김은숙 - 당선소감 / 추천의 말

김은숙,가영심,김양식,성기조 문예운동사 2007 문예운동 Vol.- No.93

24. 심한 충치로 인해 유치가 조기상실되었을때에 간격유지장치는?

김은숙 대한치과의사협회 1988 대한치과의사협회지 Vol.26 No.6

갑상선암의 표적 치료

김은숙 대한내분비학회 2010 Endocrinology and metabolism Vol.25 No.2

제 2 부 : 발표 1 ; 중국의 재정분권화에 관한 연구

김은숙 한국재정정책학회 2002 한국재정정책학회 정기학술대회 발표논문집 Vol.2002 No.-

문산에서

김은숙 한국외국어대학 학도호국단 1988 외대 Vol.23 No.1

그레이브스병에서 조절 T 세포에 의한 병원성 자가항원반응성 CD4+ T 세포의 억제효과

김은숙,정효원,최정일,남궁일성,홍순형,김영일 대한내분비학회 2007 Endocrinology and metabolism Vol.22 No.5

Background: Graves' disease (GD) is an organ-specific autoimmune disease that is characterized by lymphocyte infiltration of the thyroid, which finally leads to follicular destruction. The CD4+CD25+ regulatory T cells are important for maintaining peripheral tolerance to self-antigens and impaired activity can cause autoimmune diseases. CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily and expressed on activated T cells, is a candidate molecule for a co-stimulatory role in autoimmune thyroid disease. In this study, we aimed to assay the frequency of CD4+CD25+ T cells in GD patients and to investigate the role of CD137-mediated costimulation in CD4+CD25+ T cells.Methods: The frequencies of the CD4+CD25+ T cells in the peripheral blood (PB) of GD patients were determined by flow cytometric analysis. After the CD4+CD25+ T cells were isolated from PB mononuclear cells (PBMC) of the GD patients using immunomagnetic beads, the functional activity of the CD4+CD25+ T cells was characterized by use of a proliferation assay. mRNA expression of Foxp 3 in the CD4+CD25+ T cells of the GD patients was observed by real-time RT-PCR.Results: In this study, we found that GD patients had a low proportion of CD4+CD25+ T cells (mean ± SD; 1.47 ± 0.31%) in PBMC as compared with normal subjects. CD137-mediated costimulation increased the expression of CD25 and Foxp 3 in CD4+ T cells in GD patients as compared with normal subjects. Moreover, the CD137-mediated costimulation also induced the proliferation of CD4+CD25+ T cells in GD patients, and the expanded CD4+CD25+ T cells could suppress other CD4+CD25- T cells in a co-culture.Conclusion: These results suggest that the peripheral expansion of CD4+CD25+ T cells by CD137-mediated co-stimulation can suppress effector T cells and may be a potent therapy for Graves' disease. (J Kor Endocrine Soc 22:332~338, 2007) 연구배경: 면역조절 T 세포(CD4+CD25+ T 세포)는 면역 관용에 매우 중요한 역할을 하며 이들 세포의 기능에 이상이 생기면 자가면역질환이 생기게 된다. CD137 (4-1BB)은 활성화된 면역세포에서 발현되어 면역반응을 조절하는 대표적인 공동자극분자이다. 본 연구에서는 그레이브스병 환자의 말초혈액으로부터 CD4+CD25+ T 세포를 분리하여 CD137의 발현특성을 조사하고, CD137 매개의 공동자극을 통한 CD4+CD25+ T 세포 증식 유도가 병원성 자가항원 반응성 CD4+ T 세포의 기능을 억제할 수 있는지를 알아보고자 하였다.방법: 그레이브스병 환자의 말초혈액에서 CD4+CD25+ T 세포의 분포 및 CD137의 발현특성은 유세포 분석하였으며, CD137 자극에 의한 CD4+CD25+ T 세포의 증식 및 활성은 유세포 분석 및 proliferation assay를 통해 조사하였다. 또한 CD4+CD25+ T 세포에서의 FoxP3 유전자의 발현양상은 real-time PCR로 조사하였다.결과: 그레이브스병 환자의 단핵세포에서 CD4+CD25+ T cell의 발현은 낮은 것으로 나타났다. 그러나 4B4 처리에 의한 CD137 매개의 공동 자극은 그레이브스병 환자의 말초혈 내 CD4+CD25+ T cell의 증식을 유도하였으며, Foxp 3의 발현을 증가시켰다. 또한 CD137 공동자극에 의해 CD4+ CD25+ T 세포들의 CD4+CD25- T 세포의 증식억제 능력이 증가하였다.

초등학생의 전기 회로 이해에 따른 자연 교과서 전기 단원에 대한 분석과 제안

김은숙,정용재,심재규,장병기 한국과학교육학회 1999 한국과학교육학회지 Vol.19 No.4

The units about electricity in 5th and 6th science curriculum for elementary school was analyzed and the elementary students' understanding of related concepts was investigated. This study was to make connection between the research about alternative concepts and writing textbooks. It was found that students' response had following characteristics. They had strong belief that the + and - ends of battery should be connected. However this belief was not complete because students did not understand that the other circuit elements had to be connected properly. When the circuit involve more than one bulbs or batteries, they counted the number of batteries or bulbs only and paid little attention to the connection of them. In explaining circuits and the brightness of the bulbs in the circuits. students tried to explains the circuit using scientific terms but failed to used them properly. Although the concept of resistance was not dealt explicitly, student had reasonable qualitative understanding about resistance. According to response of students, several suggestions might be made for the curriculum. The structure and the circuit elements and the proper connection needs to be taught. Also more emphasis is necessary to pay attention to the connection in addition to the numbers of bulbs or batteries. Finally, it needs to be investigated carefully if it is better to introduce some qualitative model to explain the amount of current through bulb depending on the connection and the number of bulbs. It might also be better to introduce some terms, such as current and resistance with the model.

일회성 중등강도 유산소운동과 열 침수 순서가 비만 성인의 HSP72, 염증 반응 및 혈중지질에 미치는 영향

김은숙,김도현,윤성진 한국체육학회 2024 한국체육학회지 Vol.63 No.2

본 연구에서는 일회성 중등강도 유산소운동과 열 침수 순서가 비만 성인의 HSP72, 염증 반응 및 혈중 지질에 미치는 영향을 검증하고자 하였다. 연구대상자는 비만 성인 10명을 모집하여(남성 6명, 여성 4명), 중등강도 유산소운동 후 열 침수(moderate-intensity aerobic exercise after hot water immersion: EAH)와 열 침수 후 중등강도 유산소운동(hot water immersion after moderate-intensity aerobic exercise: HAE) 처치를 단일 집단 교차연구 설계로 진행하였다. 처치 중 RPE와체온을 측정하였으며(20분마다), 각 처치 전, 직후, 2시간 후, 6시간 후에 혈액샘플(HSP72,TNF-α, CK, hs-CRP, TG, TC, HDL-C, LDL-C)을 채취하여 분석하였다. 본 연구의 결과, RPE는 EAH에서 HAE보다 처치 60분 후, 직후 유의하게 낮게 나타났다. HSP72는 EAH와 HAE에서 모두 직후, 2시간 후 사전보다 유의하게 높게 나타났으며, 6시간 후에는 EAH에서만 유의하게높게 나타났다. TNF-α는 EAH와 HAE에서 모두 직후 사전보다 유의하게 높게 나타났으며, 2시간 후, 6시간 후에는 유의한 차이가 나타나지 않았다. CK는 EAH와 HAE에서 모두 직후 사전보다 유의하게 높게 나타났으며, EAH에서 HAE보다 2시간 후, 6시간 후 낮게 나타났다. TG는 EAH와 HAE에서 모두 직후, 2시간 후, 6시간 후에 사전보다 유의하게 낮게 나타났다. 따라서, 중등강도 유산소운동 후 열 침수가 비만 성인의 RPE, HSP72, 염증 반응 개선에 더 효과적이었으며, 중등강도 유산소운동 후열 침수와 열 침수 후 중등강도 유산소운동 처치 모두 혈중 지질에 긍정적인 변화를 유도할 수 있음을 시사한다. The purpose of this study was to investigate the effects of different sequences of acute moderate-intensity aerobic exercise and hot water immersion on HSP72, inflammatory response and blood lipids in obese adults. The participants were recruited from ten obese people (6 male and 4 female), and moderate-intensity aerobic exercise after hot water immersion (EAH) and hot water immersion after moderate-intensity aerobic exercise (HAE) treatments were conducted in a single-group crossover study design.RPE and body temperature were measured during treatment (every 20 minutes), and blood samples (HSP72, TNF-α, CK, hs-CRP, TG, TC, HDL-C, LDL-C) was collected and analyzed. As a result of this study, RPE was significantly lower in EAH than in HAE at 60 min during treatment, immediately after treatment. HSP72 was significantly higher immediately after treatment and after 2 hours in both EAH and HAE than pre, and was significantly higher only in EAH after 6 hours. TNF-α was significantly higher immediately after treatment in both EAH and HAE than pre, and there was no significant difference after 2 hours and 6 hours. CK was significantly higher immediately after treatment in both EAH and HAE than pre, and lower in EAH at after 2 hours and after 6 hours than in HAE. TG was significantly lower immediately after treatment, after 2 hours and 6 hours in both EAH and HAE than pre. Therefore, moderate-intensity aerobic exercise after hot water immersion was more effective in improving RPE, HSP72, and inflammatory responses in obese adults, and both moderate-intensity aerobic exercise after hot water immersion and hot water immersion after moderate-intensity aerobic exercise could induce positive changes in blood lipids.

치과위생사의 업무

김은숙 대한치과의사협회 2002 대한치과의사협회지 Vol.40 No.12

인지한 구강건강상태 , 구강건강 증진행위 및 일상생활 구강건강인지(DIDL)관계 분석

김은숙 대한구강보건학회 2000 大韓口腔保健學會誌 Vol.24 No.2

The purpose of this study was to determine the relationships among the perceived oral health, oral health promotion and dental impact of daily living in adults. The study was performed period between July 1 and December 1, 1998 in other to examine status perceived oral health, oral health promotion behavior and dental impact of daily living. The data consisted of random sample of 2,394 individuals whose ages(1,190 males and 1,194 females) were between 20 and 59. The data were analyzed using SAS version 6.04. The analytic methods for the study were t-test and correlation analysis. The results of the study were as follows; 1. There were significant positive relationships between sociodemographical factor and perceived oral health and between sociodemographical and oral health promotion behavior. 2. In case of positive respondents' of perceived oral health status, there was significant positive relationships of their dental impact of daily living. 3. The higher the oral health promotion was, the higher dental imnpact of daely living was. And the more the oral health promotion behavior was, the higher the perceived oral health status was, and the higher dental impact of daily living was.