황련해독탕 추출액의 각막염 원인균에 대한 항균효과

김인수,서진종,김광곤,하동룡,신민구,김의수,전상윤,Kim, In-Soo,Seo, Jin-Jong,Kim, Kwang-Gon,Ha, Dong-Ryong,Shin, Min-Koo,Kim, Eui-Su,Jeon, Sang-Yun 대한한방내과학회 2014 大韓韓方內科學會誌 Vol.35 No.3

Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of $70{\mu}l$ dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from $70{\mu}l$ to $10{\mu}l$ at the same concentration. Results: 1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show any antimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentration but didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract $70{\mu}l$ was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.

Smoothe Handoff 지원을 위한 빠른 인증 알고리즘

김인수,김기천,김현곤,Kim, In-Su,Kim, Gi-Cheon,Kim, Hyeon-Gon 한국정보처리학회 2002 정보처리학회논문지 C : 정보통신,정보보안 Vol.9 No.1

IMT-2000망 핵심기술은 mobile IP를 이용하는 북미의 3G packet data system과 GSM망과 연계를 하는 유럽의 GPRS로 구분할 수 있다. 이러한 IMT-2000망 핵심기술은 글로벌한 로밍을 위해 Mobile IP도입을 추진하고 있다. Mobile IP상에서는 RADIUS나 DIAMETER같은 AAA 서버가 다이얼 업 컴퓨터의 인증, 허가 서비스를 제공하기 위해 사용되고 있는데, 이것은 MN에게 매우 중요하다. Mobile IP는 MN과 HA간에 강력한 인증을 요구하기 때문이다. 본 논문에서는 이러한 IMT-2000 환경에서 고려되고 있는 Smooth Handoff 기법에서 효과적인 AAA프로토콜의 적용에 관하여 논한다. IMT-2000 technologies are divided 3G packet data system that using mobile IP and GPRS that based on the GSM networks. These technologies Push introduce mobile IP to support seamless roaming. In mobile If environments, use AAA server such as RADIUS or DIAMETER to provide authentication service for dial-up computers. This factor is important for mobile nodes. Mobile If require strong authentication between mobile nodes and home agents. We propose application of AAA protocols for smooth handoff mechanism in IMT-2000 environments.

양계 농업인의 작업장 환경 및 개인보호구 착용 실태조사

김인수,김경란,이경숙,채혜선,김성우,Kim, Insoo,Kim, Kyung-Ran,Lee, Kyung-Suk,Chae, Hye-Seon,Kim, Sungwoo 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.6

Objectives: This study was conducted to investigate the actual condition of the farm work environment and personal protective equipment as part of the effort to improve livestock work for the safety and health of poultry farmers and provide basic data for establishing plans to improve and develop personal protective equipment. Methods: For this purpose, a questionnaire survey on general information about stables, the poultry work environment, accidents, the wearing of work clothes and personal protective equipment, and the level of awareness related to personal protective equipment was conducted among 148 poultry farmers. Results: As a result, it was found that poultry workplace environment was exposed to such risks as fine dusts; organic dusts; poisonous gases; odorous substances; chicken excrement; contact with chickens, bacteria or viruses; and accidents related to machine operation. Thirteen percent of respondents suffered severe respiratory diseases, and the most frequently injured sites due to accidents were the hands (25.7%), knees (23.8%), arms (17.3%), and head (10.9%). The most frequent type of accident was collisions between the body and obstacles or machinery during movement (36.4%), followed by erroneous machine operation such as feeders and electric shocks (8.5%). Regarding the wearing of work clothes and personal protective equipment, 51.7% of the respondents wore worn-out clothing or everyday clothes, whereas only 32.0% wore work clothes. The percentage of farmers who wore proper protective equipment for the work environment during poultry work was 48.4%. The most frequently used type of protective equipment was boots (38.9%), followed by mask (36.7%), gloves (36.3%), appropriate work clothes (22.6%), quarantine clothes (17.6%), helmets (13.4%), and goggles (12.6%). The rate of wearing goggles was low because they were considered inconvenient and lowered work efficiency. Furthermore, they purchased everyday products available on the market for their personal protective equipment which were not appropriate for maintaining safety in an actual harmful environment and its consequent risks. As a result of the survey of the awareness level related to personal protective equipment, their levels of awareness of accidents and attitude proved to be average or higher, but the practice of wearing protective equipment and the level of knowledge and management of personal protective equipment were lower. Conclusion: This survey found that the wearing status of personal protective equipment among poultry farmers was insufficient even though they were exposed to risks. Most respondents were aware of the necessity of wearing personal protective equipment and of the potential for accidents, but they did not wear proper protective equipment. Their wearing rate was low due to a lack of knowledge about protective equipment, as well as the inconvenience of wearing it. Therefore there is a need to improve and develop specialized personal protective equipment for respiration, hands, and eyes, as well as work clothes that can protect farmers from major harmful matter that is generated in the poultry workplace. Based on the results of this investigation, we will conduct further studies on the required performance and design directions of personal protective equipment while collecting more objective data through field-oriented assessments.

藥水 수질 特性에 관한 연구 -전남지역을 중심으로-

김인수,하훈,서원술,배주순,문희,박철웅,오은하,이소영,김명혜,kim, I.S.,Ha, H.,Seo, W.S.,Bae, J. S.,Mun, H.,Park, C.U.,Oh, E.H.,Lee, S.Y.,Kim, M.H. 한국환경보건학회 1998 한국환경보건학회지 Vol.24 No.1

This study was performed to investigate the Natural mineral water quality at 47 sites in Chonnam area, samples were collected spring, summer, fall, winter for a seasonal variation in 1996. The results obtained were summarized as follows 1. The mean of F, pH, HCO$_3$, NO$_3$ -N were 0.19 mg/l, 6. 9, 34.99 mg/l, 1.40 mg/l and those of Na, K, Ca, Mg were 12.1 mg/l, 1.2 mg/l, 7.7 mg/l, 2.1 mg/l respectively. 2. The contents of F were reasonable at Multonggol as 1.35 mg/l and Yongbayui as 0.57 mg/l respectively, but there is no detected at Sidong and detected below 0.1 mg/l at Gubongsan, Hansansa area. 3. According to the geological effect, the content of F was showed higher in granite than in tuff, and index of pH was higher in tuff than in andesite, and content of HCO$_3$ was higher in andesite than in granite. 4. The K and O values were used as index of healthy and delicious water. As a result, thirtyfive sites(74.5%) was decided as healthy and delicious water. 5. The contents of NO$_3$-N were detected over 5 mg/l at Sukchonsa, Gajanggol, Yudalsan, Muchang, Kisan.

Hydrophobic Sites on the Protein Surfaces of the Pr and Pfr Forms of Phytochrome

김인수,송필순,Kim, In-Soo,Song, Pill-Soon Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.2

Phytochrome의 활성형인 Pfr과 불활성형인 Pr의 단백질 표면에 있는 소수성 부위를 $\alpha$-chymotrypsin에 의한 부분가수분해와 SDS에 의한 변성으로 연구하였다. Phytochrome을 효소와 60:1 (w/v)의 비율로 가수분해 할때 Pr형의 660 nm 흡광도는 20%가 감소하는 반면 Pfr 형의 730 nm 흡광도는 완전히 소멸하였다. $\alpha$-chymotrypsin의 가수분해물을 SDS-gel 전기영동으로 검정한 peptide 양상으로 보아서 Pr형이 가수분해속도가 빠르지만 단백질표면의 소수성 부위 는 Pfr 형에서 더욱 다양하게 분포되어 있었다. $\alpha$-Chymotrypsin 가수분해의 초기 생성물인 chromopeptide는 non-chromopeptide보다 가수분해 속도가 느리게 나타났다. Pfr 형이 Pr형에 비하여 가수분해 속도가 느림에도 불구하고 흡광 spectrum이 급격히 변화하는 것은 Pr 형에서 Pfr형으로 광전환 할때 단백질과 색소의 결합주변에 소수성 부위가 생성되었기 때문이고 이 소수성 부위는 phytochrome의 활성부위와 관련이 있을 것으로 생각되었다. The nature of hydrophobic sites on the protein surfaces of the Pr and Pfr forms of phytochrome (118 kDa) has been studied by spectroscopic and electrophoretic properties of phytochrome that was partially digested with $\alpha$-chymotrypsin. The enzyme digestion bleached the Pfr spectrum completely at a 1:60 (w/v) enzyme to substrate ratio, while the Pr absorbance was decreased by 20%. SDS-gel electrophoresis of the $\alpha$-chymotrypsin-digested phytochrome showed that, at an early stage of digestion, the rate of hydrolysis was faster in the Pr form and the electrophoretic pattern of polypeptide digests was more complicated in the Pfr form than in the Pr form. The chromopeptide domain was more resistant to $\alpha$-Chymotrypsin digestion than the nonchromophore domain. The pronounced susceptibility of the Pfr spectrum to $\alpha$-Chymotrypsin was considered to be the action of the enzyme on hydrophobic site(s) at the chromophore vicinity that was newly developed by phototransformation from the Pr form to the Pfr form.

Spectroscopic Characterization of Pr and Pfr Forms of Phytochrome Denatured by Urea

김인수,송필순,Kim, In-Soo,Song, Pill-Soon 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.1

순수한 phytochrome을 요소로 변성시킬 때 나타나는 흡광도, CD 및 형광동의 분광학적인 변화를 관찰하여 활성형인 Pfr과 비활성형인 Pr간의 분자 구조의 차이를 연구하였다. Phytochrome을 요소로 처리할 때 가시광선 (300~800nm파장)에서의 CD와 흡광도는 Pfr형이 Pr형에 비하여 큰 변화를 받고 있으나, 자외선 (200~300nm파장)에서의 흡광도, CD 및 형광의 변화는 두 형태간에 동일하였다. 가시광선에서의 Pr형과 pfr형의 감수성 차이는 요소에 대하여 색소-단백질의 상호작용이 서로 다른 영향을 받기 때문이다. 그러나 peptide의 흡광파장인 자외선에서의 결과는 두 형태간에 요소에 대한 단백질의 변성 정도 및 단백질의 구조에는 차이가 없음을 나타내고 있다. Phytochrome을 요소로 부분변성시켰을 때는 청색광(380nm 파장)에 의한 Pr형에서 Pfr형으로의 광전환이 촉진된다. The molecular nature of urea-treated phytochrome has been studied spectroscopically using a highly purified large phytochrome. The spectral informations of absorption, CD and fluorescence are extended to the far-UV region. The absorption and CD spectra in the chromophoric region showed that the Pfr spectra were perturbed more drastically than the Pr spectra toward urea denaturation. The CD in the peptide absorption region, and absorption and fluorescence emission of tryptophan / tyrosine residues of phytochrome indicated that the conformation of the native state was the same for both forms of phytochrome, and that susceptibilities of protein unfolding remained the same for the both forms during denaturation. These observations suggest that protein chromophore interaction sites of the both forms have different sensitivities to urea denaturation, but that the conformational susceptibilities of their protein moieties to urea are the same. Partial denaturation of phytochrome with 3M urea oriented the chromophore to have a higher phototransformation rate by blue light irradiation.

Cool Asphalt Pavement(열섬완화 아스팔트 포장) 기술

김인수,한의석,이광호,Kim, In-Su,Han, Ui-Seok,Lee, Gwang-Ho 한국도로학회 2008 한국도로학회지 Vol.10 No.1

고자기학(古磁氣學)의 방법(方法) (I)

김인수,Kim, In-Soo 대한자원환경지질학회 1984 자원환경지질 Vol.17 No.2

For the discussion of palaeogeomagnetic field direction and the movement and rotation of geotectonic blocks it is prerequisite to eliminate the secondary remanent magnetization component out of the NRM vector of given rock samples. Among various techniques alternating field and thermal demagnetization methods are most easily applicable and most widely used ones. Physical principle underlying these methods is the concept of relaxation time: Either with an action of external magnetic field or with an elevated temperature relaxation time of magnetic minerals can be drastically shortened. It has been furthermore shown experimentally that the secondary remanent magnetization can be more easily demagnetized than the primary remanent magnetization. Through careful stepwise demagnetization it should also be possible to discriminate the kind of various remanent magnetizations. In addition to the introduction to the underlying physical principles and experimental results of the alternating field and thermal demagnetization this paper gives various practical tips in earring out the demagnetization experiments. Each alternating field and thermal demagnetization instrumentation which is easily adaptable to domestic palaeomagnetic laboratories is also introduced. This paper provides a compact and practical introduction to the methods in palaeomagnetism and would be of interest to present workers and to those who want to embark on research in this field.

MOTIVATION

김인수,Kim I.S. 대한간호협회 1982 대한간호 Vol.21 No.4

Urea 에 의해 변성된 Phytochrome 의 분광학적인 성질

김인수,송필순 ( In Soo Kim,Pill Soon Song ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1

The molecular nature of urea-treated phytochrome has been studied spectroscopically using a highly purified large phytochrome. The spectral informations of absorption, CD and fluorescence are extended to the far-UV region. The absorption and CD spectra in the chromophoric region showed that the Pfr spectra were perturbed more drastically than the Pr spectra toward urea denaturation. The CD in the peptide absorption region, and absorption and fluorescence emission of tryptophan / tyrosine residues of phytochrome indicated that the conformation of the native state was the same for both forms of phytochrome, and that susceptibilities of protein unfolding remained the same for the both forms during denaturation. These observations suggest that protein chromophore interaction sites of the both forms have different sensitivities to urea denaturation, but that the conformational susceptibilities of their protein moieties to urea are the same. Partial denaturation of phytochrome with 3M urea oriented the chromophore to have a higher phototransformation rate by blue light irradiation.