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      • KCI등재후보
      • SCOPUSKCI등재

        Methanol을 이용한 개 정액 동결시 융해후 양호한 활력 및 생존율을 나타내는 정액 처리 조건

        김용준,박영재,김병진,유일정,Kim Yong-jun,Park Young-jae,Kim Byeong-jin,Yu Il-jeoung 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2

        This experiment was carried out to investigate the renditions to maintain good post-thaw motility and viability of canine spermatozoa when the semen was frozen using methanol. The semen from two male dogs which had been proven to be fertile in the previous one year was treated with different compositions of semen diluent and was frozen at different freezing temperatures, When canine semen was frozen at-2$0^{\circ}C$, -6$0^{\circ}C$, or -8$0^{\circ}C$, the spermatozoa frozen and stored at -2$0^{\circ}C$ showed very low post-thaw motility and viability from day 2 to 7 and showed no viability since day 15 after freezing. The spermatozoa frozen and stored at -6$0^{\circ}C$ or -8$0^{\circ}C$ showed higher post-thaw motility and viability on day 2, 1, 15 and 30 after freezing than that frozen and stored at-2$0^{\circ}C$(p<0.01), with no difference between two groups. Among different composition groups of the semen diluents of control(tris + egg yolk + glycerol), egg yolk-free, 히ycerol-free, and tris-free, Prior to freezing, the egg yolk-free diluent showed significantly love. motility and viability than the other diluents(p<0.05). On each thawing day (from day 2 to 15 after freezing), control group showed considerably higher motility and viability than the other groups(p<0.01). The canine spermatozoa frozen and stored at -6$0^{\circ}C$ and -8$0^{\circ}C$ showed gradual decrease of motility from day 2 to 30 after freezing and the spermatozoa of these two groups thawed on day 30 showed considerably love. motility than those thawed on day 2 after freezing, respectively(p<0.01). These results indicate that the freezing temperature of either -6$0^{\circ}C$ or -8$0^{\circ}C$ can be applicable to the freezing method using methanol and also all of the components of the semen diluent including cryoprotectant, buffer and cold-shock buffer are very important to maintain motility and viability of canine spermatozoa in the freezing and thawing procedure.

      • SCOPUSKCI등재

        암반발파 및 중장비 사용후 발생한 돼지유산 및 불임증에 대한 원인 조사

        김용준,조문희,조정곤,김명순 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.2

        These investigations were performed to determine the causes of the reproductive disorders of the sows in a pig farm which was located at the distance 36m away from the detonation spot. The reproductive problems of the sows such as abortion and infertility occurred after consecutive detonation and use of heavy equipments to build 6 piers of the bridge for the expressway between Jinju and Taeieon during the period from July 1997 to October 1998. 1. The management condition of the farm, microorganism in the feed, inbreeding, hematological conditions, and viral agent causing abortion were not proved as the factors of the occurrence of abortion and infertility in the pig farm. 2. Theoretical concussion values according to the average amount of explosive per detonation were 1.16 kine for minimum amount (3.6 kg) and 3.45 kine for maximum amount(12.5 kg) of explosive, and the theoretical sound values were accordingly 92.29 decibel for minimum amount and 101.76 decibel for maximum amount of explosive, respectively, in the case of 36m distance between the detonation spot and pig farm. The frequency of detonation in a day was 7 times with interval of 15 minutes. 3. The occurence of infertility in the farm was 57.3% during the period of construction from July 1997 to August 1998. 4. In comparison of body weight of piglets between the damaged and not damaged farms, growth of piglets at the damaged farm was shown to be delayed over 30 days than those of not-damaged farm. In conclusion, abortion of 40 sows, delivery of whole still births of 22 sows, and infertility of 122 sows in the pig farm were assumed to be caused by the concussion or sound stress after consecutive detonation and use of heavy equipments.

      • SCOPUSKCI등재

        개의 발정기에 발생한 자궁혈증의 1례

        김용준,오홍근,김남수,조정곤,지동범 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.2

        A four-year old bitch which showed occasional hemomorragic exudate from the vulva for two weeks after the previous estrus 7 months ago was ovariohysterectomied. About 200m1 of sanguineous fluid was flowed out from the uterus and some scattered blood clots were found in both uterine horns. Hematometra of the bitch which was presumed to occur during the proestrus two weeks ago was thought to progress into early pyometra at the time of diagnosis. To diagnose hematometra, vaginal examination using vaginal speculum or vaginoscope and hematological examination were performed. Radiography and ultrasonography were also proved to be of use to diagnose hematometra. Treatment for hematometra was considered to be the same as for the case of pyometra.

      • SCOPUSKCI등재

        말에서 조기 임신진단을 위한 동음파영상 이용 방법에 관한 연구

        김용준,서세현,김명철,최종복 한국임상수의학회 1998 한국임상수의학회지 Vol.15 No.1

        To investigate the estrual changes of ovaries, appropriate time of mating, diagnosis of early pregnancy after mating, and diagnosis of twin fetuses, ultrasonography was performed in 61 thorough-bred mares (5-12 years old) which had been raised in Chrju island. The followings are the results obtained: 1. The average size of a follicle of mares was $3.2{\times} 3.8cm$ at the beginning of estrus and $4.2{\times} 3.5 cm$ from the middle of estrus to ovulations respectively, and the average size of a follicle and a corpus luteum was $1.8{\times} 1.4 cm and 3.1{\times}2.3 cm$ in the middle of diestrus, respectively. 2. In the result of pregnancy according to mating time after ultrasonographic examination of follicle size, the mares having a follicle of 4.6-5.0 cm (diameter) were mated on the day of ultrasonographic examination or the next day rind all were conceived (3 mares) and another mares having a follicle of 4.1- 4.5cm (diameter) on the 1st or 2nd day after examination and all were conceived (4 mares). 3. By ultrasonographyi early pregnancy in the mares was diagnosed from 12 days of pregnancy and the equine fetus was directly detectable from 23 days of pregnancy, whereas the heartbeat of a fetus was detectable from 28 days of pregnancy. 4. Diagnosis of earl pregnancy of twin was possible from 16 days of pregnancy. These result indicated that ultrasonography is applicable in mares to diagnose the estrual changes of ovaries and the time of ovulation or mating, and to diagnose early pregnancy from 12 days of pregnancy and early pregnancy of twin fetuses.

      • SCOPUSKCI등재

        개 정맥의 동결 및 융해후 정자의 생존성 및 수정능획득 판정을 위한 HOS test 및 CTC test

        김용준,지동범,오홍근 한국임상수의학회 2000 한국임상수의학회지 Vol.17 No.2

        Evaluation of viability and capacitation of canine sperm is of great importance to deter- mine good condition for freezing canine semen and consequently to improve conception rate by arti-ficial insemination. Semen were collected from nine male dots which had been proved to be fertile in the post and the semen were treaded for freezing procedure. Semen were thawed at 37$^{\circ}C$ for 30 seconds. In this study, hypoosmotic swelling(HOS) test and chlortetracycline(CTC) test were per- formed to evaluate post-thaw viability and capacitated status of sperm, respectively. In HOS test far canine sperm, the highest percentage of curled sperm was shown at 60 mOsm. In HOS test for canine semen, there were considerably significant correlation between HOS values and sperm motil- ity(r=0.9064, p<0.01) and converse correlation between HOS values and sperm abnormality(r=- 0.6905, p<0.05). The sperm viability and HOS-values for chilled extended semen were significantly decreased from 0 to 72 hours during storage at 5$^{\circ}C$ (p<0.05). Of the media added to canine semen after thawing, the most capacitated sperm were shown in CCM(p<0.01), and then This Fructose Cit- rate(TFC) medium with calcium from 3 hours after incubation with media. It was concluded that HOS test is of great value to determine the viability and motility of canine sperm, whereas CTC test is usable to determine the capacitated status. Consequently, both tests were thought to be useful as the additional tests to standard semen analysis.

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