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김익영,정대원,Eun Hae Kim,Tae Soo Kim,Youn Wook Chung,김현 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.1
Whereas the levels of other selenoproteins in the brain decrease when selenium is deficient, the level of seleno-protein W (Se-W) is maintained, suggesting that it has a critical role in the brain. Previously, we reported that Se-W is a GSH-dependent antioxidant [Jeong et al. (2002)]. In this study, the expression of Se-W and thio-redoxin (Trx) in the brain and during embrynic devel-opment was analyzed by an in situ hybridization tech-nique. Se-W mRNA was highly expressed in the cortex, dentate gyrus, and hippocampus of postnatal rat brains, and in the spinal cord and brain of developing embryos. In contrast, Trx mRNA was highly expressed in the cerebellum, olfactory bulb, and dentate gyrus of postna-tal rat brains, and in the liver, telencephalon, and back muscle of developing embryos. Thus these two antioxi-dant proteins have different and non-overlapping ex-pression patterns. The distribution of Se-W suggests that it plays an important role as an antioxidant in the developing brain and embryo.
지연태,김익영,이세영 ( Youn Tae Chi,Ick Young Kim,Se Yong Lee ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1
As a part of the host development for a amplified recombinant E. coli trp operon (R_6K::Mu-trpL(△att)trpE^(FBR)) for the production of tryptophan, the 5-methyltryptophan-resistan mutation (5-MT^r), tryptophan aminotransferase-deficient mutation (tat^-) and L-serine deaminase-deficient mutation (ssd^-) were introduced into the K. pneumoniae host cell and their effects on the expression of the trp operon and production of tryptophan were examined. The 5-MT^s mutation derepressed the expression of the recombinant E. coli trp operon and both tat^- and ssd^- mutation increased the activities of anthranilate synthetase and tryptophan synthetase as well as the producation of tryptophan in K. pneumoniae.
김익환,김익영,최태부,김홍진,박홍우,최연숙,Doo Young Lee 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.1
The stabilization of optimum pH for cells can cause a higher erythropoietin (EPO) production rate and a good growth rate with the prolonged culture span in recombinant Chinese hamster ovary (r-CHO) cells. Our strategy for stabilizing the optimum pH in this study is to reduce the lactate production by adding sodium lactate to a culture medium. When 40 mM sodium lactate was added, a specific growth rate was decreased by approximately 22% as compared with the control culture. However, the culture longevity was extended to 187 h, and more than a 2.7-fold increase in a final accumulated EPO concentration was obtained at 40 mM of sodium lactate. On the condition that caused the high production of EPO, a specific glucose consumption rate and lactate production rate decreased by 23.3 and 52%, respectively. Activity of lactate dehydrogenase (LDH) in r-CHO cells increased and catalyzed the oxidation of lactate to pyruvate, together with the reverse reaction, at the addition of 40 mM sodium lactate. The addition of 40 mM sodium lactate caused the positive effects on a cell growth and an EPO production in the absence of carbon dioxide gas as well as in the presence of carbon dioxide gas by reducing the accumulation of lactate.
지연태,김익영,이세영,Chi, Youn-Tae,Kim, Ick-Young,Lee, Se-Yong 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1
트립토판 생산성 증대를 위한 균주 개발의 일환으로 숙주 박테리아인 Klebsiella pneumoniae의 5-methyltryptophan 내성주(5-$MT^r$), tryptophan aminotransferase 결손주($tat^-$) 및 L-serine deaminase의 결손주($ssd^-$)를 얻고, 또한 이들 변이가 증폭된 재조합 E. coli trp operon ($R_6K::Mu-trpL({\Delta}tt)trpE^{FBR}$)의 발현에 어떻게 영향을 미치는지 조사하였다. K. pneumoniae에서 5-$MT^r$, $tat^-$, $ssd^-$ 돌연변이는 재초합 E. coli trp operon의 발현을 증가시켰으며, trp operon의 최종산물인 트립토판의 생산성도 증가시켰다. As a part of the host development for a amplified recombinant E. coli trp operon ($R_6K::Mu-trpL({\Delta}att)trpE^{FBR}$) for the production of tryptophan, the 5-methyltryptophan-resistan mutation (5-$MT^r$), tryptophan aminotransferase-deficient mutation ($tat^-$) and L-serine deaminase-deficient mutation ($ssd^-$) were introduced into the K. pneumoniae host cell and their effects on the expression of the try operon and production of tryptophan were examined. The 5-$MT^r$ mutation derepressed the expression of the recombinant E. coli trp operon and both $tat^-$ and $ssd^-$ mutation increased the activities of anthranilate synthetase and tryptophan synthetase as well as the producation of tryptophan in K. pneumoniae.