CVD 반응기내 기체유동의 균일성 향상에 관한 수치적 연구

김유재(Y-J. Kim),김윤제(Youn J. Kim) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.8

감나무 잎으로 부터 분리한 흰색 결정체의 구조 분석 및 심장에 대한 작용

김창호,차영덕,이종화,김유재,김인정,김종배,조병헌,김천숙 순천향대학교 1992 논문집 Vol.14 No.4

The study was undertaken to search for a new agent in natural crude plants which might ameliorate cardiovascular diseases. Extracts of the leaves of Diospyros kaki Thunb have been used as folk remedies of several diseases, including hypertension and disorders of peripheral vascular system. But any study on the pharmacological action of this plant has not yet been carried out and its active ingredients have not been identified. In this study, we isolated white crystal from the chloroform-soluble fraction of this plant. This white crystal was indentified as dimethyl-p-phthalic acid (DPP, ??). We examined the pharmacological actions of DPP: effects on the rat electrocardiogram, the isolated rat heart and the contractile force of right ventricular muscle induced by electrical stimulation. The observed results are as follows: 1) DPP increased the contractile force of isolated rat heart. 2) DPP increased the contractile-force of the right ventricular muscle induced by electrical stimulation. 3) DPP increased heart rate and shortened the QT interval in the rat electrocardiogram.

사염화탄소(CCl₄)에 의한 간손상 흰쥐의 Trithioformaldehyde의 방어효과(Ⅰ)

김영석,김종배,김천숙,권준택,최규홍,이종화,조병헌,차영덕,김유재 순천향대학교 1989 논문집 Vol.12 No.3

Trithioformaldehyde(TTFA) is a cyclic aliphatic compound containing three sulfur atoms. TTFA was synthetized from formalin and hydrogen sulfide in our Lab.. Pharmacological effects of TTFA were clarified in our Lab.: antifungal effects, and radiation protective effect in mice. In this experiment, the protective effect of TTFA on CCl₄ induced hepatotoxicity in rats was evaluated histopathologically. Groups consist of net control, administered with CCl₄ only and TTFA pretreatment. CCl₄(1ml/kg) was given i.p. as a 50%(V / V) solution in olive oil. TTFA(300mg/kg) suspended in 5% arabia gum was given i.p. 24hrs and 20min before CCl₄ administration. The rats that received CCl₄ were sacrificed 24hrs later. In pathological finding, massive fatty change with necrosis in the centrilobular area is found in CCl₄ only treated group. In TTFA pretreated group, a slightly necrotic change in the midzone is noted. Remarkable reductions in hepatic glycogen were observed in CCl₄ only administration. TTFA prevented them from disappearing. These results show that TTFA has a significantly protective effects aganinst CCl₄ induced hepatotoxicity.

흰쥐 간장에서 허혈-재관류에 의한 간장해 모델의 연구(1)

권준택,이종화,조병현,양훈모,박종안,김유재,김종배,차영덕,김창호 최신의학사 1992 最新醫學 Vol.35 No.12

흰쥐 적출 심근의 수축 현상에 대한 cyclobuxine과 칼슘 효현제인 Bay K 8644의 상호작용

박영현,김유재,김종배,차영덕,이종화,조병헌,권준택,김창호 순천향대학교 1992 논문집 Vol.15 No.2

Cyclobuxine, the major alkaloid of Buxus microphylla var. koreana Nakai, is a steroidal alkaloid recognized to contain a cyclopropane ring and has a substitution pattern at C-4 and C-14. In our previous studies, cyclobuxine blocked potassium-activated calcium channels(voltage-sensitive calcium channels) in ileal smooth muscle. Cyclobuxine also exerted negative chronotropic and inotropic effects in the anesthetized rat and the isolated rat heart. In this study, antagonism between either the dihydropyridine calcium agonist, Bay K 8644, and high external Ca++ or nifedipine, verapamil, diltiazem, and cyclobuxine was investigated in the isolated ventricular muscle from rats. Bay K 8644(?? M) produced a slowly developing increase in developed tension. Cyclobuxine and the calcium antagonists decreased dose-dependently developed tension. Bay K 8644(??M) produced a rightward parallel shift of the concentration-response curves for the negative inotropic effects of nifedipine(??). Bay K 8644 produced only a slight rightward shift of the concentration-response curves for the negative inotropic effects of diltiazem(??) and cyclobuxine (??). These results indicate that Bay K 8644 antagonizes the negative inotropic effect of nifedipine by competing with the same site closely associated with the calcium channel. In contrast, Bay K 8644 antagonizes noncompetitively the negative inotropic effects of cyclobuxine, verapamil and diltiazem in the isolated ventricular muscle.

Majarine 및 그 유도체인 Trihydroxymajarine과 Tetrahydromajarine의 토끼 적출장관에 대한 작용

이종화,박영현,조병현,김유재,김종배,김천숙,차영덕,김영석 순천향대학교 1986 논문집 Vol.9 No.3

Majarine is the isoquinoline alkaloid with quaternary ammonium isolated from the root of Berberis koreana Palibin distributd only in Korea. We had clarified many pharmacological effects of majarine: the bacteriostatic effect in vitro; the potentiation of hypnotic action of alcohol: hypotensive effect in rats and hypothermic effect in mice. In this experiments, trihydroxymajarine (THM) was obtained from majarine by a aluminium chloride-thiol reagent and tetrahydromajarine(TeHM) was obtained from jajarine by reduction. We examined the effects of majarine, trihydroxymajarine and tetrahydromajarine on isolated rabbit-small intestine. Majarine decreased the motility of isolated rabbit-small intestine. The increase in contraction tension induced by acetylcholine(0.55×10(-6)M) was significantly inhibited by majarine(4.32×10(-⁴)M), but majarine(4.32×10(-⁴)M) did not block significantly the contractile effect elicited by barium chloride(4.1×10(-⁴)M). Tetrahydromajarine did not affect on isolated rabbit -small intestine. Trihydroxymajarine exerted an increase in contraction tension. The increase in contraction tension induced by trihydroxymajarine (2.42×10(-⁴)M) was blocked by papaverine(2.9×10(-5)M), but not by atropine(0.86×10(-7)M)

Cyclobuxine D의 토끼 적출 장관에 대한 작용

이종화,박영현,조병헌,김종배,김정목,김유재,김천숙,차영덕,김영석 최신의학사 1988 最新醫學 Vol.31 No.1

Majarine의 중추신경계에 대한 작용(Ⅲ) : Modification of Tetrahydromajarine-induced Hypothermia by Drugs Affecting Brain Monoamine Functions in Mice Tetrahydromajarine의 마우스 체온하강 작용에 대한 뇌 Monoamine에 미치는 약물들의 작용

이종화,조병헌,김종배,김유재,김천숙,차영덕,김영석,박영현 순천향대학교 1986 논문집 Vol.9 No.2

한국 특산 매자나무(Berberis koreana Palibin)의 뿌리에서 분리한 majarine은 4급 ammonium의 isoquinoline 알칼로이드로서 본교실에서는 중추신경계에 대한 약리작용을 검토하고 있다. Majarine을 보다 지용성이고 뇌혈관 장벽(Blood-brain barrier)을 투과하기 쉬운 3급 amine인 tetrahydromajarine(THM) 으로 환원시켰다. Tetrahydromajarine을 마우스 복겅내 투여하여 직장온도 변화와 뇌 monoamine에 영향을 주는 약물들은 전처치하여 약물상호작용을 관찰하여 다음과 같은 성적을 얻었다. 그 성적을 통해서 tetrehydromajarine의 체온하강 작용에 대한 뇌 monoamine의 작용기전을 밝히고져 하였다. Tetrahydromajarine(0.5-50 mg/kg)은 마우스에 있어서 용량의존적으로 현저한 체온감소를 나타내었으나, 0.5mg/kg 투여시 체온하강의 유의성은 없었다. THM의 체온하강은 5-hydroxytryptamine(5-HT: serotonin) antagonist인 cyproheptadine인 5-HT depletor의 p-chlorophenylalanine에 의해 유의성있게 억제되었다. Dopamine(DA) antagonist인 haloperidol에 의해 THM의 체온하강이 억제되었으나, 50 mg/kg 투여시 완전하게 억제되지 않앗다. 5-HT와 noradrenaline(NE)의 reuptake inhibitor인 imipramine과 DA와 NE의 합성요소인 tyrosine hydroxylase inhibitor인 a-methyl-p-tyrosine의 전처치시 tetrahydromajarine의 체온하강을 완전하게 억제시키지 않았으나, THM 50 mg/kg 투여시 유의성을 나타내었다. Reserpine antagonism test에서 tetrahydromajarine(10-50 mg/kg) 전처치후 reserpine을 마우스 정맥내 투여시 hypothermia reversal 없이 용량 의존적인 체온감소를 나타내었다. 이러한 결과로 보아 tetrahydromajarine의 체온하강은 뇌 monoamine인 5-HT와 직접적인 관련성이 있고, dopamine도 연관된다고 사려된다. Majarine is the isoquinoline alkaloid with quaternary ammonium isolated from the root of Berberis koreana Palibin distributed only in Korea. Authors had investigated its pharmacological action on the central nervous system. Tetrahydromajarine(THM) with tertiary amine obtained by reduction of majarine is more lipid soluble and will be expected to penetrate into the blood-brain barrier. We report the results of a series of experiments to investigate further the role played by dopaminergic and serotonergic mechani는 in the action of tetrahydromajarine on body temperature in the mouse. Hypothermia was dose-dependent at a does of 0.5, 10 and 50mg/kg of tetrahydromajarine, but not significant at a does of 0.5mg/kg in mice. By pretreatment with 5-hydroxytryptamine antagonist, cyproheptadine and 5-HT depletor, p-chlorophenylalanine, the hypothermic effect of THM was blocked significantly or completely. Pretreatment with dopamine antagonist, haloperidol, completely blocked the hypothermia normally induced by tetrahydromajarine, but about 50% of the hypothermia induced by 50 mg/kg. Pretreatment with reuptake antagonist of 5-HT and noradrealine, imipramine and tyrosine hydroxylase(DA and NE synthesizer) inhibitor, a-methyl-p-tyrosine, did not completely blocked the tetrahydromajarine-induced hypothermia, but significantly at a does of 50 mg/kg. Reserpine-induced hypothermia and its reversal by tetrahydromajarine(10-50 mg/kg) was not significant and it produced a dose-dependent hypothermia. Regarding the mediation of the hypothermic effect of tetrahydromajarine in the brain monoamine functions, our results support a significant role of direct related 5-HT and DA.

연령적 추이에 대한 폐기능력에 관한 연구

심동원,김유재 순천향대학교 1991 논문집 Vol.14 No.2

In order to evaluate the age-related changes of pulmonary fuction, the vital capacities, tidal volumes, expiratory reserve volumn and respiratory rates were meansured in 1592 normal men and women aged between 10 to 49 years. The volunteers were composed of 355 boys and 402 girls aged between 10 and 18 years, 490 adults men and 289 adult women aged between 19 and 29 years, and middle age men in their thirties and fourties. The degrees of concomitant rise in lung volumn in accordance with the growth of physical body feature were measered in accordance with the growth of physical body feature were measered in boys and girls and reflections of body status in lung volumns were analyzed for the young adults. The age dependency of the inverse relation between lung volumn and body weight was also cheked of the middle aged men. Results are as follow : 1. During the age of 10 to 18, both the vital capacity and tidal voume increased with the progress of age : 2. The vital capacities and tidal volumes of young adults aged between 19 and 29 years remained constant. 3. The vital capacities and tidal volumes of middle aged men are found to be decrease with the progress of age. However, the decrease of vital capacities are more extensive than volumes. 4. In growth generation(10-18), adolescence(19-29), middle mature age(30-49), by the distinction of sex, the relation between vital capacity, tidal volume and body weight is very large(r >0.57), is indicative of simple regression curre.