W/O 마이크로 에멀젼법에 의한 SiO_2 나노 입자의 제조에서 반응조건이 크기 및 입도분포에 미치는 영향

김순회,김기도,송건용,김희택 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.1

물, 유기용매(cyclohexane), 수산화암모늄(NH_4OH) 그리고 계면활성제(NP-5)로 이루어진 W/O 마이크로 에멀젼 용액에 TEOS를 첨가한 후 반응시켜 실리카 나노 입자를 합성하였다. 반응 조건으로는 반응시간, R값(=물/계면활성제)그리고 암모니아의 농도 변화에 따른 입자의 크기 및 입도 분포를 살펴보았다. 실험 결과 반응 시간은 48 시간 후에 나노 크기의 실리카 입자가 생성되었고, R값의 경우에는 25일 때, 그리고 암모니아의 농도는 0.1 M일 때 나노 입자를 제조하기 위한 최적 조건임을 알 수 있었다. 그리고 이러한 최적 조건에 의해서 얻어진 실리카 입자는 좁은 입도 분포(<±3.0%)를 지닌 약 10-30mm의 크기임을 확인할 수 있었다. To synthesize silica anopaticles, water, organic solvent(cyclohexane), ammonium hydroxide, and surfactant (NP-5) were used as a microemulsions, and then TEOS(TetraEthylOrthoSillicatr)as a precursor of silica was added to the solution. The effect of reaction time, R(=water/surfactant) value, and ammonia concentration on particle size and size distribution was observed. As a result, the sillica particles with nano size were prepared at the condition of 48 hrs reaction time. It was also found that the optimal conditions for R value and ammonia concentration were 25 and 0.1 M, respectively. Therefore, the silica nanoparticles(10-30mm) with narrow size distribution(<±3.0%) were formed by the above optimal conditions.

輸膽管 分泌異狀詩 膽囊上皮細胞의 變化에 對한 組織化學的 및 電子顯微鏡的 觀察

金淳會,李熙來 梨花女子大學校 韓國生活科學硏究院 1979 韓國生活科學硏究院 論叢 Vol.22 No.-

담낭상피세포의 생태를 이해하는 실험의 일환으로 형태학적으로 담낭상피세포를 관찰하기 위하여 집토끼를 정상군과 담관결찰 3일, 7일, 14일 및 21일군으로 구분하여 각 군에 5마리씩 배정하였다. 정상군과 담관결찰군 동물에서 일정부위의 담낭조직을 절취하였고, 광학현미경 관찰은 10% 중성 fromation액에 고정, hematoxylineosin(H-E), Periodic acid schiff 반응 - hematoxylin(PAS-H) 및 alcain blue pH2.5(AB 2,5) 염색을 시행한 표본으로, 전자현미경적 관찰은 2.5% glutaraldehyde-2% paraformaldehyde(phosphate buffer pH 7.3) 및 1%osimium tetroxide(phosphate buffer pH 7.3)에 고정, Epon 812에 포매, uranyl acetate와 lead citrate로 염색한 표본으로 관찰한 결과 다음과 같은 결론을 얻었다. (1) 담관결찰 집토끼의 담낭상피세포는 첨부세포질 내에 점액질이 증가하였다. (2) 대부분의 담낭상피세포, 늑히 간상세포는 세포간극이 협소해지고 rough surfaced endoplasmic reticulum 및 Golgi complex가 확장되고, 분비과립은 증가하고, pinocytotic vesicles는 감소하는 경향을 보였다. (3) 위의 여러 소견으로 미루어 담관결찰로 인하여 담낭상피세포는 일시적으로 합성 및 분비기능은 항진되고 흡수기능은 저하되는 것으로 생각된다. This experiment was performed to study the morphological changes of the gallbladder epithelial cells of rabbits afte bile duct ligation. Healthy male rabbits weighing about 2kg were used as experimental animals and divied into normal and bile duct ligated groups. Bile duct ligated rabbits were sacrificed on the 3rd, 7th, 14th and 21st day after the operation. For light microscopy, mucosal specimens from the gallbladder of the experimental rabbits were fixed in 10% neutral formalin solution, embedded in paraffin wax, sectioned at a thickness of 5㎛, and stained with hematoxylin-cosin for usual histologic study, by periodic acid Schiff's reaction-hematoxylin and alcian blue pH 2.5 histochemical study. For electron microscopy, the tissues were fixed in 2.5% glutaraldehyde 2& paraformaldehyde solution (phosphate buffer pH 7.3) and the LKB 8800A ultratome. snd stained with uranyl acetate and lead citrate and observed by JEM - 100B electron microscope. The observed results were as follows : 1. After the bile duct ligation, the epithelial cells of gallbladder contained large amounts of mucosubstance in the apical cytoplasm. 2. Rod-shaped cells of the gallbladder epithelium showed narrowness of intercellular spaces, enlargement of rough surfaced endoplasmic reticulum and Golgi complex, increase of secretory granules and decrease of pinosynthesis vesicles by bile ductligation. 3. These findings imply that secretion and biosynthesis of rod-shaped cells are promoted by bile duct ligation, and absorption of the cells is suppressed under the same condition.

태생 및 신생 흰쥐의 위유문부 gastrin 세포에 대한 면역세포화학적 연구

金淳會,李熙來,李惠星 梨花女子大學校 韓國生活科學硏究院 1988 韓國生活科學硏究院 論叢 Vol.41 No.-

The structure and distribution of gastrin cells in the gastrointestinal tract of albino rats, Sprague-Dawley strain, have been clarified, but little have been studied on the ontogeny of the gastrin cells. The present study was attempted to examine the gastrin-immunoreactive cells in the pyloric antrum of the fetal and neonatal rats. In the 19-day-old fetuses the gastrin cells were identified in the epithelium of the pyloric antrum with the glands. In the early period of neonate the gastrin cells were gradually increased and most of the cells were located in the base of the pyloric gland. It imply that the gastrin cell may influence the gastric mucosal cellular proliferative activity during various stages of late fetal and early neonatal development.

韓國人胎兒 心臟發育에 關한 形態計測學的 연구

金淳會 이화여자대학교 한국문화연구원 1967 韓國文化硏究院 論叢 Vol.11 No.-

642 cases of the normal Korean fetal hearts from the middle and later stages of prenatal life have been detected by means of the morphometrial observations over 10 items. 1) Regardless the sex, the mean weight of the heart from the term of the pregnancy was reached at 15 times of that of the 5th lunar month. 2) Among the increase of value of the measurements during the described period, such as longitudinal, transverse and anteroposterior diameters, that of the transverse diameter was the greatest. The considerable difference between the longitudinal and transverse diameters at the 5th was practically minimized undetectably by the 10th lunar month. 3) Among the sizes of various large veins which are emptying into the heart, that of the inferior vena cava was the largest. The monthly growth rate of the superior vena cava, however, was the highest among them. 4) The size of opening of the foramen ovale was continuously grow until the 9th, and was regress thereafter. 5) The size of the opening of the left atrio-ventricular ostium was large during the described period in contrast to that of the right, but the size difference between the right and left was minimized at the 5th and 10th month. 6) Nevertheless the similarity of the monthly growth rates, the size of the aortic ostium was larger than that of the pulmonary ostium during the described period. 7) Throughout the measurements, the interventricular septum was the thickest, and was followed by the walls of the left ventricle, right ventricle, interatrial septum, right atrium and left atrium. Among the growth of walls, remarkable velocity of the growth of the right ventricle was substantiated to the similarity between the right and left ventricular walls by the 10th month. 8) Longer papillary muscles in the left ventricle was measured, while, the difference of the numbers was negligible. 9) In regard to the number of the chorda tendinea, none of the relationships between the number of the chorda tendinea and cusps in the developing hearts. 10) The numbers of the papillary muscle and chorda tendinea in the each ventricle were less during the later stage than the middle stages. 11) None of the sexual difference was found under a statistical support.

Paneth세포의 퇴행성 변화에 대한 전자현미경적 연구

金淳會,李熙來 梨花女子大學校 韓國生活科學硏究院 1978 韓國生活科學硏究院 論叢 Vol.21 No.-

Paneth세포의 생태를 규명하는 실험의 일환으로서 형태학적으로 Paneth세포의 퇴행성 변화를 관찰하기 위하여 집토끼를 정상군과 췌관결찰 7일군으로 구분하여 각 군에 5마리씩 배정하여 사용하였다. 정상군과 췌관결찰군 동물에서 일정한 부위의 회장 조직을 절취하였다. 광학현미경적관찰은 10% 중성 formalin액에 고정, hematoxylin-eosin염색을 시행한 표본으로서, 전자 현미경적 관찰은 2.5% glutaraldehyde-2% paraformaldehyde 및 1% osmium tetroxide로 고정, uranyl acetate와 lead citrate로 염색한 표본으로 관찰한 결론은 다음과 같다. (1) Paneth세포의 변성은 ribosome의 감소, rough surfaced endoplasmic reticulum의 확장, Golgi 복합체의 위축, lysosome의 증가, 분비과립의 전자 밀도 감소 등의 소견을 보였다. (2) 변성된 Paneth세포는 이웃 대식세포에 의하여 포식되는 경우도 있는 것으로 생각된다. The present investigation was undertaken to resolve the fate of the degenerative Paneth cells at the bottom of the small intestinal crypts of the rabbit. Healthy adult male rabbits weighing about 2kg body weight were divided into two groups: normal and pancreatic duct ligation. Pancreatic duct ligated animals were sacrificed on the 7th day after the operation. For electron microscopy small tissue pieces were fixed in 2.5% glutaraldehyde-2% paraformaldehyde solution (phosphate buffer pH 7.3) for two hours and postfixed in 1% osmium tetroxide (phosphate buffer pH 7.3) for one hour. The tissue were embedded in Epon 812, sectioned with Sorvall porter-blum MT-2B ultramicrotome, and viewed with Hitachi HU-11E-1 electron microscope. The observed results were as follows: 1. Degenerative Paneth cells showed a decrease of free ribosome, dilatation of rough surfaced endoplasmic reticulum, atrophy of Golgi apparatus, a decrease in the electron density of secretory granules, and an increase of lysosomal structures. These morphological changes were intensified by pancreatic duct ligation. 2. In some degenerative Paneth cells, a part of the cytoplasm extruded to the basement membrane and was surrounded by a cytoplasmic process of macrophage. These morphological findings imply that Paneth cells were possibly phagocytosed by macrophages during cell generation.

Vitamin A 過剩投與가 自鼠胎仔에 미치는 影響

金淳會,李洸鎬 梨花女子大學校 韓國生活科學硏究院 1970 韓國生活科學硏究院 論叢 Vol.4 No.-

An administration of excessive vitamin A has been recognized as a method useful for teratological study. A teratologic effect of hypervitaminosis A on rat embryos was fist reported by Cohlan. Giroud and Martinet, Kalter and Warkany have carried out experiments of a similar nature and confirmed and extended the study. As the animals and methods used in these studies were different, it is very difficult to compare these data each other. The authors carried out the experiments for the effects of hypervitaminosis A on developing rat embryos with attention to malformations with the following results. Pregnant female rats were injected intraperitoneally with vitamin A on the 7th or 9th or 11th day after gestation. Various anomalies were produced in treated rats with varing frequencies. Cleft palate, abnormal tail and microcephalias were most common among them.

Synthesis and growth mechanism of TiO₂-coated SiO₂ nano particles by W/O Microeulsion

김순회,김기도,김희택 한국공업화학회 2003 한국공업화학회 연구논문 초록집 Vol.2003 No.1

돼지 췌장 유래 엘라스타제의 항원성 시험

김순회,백남기,안병옥,이상득,이순복,김원배,양중익 A.R. Liss 1990 ENVIRONMENTAL MUTAGENS AND CARCINOGENS Vol.10 No.2

흰쥐 腦 嗅結節의 連接에 대한 電子顯微鏡的 硏究

金淳會 梨花女子大學校 韓國生活科學硏究院 1981 韓國生活科學硏究院 論叢 Vol.27 No.-

체중 250g 내외의 흰쥐를 사용하여 후결절의 구조를 전자현미경으로 관찰코자 하였다. 실험동물은 nembuthal 마취하에 1% paraform-aldehyde-2% glutaraldehyde(phosphate by-ffer PH 7.4) 고정액을 대동맥을 통하여 관류 고정하고, 후결절에서 절취돈 조직은 2% osmium tetroxide(phosphate buffer PH 7.4)에 2시간 후고정하였고, ethanol과 aceton series에서 탈수하고, Epon 812 포매하여 60˚C oven에서 3일간 중화시켰다. 초박절편은 uranyl acetate 와 lead citrate로 중염색하여 관찰하여 다음과 같은 소견을 얻었다. 1. 후결절 내의 연접은 연접소포와 크기아 형태, 축삭종말의 연접부위에 따라 6가지 형으로 구분되었다. 제Ⅰ형 : 작고 구형의 연접소포를 갖는 축삭 수상돌기연접 또는 축삭세포체연접(43%) 제Ⅱ형 : 작고 구형의 연접소포를 갖는 축삭 수상돌기소극연접(23%). 제Ⅲ형 : 장타원형 연접소포를 갖는 축삭수상돌기연접(23%). 제Ⅳ형 : 장타원형 연접소포를 갖는 축삭수 상돌기소극연접(2%). 제Ⅴ형 : 크고 구형의 연접소포를 갖는 축삭 수상돌기연접(2%). 제Ⅵ형 : 크고 구형의 연접소포를 갖는 축삭 수상돌기소극연접(2%). 2. 후결절이 연접 중 제Ⅲ, Ⅳ형은 선조체에 분포하는 같은 형의 연접에 비하여 훨씬 높은 비율을 차지하였다. 3. 후결절은 선조체에 비하여 축삭수상돌기 소극연접이 매우 낮은 비율로 나타났다. 위의 소견으로 미루어 후결절은 형태학적으로 선초체와 다른 구조를 갖는 부위라고 생각된다. The normal ultrastructur of rat olfactory tuvercle was investigated. The rats were perfused with 2% glutaraldehyde and 1% paraformaldehyde fixative in phosphate buffer at PH 7.4. Tissues from olfactory tuvercle were postfixed by 2%OsO_4 in phosphate buffer at PH 7.4, dehydrated in geaded ethanol and acteon, and embedded in Epon 812. The polymerization was carried out in oven at 60 C. The ultrathin sections were cut an a LKB ultratome and were contrasted witd uranyl acetate and lead citrate. Special attention during the observation was given to the different types of synapses. Six types of synapses with many characteristics, could be differentiated: Small round synaptic vesicles-axodendritic and axosomatic (43%), axpospinous (28%), elongated (flattened) vesicle-axodendendritic (23%), axospinous (2%) and large round vesicle-axodendritic(2%), axospinous (2%). By slassification of synapses, distribution of each synapse in olfactory tubercle differ greatly from the striatum. These observation imply that the olfactory tubercle is a different structure from the striatum.

1P-211 : Synthesis and growth mechanism of TiO₂-coated SiO₂ nano particles by W/O Microeulsion

김순회,김기도,김희택 한국공업화학회 2003 한국공업화학회 연구논문 초록집 Vol.2003 No.-