에세이 - 또 하나의 감옥

김순선,Kim, Soon Sun 대한건축사협회 2018 建築士 Vol.597 No.-

Plasma MicroRNA-21, 26a, and 29a-3p as Predictive Markers for Treatment Response Following Transarterial Chemoembolization in Patients with Hepatocellular Carcinoma

김순선,조효정,남지선,김현지,강대룡,원지환,김진우,김재근,이제희,김보현,이미영,조성원,정재연 대한의학회 2018 Journal of Korean medical science Vol.33 No.1

Background: We investigated an association between the levels of plasma microRNA (miRNA)-21, -26a, and -29a-3p and treatment outcomes following transarterial chemoembolization (TACE) in patients with hepatocellular carcinoma (HCC). Methods: A total of 198 patients with TACE-treated HCC were followed up for TACE refractoriness and liver transplantation (LT)-free survival. Pretreatment plasma miRNA-21, -26a, and -29a-3p levels were measured using quantitative real-time polymerase chain reaction. Results: During the mean follow-up of 22.3 (range, 0.7–79) months, 118 (59.6%) patients exhibited TACE refractoriness. Multivariate analyses showed that expression of a specific combination of miRNAs (miRNA-21 ≥ 2.5, miRNA-26a ≥ 1.5, and miRNA-29a-3p < 0.4) was associated with early TACE refractoriness (within 1 year; hazard ratio [HR], 2.32; 95% confidence interval [CI], 1.08–4.99; P = 0.031) together with tumor size (HR, 4.62; 95% CI, 1.50–14.21; P = 0.008), and macrovascular invasion (HR, 3.80; 95% CI, 1.19–12.20; P = 0.025). However, miRNA-21, -26a, and -29a-3p levels were not significantly associated with overall TACE refractoriness or LT-free survival. Additionally, large tumor size and macrovascular invasion were common predictive factor of overall TACE refractoriness and survival. Conclusion: Combination of plasma miRNA-21, -26a, and -29a-3p expression could predict early TACE refractoriness in patients with TACE-treated HCC.

우리나라 未婚母 福祉施設 現況에 관한 考察

김순선,민문기,정소연 원광대학교 사회복지학과 1989 圓光社會福祉學 Vol.4 No.-

공공 R&D 기관의 기술 상용화 과정에 관한 시스템 사고 분석

김순선,김동환 한국시스템다이내믹스학회 2007 한국시스템다이내믹스학회 춘계학술대회 발표논문집 Vol.2007 No.1

Nowadays almost nations try to promote the competition of nation through the development of national science technology. Also Korea has been engaging in this race of through public research & development institute. But recently the commercialization of R&D products appears a poor result in the royalty of technology according weak technology transfer toward IT small and medium-sized enterprises. Even If we have been trying to find the problems and causes, as yet it is true that we don`t solve clearly the problem, make a diagnosis about it. This paper analyzes the process of commercialization in public R&D research institute(ETRI: Electronics and Telecommunications Research Institute) through system thinking and researches an interrelationship among government agency, public R&D research institute, and enterprise. This one can find the fact that between research fund and R&D products, transfer technology and royalty, and enterprise`s operating profit and active technology transfer like adding technology development is a positive feedback loop. This positive feedback loop in the commercialization of public R&D research institute carries out very important role in examining problems in R&D product`s commercialization. Also this paper looks forward to being a guide in the commercialization of public R& research institute.

고래

김순선 시와세계 2004 시와세계 Vol.- No.8

복부 장기의 정상 초음파 해부학과 스캔법

김순선 대한내과학회 2019 대한내과학회 추계학술대회 Vol.2019 No.2

보육교사의 보육효능감과 직무만족이 영아의 상호작용에 미치는 영향

김순선,김진선 한국비전학회 2020 한국비전연구 Vol.2 No.-

본 연구는 영아반 보육교사의 보육 효능감과 직무 만족이 교사와 영아의 상호작용에 미치는 영향에 대하여 검증함으로써, 교사와 영아의 긍정적인 상호작용으로 영아의 건전한 성장발달과 보육의 질을 향상할 방안을 마련하는 데 목적이 있다. 본 연구의 목적에 따라 독립변수는 보육교사의 보육 효능감과 직무 만족, 종속변수는 영아와 교사의 상호작용으로 구성하였다. 보육교사의 직무 만족이 교사와 영아의 상호작용에 미치는 영향에 관한 분석 결과, 보육 효능감과 직무 만족은 교사와 영아의 상호작용에 긍정적인 영향을 미치는 것으로 나타났다. 이상의 연구결과에서 보육교사의 보육 효능감은 직무 만족을 통해 교사와 영아의 상호작용 수준을 향상할 수 있음을 확인하였다. The purpose of this study is to examine the effects of childcare efficacy and job satisfaction of infant teachers on interaction between teachers and infants, and to prepare a plan to improve healthy growth and development of infants and the quality of childcare through positive interaction between teachers and infants, The independent variables were child care efficacy and job satisfaction of infant teachers and the dependent variables were interaction between infants and teachers. The results of analyzing the effects of job satisfaction of childcare teachers on interaction between teachers and infants showed that childcare efficacy and job satisfaction had a positive effect on interaction between teachers and infants. The results of this study confirmed that childcare efficacy of childcare teachers can improve the level of interaction between teachers and infants through job satisfaction.

바이러스성 호흡기 감염의 역학 조사 및 각종 검체의 바이러스 동정률 비교

김순선,조영걸,김유겸,홍수종,김은순,우영대,주용규 대한감염학회 1999 감염 Vol.31 No.4

천연물이 간대사에 미치는 영향에 관한 연구(Ⅲ)

김순선,최기환,김학림,황인창,김동섭,신윤용,류항묵,장영섭 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

본 연구에서는 만성간염둥 간낄환 01방 및 치료제로서 오래전부터 널러 사용죄고 있으나 그 작용기전이 화실히 밝궈져있지 않은오미자에서 추출한 을hisandrin C의 합성 동족체, biphenf·1 dimethrt carboxylate(ODB)의 간장 보호자용 기전 연구의 일환으로 DDB를 시간(I~T일) 및 용량(25, 50, 100, 200mg/kg)별로 랫드에 투여한 훌 간장내 약물대사효소, CYPtAl과 CYPEBI mRNA 합량을 PolyrEerasff chain reation(PCR)밟법으로 측정하고 CfPIAl에 특이한 ethoxyruesorfin-o-deethylase(EROD)와 CYPaBt에 특이한 benzyloxyresorufin-o- dealkrlase(BROD) 활성을 측정한 결꽈, DDB가 CYPIAI mRNA 힘·량 및 EROD활성에는 영향을 미치지 않았으나 CYPfBl 유전자 발현을 유도시키고 CYPEBI에 특이한 fiROD 될성을 증가시켰다. DD8의 이러한 CYPEBI 유전자 발현유도 효과는 투여시간, 용량에 비례하여 3일, 200rag/kg투여시에 극대치를 나타내었으며 BROD찰상 역시 투여시간, 용량에 비례하여 증가하였다. 또한 DDB의 간보호 효과를 평가하기 위해 DDB를 용량(25, 50, 100,200mgJkg)별로 4일간 연속 길구투여하고 DDB 최종투여 3시간후 CCI,(0.3mJ/kg, i.p)를 처치하여 간독성을 유발 시킨 후, CYPfBl mRNA 함량과 BROD 활성을 조사하고 간조직 검사를 실시하였으며 혈청내 aminotransferase 꽉성 및 간소포체내 lipid perflxidation을 측정하였다. DOB 전투여는 CCI, 처치에 의왜 감소된 CYPBBI mRNA 함량자 BHOD 활성을 용량의콘적으로 증가시켰으며 CCI, 처치에 의해 증가된 혈청내 ALT 활성과 간 소포체 막의 lipid peroxidation을 감소시켰다. 혈청내 ALT 활성같 간 소포체 막치 lipid peroxidation에 근거하면 DDB는 CCI, 처치로 유발된 간독성에 럴호효롸를 나타내며 그 효과는 50mg/kg;투여시에 최대인 것으로 보인다. 반면, CCI, 처치에 의해 유발된 간 표면의 섬유화, 간세포 괴사 및 공포화 변성은 DDB 투여에 의해 통계적으로 유의성있는 개선 효과를보이지 않았다. 이상의 결과는 DDB가 CYP2린1 유전자 발현 및 BROD 활성을 시간 및 용량의존적으로 증가시킴을 나타내며 DDB의 free radical scavenger가능성을 제시한다. 미와 더불어 전년도 시험물질, 대황 및 마황의 환셩성분인 rhein, eptledrine과 황금의 지표성분인 baicalin이 간 대사 및 독성에 미치는 영향을 비교평가 하기 위해 rhein 및 ephedrinr·을 각각 20, 10mg/kg 용량으로 랫드에 4일간 복강주사하고 baicalin은 4mg/kg 용량으로 7일간 경구투여한 후, 간장내 약물대사 효소 CYPIAI과 CYPaBl 유전자 발현정도를 조사하고 애들에 특이한 EROD 와 BROD활성, 혈청내 ALT, AST 활성 및 간소포체내 지질과 산화물(rnslondialdehyde) 함량을 측정한 결과, ephedrine은 만성간염지표인 ALT/AS? ratio을 증가시키고(p (0.03) rhein은 MDA함량을 증가시켰는데,(p(0.01) 이는 대황 투여에 의한 간 소포체 막의 lipid peroxidation증가는 rhein에,마황에 의한 혈청내 ALT/AST ratio증가는 ephedrine에 기인함을 시사해주는 결과이며 금의 지표성분인 baicalin투여에 의해서는 혈청중 ALT 및 AST 활성이 재조군에 비해 유의적으로 감소되어(p(0.05) baiealin의 간보호 가능성을 제시하였다. Biphenyl diinethyl dicarboxylate(DDB) is a synthetic analogue of SchiBandrin C isolated from SchiBandrae Fructus and has been widely used for its hepatic protective effect. This study has beenundertaken to examine the effect of DDB on rat liver drug metaboliEing enzymes and to understand theprotective mechanism of D:DB against carbon tetrachloride(CCl4) -induced hepatic toxicity in rat liver.After DDB was administered into male rats for different periods of time(1 ~7 days) ana with differentdoses(25, 50, 100 and 200mg/kg), mRNA levels of CYPIAI and CYPaHl were measured by polymerasechain reaction (PCR) and also assayed the activities of CYP IAI specific ethoxyresorufin-o-deethylase(EROD) and CYPfBl sf)ecific benzyloxyresorufin-o-dealkylase (BROD) . Furthermore, rats werepretreated with DDB 25, 5() and 100 mg/ltg daily for 4 days, 3-hours after final treatrneBt on the 4thday, CCI, 0.3mf/kg was intraperitonially injected into the rats. Activities of serum aminotransferaseswere assayed and determirled lipid peroxidation(production of ualondialdehyde) in hepatic microsomeand done histopathological examination. DDB treatment resulted in increase in CYPaBl mRMA leveland BROD activity whereas there was no change in CYPIAI niRNA level and EROD activity. This ef-fect of DDB was time and dose dependent reaching maximal level by 3 day and 200mg/kg treatment.Based oi?'studies of serum ALT level and lipid peroxidation, D:DB SOmg/kg pretreatinent appeared themost effective protection against CCI,-induce4 hepatotoxicity. :However, histopathological examinationdid not significantly show any improving effect on balloning degeneration and necrosis caused by CCI,treatment. These results in.dicate that DDB stimulates CYPEBI ruRNA level and BROD activity in timeand dose dependent manner and suggest protective effect of DDB against CCI,-induced hepatotoxicitymight be exerted through free radical scavenging. In addition, we have also investigated the effects ofrhein, ephedrine and baicaBin on hepatic toxicity and metabolism in rat liver in order to compare withtheir natural products f·hick are Rhei Rhizoma, Ephedrae Hel·ba and Scutellariae Radix, respectively.After rhein 20 and ephedrine 10mg/kg were intraperitonially injected into the rats daily for 4 daysrespectively compared as bacaBin was treated orally for 7 days, the expression of CYPIAI and CYPfBlwere examined and tlleir dependent EROD and BROD activities were assayed. The levels of serumaainotransferases and hepatic rnaiondialdehyde(MDA ) were Beasured as well. Ephedrine treatment in-creased in CYPIAI specific EROD activity and the ratio of serum ALT/AST ratio, a marker of chronichepatitis(p <0.01). MDA level was enhanced by rhein treatmentfp < 0.01), but baicalin reduced serumALT and AST levels compared to control group (p < 0.05) .

구연 : B형간염의 만성화 및 간세포암의 발생과 type 1 interferon receptor 유전자 다형성

김순선,이다미,양민재,이명희,전수진,정재연,조성원 대한간학회 2009 Clinical and Molecular Hepatology(대한간학회지) Vol.15 No.3(S)