기관지 내시경으로 초기에 제거할 수 없었던 기도 이물 : 2례 보고

김연수,남승연,곽병곤,장우익,박경택,김창영,류지윤,Kim, Yeon-Soo,Nam, Seung-Yeon,Kwak, Byeong-Gon,Chang, Woo-Ik,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2

Foreign body aspiration is a cause of the accidental death at home. Therefore, early intervention and proper management is important. A bronchoscopy is indicated whenever there is a suggestive history and medical opinion. Occasionally, foreign body removal with bronchoscopy may be fail. But, on the situation, there is no definite recommended standard management. We experienced two cases of bronchial foreign body could not be removed with bronchoscopy at first intervention. The one was diagnosed too late. Endobronchial granulation tissue and edema made it impossible to find the foreign body at first bronchoscopy. After steroid and antibiotic therapy, foreign body could be removed with secondary bronchoscopy. Another was bronchial foreign body jammed tightly bronchus intermedius. Even after medical therapy, patient got aggravated. So foreign body was removed with bronchotomy.

소 유방염 유래 Staphylococcus aureus의 AFLP 지문분석

김연수,김상균,황의경,Kim, Yeon-soo,Kim, Sang-kyun,Hwang, Eui-kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2

Amplified fragment length polymorphism(AFLP) technique is based on the polymorphism detection through selective PCR amplification of restriction fragments from digested genomic DNA and thus includes the procedures of the total DNA digestion by endonucleases, ligation of adapters to the ends of the fragments, and following the selective amplification of the restricted DNA fragments. This study were aimed to : (1) determine the genetic variability of S aureus strains, (2) estimate genetic diversity within and among these strains, (3) compare phylogenetic relationships among these strains as genetic markers using AFLP techniques. Genomic DNA was digested with a particular combination of three restriction enzymes with specific recognition sites and the DNA fragments were ligated to restriction specific adapters and amplified using the selective primer combinations. In the S aureus strain, the number of scorable AFLP bands detected per each primer combination varied from 29 to 102, with an average of 61.59 using 27 primer combinations. A total of 1,663 markers were generated, 904 bands of which were polymorphic, showing a 33.48% level of polymorphism with these primer combinations. Among the primer combinations, E02/T02, E02/T03, E04/H02, E02/T01 and E04/H03 primer combinations showed a high level of polymorphism with 0.78, 0.76, 0.74, 0.71 and 0.70, respectively. But T03/H01, E01/T02 and E01/T03 primer combinations showed a low level of polymorphism with 0.38, 0.37 and 0.15, respectively, Therefore, the former primer combinations will be the most effective for AFLP analysis of S aureus. In SA1 sub-types the level of polymorphism of S aureus KCTC 1927 was similar to that of S aureus CU 01(0.825) and higher than those of other strains such as S aureus CU 02 (0.715), S aureus KCTC 2199(0.625), S aureus KCTC 1916(0.607) and S aureus KCTC 1621 (0.553). In SA2 sub-types the level of polymorphism of S aureus CU 07 was similar to that of S aureus CU 08(0.935) and higher than those of both S aureus CU 04(0.883) and S aureus CU 05(0.883) and lower than those of S aureus CU 03(0.583). In SA3 subtypes the level of polymorphism of S aureus CU 11 was similar to that of S aureus CU 12(0.913) and lower than that of S aureus CU 15(0.623). The results proved that AFLP marker analysis of S aureus strain could be used to study the epidemiology of mastitis and in addition, common genotype in geographic region could be useful for the development of an effective vaccine or DNA marker for easy diagnosis of mastitis caused by S aureus infection.

Functions of Early Palindrome Domain in SV40 DNA replication

김연수,하정실,강현삼,Kim, Yeon-Soo,Ha, Jeong-Sil,Kang, Hyen-Sam 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3

SV40 Replication core origin에 존재하는 early palindrome의 구조와 기능에 대해 알아보기 위해 SV40 origin을 포함하는 0.8kb DNA fragment를 filamentous phage M13 DNA에 cloning시킨 후 oligonucleotide-directed site-specific mutagenesis에 의해 early palindrome이 이룰 수 있는 hairpin 구조 중 stem 부분이 파괴된 mutant들과 base의 치환은 있으나 stem 부분은 다시 회복된 mutant들을 만들었다 in vivo replication assay를 위해 mutation이 일어난 origin 부위를 pAT153 vector로 subcloning하여 COS-1 cell에 transfection함으로써 replication level을 측정하였다. 그 결과 early domain의 inverted repeats는 fuctional cruciform structure를 형성함으로써 SV40 replication에 관여하는 것은 아니라는 것을 알 수 있었다. To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.

Hepatitis B Virus의 표면항원 유전자의 Cloning과 발현에 관하여

김연수,강현삼,Kim, Yeon-Soo,Kang, Hyen-Sam 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.1

사람의 피로부터 hepatitis B virus(HBV)를 순수 분리한 뒤 endogenous DNA polymerase 반응에 의해 완전한 double strand HBV DNA를 얻었다. 이 circular double stranded DNA를 BamHI으로 절단한 둬 pBR322에 삽입시켜 HBV의 total genome을 포함하는 재조합 plasmid pHBV-315를 제조하였다. cloning된 viral genome을 분석하기 위해 pHBV-315의 제한효소 지도를 작성하고 일부 염기 서열을 비교 조사하였다. 이 결과로부터 subtype adr HBsAg 유전자가 포함된 HBV DNA가 cloning 되었음을 알 수 있었다. pHBV-315로 부터 pHBV-15를 재조합하여 LMTK-세포와 E. coli에서 HBsAg 유전자의 발현을 조사하였다. 그 결과 LMTK-세포에서는 HBsAg이 검출되었고 E. coli에서는 검출되지 않았다. Dane particles were purified from human plasma and the DNA containing the single strand region was filled-in by endogenous DNA polymerase. Recombinant plasmid pHBV-315 was constructed by inserting the total HBV DNA into pBR322 DNA. Restriction enzyme map and nucleotide sequence of pHBV-315 suggested that the HBsAg subtype of the cloned HBV DNA is adr and how HBsAg gene is arranged in pHBV -315. pHBV -15 was constructed to direct the expression of HBsAg gene. When LMTK-cells and E.coli were transformed by pHBV-15, HBsAg was produced in LMTK-cells but not in E.coli.

DNA Amplified Fingerprinting 기법을 이용한 Salmonella pullorum과 Salmonella gallinarum의 다형성 비교 분석

김연수,김상균,송원철,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Song, Won-Chul,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

This study was performed to detect the Salmonella genus-specific DNA marker for comparing of polymophisms between S pullorum and S gallinarum by using PCR amplified techniques. A total of ten primers were used to detect DNA polymorphisms from S pullorum and S gallinarum. The number of DAF bands detected per each primer varied from 26 to 45, with an average of 32.7 using 10 primers. A total of 327 DAF bands were generated and among them 123 bands were polymorphic(37.6%). These DNA amplified fingerprinting(DAF) specific bands for S pullorum and S gallinarum were observed from all primers. For S pullorum, GEN 60-04, GEN 70-04 and GEN 70-03 primers showed a high level of polymorphism with 0.79, 0.70 and 0.57, respectively. But GEN 60-05 primer did not show a level of polymorphism. For S gallinarum, GEN 70-03, 60-04, 60-07, 70-05 and 70-04 primers showed a higher a low level of polymorphism from 0.16 to 0.28. Each five strains of S pullorum and S gallinarum were isolated from chickens showed typical clinical signs related with infection of pullorum disease or fowl typhoid at commercial chicken farms. DNA markers of these strains produced by GEN 70-04, GEN 70-05 and GEN 70-08 showed significant difference of band patterns between S pullorum and S gallinarum. These DNA markers could be used for comparison of DNA marker polymorphism between S pullorum and S gallinarum as well as rapid diagnosis of fowl typhoid and pullorum disease of domestic fowls.

좌측 주기관지 근위부에서 발생한 무기폐를 동반한 횡문근 육종의 수술 치료 -1례 보고-

김연수,장우익,허진원,박시영,장선희,박경택,김창영,류지윤,조성준,Kim, Yeon-Soo,Chang, Woo-Ik,Huh, Jin-Won,Park, See-Young,Chang, Sun-Hee,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon,Cho, Seong-Joon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2

Treatment choice for primary pulmonary sarcoma is complete surgical resection. A 69 year old man developed dyspnea due to left lung atelectasis. There was endobronchial tumor completely obstructing the left main bronchus. The tumor was resected completely by main bronchial resection via a left thoracotomy incision, and diagnosed as leiomyosarcoma. Bronchoscopy and computed tomography in 6 months after operation, there was no evidence of recurrence.

강원도 사육 한우의 간질 감염실태

김연수,김상균,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

A field survey of fascioliasis of Korean native cattle raising and raised in specialized commercial breeding farms and local farms in Kangwon province using both intradermal test and sedimentation technique for feces was carried out from November to December, 1996. Fecal samples were taken from fascioliasis positive cattle by the intradermal test for the fecal examination. Liver tissues were randomly collected from an abattoir for histopathological examination of liver fluke infection in cattle. The results are as follows. 1. By the intradermal test for a total of 211 cattle raising in both Wonju and Wheongsung, Kangwon province, 60 heads(28.4%) showed positive reaction. Among 60 positive cattle, eggs of Fasciola hepatica were found from 51 heads(85.0%) by sedimentation technique. 2. According to the cattle raising areas, the positive rates by the intradermal test were 26.7%(20 out of 75 heads) in Wonju and 29.4%(40 out of 136 heads) in Wheongsung. 3. According to the age of cattle examined, the positive rates by the intradermal test in 1~3, 4~6 and 7~10 years old were 11.7%(7 out of 100 heads), 68.3%(41 out of 93 heads) and 20.0%(12 out of 18 heads), respectively. 4. The overall infection rates of fluke larvae from the slaughtered cattle at an abattoir in Wonju was 24.7%(37 out of 150 heads). In histopathology, liver lesions were observed such as inflammation with infiltration of eosinophils, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissues, calcification and abscess formation.

협력치안을 통한 4대 사회악 근절방안에 관한 연구- 익산시와 익산경찰서의 사례를 중심으로 -

김연수,Kim. Yeon Soo 한국범죄심리학회 2015 한국범죄심리연구 Vol.11 No.1

이 연구는 현 정부의 가장 큰 관심사인 4대 사회악 근절방안으로 협력치안을 통한 접근을 제안하며, 협력치안의 모델을 가장 잘 보여주고 있는 익산시와 익산경찰서의 사례를 중심으로 4대 사회악 근절방안을 검토하였다. 전국적으로 4대 사회악을 근절하기 위한 다양한 노력이 시도되고 있지만, 근본적인 문제해결을 위해서는 지역사회경찰활동, 치안서비스 공동생산이론, 제3자 경찰활동 등에서 제안하는 바와 같은 지역사회 내 자원의 적극적인 협력과 지원을 토대로 하는 협력치안의 형태가 바람직하다. 특히, 4대 사회악이 비록 중앙정부의 의제설정에 따른 것이라는 특수성이 있으나, 성폭력, 가정폭력, 학교폭력, 불량식품이라고 하는 사회문제가 경찰이나 정부기관의 노력으로만 해소될 수 있는 문제가 아니라는 점에서 시민의 적극적인 관심과 지원이 필수적이다. 이 연구는 4대 사회악 근절을 위해 익산시가 ‘4대 사회악 근절 추진본부’를 설립하였고, 익산경찰서가 ‘맞춤형 통합지원 네트워크’를 구축하였지만, 일정부분 한계가 있다고 본다. 연구를 통해 확인된 한계를 극복하기 위해 다음과 같이 개선방안을 제안하였다. ① 지역자원 활용 강화, ② 지역자원의 추가발굴, ③ 4대 사회악 유형세분화와 대응정책 개발, ④ 정보공유를 통한 범죄예방전략 수립 등을 제안한다. This study proposes an approach to cooperative policing in the best interest of the countermeasures of the current government to eradicate four social evils. It was investigated a model of cooperative policing in the case of Iksan city and Iksan police department. Various efforts have been attempts to eradicate four social evils. However, in order to solve the fundamental problems in the form of cooperative policing, such as that proposed a community policing, police service co-production theory, and third party policing, that is based on the active cooperation of the resource in the community is desirable. Active interest in social issues(sexual violence, domestic violence, school violence, and food violations) is essential for the support of the citizens in that is not a problem that can be solved only by the police or government agency efforts. This study looks that build of ‘Promotion Headquarters for the Eradication of 4 Social Evils in Iksan city’ and ‘Integration Support Network of Iksan Police Department’ is a certain part of the limits for the eradication of 4 social evils. To overcome the limitations identified through the study suggest improvements as follows: ① strengthen local resource utilization, ② additional excavation of local resources, ③ 4 evils’ type specification and response policy development, ④ crime prevention strategies through information sharing.

Simian Virus 40 복제 원점 부위의 특정 염기서열의 기능

김연수,하정실,강현삼 ( Yeon Soo Kim,Jeong Sil Ha,Hyen Sam Kang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3

To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.

소 성장호르몬 cDNA 및 유전자의 분리와 대장균에서 cDNA 의 발현

김연수,정신우,김병한,이명희,설동섭,강현삼 ( Yeon Soo Kim,Shin Wu Jeong,Byung Han Kim,Myung Hee Lee,Dong Sub Sul,Hyen Sam Kang ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1

cDNA was prepared from poly(A)^+mRNA isolated from pituitaries and cloned in E. coli. It contains the coding sequence for bovine growth hormone(bGH), The cloned bGH cDNA was confirmed by colony hybridization, restriction enzyme mapping, and nucleotide sequencing. The cloned eDNA was modified to construct the plasmid that expresses the bovine growth hormone in bacteria. A genomic gene of bovine growth hormone was also cloned using the bGH cDNA as a probe.