양송이 배지에서 유래한 Lipase 생산균을 이용한 바이오디젤 생산

김현희,김찬겸,한창훈,이찬중,공원식,윤민호 한국버섯학회 2015 한국버섯학회지 Vol.13 No.1

양송이 수확후 배지로부터 lipase 생산균을 분리하여 16S rDNA 유전자 분석을 통해 동정한 결과, Burkholderia cepacia ATCC와 99.8% 상동성을 나타냈다. 분리균 B. cepacia 배양여액 중에 함유된 효소단백질을 70% 황산암모늄으로 침전시켜 crude lipase를 회수하였다. 고정화 효소를 제조하기 위하여 crude lipase(CL)과 Novozyme lipase(NL)을 cross-linking 법에 의해 Silane화된 Silicagel에 고정화 시킨 결과, immobilized CL(ICL)은 61%, immobilized NL(INL)은 72%의 잔존활성을 유지하였다. 중성지방 Canola oil을 알칼리(NaOH) 촉매와 효소(CL 및 ICL) 촉매를 이용하여 지방산(fatty acid)으로 분해한 후, methanolysis에 의한 에스터전이반응(trans-esterification)을 통해 지방산으로부터 전환된 바이오디젤(fatty acid methyl ester, FAME)의 종류와 수율을 비교 하였다. 생성된 총 FAME 함량은 NaOH 781mg L-1, free lipase 681mg L-1, 고정화 lipase 598mg L-1순으로 높았으며, 지방산 조성별 FAME 함량은 linoleic acid(C18:1)가 약 50%로 가장 높았으며, stearic acid(C18:0)가 22%정도의 높은 수준이었다. 또한 반응시간이 증가함에 따라 CL과 ICL 모두 불포화지방산 FAME의 조성비는 감소하고, 상대적으로 포화지방산 FAME의 조성비는 증가하는 경향을 보여 lipase 효소가 transesterification 활성과 interesterification 활성을 동시에 가지는 것으로 여겨진다. 고정화효소의 잔여활성은 반복회수가 증가함에 따라 서서히 감소하여 4회 반복 후, 초기 활성도에 비해 ICL은 34% 와 INL은 21%까지 감소하였다. A lipase producing bacterium was isolated from button mushroom bed, which showing high clear zone on agar media containing Tributyrin as the substrate. The strain was identified as Burkholderia cepacia by analysis of 16S rDNA gene sequence. Crude lipase (CL) was partially purified from 70% ammonium sulfate precipitation using the culture filtrate of B. cepacia. Immobilized lipases were prepared by cross-linking method with CL from B. cepacia and Novozyme lipase (NL) onto silanized Silica-gel as support. Residual activitiy of the immobilized CL (ICL) and immobilized NL (INL) was maintained upto 61% and 72%, respectively. Biodiesel (Fatty acid methyl ester, FAME) was recovered by transesterification and methanolysis of Canola oil using NaOH, CL and ICL as the catalysts to compare the composition of fatty acids and the yield of FAME. Total FAME content was NaOH 781mg L-1, CL 681mg L-1 and ICL 596mg L-1, in which the highest levels of FAME was observed to 50% oleic acid (C18:1) and 22% stearic acid (C18:0). In addition, the unsaturated FAME (C18:1, C18:2) decreased, while saturated FAME (C16:0, C18:0) increased according to increasing the reaction times with both CL and ICL, supporting CL possess both transesterification and interesterification activity. When reusability of ICL and INL was estimated by using the continuous reaction of 4 cycles, the activity of ICL and INL was respectively maintained 66% and 79% until the fourth reaction.

양송이 배지에서 유래한 Lipase 생산균을 이용한 바이오디젤 생산

김현희,김찬겸,한창훈,이찬중,공원식,윤민호 한국버섯학회 2015 한국버섯학회지 Vol.13 No.1

A lipase producing bacterium was isolated from button mushroom bed, which showing high clear zone on agar media containing Tributyrin as the substrate. The strain was identified as Burkholderia cepacia by analysis of 16S rDNA gene sequence. Crude lipase (CL) was partially purified from 70% ammonium sulfate precipitation using the culture filtrate of B. cepacia. Immobilized lipases were prepared by cross-linking method with CL from B. cepacia and Novozyme lipase (NL) onto silanized Silica-gel as support. Residual activitiy of the immobilized CL (ICL) and immobilized NL (INL) was maintained upto 61% and 72%, respectively. Biodiesel (Fatty acid methyl ester, FAME) was recovered by transesterification and methanolysis of Canola oil using NaOH, CL and ICL as the catalysts to compare the composition of fatty acids and the yield of FAME. Total FAME content was NaOH 781 mg L-1, CL 681 mg L-1 and ICL 596 mg L-1, in which the highest levels of FAME was observed to 50% oleic acid (C18:1) and 22% stearic acid (C18:0). In addition, the unsaturated FAME (C18:1, C18:2) decreased, while saturated FAME (C16:0, C18:0) increased according to increasing the reaction times with both CL and ICL, supporting CL possess both transesterification and interesterification activity. When reusability of ICL and INL was estimated by using the continuous reaction of 4 cycles, the activity of ICL and INL was respectively maintained 66% and 79% until the fourth reaction.

태권도수련이 초등학생의 지적, 정서적 및 사회적 생활태도에 미치는 영향 : focusing on the northern area of Kung-Ki province

김관현,김찬겸,문원배 龍仁大學校 體育科學硏究所 1998 體育科學硏究論叢 Vol.8 No.1

The subject of this research is to offer the materials to help Tae-Kwan-Do education of children. School grade, level of Tae-Kwan-Do and year of practice were set as independent factors to investigate the effects of Tae-Kwan-Do practice to intellectual, emotional and social behavior of elementary student, and aspects of school life, emotional development, manner of living and physical development were set as dependent factors to find out how much effect does Tae-Kwan-Do had on children's social development. Questionnaires were structured with 5 point scale and sent to total 126 persons to verify the theory that school grade, level of Tae-Kwan-Do, practice year will result a difference in school life, emotional development, manner of living and physical development. The conclusion of the study form this investigation is ans followed: 1. Tae-Kwan-Do practice results the similar difference (p(.01) in emotional development according to the school grade 5-6 and grade 3-4 showed the similar difference from later analysis. 2. Each level of Tae-Kwan-Do in school life showed similar difference (p(.05) and later analysis showed that between level 3. and level 1 and lower results similar difference. Aspect of emotional development also showed similar difference that later analysis showed that between level 3. and 1 lower results similar difference. 3. The year of practice in school life showed similar difference (p(.01) and later analysis showed that between 3 year, and less than one year results similar difference. The year of practice in emotional development showed very similar difference (p(.001) and later analysis showed that between longer 3 years, 2 years and less than one year showed similar difference. Also in physical development showed very similar difference and more than 3 years and less than one year showed similar difference from later analysis.

양송이 버섯퇴비에 의한 폐광산 중금속 분해능 평가

김선중,김찬겸,노재근,공원식,윤민호 한국버섯학회 2012 버섯 Vol.16 No.2

다량의 중금속을 함유하고있는 휴, 폐광산 토양을 복원하기 위한 복토재료로서의 양송이 퇴비의 이용 가능성을 확인하기 위하여 양송이퇴비의 중금속 별 흡착능과 흡착특성을 평가하였다. Mn2+, Fe2+, Zn2+을 함유한 폐광산토양3: 양송이 퇴비2: 증류수 5의 비율로 혼합하여 컬럼에 넣고 반응시간별로 Inductively Coupled Plasma (ICP-AES)를 이용하여 용출된 중금속을 분석하였다. Mn2+의 경우 버섯퇴비를 포함한 반응조에서 미생물의 존재유무와 상관없이 대조군에 비하여 비슷하거나 높은 용출량을 나타냈으나, Fe2+은 멸균퇴비보다 미생물을 포함한 반응조에서 용출량이 월등히 높게 나타났다. 또한 Zn2+의 경우 멸균 처리된 시료의 경우 대조군과 비슷한 용출량을 나타냈지만, 미생물을 포함한 버섯퇴비로 처리 시 반응 시간이 지남에 따라 용출량이 점차 감소하였다.

서울교통공사 SPASS -Beacon을 이용한 Hi-pass Subway System

조제영,김영민,김찬겸,이용기,김진호,이준영,정원식 서비스마케팅학회 2017 서비스마케팅학회 학술대회 발표논문집 Vol.2017 No.11

Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition

이상준,최한석,김찬겸,락치미,박철환,김승욱 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-

Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenesSUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increasein 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditionssuccessfully increased the 2,3-butanediol production. In batch fermentation, 93.75 g/L of 2,3-butanediolwas obtained within 54 h, along with 0.49 g/g of yield and 1.74 g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, withproduction, yield and productivity of 126.10 g/L, 0.38 g/g and 2.10 g/L/h, respectively.

수동운전방식에서의 PSD 출입문 신호반응 시간 개선 방안 연구

이무호(Lee moo ho),김찬겸(Kim chan cum),이석종(Lee suk jong),이수영(Lee soo young) 한국철도학회 2008 한국철도학회 학술발표대회논문집 Vol.- No.-

Platform Screen Door(PSD) has been installed and operated at seoul subway line 1~4 in the manual train operation mode(ATS/ATC) by SeoulMetro since 2005. SeoulMetro uses the wireless (RF) communication system and the train door detection system for the link between the train and PSD doors opening/closing motion in the manual train operation mode. For the convenience and safety of passengers, the train doors and the PSD doors opening/closing shall be synchronized as much as possible. In ATO(Automatic Train Operation) mode which provides the interface between train control system and PSD system, ATO signaling system makes the train doors and PSD doors open/close command signals systematically, so PSD doors can be opened/closed almost simultaneously with the train doors. But, in the manual train control(ATS/ATC) mode, PSD system needs to detect the train doors open/close operation and make PSD open/close command signals to actuate PSD doors. These PSD open/close commanding process cause time delay of PSD doors opening/closing motion in response to the train doors opening/closing motion. Sometimes the response delay time can be over 1 second, which is not proper to operate PSD, and need to be reduced This paper presents the reduction method of the PSD response delay time to improve the convenience and safety of passengers.