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녹차추출물의 Vibrio parahaemolyticus에 대한 항균활성
김선준(Seon-Jun Kim),황재호(Jae-Ho Hwang),한경호(Kyeong-Ho Han),조정용(Jeong-Yong Cho),마승진(Seung-Jin Ma),김두운(Duwoon Kim),문제학(Jae-Hak Moon),박근형(Keun-Hyung Park),김선재(Seon-Jae Kim) 한국차학회 2010 한국차학회지 Vol.16 No.2
Green tea extract was tested to determine its antimicrobial effects against the pathogenic bacteria Vibrio parahaemolyticus. The antimicrobial effect of green tea extract was evaluated by measuring the change in the concentration level of V. parahaemolyticus genomic DNA, which was obtained after treatment of green tea ethanol extract with neutral and acidic solvent-fractionation. Green tea extract of the neutral fraction showed concentration-dependent killing of V. parahaemolyticus, whereas green tea extract of the acidic fraction significantly inhibited the growth of V. parahaemolyticus at the minimum inhibitory concentration of 1,000~1,500 ppm.
녹차추출물의 넙치 어병세균, Edwardsiella tarda에 대한 항균 효과
김선준(Seon-Jun Kim),채송아(Song-A Chae),김두운(Duwoon Kim),황재호(Jae-Ho Hwang),한경호(Kyeong-Ho Han),오명주(Myung-Ju Oh),조정용(Jeong-Yong Cho),마승진(Seung-Jin Ma),문제학(Jae-Hak Moon),박근형(Keun-Hyong Park),김선재(Seon-Jae Kim) 한국차학회 2009 한국차학회지 Vol.15 No.3
녹차의 EtOH 추출물과 중성획분, 산성획분을 이용하여 넙치 에드워드병의 원인균 E. tarda에 대하여 항균활성을 측정하였다. EtOH 추출물과 중성획분 그리고 산성획분을 액체배지에 첨가하여 E. tarda를 10?CFU/mL 되게 접종하여 35℃에서 24시간 배양하여 추출한 genomic DNA의 감소로 항균성을 측정하였고, 600 nm에서 흡광도를 측정하여 생육억제활성을 측정하였다. E. tarda에 대한 녹차 추출물의 전체적인 항균력의 크기는 EtOH 추출물>중성획분>산성획분이었으며, EtOH 추출물 1500, 2000 ppm에서 E. tarda의 증식을 효과적으로 저해하였으며, 이에 대한 MIC와 MBC는 1000~2000 ppm 범위에서 측정되었다. Green tea extract was tested to determine the antimicrobial effects against Paralichthys olivaceus pathogenic bacteria, Edwardsiella tarda. The antimicrobial effect was evaluated by measuring the change of genomic DNA concentration level of E. tarda, which was obtained after treatment of green tea ethanol extract, EtOAc-soluble neutral and acidic solvent-fractionation, respectively. Green tea extract showed the concentration dependent killing against E. tarda, where the green tea extract significantly inhibited the growth of E. tarda in the minimum inhibitory concentration of 1000~2000 ppm and minimum bactericidal concentration of 1500~2000 ppm.
여성 결혼이민자들의 한국어 조음에 나타나는 음향음성학 특성 연구
조선희(Seon-Hui Jo),김현기(Hyun-Gi Kim),김선준(Sun-Jun Kim) 한국음성학회 2008 음성과학 Vol.15 No.3
The number of Asian women immigrants in Korea is getting bigger and it’s important to note that their communication problem in Korean causes not only the difficulty of adapting to Korean society but their children’s speech-language disorder. To date there is little research on their acoustics characters and articulatory errors. Therefore, this study focuses on acoustic characters and articulatory error patterns of the women immigrants from Japan and Philippine based on the theory of “contrastive analysis”. The subjects were 16 Japanese women immigrants(age: 42.5±4.4) and 14 Philippine women immigrants(age: 31.64±6.7) and control group consisted of 10 Korean women(age: 28.3±1.2). Speech and hearing of all subjects and control group were within normal limits. Speech samples were analyzed in a computer using CSL and data analysis was done on FFT widow for F1, F2, F3 of vowels and on wideband spectrogram for VOT of plosives and africatives. The results of this study were like this; For Japanese women immigrants, they had different articulatory patterns of /e/, /a/, /u/, /o/, /?/, /?/ from those of Koreans and showed articulatory errors on the fortis and aspirated sounds. The reason is Japanese has only two distinctive characters for plosives and affricates; voicing and voiceless. The Philippine women immigrants also showed the same error patterns as the Japanese women immigrants. Especially the errors on aspirated sounds were prominent because their mother tongue has no distinctive characters about aspirated sounds. For vowels, they showed errors of /a/, /o/, /?/.