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Photo-reactive natural polymer derivatives for medical application
김은혜,한가득,노승현,김재원,이재관,Yoshihiro Ito,손태일 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-
Biomaterials should be biocompatible and biodegradable. Many researches had been studied to upgrade biomaterials to deliver various types of drugs. In this respect, natural polymers have been catching on as a biomaterial. To immobilize drugs using natural polymer, photo-immobilization method has been designed. This method has several advantages. For photo-immobilization, photo-reactive functional group should be introduced to natural polymer. According to purpose of application, you could choice one of the photo-reactive functional group. Finally, many types of chemically modified natural polymers, which are photo-reactive natural polymer derivatives, have been prepared and studied to apply in medical field.
차세대 염기서열 분석법을 이용한 15개 상염색체 STR의 염기서열 생성 및 유전자형 분석
김은혜,양인석,정상은,신경진,양우익 대한법의학회 2014 대한법의학회지 Vol.38 No.2
Recently, next generation sequencing (NGS) has received attention as the ultimategenotyping method to overcome the limitations of capillary electrophoresis (CE)-based short tandem repeat (STR) analysis, such as the limited number of STR locithat can be measured simultaneously using fluorescent-labeled primers and the maximumsize of STR amplicons. In this study, we analyzed 15 autosomal STR markersvia the NGS method and evaluated their effectiveness in STR analysis. Using maleand female standard DNA as single-sources and their 1:1 mixture, we sequentiallygenerated sample amplicons by the multiplex polymerase chain reaction (PCR)method, constructed DNA libraries by ligation of adapters with a multiplex identifier(MID), and sequenced DNA using the Roche GS Junior Platform. Sequencing datafor each sample were analyzed via alignment with pre-built reference sequences. Most STR alleles could be determined by applying a coverage threshold of 20% forthe two single-sources and 10% for the 1:1 mixture. The structure of the STR in eachallele was accurately determined by examining the sequences of the target STRregion. The mixture ratio of the mixed sample was estimated by analyzing the coverageratios between assigned alleles at each locus and the reference/variant ratiosfrom the observed sequence variations. In conclusion, the experimental method usedin this study allowed the successful generation of NGS data. In addition, the NGS dataanalysis protocol enables accurate STR allele call and repeat structure determinationat each locus. Therefore, this approach using the NGS system will be helpful to interpretand analysis the STR profiles from singe-source and even mixed samples inforensic investigation.
Preparation and Characterization of Monodisperse Polystyrene-Silica Nanocomposites
김은혜,김성수,김수연 한국고분자학회 2015 Macromolecular Research Vol.23 No.8
Polystyrene-silica nanocomposites were successfully prepared by UV-induced graft polymerization of styrene onto a silica surface. Composites were prepared by batch and continuous-circulation reactions, and results indicated that polystyrene was successfully grafted onto inorganic silica particles for both reactions. Scanning electron microscopy and thermogravimetry analyses of the composites indicated 45% volume increase and 37% polymer content after an optimal reaction time of 30 minutes, respectively, for the batch reaction. For the continuous-circulation reaction, the corresponding results were 63% volume increase and 42% polymer content after 140 min of reaction time. Fourier Transform-Raman spectroscopy analysis of composites showed the characteristic peaks of both silica and polystyrene thus confirming the successful graft-polymerization for both reactions. Disappearance of C-H and C=C ring stretching bands at 3070 and 1578 cm-1 in composites after their optimal reaction times for both reactions indicated a possible ring opening mechanism due to prolonged exposal to the UV light source. For the batch reaction, composites showed new vibration bands at 1052 and 812 cm-1 for asymmetric and symmetric Si-O-C stretching, respectively, indicating a covalent bond between the grafted polymer and silica surface. X-Ray photoelectron spectroscopy (XPS) analysis showed an increase in the elemental concentration of carbon from 5 to 46% for 30 minute composites in batch reaction. XPS analysis also indicated that the relative OH group content of the surface of the silica particles determines the effectiveness of the graft-polymerization of styrene.