Induction of erythroleukaemia by F - MuLV and Purification of Erythropoietin

김정곤 한국유전학회 1986 Genes & Genomics Vol.8 No.1

Friend Murine Leukemia Virus (F-MuLV) is affective against 2-day old new borns in NFS/n Swiss mice. Infected babies suffer from splenomegaly and anemia at the age of 4-5 weeks beyond which the condition worsens and is fatal. However, the spleen size can be reduced by hypertransfusion for 8 weeks. If left alone after this treatment, the spleen enlarges and becomes palpable again. This time the spleen cells are transplantable and inflict the same disease if injected to normal adults. While these leukemic cells were considered to be hormone-independent, addition of erythropoietin (EPO) was found to be essential for maximum growth in culture. Influence of EPO on the in vivo growth of leukemic cells was indicated by our findings that administration of the hormone caused a more severe leukemia and rapid death while, transfusion of red blood cells, which lowers endogenous EPO, led to increased survival of leukemic mice. It is suggested that hormone-associated therapy may be efficacious in the treatment of this and perhaps, other leukemias. Several erythroleukemic cell lines released factors which stimulated erythropoiesis in vivo and in vitro. A simple five step scheme has been devised which allows purification of this erythropoietic activity to apparent homogeneity. The methods employed included lectin affinity chromatography (wheat germ agglutinin), gel filteration (ultro gel ACA 44), ion exchange, hydroxylapatite, and high performance liquid chromatography, Following polyacrylamide gel electrophoresis, biological activity was recovered in an area corresponding to a molecular weight of 35,000 daltons. Silver staining of a polyacrylamide gel after electrophoresis of our most purified preparation revealed a single band of 35,000 daltons.

벼 건답직파재배(乾畓直播栽培)에서 절수재배(節水栽培)가 수량(收量) 및 미질(米質)에 미치는 영향(影響)

김정곤,강양순,윤영환,정남진 한국육종학회 2000 한국육종학회 학술발표회 발표요지 Vol.32 No.1

Three new records of the subfamily Mirinae (Hemiptera: Heteroptera: Miridae) from the Korean Peninsula

김정곤,정성훈 국립중앙과학관 2018 Journal of Asia-Pacific Biodiversity Vol.11 No.2

A total of three new records of the plant bug subfamily Mirinae (Hemiptera: Heteroptera: Miridae) are reported from the Korean Peninsula with findings of Apolygus fraxinicola (Kerzhner, 1988), Neolygus juglandis (Kerzhner, 1988), and Polymerus amurensis Kerzhner, 1988. Diagnoses, descriptions of genitalia, and biological information of each species are provided.

한국토양비료학회 발표요지 : 도시개발에 따른 농지전용실태에 관한 조사연구 ( 대구 및 울산지역을 중심으로 )

김정곤,정연태,예양해,박래경 한국토양비료학회 1982 한국토양비료학회 학술발표회 Vol.1982 No.28

지역단위 무역피해 지원제도의 주요국 운영사례 분석 및 시사점

김정곤,김균태 對外經濟政策硏究院 地域情報센터 2011 연구보고 Vol.2011 No.-

개방의 피해는 기업과 근로자에게만 한정되지 않는다. 무역개방으로 인한 피해는 특정 지역에 집중되는 경우가 많은바, 해당 산업 및 근로자뿐만 아니라 그 산업과 근로자에 의존한 지역경제의 구조조정을 야기하며, 고용 감소 등으로 인해 세수입이 감소되어 지방자치단체의 재정을 악화시킬 수 있다. 농어업 분야에 대한 FTA 피해 지원제도를 제외하면, 우리나라에서는 무역피해에 특정된 지역단위 지원제도가 운영되지 않고 있다. 그러나 한·EU FTA가 발효되고, 한·미 FTA 역시 비준을 앞둔 현시점에서 농업 분야 이외에 제조업, 서비스업 등 전산업을 아우르는 보편적인 무역피해 지역단위 지원제도의 도입을 검토할 시점에 이르렀다. 이 연구는 EU의 유럽구조기금(European Structural Fund) 및 캐나다의 침엽수산업과 공동체의 경제조정계획(SICEAI: Softwood Industry and Community Economic Adjustment Initiative), 그리고 미국의 공동체 구조조정 및 투자 프로그램(CAIP: Community Adjustment and Investment Program)과 2009년 도입된 공동체 TAA(Community Trade Adjustment Assistance)와 같은 해외의 지역단위 무역피해 지원제도 운영사례를 분석하고, 이를 통해 유사 제도의 필요성과 이를 도입할 경우 고려해야 할 정책적 시사점을 도출하였다. 이 연구에 따르면, 무역피해에 대한 지역단위 지원제도의 도입 시 고려해야 할 주요 사항은 다음과 같다. 즉, 지역단위 무역피해 지원제도는 1) 적절히 운영될 경우 지역경제의 새로운 발전 동력과 일자리 창출에 기여할 수 있는 유용한 수단이며, 단기적인 피해보상 중심의 무역피해 지원제도 및 여타 지역발전정책을 보완할 수 있고, 2) 장기적인 지역경제 구조개선에 기여하는 방향으로 운영되는 것이 바람직하며, 3) 지역공동체의 자발성에 기초할 때 효과적으로 운영될 수 있으며, 4) 중앙정부와 지역 간의 적절한 역할 분담 및 협력체계 구축이 중요하다. 또한 5) 운영 시 책임 부처의 단순화 또는 중앙 부처 간의 효과적인 협력체계 구축이 필요하고, 6) 투명한 제도 운영과 적절한 사후 관리가 뒷받침되어야 하며, 7) 정해진 기간 동안 시의적절하게 제공되어야 하고, 8) 현실적이고 객관적인 지원대상 선정기준의 마련이 필요하다. The impact of trade liberalization is not limited to workers, firms, farmers, and fishermen. Communities in which these groups are located also experience massive layoffs and earnings losses. In this sense, this report examines trade adjustment assistance (TAA) programs for communities of the EU, Canada and the United States in order to derive practical policy implications out of it for the Korean government. TAA for communities is likely to be a useful channel which contributes to restructuring a regional economy if it is managed in proper ways. Community-initiation, efficient cooperation between central and regional government, transparency of program management, ex-post monitoring, and time-boundedness of supports are important factors that make TAA for communities successful. Especially, TAA for communities should be designed so that it contributes to a long-term economic restructuring plan of the region.

폴 크레의 미술관건축에 나타난 보자르적 특성

김정곤 한국문화공간건축학회 2006 한국문화공간건축학회논문집 Vol.- No.15

This study tries to clarify the characteristics of planar composition of Paul P. Cret's Museums. The purpose of this study is to look into the Beaux-Arts tradition in Cret's form and plan in accordance with French classicism that has followed Beaux-Arts system as planar axis and design methodology. The result of this study as follows: 1) Cret's architectural education is rational and scientific education way. 2) His design between structure and architecture is expressed to harmonize organic through studying possibility about form and structure system through revival of classic order and continuous communication with contemporary engineer as rationalistic viewpoint. 3) A Cret's design methode that emphasize compositional rank through disposition and distribution generalizes detail to reveal suitable prototype and feature of building.

7월 타오름달

김정곤,김희헌,황문찬,이도형,이숙진,김상혁,장영주,김별배,황찬진 새가정사 2018 새가정 Vol.712 No.-

과학교사 재교육의 개선방안

김정곤,김인호,정계준,김봉곤,구인선 한국과학교육학회 1991 한국과학교육학회지 Vol.11 No.1

The purpose of this study is in making a important way for in-service training of science teacthers. So the study was carried out to analyze the text books of in-service training school in Gyeongnam, Kyungpook, Junpook province and Pusan city and to survey teacher's opinion by questonnaires collected from teachers in Gyeongnam province. The result abtained are as follows; 1. The contents of the text books for in-service training course have very critical difference at each province. 2. Most of teachers wanted to get more updating information of the science and more comprehensive knowledge of higher level of science subject. 3. In order to obtain comprehensive basic concept, develop of inquiry ability, and promote of scientific attitude, the thinking experiment which is selected to experimental subjects of secondary school are need to be selected. 4. The new text-book model for in-service training course should be developed, and the finnancial support will be required to develop such a text.

水戸学における名分論に関する一考察

김정곤 한국일본사상사학회 2016 일본사상 Vol.0 No.30

This paper will review the Mitogaku a significant impact on the mind of the Shishi in the Late Tokugawa and Early Meji Period. For example, Shouin Yoshida and Takamori Saigou called the exponent are Seishisai Aizawa who was Mito Samurai and people influenced by "imperialism" thought of Toko Fujita.. The basis of the "imperialism" thought of such Mitogaku has the understanding for Justification theory of lord and vassal relations. I discussed this article through a representative book of Mitogaku from a point of view called Justification theory. I discussed it in this article around Dainihonnshi · Seimeiron · Jinshinkyoshusetsu. In addition, I confirmed it through Kanzou Uchimura who was Christ believer of the Meiji it was modern times, and how Justification theory acted. 本論文は、幕末·維新期における志士たちの精神に、大きな影響を与えた「水戸学」を検討したものである。例えば、幕末·維新の代表的人物と呼ばれる吉田松陰、西郷隆盛などは、水戸藩士であった会沢正志斎と藤田東湖の「尊王」思想によって感化された人である。このような水戸学の「尊王」思想の基には名分論的君臣関係に対する理解があると見て、その水戸学者の著書をとおして「名分論」という観点から論じた積りである。 ここで取り上げたのは、徳川光圀『大日本史』・藤田幽谷『正名論』・会沢正志斎『人臣去就説』であり、また、近代になって「名分論」はどのように働いたかを明治の基督信徒である内村鑑三を通して確認した。 まず、前者は共に徳川幕藩体制の下に、儒教的立場に基づきながら、『大日本史』は、朱子学的歴史思想に基づいて、日本の歴史において「皇統の正閏」と「人臣の是非」を「道徳的名分論」の基準で明らかにしようとした。幽谷の『正名論』は、儒教的「名分論」を日本的に読み替えて(「名分論」が直ちに「国体の尊厳」に繋がるので、臣下は条件なく絶対服従すべき)理解し、幕府は易姓のない万世一系の皇室を翼賛すべきことを唱えた。『人臣去就説』では、日本的君臣関係の理解を再び儒教伝統的解釈に戻した。要するに、「君君たらざれども臣臣たらざるべからず」・「忠臣は二君に仕えず」から「君臣義合」・「諫不聴去」へ変化であった。近代になって基督信徒であった内村鑑三は、儒教的君臣関係をキリスト教的に読み替え、君に対する忠誠を神に果たしたのであるが、彼の水戸学に打たれた、その心情とエトスは、キリスト教受容において土台として役割を果たしたといえよう。

Mechanochemical Ring-Opening Polymerization of Lactide

김정곤,온누리 한국고분자학회 2017 한국고분자학회 학술대회 연구논문 초록집 Vol.42 No.2