Agrobacterium을 이용한 형질전환 상추의 세포 현탁배양으로부터 hGM-CSF의 생산

김영숙,김미영,권태호,양문식,Kim, Young-Sook,Kim, Mi-Young,Kwon, Tae-Ho,Yang, Moon-Sik 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1

hGM-CSF가 식물세포 현탁 배양을 통하여 생산이 가능한지를 조사하기 위하여 hGM-CSF를 포함하고 있는 A. tumerfaciens LBA4404를 가지고 상추에 형질전환시켰다. 형질전환된 상추로부터 캘러스를 유도하여 캘러스를 이용한 세포배양체계를 확립하였다. PCR과 Southern blot analysis 결과 상추에 hGM-CSF 유전자가 도입된 것을 확인하였으며, Northern blot analysis 결과 상추식물체에 hGM-CSF 유전자가 발현됨을 확인하였다. 현탁 배양 세포로부터 분비된 hGM-CSF를 ELISA를 이용하여 측정한 결과 149.0 $\mu\textrm{g}$/L가 생산됨 을 확인하였다 이러한 결과는 상추의 현탁 배양 세포가 hGM-CSF와 같은 치료용 단백질의 생산 숙주로 이용될 수 있음을 보여주었다. Lettuce (Lactuca sativa) was transformed with Agrobacterium tumefacience LBA4404 containing human granulocyte macrophage colony stimulating factor (hGM-CSF) gene to produce in cell suspension cultures. Cell suspension culture was established using callus from transgenic lettuce plant. Integration of hGM-CSF gene into plant chromosome was confirmed through genomic PCR and Southern blot analysis. In addition, Northern blot analysis indicated the expression of the introduced hGM-CSF gene in transformed lettuce. The recombinant hGM-CSF was expressed in transgenic cell cultures derived from transgenic plants as a yield of about 149.0 $\mu\textrm{g}$/L in culture filtrate, which was determined by ELISA. These results demonstrated that transformed lettuce cell suspension cultures could be used as a production system of therapeutic proteins such as hGM-CSF.

철-크롬 산화환원흐름전지에서 Nafion막의 철-크롬 Crossover

김영숙,오소형,김은비,김다영,김성지,추천호,박권필,Kim, Young-Sook,Oh, So-Hyeong,Kim, Eunbi,Kim, Dayoung,Kim, Seongji,Chu, Cheun-Ho,Park, Kwonpil 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.1

산화환원흐름전지(Redox Flow Battery, RFB)는 대용량 에너지 저장장치로 바나듐 산화환원흐름전지가 대표적인 RFB인데, VRFB는 고가인 점이 문제다. 철-크롬RFB는 저가의 활물질을 사용해 경제적인 점이 장점인데, 성능이 낮은 점이 해결해야할 과제다. 낮은 성능의 한 원인이 활물질의 크로스오버인데, 본 연구에서 철과 크롬 이온의 Nafion 막 크로스오버 및 Nafion 막의 안정성에 대해 실험하였다. 철과 크롬이온의 Nafion 막 투과도는 각각 $5.5{\times}10^{-5}$, $6.0{\times}10^{-5}cm^2/min$ 이었다. Nafion 막에서 바나듐 이온의 투과도 $2.9{\times}10^{-6}cm^2/min$ 보다 18.9~20.7배 높아 철과 크롬 이온의 Nafion 막 크로스오버가 성능 저하의 한 원인임을 보였다. 온도 증가에 따라 크로스오버가 급증(활성화 에너지 38.8 kJ/mol)하므로 낮은 온도에서 구동하는 것이 크로스오버에 의한 성능감소를 저하시키는 방법임을 나타냈다. Nafion막은 3M HCl용액에서 비교적 안정적이었다. The redox flow battery (RFB) is a large-capacity energy storage equipment, and the vanadium redox flow cell is a typical RFB, but VRFB is expensive. Iron-chrome RFBs are economical because they use low-cost active materials, but their low performance is a urgent problem. In this study, the crossover of iron and chromium ion through Nafion membrane and the stability of Nafion membrane in HCl solution were investigated. The permeability of iron and chrome ion through Nafion were $5.5{\times}10^{-5}$ and $6.0{\times}10^{-5}cm^2/min$, respectively, which was 18.9~20.7 times higher than that of vanadium ion ($2.9{\times}10^{-6}cm^2/min$). The crossover of iron and chromium ions were shown to be a cause of performance decrease in Iron-chrome RFB. As the temperature increases, the crossover increases rapidly (activation energy 38.8 kJ/ mol), indicating that operation at low temperature is a methode to reduce the performance loss due to crossover. Nafion membranes were relatively stable in 3 M HCl solution.

詠史詩의 신라멸망기 '鮑石亭宴' 史話 수용 양상과 의미

김영숙(Kim, Young-sook) 대동한문학회 2008 大東漢文學 Vol.29 No.-

영사시는 역사를 읊은 시로서 중국에서부터 오랜 전통을 지니고 있으며 한국에서도 신라시대부터 영사시가 있었다. ‘포석정연’은 신라 멸망기인 경애왕 4년(927)에 경애왕이 왕비, 궁녀, 친척들과 포석정에서 잔치한 것이다. 이것은 경애왕이 왕비, 궁녀들과 잔치를 즐기던 중 갑자기 견훤이 군사를 이끌고 침입하여 경애왕을 자살하게 하고 경순왕을 추대한 역사적 대사건이다. 이 사실은 『삼국사기』, 『삼국유사』, 『고려사』, 『신증동국여지승람』, 『동국통감』, 『동사강목』에 실려 있는데, 고려와 신라시대의 여러 문인들이 이를 수용하여 영사시를 지었다. 그 가운데서도 이인로의 「포석정」, 김종직의 「포석정」, 김시습의 「포석정」, 조위의 「포석정」, 서거정의 「포석정」, 이익의 「포석정」, 이광사의 「포석정」, 이영익의 「포석정」, 이학규의 「포석정」, 이복휴의 「포석정」은 대표적이고 중요한 작품이다. 이 작품들의 공통성은 경애왕이 왕비와 궁녀를 거느리고 포석정에서 잔치를 벌이다가 견훤의 침입으로 화를 당한 내용을 수용하여 시를 지은 것이다. 차이점은 전체적인 공통성 위에 작가마다 개인의 정회를 읊어 서로 다른 시적 형상을 한 것이다. 조선 전기 문인들의 작품은 작가들이 직접 포석정을 찾아가서 보고 느낀 정회를 읊었다. 이 작품들은 전해지고 있는 역사를 수용하여 당시를 회고한 것으로, 경애왕의 실정을 비판하여 후인들에게 경계심을 주고 있다. 조선 후기 문인들의 작품은 영사악부이다. 이것은 기록으로 전하는 역사를 수용하여 장단구 형식으로 지은 작품이다. 이것은 역사에 대한 작가의 의논을 중점적으로 형상하여 경애왕의 실정을 비판하여 후인들에게 경계심을 주고 있다. 영사시를 지은 작가들은 역사의식이 강하며 역사에 대한 비판정신도 높다. 영사시는 작가의 당대는 물론 후대에까지 감계를 준다. ‘Yeongsasi(詠史詩)’ is the poem that the history is recited, has the long tradition from China and there were Yeonsasis in Silla. ‘Poseokjeongyeon’ is the banquet that ‘King Gyungae(景哀王)’ hold at the ‘PoseokJeong(鮑石亭)’ with the Queen, the court ladies, the relatives in the Silla's fall, 927(the fourth year of King Kyungae). This is the historical big event that ‘Gyeonhwon(甄萱)’ had ‘King Gyeongsun’ as the king on get ‘King Gyungae’ suicide after he suddenly invaded with the soldiers while ‘King Gyungae’ enjoyed the banquet with the Queen and the court ladies. The 『Samguksagi』 has this fact. The several literary men made these ‘Yeongsasi’ on accepting this. The 「Poseokjeong」 of Lee, In-ro(李仁老), the 「Poseokjeong」 of Kim, Jong-jik(金宗直), the 「Poseokjeong」 of Kim, si-seup(金時習), the 「Poseokjeong」 of Jo, wi(曺偉), the 「Poseokjeongkamhwoi」 of Seo, Geo-jeong(徐居正), the 「Poseokjeong」 of Lee, ik(李瀷), the 「Poseokjeong」 of Lee, Kwang-sa(李匡師), the 「Poseokjeong」 of Lee, Young-ik(李令翊), the 「Poseokjeong」 of Lee, Hak-kyu(李學逵) and the 「Poseokyeon」 of Lee, Bok-hyu(李福休) are the typical and important works. The common point of these works is the poem accepting the content that King Gyungae’ meet the disaster by Gyeonhwon's invasion while he holds the banquet at the ‘PoseokJeong’ with the Queen, the court ladies. The different point is that each writers made the different poetic images with their own dear memories. The works of the literary men early in Joseon include the dear memories that they directly visit ‘PoseokJeong’ and saw and felt. These works looked back that time on accepting the history that has come down and warn the future generations by criticizing the King Gyungae's actual circumstance. The works of the literary men late in Joseon are the ‘Yeongsaakbu(詠史樂府)’. They are the ones that were made by the long and short phrases on accepting the history that has come down by the records. They concentrate on forming the consultation of the writers about the history and warn the future generations by criticizing the King Gyungae's actual circumstance. The writers who made the Yeongsasis have the strong historical consciousness and high critical mind about the history.

High Levels of Protein Carboxyl Methyltransferase in Abnormal Tissues and Changes of its Isoenzymes

김영숙,전길자,Kim, Young-Sook,Jhon, Gil-Ja 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

정상 혈액, 위, 유방조직 및 당뇨병 혈액, 위암, 유방암 조직에서 protein carboxyl methyltransferase의 활성도를 비교 측정하였고, 각각에서 그 isoenzyme의 변화를 관찰하였다. 그 결과 protein carboxyl methyltransferase의 활성도가 당뇨병 혈액, 위암, 유방암 조직에서 그 정상과 비교해서 약 2-5배 증가됨을 볼 수 있었고 isoenzyme의 분포가 정상과 비정상에서 다르게 나타났다. 이러한 결과로 protein carboxyl methyltransferase의 활성도 측정 및 그 isoenzyme의 변화 양상이 질병을 진단하는 데 이용될 가능성을 제시할 수 있다. The levels of protein carboxyl methyltransferase, an enzyme that methylates free carboxyl groups of altered protein and its isoenzymes were determined in normal and abnormal tissues (normal blood, diabetic blood, normal stomach and its tumor, normal breast and its tumor). Protein carboxyl methyltransferase activities were higher in the diabetic blood and the tumors than in their normal tissues. The distributions of protein carboxyl methyltransferase isoenzymes in normal tissues were different from in abnormal tissues. These results suggest the measurements of PCM activity and its isoenzymes offer a possible clinical diagnosis.

정신 분열증과 간호 - 원인$\cdot$증상$\cdot$간호

김영숙,Kim Young Sook 대한간호협회 1967 대한간호 Vol.6 No.6

노년기 여성의 상반신 체형의 유형화

김영숙,손희순 ( Young Sook Kim,Hee Soon Sohn ) 대한가정학회 1996 Family and Environment Research Vol.34 No.3

경남지역 일부 산업간호사의 업무수행에 관한 연구

김영숙,손혜숙,강정학,이창희,이채언,Kim, Young-Sook,Shon, Hae-Sook,Kang, Jeong-Hak,Lee, Chang-Hee,Lee, Chae-Un 대한예방의학회 1995 Journal of Preventive Medicine and Public Health Vol.28 No.3

Authors purposed to observe activities of occupational health nurses and it's related factors, and to suggest the way that induct better occupational health nurses' activities with questionaire to 87 occupational health nurses who individually work as health manager in the plant. The questionaire included type of plant and number of workers, general characteristics, work conditions, activities, etc. Major findings are as follows. 1. 82.8% of occupational health nurses were third decade. 93.1% graduated junior college or college. And 82.8% were not married. 2. General work conditions: 40.2% were belonged to safety-health section, 98.85% were mere clerks. 60.9% worked less than weekly 44 hours, and an annual salary of 50.6 % was between 10 million and 14 million won. 3. Work condition related to health manager work: there was separated health care room in 94.3%, working period as health manager(occupational health nurse) was less than 5 years in 70.1%, 49.4% had the out-of-health manager work. In 87.4%, occupational physician was appointed, only 6.9% of them were full time, 52.9% of them worked little in the plants. The problems related to workers' health were discussed with industrial nurses in 88.5%. 4. Attitude for their work: 88.5% were thought that their work is important for workers' health care, 57.5% satisfied to work as health manager. In 51.7%, motive to being industrial nurse were the appropriate aptitude. 5. Activities: General medical care in 100% were carried out, in 97.7% works related to general health examination, in 100% works related to special health examination were carried. But works related to use of protective apparatus were carried out in 20.8%. 6. Factors related to level of activities: In cases who solved the health related problems by themselves, the level of activites was significantly higher than in others. In cases there were full time occupational physician, the level of activities was significantly lower. 7. Occupational health nurse's needs: 100% wanted regular education, 89.7% wanted the qualifying examination. As the results, author suggests that the right of self-control is given to occupational health nurses and the work of occupational physician is clearly defined for the induction of the better activities of occupational health nurses.

황기(黃耆)의 간기능 보호 성분

김영숙,경종수,박기현,백남인,Kim, Young-Sook,Kyung, Jong-Soo,Park, Ki-Hyun,Baek, Nam-In 한국생약학회 1996 생약학회지 Vol.27 No.2

The components were isolated from the root of Astragalus membranaceus and their structures were characterized as 3,4-methylenedioxypyrrolealdehyde, $7-O-{\beta}-D-glucopyranosyl$ 7, 3'-dihydroxy-4'-methoxy isoflavone and a naphthalene derivative on the basis of spectral and physical methods. $7-O-{\beta}-D-glucopyranosyl$ 7, 3'-dihydroxy-4'-methoxy isoflavone and the naphthalene compound showed protective effect on $CC_{l4}-induced$ cytotoxicity in primary cultured rat hepatocytes.

백반증 환자에서 PUVA 치료후 나타난 부작용에 대한 임상적 고찰

김영숙,강형철 ( Young Sook Kim,Hyung Chul Kang ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.4

Background: Oral of topical psoralen photochemotherapy is the most popular arid efficacious treatment, available for repigmentation of vitiliginous patches. However this therapy is often used for prolonged periods and various types of pigmentary changes can be observed and sometimes, may be carcinogenic. The safety of PUVA therapy has been an issue of debate. Objective .' The purpose of the study was to describe in more detail the complications of PUVA treatment and to investigate a possible relation with multiple factors. Patients and Method: We studied 137 vitiligo patients who received PUVA therapy(using oral 8-MOP) for more than six months in our department between March 1990 and June 1996. Resulte . The results are summarized as follows 1. Among 137 cases of vitiligo, the numbers of male and female patients were 40(29.2%) and 97(70.8%) respectively. The mean age at the start of PUVA was 39 years and the mean duration of treatment was 31 months. There were no patients with skin type I and II. Forty cases had skin type III, 68 skin type IV, 29 skin type V. 2. Acute side effects were nausea(35.8%), pruritus(32:8% ), erythema(30.7%), headache(21.2 %), dizziness(18.8%), burning sensation(16.8%), fatigue(8.8%) and the Koebner phenomenon(1.5 %). These are all reversible and disappear on discontinuation of treatment.. 3. PUVA-induced pigmentary changes were PUVA lentigines(39 cases;26.3%), hypopigmented confetti macules(36 cased;26.3%), mottling(31 cases,22.6%), nail pigmentation(8 cases,5.8%) and PUVA keratosis(7 cases;5.1%) 4. The mean cumulative UVA dose was 1833J/cm and the mean number of treatments was 171. In the high-dose group and the patients who had the most number of treatments, we observed an increased number of patients with PUVA lentigiens and mottling. However, no relationship was observed between the development of PUVA-induced pigmentary changes and sex or age at the start, of PUVA or in relation to skin type. 5. We did not see any patients with melanoma, nonmelanoma skin cancer or systemic cancer. Conclusion : Long term exposure to PUVA significantly increases chronic clinical side effects of PUVA. Therefore carefuI clinical follow-up of patients who receive long term PUVA therapy in necessary. This risk shoud be considered in selection of treatment. (Kor J Dermatol 1997;35(4): 684-692)

의치 장착 노인과 임플란트 장착 노인의 저작능력 및 삶의 질 비교 연구

김영숙,전보혜,Kim, Young-Sook,Jun, Boe-He 한국치위생학회 2011 한국치위생학회지 Vol.10 No.2

Objectives : The purpose of this study was to determine the effects of oral health on the quality of life of elderly patients with dentures or implants. It is our intention that through this analysis we can aid seniors in making the choice between dentures or implants, considering future masticatory function and the likely effects of this decision on the patients quality of life. Methods : This survey was conducted between November 1, 2010 and November 25, 2010, in Kyunggi-Do, whasung city. The research was carried out at one public health center, among elderly patients. 105 seniors were surveyed using the direct interviewing method, oral health related quality of life was measured by the Philadelphia Geriatric Center(PGC) Morale Scale. Collected data was examined using the SPSS 13.0 program, using frequency, mean, and standard deviation analysis, T-test and $x^2$ test. Results : The number of residual teeth for patients who have implants was $20.45{\pm}5.85$, while among those patients with dentures the number was considerably lower, $8.11{\pm}7.66$(p<0.05). The results of patients masticatory function, was $28.13{\pm}2.40$ for those with implants, and $25.35{\pm}4.15$ with dentures. The results were better for those with implants. Among implant patients overall satisfaction was rated $25.21{\pm}3.63$, higher than for those with dentures $20.20{\pm}6.79$(p<0.05). Quality of life was scored higher for patients with implants($12.76{\pm}2.61$) compared to those with dentures($10.47{\pm}3.52$)(p<0.05). The subjects masticatory function was highly related to the number of remaining teeth and greater masticatory function had a positive effect on patients quality of life and life satisfaction. Conclusion : Dental treatments such as dentures and implants will have a great influence on patients quality of life and life satisfaction. Implants can improve the oral health related quality of life and satisfaction and the general quality of life more than denture therapy.