Isepamicin의 호기성 그람음성간균에 대한 시험관내 감수성

김의종,백승호 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.3

목적 : Isepamicin은 gentamicin B에서 유래된 반합성약제로서 amikacin과 유사한 임상치료 효과를 보이며, 독성이 비교적 낮다고 보고되었다. 본 연구에서는 대학병원에서 분리한 호기성그람음성간균증 gentamicin에 대해 내성인 균주를 대상으로 isepamicin의 시험관내 감수성을 amikacin과 비교하여 보았다. 방법 : 입원환자로부터 분리한 호기성 그람음성간균중에서 gentamicin에 대해 내성을 보인 총 201균주를 선택하였으며, 이 균주들을 다시 microdilution 법으로 gentamicin에 대한 최소억제 농도가 16㎍/ml이상인 것을 확인하고 사용하였다. 각 균주의 isepamicin과 amikacin에 대한 최소억제농도(MIC)는 미국의 National Committee for Clinical Laboratory Standards의 표준안에 따라 microdilution 법으로 측정하였다. 결과 : Gentamicin에 대해 내성인 E. coli, K. pneumoniae, E. cloacae와 S. marcescens에 대한 isepamicin의 MIC는 amikacin과 비교하여 낮았고, P. aeruginosa에 대한 MIS분포는 두 항생제간에 큰 차이는 발견되지 않았으며, P. miranilis는 두항생제에 대해 모두 감수성이었으며, gentamicin-resistant S. malitophilia에 대하여는 두 항생제에 대해 모두 내성이었고, A. baumanii의 경우에는 isepamicin보다 amikacin에서 MIC가 낮았다. 결론 : Isepamicin은 E. coli, K. pneumoniae, E. cloacae S. marcescens에 대하여 amikacin보다 우수한 항균력을 갖고 있으나, P. aeruginosa, P. mirabilis와 S. maltophilia에 대한 항균력은 두 항생계간에 차이가 없었으며, A. baumanii의 경우에는 amikacin보다 항균력이 떨어짐을 알 수 있었다. Background : Isepamicin is a semi-synthetic antibiotic derived from gentamicin B which is expected to have a clinical efficacy similar to that of amikacin but with less toxicity. In this paper we describe the in vitro activity of isepamicin and compare it to amikacin against gentamicifi-resistant aerobic gram-negatme bacilliisolated from a university hospital. Methods : 4 total 201 gentamicin-resistant gram-negative bacilli isolated from inpatients were used during this study and minimal inhibitory concentrations (MICs) of all isolates mere 16 ㎍/mL or more by microdilution method. Susceptibility testing to determine MICs was performed by the broth microdilution method as recommended by the National Committee for Clinical Laboratory Standards. Results : The M1C ranges of isepamicin for gentamicin-resistant E. coli, K. pneumoneae, E. cloacae, and S. marcescens were lomer than that of amikacm. The MIC range of P. aeruginosa did not show significant difference betn-een both antibiotics. All of gentamicm-resistant P. miabilis were susceptible to isepamicin and amikacin, and all of S. maltophilia were resistant to both antibiotics. The MICs of isepamicin for A. baumanii were higher than that of amikacin. Conclusion : In summary, the in vitro activity of isepamicin is slightly greater than amikacin against E. coli, K. pneumoniae, E. cloacae, and S. marcescens. There IS no significant differences between isepamicin and amikacin is antibacterial activity against P. aerugznosa, P. mirabilis, and S. maltophilia. The in vitro activity of isepamicin is slightly lesser than amikacm against A. baumanzi..

주요 임상분리센균에 대한 moxifloxacin과 ciprofloxacin의 최소억제농도

김의종,김하영,이상원 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

목적 : Moxifloxacin에 대한 국내 임상 분리 균주의 감수성 여부를 팡가하기 위하여 임상 환자에서 흔히 분리되는 통성 혐기성 세균을 대상으로 최소억제농도를 측정하였으며, 대조 항생제로는 ciprofloxacin을 사용하여 그 결과와 서로 비교하였다. 방법 : 시험 균종은 1999년 서울대학교병원 임상병리과로 세균배양이 의뢰된 혈액, 객담, 소변 등 임상 검체에서 분리한 균주로서 임상 환자에서 흔히 분리되는 통성 혐기성 세균 총 507주를 대상으로 하였다. 미국임상검사표준화협회(NCCLS)에서 권장하는 방법에 따라 S. pneumoniae와 H. influenzae를 제외한 모든 균종은 한천희석법으로 MIC를 측정하였으며, S. pneumoniae와 H. influenzae는 microdilution broth법으로 MIC를 측정하였다. 결과 : Staphylococcus spp.와 Enterococcus spp.에서 moxifloxacin에 대한 MIC가 ciprofloxacin에 대한 MIC보다 전반적으로 낮았다. PSSP의 경우 moxifloxacin에 대한 MIC가 cirpofloxacin의 MIC보다 4-8배 낮았다. PRSP의 경우 moxifloxacin에 대한 MIC는 2㎍/㎖인 한 균주를 제외하고는 모든 균주가 0.06-0.125㎍/㎖으로 감수성이었다. Ciprofloxacin에 대해 감수성을 보인 PRSP의 moxifloxacin에 대한 MIC는 ciprofloxacin의 MIC보다 4-8배 낮았다. E. coli, K. pneumoniae와 E. cloacae에서는 ciprofloxacin의 MIC가 moxifloxacin보다 낮았다. P. aeruginosa의 moxifloxacin에 대한 MIC가 ciprofloxacin의 경우보다 4-8배 높았다. A. baumanii와 S. maltophilia의 moxifloxacin에 대한 MIC가 ciprofloxacin의 경우보다 2-8배 낮았다. H. influenzae의 moxifloxacin에 대한 MIC_(90)는 0.015㎍/㎖ 이하이었고, ciprofloxacin에 대한 MIC도 이하 동일한 결과가 관찰되었다. 결론 : Ciprofloxacin은 Enterobacteriaceae와 Pseudomonas spp.에 대하여 항균력이 높고, moxifloxacin은 페니실린 내성 유무에 관계없이 S. pneumoniae에 대하여 항균력이 뛰어나다. 우리 나라에서 moxifloxacin을 사용한 후, S. pneumoniae에 대한 moxifloxacin의 MIC 분포가 내성 쪽으로 기울어지는 지를 계속적으로 모니터링해야 할 것이다. Background: Minimal inhibitory concentrations (MICs) of facultative anaerobic bacteria isolated from patients were determined for the in vitro susceptibility of Korean clinical isolates against moxifloxacin and compared with MICs of ciprofloxacin. Methods: Total 507 strains isolated from blood, sputum, or urine of patients at Seoul National University Hospital in 1999 were examined by agar dilution or microdilution broth method according to the recommendations of National Committee for Clinical Laboratory Standards. Results: The MICs of moxifloxacin against Staphylococcus spp. and Enterococcus spp. were lower than those of ciprofloxacin. For penicillin-susceptible S. pneumoniae moxifloxacin showed 4 to 8-fold lower MIC than ciprofloxacin. All of penicillin-resistant S. pneumoniae were susceptible to moxifloxacin (MICs 0.06-0.125 ㎍/㎖). except one strain (MIC 2 ㎍/㎖). The MICs of moxifloxacin against ciprofloxacin-susceptible. penicillin-resistant S. pneumoniae were 4 to 8-fold lower than those of ciprofloxacin. Against E. coil. K. pneumoniae. and E. cloacae. MICs of ciprofloxacin were lower than those of moxifloxacin. Against P. aeruginosa, moxifloxacin was less active than ciprofloxacin. Moxifloxacin was 2 to 8-fold more active than ciprofloxacin against A. baumanii and S. maltophilia. Against H. influenzae. moxifloxacin (MIG_(90) ≤0.015㎍/㎖) was moxifloxacin was as active as ciprofloxacin. Conclusion: Moxifloxacin was more active against gram-positive bacteria, especially penicillin-resistant S. pneumoniae. Ciprofloxacin was more active against Enterobacteriaceae and P. aeniginosa. The continuous monitoring for the change of susceptibility of moxifloxacin against S. pneumoniae is needed after using moxifloxacin in Korea.

광범위베타락탐아제 생성 Escherichia coli와 Klebsiellapneumoniae의 piperacillin/tazobactam에 대한 시험관내 감수성

김의종 대한감염학회 2008 Infection and Chemotherapy Vol.40 No.1

Minimal inhibitory concentrations (MIC) of piperacillin/tazobactam were determined on 20 clinical isolates of extended-spectrum β-lactamase (ESBL)-producing E. coli and 30 isolates of ESBL- producing Klebsiella pneumoniae. MIC50 and MIC90 for ESBL-producing E. coli were 8/4 µg/ml and 256/4 µg/mL, respectively. MIC50 and MIC90 for ESBL-producing K. pneumoniae were 8/4 µg/mL and >512/4 µg/mL, respectively. The susceptibilities of ESBL-producing E. coli and K. pneumoniae to piperacillin/tazobactam were 80% and 60%, respectively. Of 20 ESBL-producing E. coli strains, 11 (55%) were TEM-and CTX-M-positive, and SHV-negative. Of 30 ESBL-producing K. pneumoniae strains, ten (33%) were PCR positive for SHV and negative for TEM and CTX-M.

정상 한국인의 혈중 Glucagon 의 동태에 관한 연구

김의종,이홍규,고창순,홍기석,김병국,정준기 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1

It is well known that glucagon, like insulin, is very important in the moment-to-moment control of the homeostasis of glucose, and of amino acids. Glucagon has been shown to have potent glycogenolytic, gluconeogenic and lipolytic activities. Attention to its role in the pathogenesis of diabetes mellitus and hypoglycemia has been also advanced recently. To evaluate the diurnal variation of plasma glucagon concentration, we measured serum glucose, insulin, and plasma glucagon every 30 minutes or every hour in 7 normal Korean adults. Results were as follows: 1) Although plasma glucagon concentration showed wide individual variations, it had a tendency to decrease after meals. After lunch and dinner, plasma glucagon concentration had gradual1y declined and reached its nadir at postprandial 2-2.5 hours. The minimal level of plasma glucagon was at 4 A.M. 2) Serum insulin:plasma glucagon ratios were increased promptly after meals. Especially after lunch, its peak was prominent (3.65±1. 95). The minimal level of serm insulin:plasma glucagon ratio appeared at 6 AM.

혈액에서 분리된 세균의 항생제감수성 : Vitek System을 이용한 최소억제농도측정 Minimal Inhibitory Concentration Determined by Vitek System

김의종,김선주,신보문,박명희,조한익,김상인 대한화학요법학회 1988 대한화학요법학회지 Vol.6 No.1

Minimal inhibitory concentrations of total 148 strains isolated from blood culture at Seoul National University Hospital were determined by Vitek System. 90% MIC of gram-negative bacteria against amikacin was 2-16㎍/㎖, and those against beta-lactams was > 16㎍/㎖ in general, Except Salmonella spp,. 90% MIC against chloramphenicol and tetracycline was >8㎍/㎖. Range of 90% MIC against vancomycin was ≤.5-4㎍/㎖ in case of garm-positive bacteria, except Enterococcus spp. which showed 90% MIC as >16㎍/㎖. Against ampicillin, 90% MIC of Staphylococcus spp. was >16㎍/㎖, but those of Streptococcus spp. was ≤.25-2㎍/㎖. Streptococcus viridans group showed 90%MIC against penicillin as ≤.03㎍/㎖. Susceptibility of gram-negative bacteria was generally 98% against amikacin, and those of gram-positive bacteria was 80% against vancomycin. 34% of Staphylococcus spp. was resistant of oxacillin. These results may be useful for the primary selection and the dosage determination of antibiotics in the treatment of patients with bacteremia.

강해설교에 대한 개념이해와 중요성

김의종 장로회신학대학교 세계선교연구원 1998 개혁 신학 Vol.10 No.-

SM-101외 12종의 항생물질에 대한 항균력 시험

김의종,석종성 대한화학요법학회 1992 대한화학요법학회지 Vol.10 No.1

SM-101, SP-102, SA-103, SB-104, SC-105, and SD-106, Korean products, were tested for its antimicrobial effect I comparison with those of ampicillin, amoxicillin, cefazolin, cefamandole, cefotaxime, sulbactam, and piperacillin. Minimal inhibitory concentrations of total 286 clinical isolates were determined by microdilution broth method and all of antimicrobials were tested at concentration from 0.125 to 128 ㎍/ml. Ninety percent of coagulase-negative staphylococci were inhibited at 4 ㎍/ml of SB-104, SM-101 and SB-104 showed relatively lower MIC range against methicllin-susceptble Staphylococcus aureus. Ninety percent of ampicillin-susceptible E. coli were inhibited at 2 ㎍/ml of SP-102 and SC-105. Fifty percent of P. aeruginosa were inhibited at 8㎍/ml of SP-102 and SC-105. Compared to ampicillin, cefazolin, cefotaxime, and sulbactam, SM-101 and 5 other antimicrobials showed higher activities against Bacteroides spp, and SA-103 showed the lowest range of MIC against Bacteroides spp. Among 6 antimicrobial products, SD-106 showed the highest MIC range against all of clinical isolates.

임상분리 효모균주에 대한 항진균제의 시험관내 감수성양상

김의종,김재석,정현진 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.3

배경 : 최근 진균감염증 환자의 수가 증가하면서 항진균제의 사용량도 증가하였고, 항진균계에 대한 내성균주의 출현이 우려된다. 따라 현재 임상에서 가장 많이 사용하는 항진균제인 amphotericin B와 fluconazole에 대한 감수성양상을 파악할 필요가 있다. 방법 : 서울대학교병원 환자의 검체에서 분리한 총 200주의 임상분리효모균을 대상으로 National Committee for Clinical Laboratory Standards에서 권장하는 microdilution broth법을 실시하여 균종별로 amphotericin B 및 fluconazole의 최소억제농도분포를 조사하였다. 결과 : Candida albicans의 fluconazole과 amphotericin B에 대한 최소억제농도분포를 보면 MIC_(50)이 각각 0.5 ug/mL과 0.25 ug/mL이었으며, MIC_(90)는 각각 >16 ug/mL과 0.5 ug/mL이었다. Candida albicans의 경우 amphotericin B에 대한 최소억제농도가 2 ug/mL이상인 균주는 관찰되지 않았다. Torulopsis glabrata, Cnndida krusei와 Trichosporon beigel??의 경우 fluconazole에 대한 최소억제농독 amphotericin에 비하여 높았다. Candida albicans의 fluconazole에 대한 내성율은 15.3%이었다. 결론 : C. albicans에서 fluconazole에 대한 내성율이 높고, 일부 효모균은 fluconazole에 대해 내성이므로, 진균감영증 환자를 적절하게 치료하기 위하여 임상미생물검사실에서는 분리된 균주를 정확하게 동정하고 항진균제감수성검사를 실시해야 한다. Background : Recently the number of patients with fungal infection has been increased and the antifungal agents have been prescribed more than the past. The emergence of the resistant strain against antifungal agents may be expected. It is necessary that antifungal susceptibility patterns of amphotericin B and fluconazole, those are commonly used by clinicians in Korea, are evaluated. Methods : The minimal inhibitory concentrations of total 200 strains of yeast isolated from Seoul National University Hospital were measured against amphotericin B and fluconazole by microdilution broth method of National Committee for Clinical Laboratory Standards. Results : MIC?? and MIC?? of Candida albicans against fluconazole and amphotericin B were 0.5 ug/ml and 0.25 ug/ml, >16 ug/ml and 0.5 ug/ml, respectively. There was no strain of C. albzcans with MIC of more than 1 ug/ml against am photericin 3. The MICs of Torulopszs glabrata, Candida krusez, and Trichosporon beigelrz against fluconazole were higher than those against amphotericin 3. The resistant rate of C. albzcans against fluconazole was 15.3%. Conclusion : The resistant rate of C. albicans IS high against fluconazole and some species of yeast are resistant to fluconazole. It is recommended that the exact identification and antifungal susceptibility tests are performed in clinical microbiology laboratory for the adequate treatment of patients with fungal infection.

수치 계산을 통한 다목적 BIPV 시스템의 운전방법에 관한 연구

김의종,서승직,김헌중 한국태양에너지학회 2006 한국태양에너지학회 논문집 Vol.26 No.1

The Multipurpose BIPV System(MBIPVS) was evaluated as an effective passive system through analyzing the thermal performance and the efficiency of PV power generation in the previous papers. To achieve the performance better, the operation method should be determined by considering physical conditions in each occasion. Thus, we choosed the reference operation methods in each season set by the overview of the meteorological data for last 6 years, In-choen, and compared them with the various alternatives that we had made up with for improving thermal performance. The results from adopting various alternatives on MBIPVS showed that the appropriate operational model would be effective to the energy savings ; we could reduce the total loads 1,051.0[kWh] in summer and 108.9[kWh] in winter.Keywards : 다목적 BIPV 시스템(MBIPVS), 운전모드(Operation Mode), 부하계산(Load Calculation)기 호 설 명

결핵균 염색체 DNA 1. 6-kilobase의 염기배열분석에 관한 연구

김의종,이남용,이명희,강성하 대한임상병리학회 1995 대한임상병리학회지 Vol.15 No.4