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유방의 해면양 혈관종의 절제 후 보형물을 이용한 즉시 재건
김은기,이택종 대한성형외과학회 2006 Archives of Plastic Surgery Vol.33 No.5
Purpose: Hemangioma of the breast is an infrequent finding and usually encountered incidentally when checking for other disease. Most of hemangiomas of the breast are asymptomatic, not palpable perilobular type. Cavernous hemangioma of the breast is rare and only a few reports about this type of lesion are present. No example has been reported about reconstruction of the breast after resection of large cavernous hemangioma. Methods: We report here a case of immediate breast reconstruction using a Becker implant after subcutaneous mastectomy for a large cavernous hemangioma involving almost entire breast. Results: Symmetry is well maintained after 3 years without deformity or recurrence.Conclusion: The clinical prognosis of breast cavernous hemangioma is good after total excision and reconstruction.
Continuous Water Toxicity Monitoring Using Immobilized Photobacterium phosphoreum
김은기,서형준,Se Kwon Kim,Baek Seok Lee,이정건 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2
Water toxicity monitoring based on the continuous cultivation of Photobacterium phosphoreum is presented. Normally, after 10 days of operation, a dark variant, which emits no light, appears and dominates the population, resulting in a rapid decrease in bioluminescence. Therefore, to overcome this problem, a fluidized-bed reactor is used in which alginate-immobilized cells are grown and leaking cells are continuously released into the effluent. Experimental results revealed that the dominance of dark variants was suppressed inside the immobilized beads, thereby mitigating the rapid loss of bioluminescence. Plus, a high dilution rate (1.2 h-1) prevented the occurrence of other microbial contamination in the reactor. The concentration and bioluminescence of the released cells were sufficient to measure the water toxicity for more than 4 weeks.
횡복직근피판을 이용한 유방재건 시 복부절개선을 통한 반대측 유방 확대술
김은기,이택종 대한성형외과학회 2009 Archives of Plastic Surgery Vol.36 No.1
Purpose: Introduction of the mammary implant through the abdominal route has been well known since late 1960s, but the use of transabdominal route for contralateral breast augmentation in transverse rectus abdominis musculocutaneous(TRAM) flap breast reconstruction patients has not been reported in Korean literature. The authors report their experience with technical points as well as the selection of the appropriate patients. Methods: Simultaneous contralateral augmentation mammaplasty through transabdominal route was performed in 11 patients who underwent TRAM breast reconstruction from August 2003 to May 2008 with a mean follow up of 27 months. The pocket was created under direct vision: 3 subglandular, 7 subpectoral, and 1 dual plane was dissected. Eight saline and 3 silicone gel implants were used with an average volume of 165 cc. Results: There were no complications such as infection, hematoma, implant displacement, and capsular contracture. The results were well maintained throughout the follow-up period. Conclusion: Transabdominal route could be recommended in selected patients for contralateral augmentation in TRAM breast reconstruction.
백서 복직근판의 허혈-재관류 손상에 대한 Erythropoietin의 영향
김은기,홍준표 대한성형외과학회 2006 Archives of Plastic Surgery Vol.33 No.5
Purpose: Erythropoietin is traditionally known to regulate erythropoiesis, but recently its protective effect against ischemia-reperfusion injury has been studied mainly in cardiovascular and neuronal systems. This study was planned to investigate the effects of recombinant human erythropoietin on ischemia-reperfusion injury in rat TRAM flap model. Methods: Superiorly based TRAM flap was elevated and ischemic insult was given for four hours. Thirty minutes before reperfusion, single dose recombinant human Erythropoietin(5000IU/kg) was injected via intraperitoneal route in the treatment group. At 24 hours postoperatively, systemic neutrophil count, tissue myeloperoxidase activity, malonyldialdehyde amount, nitric oxide content, tissue water content and histologic finding of inflammation was evaluated. On 10 days postoperatively, flap survival rate, angiogenesis and change in hematocrit level was evaluated. Results: Tissue nitric oxide level was significantly higher and myeloperoxidase activity was significantly lower in the treatment group 24 hours after reperfusion. Tissue water content was significantly lower in the treatment group. Perivascular neutrophil infiltration and intravascular adhesion was marked in the control group. Mean flap survival after ten days was 69% in the treatment group, and 47% in the control group, demonstrating a significant difference. Neovascularization in the treatment group also outnumbered the control group. No significant hematocrit rise was noted ten days after erythropoietin administration. Conclusion: Recombinant human Erythropoietin improved flap survival in ischemia-reperfusion injured rat TRAM flaps, at least partially owing to suppressed inflammation, increased nitric oxide, and enhanced angiogenesis.