth and malignant transformation by inactivating tumor suppressor gene products (p53, pRb). YY1 mutation of HPV URR has been suggested as one of indicator that explains development of cervical neoplasia by episomal type of HPV. To extend this hypothesis, we examined whether mutation(s) in specific sites of HPV URR is functionally related to the invasiveness of cervical neoplasia and the physical status of HPV DNA. The URR sequences were obtained by PCR amplification of HPV-16 genome from CIN and invasive cancer patients, cloned into pUC18 for sequencing, and into pBLCAT8+ for functional CAT assay. Our previous data classified HPV-infected patients into three groups: 3 cancer cases carrying episomal HPV DNA; 12 cancer cases carrying integrated HPV DNA; 12 CIN cases carrying episomal HPV DNA. The specific variants in HPV-16 URR were found in Korean women: GA transition at nt 7520 (100%, 27/27), AC transition at nt 7729 (70%; 19/27), and GA transition at nt 7841 (78%; 21/27). Selective mutations were observed at the YY1-binding sites of HPV-16 URR in the 3 patients with invasive cervical cancer, who having the episomal forms of HPV-16 DNA: AC transition at nt 7484 and GA transition at nt 7488 (YY1-binding site 2; from 7481 to 7489). Additionally, CT transition at nt 7785 (YY1-binding site 3; from 7781 to 7790) was found from 2 of 3 patients. No YY1 site mutations were detected in the 12 CIN patients and in the HPV-integrated invasive cancer patients. To determine whether these mutations have effect on the expression of HPV E6/E7 genes driven by URR, the transient transfection assay was employed using URR-CAT reporter plasmid. The relative activities of three URR mutants from episomal HPV-16 DNA of cervical cancers were 2- to 4-fold higher than that of HPV-16 URR prototype. In contrast, the URRs from integrated HPV-16 DNA in cervical cancer and from episomal HPV-16 DNA in CIN, where no mutation of the YY1-binding site was detected, showed similar levels of promoter activity to that of URR prototype. Our results support the hypothesis that the mutation at YY1 binding site is functionally related to the development of cervical neoplasia caused by episomal HPV-16 DNA in Korean cervical cancer patient. Thus, mutation in YY1 site of episomal HPV-16 URR may play a role of HPV integration in the progression of cervical cancer.

산지생산자조직의 현황과 육성전략

김찬주 한국식품유통학회 2020 한국식품유통학회 학술대회 Vol.- No.하계

자궁 경부암

김찬주 약업신문사 1999 의약정보 Vol.1999 No.10

포스타 : 부인종양학 ; 자궁경부암 세포의 성장에 미치는 에스트로겐의 효과

김찬주,엄수종,박종섭,김태연,이준모,김승조,남궁성은 대한산부인과학회 1998 대한산부인과학회 학술대회 Vol.82 No.-

의복상품특성에 따른 소비자 위험지각 및 위험감소행동에 관한 연구

김찬주 한국복식학회 1995 服飾 Vol.25 No.-

This research was intended to investigate how risk perception and risk reduction behavior by consumers differ according to different product characteristics of clothing. the reponses of 318 female college students living in Seoul and surrounding vicinities were collected an analyzed. Inner wear, blue-jean pants, coat were selected as representing each clothing product characteristics. Frequencies distribution, regression and correlation coefficient were utilized ofr statistical analysis Results are as follows. 1. The type of perceived risk and risk reduction behavior differed according to product characteristics of clothing. Physical and performance risk were more highly perceived for the purchase of innerwear. However, for the purchase of jean pants and coat, socio-psycho-logical and economic risk were also perceived highly becasue the rate of fashion change, social symbolism, and coordination with other clothing items become more important characteristics. To reduce perceived risk, dependency on past purchase experiences and shopping were mostly preferred method regardless of product characteristics of clothing. 2. Risk type as determinant variables for predicting overall risk differed according to product characteristics of clothing. But fashionability and usefulness were common determinant risk variables, which identifies typical characteristics of clothing product.

자궁경부암 동물 모델에서 PEG/PLLA copolymer sysetm을 사용한 Paclitaxel 국소 투여의 효과

김찬주,이용석,이근호,정병문,김태우,강태흥,김희숙,박종섭 대한부인종양학회 2008 Journal of Gynecologic Oncology Vol.19 No.1

Objective : Paclitaxel is one of the most effective antineoplastic drugs. HPV-related cervical lesions have only managed with invasive procedure or observation. After the introduction of PHV prophylactic vaccine, there are no remarkable improvements. This ineffectiveness can in part be attributed to inadequate durg and delivery methods. Topical drug administration with temperature sensitive copolymer gels are useful approaches to clinical situation. In this study, we evaluated the activity of multiblock copolymers of PEG/PLA (poly(L-lactic acid)/polyethylene glycol) gels with paclitaxel (PTX) formulation administered by topical treatment to mice bearing human cervical cancer cell lines (HeLa). Methods : We have synthesized gels of PEG/PLLA (poly(L-lactic acid)/polyethylene glycol) multiblock copolymers containing Paclitaxel which have temperature-sensitivecharacteristics. This Paclitaxel- containg copolymers has the sol-gel-sol transition temperature at body temperature. The efficacy of PTX in PEG/PLA mutiblock copolymer micelle were conducted in HeLa-tumor bearing Balb/c Nu/Nu athymic mice at an equivalent paclitaxel dose of 10 mg/kg with 48 hr interval. The inhibition of tumor growth was evaluated after 8days of treatment. Tumors were harvested at day 10 and stained with hematoxylin and eosine to measure tumor. RESULTS: PTX-containing PEG/PLA mutiblock copolymer significantly decreased tumor growth at day 8, as measured by tumor size; ie, PEG/PLA mutiblock copolymer only goup ; 1.43+0.26m versus intraperitoneal treatment of Paclitaxel : 0.75+0.07mm and topical treatment of PTX-containing PEG/PLA copolymer containing Paclitaxel : 0.28mm (Min; 0.1mm-Max:0.8mm) CONCLUSION: This demonstration that PTX-containing PEG/PLA mutiblock copolymer have a useful topical drug deliversy system carrying temperature sensitive characetersitics in HPV-related cervical lesions. 목적 : Paclitaxel은 최근 널리사용되는 가장 효과적인 항암제이다. HPV관련성 자궁 경부 질환은 최근 개발된 HPV예방백신이 있지만, 침습적인 수술방법이나 관찰외에는 뚜렷한 치료법이 없다. 그 이유는 현재까지 개발된 효과적인 치료 약물과 부작용이 적은 약물 투여법이 없기 때문이다. 이에 온도 민감성 copolymer 젤을 국소적으 로 투여하는 방법이 효과적인 임상 적용에 유리하다. 이에 Paclitaxel(PTX)을 함유하고 체온 범위에서 젤이 되 는 온도 민감성 PEG/PLA (poly(L-lactic acid)/polyethylene glycol) 을 HPV양성인 자궁 경부암 세포주(HELa) 의 성장 억제에 효과가 있는지 알아보았다. 방법: Paclitaxel을 함유한 PEG/PLLA (poly(L-lactic acid)/polyethylene glycol) multiblock copolymer중 체 온 범위에서 젤이 되도록 만들어 사용하였다. Paclitaxel-PEG/PLLA 젤의 효과는 Balb/c Nu/Nu athymic mice에 대하여 주입된 HeLa 세포주의 크기 변화를 측정하였다. 48시간 간격으로 2회 Paclitaxel (10mg/kg)을 투여 한 뒤8일째까지 종양 크기를 측정하고, 마지막날 종양을 적출하여 헤마톡실린-에오신 염색으로 크기를 확인하였 다. 결과:Paclitaxel- PEG/PLA 다원 공중합체를 투여한 지 8일째에, 쥐에서 xenograft tumors (HeLa cells; HPV-18 positive state)의 종양성장 억제가 관찰이 되었다. PEG/PLA 다원 공중합체만 투여한 경우에는 종양의 크기가 1.43+0.26mm 이였으며, Paclitaxel을 복강내 주사한 경우에는 0.75+0.07mm 였다. Paclitaxel-PEG/PLA copolymer 를 경피적으로투여한 경우에 0.28m (최소 0.1mm-최대 0.8mm)였다. 결론 : HPV-관련성 자궁 경부 질환의 치료에 Paclitaxel을 함유한 PEG/PLA mutiblock copolymer는 체온 범위에 서 젤이 되는 성질을 가진 유용한 약물치료법이 될 것이다.

트윈세대의 외모와 스타일에 대한 의식

김찬주,김용주 한국의류학회 2006 한국의류학회지 Vol.30 No.6

2004학년도 수능의 새로운 오류 발굴

김찬주 우리교육 2017 우리교육 Vol.270 No.-

스마트 APC 추진현황 및 계획

김찬주 한국식품유통학회 2022 한국식품유통학회 학술대회 Vol.- No.하계