The Apolipoprotein A1 polymorphisms were associated with decreased risk for metabolic syndrome in Koreans

김영리,홍승호 한국유전학회 2015 Genes & Genomics Vol.37 No.10

Apolipoprotein A1 (ApoA1) plays an important role in reverse cholesterol transport. The functional abnormality of ApoA1 is known to be the major risk for lipid disorders or cardiovascular diseases. In this study, we examined the associations of two ApoA1 polymorphisms (XmnI and MspI/rs670) with the Korean patients with metabolic syndrome (MetS). A total of 835 subjects were investigated, which includes 320 patients with MetS and 515 healthy controls. Genotyping of the ApoA1 polymorphisms was analyzed by conventional polymerase chain reaction and digestions with restriction endonucleases. The mutant genotypes of the XmnI and MspI polymorphisms were associated with the decreased risk of MetS. The X1-A and X2-G haplotypes for the XmnI and MspI polymorphisms were also protective to susceptibility for MetS. The XmnI and MspI polymorphisms showed significant associations with DBP (P = 0.047) and body mass index (P = 0.037) among MetS risk factors, respectively. Stratified analysis for MetS risk components was significantly associated between the XmnI polymorphism and blood pressure. Based on these results, the variants and haplotypes of the ApoA1 polymorphisms may be protective against MetS susceptibility in Koreans.

Associations of MTRR and TSER polymorphisms related to folate metabolism with susceptibility to metabolic syndrome

김영리,홍승호 한국유전학회 2019 Genes & Genomics Vol.41 No.8

Background Hyperhomocysteinemia is a potential risk factor for the development of metabolic syndrome (MetS). Among genes involved in homocysteine metabolism, polymorphisms of methylenetetrahydrofolate reductase (MTHFR) gene are known to be associated with MetS incidence. However, effects of polymorphisms of other folate metabolism-related genes on MetS susceptibility are not well known yet. Objective This study was to determine whether methionine synthase (MTR) 2756A > G, methionine synthase reductase (MTRR ) 66A > G, and thymidylate synthase enhancer region (TSER) 2R/3R polymorphisms might be associated with risks of MetS development in the Korean population. Methods Genotype analysis of the three polymorphisms was performed for a total of 483 subjects including 236 MetS patients and 247 unrelated healthy controls using polymerase chain reaction-restriction fragment length polymorphism technique. Results The present study revealed that MTRR and TSER polymorphisms were associated with susceptibility to MetS. Several genotypes and allele combinations from the three polymorphisms were also related to the MetS prevalence. When polymorphism data were stratified according to the risk components of MetS, MTR polymorphism was significantly associated with an increased risk of MetS in subjects with systolic blood pressure < 132.7 mmHg (AOR 1.842, 95% CI 1.039–3.266, P = 0.037) and fasting blood glucose level < 106.3 mg/dL (AOR 1.772, 95% CI 1.069–2.937, P = 0.027). MTRR polymorphism was significantly associated with a decreased risk of MetS in subjects with triglyceride level < 216.3 mg/dL (AOR 0.616, 95% CI 0.399–0.951, P = 0.029). To the best of our knowledge, this is the first to provide reliable evidence about the association of other folate metabolism-related gene polymorphisms besides MTHFR with MetS susceptibility and its risk factors. Conclusion Results of this study suggest that MTRR and TSER polymorphisms might be potential genetic markers for the risk of MetS development in Korean population.

國語 特殊助詞의 意味

金映利 제주대학교 사범대학 국어교육과 국어교육연구회 1988 白鹿語文 Vol.5 No.-

누에 수 번데기의 성분 분석

김영리,박정식,조춘구,홍세흠,류강선 한국피부미용향장학회 2010 한국피부미용향장학회지 Vol.5 No.3

Evaluation of HER2/neu Status by Real-Time Quantitative PCR in Breast Cancer

김영리,최종락,송경순,정우희,이희대 연세대학교의과대학 2002 Yonsei medical journal Vol.43 No.3

Over-expression of the human epidermal growth factor receptor-2 (HER2/neu) has been observed in many cancers, and is associated with a poor prognosis. Recent adjuvant treatment with anti-HER2 monoclonal antibodies in breast cancer has increased the demand for an evaluation of the HER2/neu status in breast cancer. The aim of this study was to investigate the HER2/neu status in breast cancer by a real-time quantitative polymerase chain reaction (PCR) method using LightCycler (Roche Diagnostics, Mannheim, Germany). DNA samples from the fresh tumor tissues of 27 patients with breast cancer were analyzed in parallel using immunohistochemistry (IHC) and the other prognostic parameters including estrogen receptor, progesterone receptor, cytokeratin, and DNA ploidy. Ten (37%) out of 27 cases tested were positive for HER2/neu, while 16 (73%) out of 22 tested positive through an IHC study. The correlation between the DNA aneuploidy and the positive results for HER2/neu were only observed using the real-time PCR method (p<0.05). There was no significant correlation between the HER2/neu status and the S-phase fractions of the DNA ploidy or other parameters. This study demonstrated that there is marked discordance in the results for the HER2/neu status according to the various methods used. Real-time quantitative PCR for HER2/neu appears to be clinically useful due to its simplicity and ability to produce rapid results.

반응성염료와 양모단백질간의 가교효과가 양모의 성질에 미치는 영향

김영리,유효선 한국섬유공학회 1994 한국섬유공학회지 Vol.31 No.2

In this study the effect of reactive dyeing on chemical properties of wool was investigated. Dyes featuring two reactive groups or one bifunctional stoup applied to wool, and dyeing was carried out with or without ammonia aftertreatment. The purpose of this investigation was to test the effect of the number and position of reactive groups on fixation ratio, urea-bisulphite solubility(UBS) and swelling in formic acid, on the physical properties of dyed wool fabric. The main results obtained from this study were as follows: 1. Molecular size, configuration and number of reactive group afreet the exhaution ratio and fixation ratio of the dye. The highest fixation ratio was attained with Lanasol Red G which they possesses two reactive groups. 2. In the case of a-bromoacrylamide dye, the urea bisulphite solubility of the dyed wools indicated significant dependence on the amount of dye applied and the number of reactive groups in the dye used. These results suggest that crosslinking of the peptide chains by the dye with both bifunctional group occurs. 3. The wool dyed with bifunctional reactive dyes showed a slight improvement in tensile strength and breaking elongation compared to the blank-dyed fabrics. 4. In the case of alkali after-treatment of the reactive dyeing, the UBS and mechanical properties of wool clearly indicated that the reactive dyes protected fibres against alkali degradation. This is ascribed to the fact that the $\varepsilon$-amino groups in the Iysine which are responsible for alkali degradation are partially blocked by reactive dyes.

RhD 음성 한국인에서의 염기서열특이-중합효소 연쇄반응법을 이용한 RHD 유전자검사법

김영리,조윤정,김영태,김상배,서동희,황유성,조남선 대한임상병리학회 2000 대한임상병리학회지 Vol.20 No.1

Gender-specific association of polymorphisms in the 50-UTR and 30-UTR of VEGF gene with hypertensive patients

김영리,홍승호 한국유전학회 2015 Genes & Genomics Vol.37 No.6

Vascular endothelial growth factor (VEGF) is one of the most potent angiogenic factors. VEGF plasma levels have been reported to be significantly elevated in the patients with hypertension. This study aims to elucidate the associations of the VEGF -634G[C and 936C[T polymorphisms with the hypertensive patients in Korea. Genotyping of the VEGF polymorphisms was carried out by polymerase chain reaction and endonuclease digestion. The C allele of the VEGF -634G[C polymorphism and T allele of the 936C[T polymorphism were significantly higher in the hypertensive patients than in the normotensive controls. When the data of the genotype were stratified by gender, the association was gender-specific. We also found the associations of the haplotypes, composed of the VEGF -634G[C and 936C[T polymorphisms, with hypertensive group. Dominant models of -634G[C and 936C[T polymorphisms were associated with risk components of hypertension, according to the stratification analysis. Thus, this study demonstrates that the VEGF -634G[C and 936C[T polymorphisms may provide important genetic markers in identifying people at risk for hypertension. So far, association studies of VEGF gene polymorphisms with the hypertensive patients were rare. Therefore, further evaluation is required in other ethnic or racial populations with large samples to confirm our data.

Early Experience Using a Left Atrial Appendage Occlusion Device in Patients with Atrial Fibrillation

김영리,박희남,정보영,온영근,심지영,이문형,김영훈 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.1

Purpose: Atrial fibrillation (AF) is one of the major risk factors for ischemic stroke, and 90% of thromboembolisms in these patients arise from the left atrial appendage (LAA). Recently, it has been documented that an LAA occlusion device (OD) is not inferior to warfarin therapy, and that it reduces mortality and risk of stroke in patients with AF. Materials and Methods: We implanted LAA-ODs in 5 Korean patients (all male, 59.8±7.3 years old) with long-standing persistent AF or permanent AF via a percutaneous trans-septal approach. Results: 1) The major reasons for LAA-OD implantation were high risk of recurrent stroke (80%), labile international neutralizing ratio with hemorrhage (60%), and 3/5 (60%) patients had a past history of failed cardioversion for rhythm control. 2) The mean LA size was 51.3±5.0 mm and LAA size was 25.1×30.1 mm. We implanted the LAA-OD (28.8±3.4 mm device) successfully in all 5 patients with no complications. 3) After eight weeks of anticoagulation, all patients switched from warfarin to anti-platelet agent after confirmation of successful LAA occlusion by trans-esophageal echocardiography. Conclusion: We report on our early experience with LAA-OD deployment in patients with 1) persistent or permanent AF who cannot tolerate anticoagulation despite significant risk of ischemic stroke, or 2) recurrent stroke in patients who are unable to maintain sinus rhythm. Purpose: Atrial fibrillation (AF) is one of the major risk factors for ischemic stroke, and 90% of thromboembolisms in these patients arise from the left atrial appendage (LAA). Recently, it has been documented that an LAA occlusion device (OD) is not inferior to warfarin therapy, and that it reduces mortality and risk of stroke in patients with AF. Materials and Methods: We implanted LAA-ODs in 5 Korean patients (all male, 59.8±7.3 years old) with long-standing persistent AF or permanent AF via a percutaneous trans-septal approach. Results: 1) The major reasons for LAA-OD implantation were high risk of recurrent stroke (80%), labile international neutralizing ratio with hemorrhage (60%), and 3/5 (60%) patients had a past history of failed cardioversion for rhythm control. 2) The mean LA size was 51.3±5.0 mm and LAA size was 25.1×30.1 mm. We implanted the LAA-OD (28.8±3.4 mm device) successfully in all 5 patients with no complications. 3) After eight weeks of anticoagulation, all patients switched from warfarin to anti-platelet agent after confirmation of successful LAA occlusion by trans-esophageal echocardiography. Conclusion: We report on our early experience with LAA-OD deployment in patients with 1) persistent or permanent AF who cannot tolerate anticoagulation despite significant risk of ischemic stroke, or 2) recurrent stroke in patients who are unable to maintain sinus rhythm.

일 간호대학생의 국제보건 간호역량과 셀프 리더십의 관계

김영리,홍은희 한국컨설팅학회 2022 컨설팅융합연구 Vol.2 No.3