국내 밀 43개 품종에 대한 아그로박테리움 형질전환 효율성 검정

김재윤,이건희,이하늘,현도윤,Jae Yoon Kim,Geon Hee Lee,Ha Neul Lee,Do Yoon Hyun 국립한국농수산대학교 교육개발센터 2024 현장농업연구지 = Journal of practical agricultural resear Vol.25 No.4

본 연구는 국내 밀 43 품종에 대한 A. tumefaciens 형질 전환 효율을 검정하기 위해 GUS staining 분석을 수행한 것으로서 대부분의 밀 형질전환 연구가 특정 품종에 국한되어 있기 때문에 국내 장려 밀 품종에 대한 형질전환 효율에 영향을 미치는 조건에 대한 검정이 필요하다. 국내 밀 품종 중 32개에서 1개 이상의 조직에 염색된 신호가 관찰되었으며 4개 품종에서는 염색된 신호가 관찰되지 않았고 6개 품종에서 과도한 A. tumefaciens 성장이 관찰되었고, 7개 품종은 미성숙배의 크기 등의 이유로 GUS staining 분석이 불가능하여 추가 분석에서 제외하였다. 국내 밀 품종별 A. tumefaciens 감염 효율 비교 결과, 백립계 8개 품종 및 적립계 28개 품종에서는 평균적으로 유의한 차이가 없었다. 특히 조농밀, 조품밀, 새올밀, 조중밀, 새금강밀 등 적립계 품종에서 90% 이상의 높은 감염 효율이 관찰되었고, 전체 품종 중 22개는 50% 이상의 효율을 나타내었다. 본 연구를 통하여 조농밀 및 새올밀은 미성숙배에서 전체 조직에서 강한 GUS 발현으로 형질전환에 적합한 품종으로 나타났으며 금강 품종은 백립계에서 상대적으로 높은 발현을 보여 A. tumefaciens을 이용한 형질전환에 적합한 것으로 확인되었다. 추가적인 연구를 통해 품종의 조직배양 효율을 검정하고, 안정적인 GUS 발현 효율을 조사하는 것이 필요하며, 이를 통해 밀 형질전환에 이용 가능한 품종을 더욱 정밀하게 선발할 수 있을 것으로 판단된다. 해당 연구 결과는 국내 밀품종의 형질전환 효율을 높이기 위한 기초 자료로 활용 가능할 것이다. Agrobacterium-mediated transformation (AMT) is a method that allows for the stable integration of DNA fragments into the plant genome. Transgenic plants generated through AMT typically exhibit a lower copy number of the transgene compared to those induced by particle bombardment. Furthermore, AMT offers a straightforward and efficient approach for generating transgenic plants. While the transformation efficiency of wheat is comparatively lower than that of other monocot plants such as Rice (Oryza sativa L.) and Maize (Zea mays L.), the cultivars 'Bobwhites' and 'Fielder' are commonly employed for wheat transformation. To date, there have been no reported instances of successful development of transgenic plants using Korean wheat varieties through AMT. This study aims to assess the transformation efficiency of 43 Korean wheat cultivars using the GUS assay, with the goal of identifying suitable Korean wheat cultivars for AMT. The pCAMBIA1301 vector, carrying the β-glucuronidase (GUS) gene, was incorporated into Agrobacterium strain EH105. Following the inoculation of Agrobacterium into immature embryos, GUS assays were conducted 'Saeol', 'Jopum', and 'Jonong' showed 100% (the number of embryos showing GUS spots/the number of embryos used for AMT) among 43 cultivars. In addition, cultivars with more than 70% were 'Saekeumgang', 'Jojung', 'Tapdong', 'Anbaek', 'Dabun', 'Sugang', 'Keumgang', 'Jeokjung', 'Seodun', 'Joeun', 'Dajung', and 'Baekjung'. It seems that the 15 cultivars above showed the possibility of using AMT. On the other hand, 'Yeonbaek', 'Goso', 'Baekgang', and 'Johan' showed less than 20% and GUS spots were not observed in 'Gru', 'Gobun', 'Milseong', and 'Shinmichal-1'. This study explores transient GUS expression in Korean wheat cultivars seven days after AMT. The observed initial high efficiency of transient transformation suggests the potential for subsequent stable transformation efficiency. Korean wheat cultivars demonstrating elevated transient transformation efficiency could serve as promising candidates for the development of stable transgenic wheat.

원적외선의 인체작용메카니즘

김재윤,박영한,박돈목,박래준,Kim, Jae-Yoon,Park, Young-Han,Park, Don-Mork,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.2

Until now, it has not been well known for Far-infrared(FIR) how to affect to the human body. We introduced and presumed the mechanism of FIR based on molecular biology in this study, as below. The human body is composed of proteins which get easily changed by a thermal factor (about 42 $^{\circ}$C over). FIR with low temperature can deeply penetrate on the human body composed things without troublesome, since FIR has effectively operated on the human body at low temperature (35-40 $^{\circ}$C). When FIR penetrated on the human body, it would inhibit the abnormal genes and cells expression, and then information of DNA and RNA would be reexpressed for arranging DNA and RNA abnormal state. As FIR's receptors in the body, it colud be presumed that N-glycosyl linkage of purine and deoxyribose, RNA splicing process, and heat shock protein.

원적외선 최적화 방사유도 알고리즘과 프로그래밍

김재윤,박돈목,박영한,박래준,Kim, Jae-Yoon,Park, Don-Mork,Park, Young-Han,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.2

To take the Far-infrared(FIR) ray which is a optimized wavewlength and strength, at first, it is to be induced the characteristic algorithm and the computerized programing of FlR radiating materials. In this study, we induced that the formular of optimized FIR with physical, mathematical logic and theory, especially, Plank, Kirchhoff, Wien, Stefan-Boltzmann's logic and law. In the long run the formular was induced with mathematical integration. since we had to know the molecular wavelength. Base on the induced formular as above, we programmed the optimized FlR radiating computerized program, it would be useful to design semiconductor( VLSI) as the FlR instrument center control system.

원적외선이 관절염 흰쥐 모델에 미치는 영향과 관련 싸이토카인 예측 프로그래밍

김재윤,김진상,배성수,박래준,Kim Jae Yoon,Kim Jin-Sang,Bae Sung Soo,Park Rae Joon 대한물리치료학회 2003 대한물리치료학회지 Vol.15 No.1

The purpose of this study was to observe the effects of Far-infrared on mice rheumatoid arthritis induced by type II collagen. The author peformed several experimental tests which were the hematoxylin-eosin tissue stain, the hind paw thickness, and ELSIA of cytokines(TNF-$\alpha$, IL-1$\beta$). On day 14 and 21, FIR-Tx. group decreased in synovial membrane thickness more than control II group FIR-Tx group decreased in hind paw thickness more than control II group. In the ELISA test of TNF-$\alpha$ concentration, control II group significantly increased in the concentration more than normal and FIR-Tx. group on day 7, 14, and 21. In the ELISA test of IL-$\beta$ concentration, FIR-Tx. group decreased in the concentration more than control II group on day 14 and 21. With J's Linear Function Data Process Program, researchers can get the estimated the concentration or the hind paw thickness from this experiment. The estimated results would be a good guide to therapists and researchers, planning Far-infrared application on the rheumatoid arthritis. These results indicated that far-infrared irradiating appeared to reduce the concentration of TNF-$\alpha$ and IL-1$\beta$ at mouse model of autoimmune arthritis. It would be considered that Far-infrared has an effect on relieving rheumatoid arthritis.

Rabies Post-Exposure Prophylaxis of Overseas Travelers in the International Travel Clinic of the National Medical Center from 2006 to 2012, Korea

김재윤,Chang Hyu Lee,Youn Kyoung Won,Bum Sik Chin,Hyung Sik Shin,Jae Yoon Kim 대한감염학회 2014 Infection and Chemotherapy Vol.46 No.1

Background: Rabies is an acute fatal viral disease generally transmitted from infected animals to humans through bites. It is distributedworldwide. The number of Korean people traveling to rabies-endemic countries and being bitten by infected animals has beenincreasing recently. Therefore, we investigated international travelers who received rabies post-exposure prophylaxis (PEP) at the NationalMedical Center (NMC) and compared the data with those of other clinics. Materials and Methods: This study was a retrospective review of 106 patients who visited the International Travel Clinic of the NMCand received rabies PEP between July 2006 and December 2012. During that period, we used the Essen intramuscular regimen protocol. Complete rabies PEP was defined as 5 doses of rabies vaccination with or without rabies immunoglobulin (RIG) administrationaccording to the World Health Organization guidelines. Results: A total 106 cases documented within the period of 6 years were selected, including 10 children younger than 15 years and96 older than 15 years. The mean age of the patients who received PEP was 33.4 years. Of the patients, 53 were male and another53 were female. Most of the exposures occurred in Southeast Asia, predominantly from dog bites (71, 66.9%). The lower extremitieswere the most frequent site of exposure (37, 34.9%). All the patients began receiving rabies vaccination for prophylaxis after exposure,and 51 received rabies vaccination with RIG. Meanwhile, 74 cases (69.8%) initiated rabies vaccination overseas, but only 10 of themreceived RIG while overseas; the remaining 32 (30.2%) initiated rabies vaccination after returning to Korea. Within 7 days, all thechildren and 74 adults received their first rabies vaccination. Six adults initiated first rabies vaccination after 1 week. Eleven of the 106patients stopped PEP before 5 doses, among whom 4 (1 child and 3 adults) discontinued vaccination after confirming that the bitinganimal remained healthy throughout 10 days of observation. None of the patients had been previously vaccinated against rabies. Conclusions: Most of the overseas travelers who visited our clinic after being bitten by suspected rabid animals received appropriaterabies PEP. However, the interval between exposure and first rabies vaccination was often delayed. Tourists who planto travel in rabies enzootic regions need to be aware that prompt initiation of PEP is important to reduce the risk for developinghuman rabies.

원적외선이 흰쥐의 생존율에 미치는 영향

김재윤,박승규,김진상,박래준,Kim, Jae-Yoon,Park, Seung-Kyu,Kim, Jin-Sang,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.3

IR classified by wavelength three parts NIR, MIR. FIR. There is FIR which is radiated from healthy human body the wave length is 8-14m. The Sun's ray is composed of Infared(49%), Visible light(40%) and Ultra violet(11%), however the ray getting to the earth is FIR(60%), IR(20%), and UV(20%). Human beings has utilized FIR already from time immemorial. Hershel found out Infrared for the first time, in the Industrial Revolution the Infrared and FIR had been begun to use making products. FIR with low temperature can deeply penetrate on the human body composed things without troublesome, since FIR has effectively operated on the human body at low temperature (35-40 $^{\circ}$C). In this study, we experimented in the specific temperature FlR radiation intensity. water consumption rate, feed consumption rate. survival rate and mean of weight balance with FlR radiation instrument. According to the results, the FlR radiation to the mice assisted to increase the survival rate.

원적외선이 대장균의 생존율과 돌연변이에 미치는 영향

김재윤,박래준,Kim, Jae-Yoon,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.3

Hershel found out Infrared for the first time, in the Industrial Revolution the Infrared and FlR had been begun to use making products. FIR with low temperature can deeply penetrate on the human body composed things without troublesome. since FIR has effectively operated on the human body at low temperature (35-40$^{\circ}$C). When FIR penetrated on the human body, it would inhibit the abnormal genes and cells expression, and then information of DNA and RNA would be reexpressed for arranging DNA and RNA abnormal state. As FIR's receptors in the body, it colud be presumed that N-glycosyl linkage of purine and deoxyribose, RNA splicing process. and Heat shock protein. To radiate optimized FIR, in this study, we made the FIR radiation compound and instrument for unharming biological things. According to the results, the FIR radiation to E. coli., it did not induce genetic mutations and change the survival rate of E.coli.

COVID-19 팬데믹 상황에서 대학신입생의 회복탄력성: 자원 증진 프로그램의 개발

김재윤,이소영,박은영,마나,백준상,강민주,Kim, Jae Yoon,Lee, So Young,Park, Eunyoung,Mana, Mana,Baek, Joon Sang,Kang, Min Ju 대한가정학회 2022 Human Ecology Research(HER) Vol.60 No.2

Undergraduate freshmen are currently encountering numerous difficulties in adapting to college life due to the outbreak of the COVID-19 pandemic. Thus, several studies have underlined the need to develop interventions focused on undergraduate students' resilience during this situation. We adopted the service design method in identifying the difficulties faced by undergraduate freshmen during this pandemic, and also developed interventions for raising their resilience. The service design method is a person-centered approach which adopts the user's viewpoint in developing solutions, thereby enabling their requirements to be satisfied. Surveys and in-depth interviews revealed that undergraduate freshmen were experiencing psycho-emotional problems which were derived from schoolwork and college life. This is particularly the case with students who are experiencing greater levels of academic difficulty, and were insufficiently equipped with resources such as stress-coping strategies and social support. Four undergraduate freshmen were recruited online to test the effectiveness of a resource promotion program aimed at enhancing their resilience. This program has proved to be helpful in relieving daily/academic stress and in building relationships among freshmen, regarding which the participants showed a high level of satisfaction. The results were discussed by focusing on psycho-emotional difficulties and resources of college freshmen, as well as the effectiveness of the resource promotion program. Further research is required in order to verify the efficiency of the program and to confirm conclusions.

Far-Infrared의 발전사와 물리치료 적용에 대한 연구

김재윤,박래준,박영한,Kim, Jae-Yoon,Park, Rae-Joon,Park, Young-Han 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.1

The Sun's ray is composed of Infared(49%), Visible light(40%) and Ultra violet(11%), however the ray getting to the earth is FIR(60%). IR(20%), and UV(20%). Human beings has utilized FIR already from time immemorial. Hershel found out Infrared for the first time, in the Industrial Revolution the Infrared and FI R had been begun to use making products. In asia. also, asian already has made use of FIR to treat the body, product things and make warm the house in the winter, as it had been called Wull therapy, fermentation. and On-Dol system. In these days, with contemporary science FIR would be begun to clear up the implication in the human body and organic compounds. IR classified by wavelength three parts NIR, MIR, FIR. There is FIR which is radiated from healthy human body the wave length is 8-14 m. It is difficult to standardized the wavelength of IR, since each related associations have a different opinion, so we suggested ideal IR wavelength and biological, phsiotherapical, medical FIR wavelength.

원적외선이 자가면역성 관절염 흰쥐모델의 산화질소정량에 미치는 영향

김재윤,박규현,박영한,김진상,박래준,Kim Jae Yoon,Park Kyu Hyun,Park Young Han,Kim Jin-Sang,Park Rae Joon 대한물리치료학회 2002 대한물리치료학회지 Vol.14 No.2

OBJECTIVE: This study was Far infrared(FIR) irradiating to a rat collagen-induced autoimmune arthritis and observed nitric oxide level of spine. METHODS: Type II collagen(CII) in adjuvant was injected into rats on day 0. The results showed that rats injected with CII plus adjuvant had signs of autoimmune arthritis on day 14. Thereafter, Far infrared(FIR) irradiation was begun on day 15. The rats were irradiated FIR twice per day for 20 min each time. RESULTS: Seven days later(Day 22), the level of NO at FIR irradiated group(FRG; Arth+FIR) was decreased more than FIR-non irradiated group(FNRG; Arth). Twenty-one days later(Day 36), the level of NO at FRG was more closed to the control group's NO level than the FNRG. CONCLUSION: These results indicated that Far infrared irradiating appears to reduce the nitric oxide level at a rat model of autoimmune arthritis induced.